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Protein

Chitinase A1

Gene

chiA1

Organism
Bacillus circulans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei204Proton donorCurated1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM12. Carbohydrate-Binding Module Family 12.
GH18. Glycoside Hydrolase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitinase A1 (EC:3.2.1.14)
Gene namesi
Name:chiA1
OrganismiBacillus circulans
Taxonomic identifieri1397 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi200D → E: No change in activity. 1 Publication1
Mutagenesisi200D → N: Decrease in activity. 1 Publication1
Mutagenesisi204E → D or Q: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 41Add BLAST41
ChainiPRO_000001190542 – 699Chitinase A1Add BLAST658

Structurei

Secondary structure

1699
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi40 – 43Combined sources4
Beta strandi45 – 51Combined sources7
Helixi52 – 55Combined sources4
Turni56 – 58Combined sources3
Helixi62 – 64Combined sources3
Helixi67 – 69Combined sources3
Beta strandi71 – 80Combined sources10
Beta strandi85 – 88Combined sources4
Beta strandi97 – 100Combined sources4
Beta strandi116 – 120Combined sources5
Helixi121 – 125Combined sources5
Beta strandi134 – 137Combined sources4
Helixi140 – 151Combined sources12
Beta strandi156 – 162Combined sources7
Beta strandi164 – 166Combined sources3
Helixi170 – 174Combined sources5
Helixi177 – 194Combined sources18
Beta strandi197 – 202Combined sources6
Beta strandi206 – 208Combined sources3
Helixi220 – 242Combined sources23
Beta strandi247 – 252Combined sources6
Helixi256 – 260Combined sources5
Helixi264 – 270Combined sources7
Beta strandi271 – 276Combined sources6
Beta strandi286 – 288Combined sources3
Helixi299 – 303Combined sources5
Turni307 – 311Combined sources5
Helixi314 – 324Combined sources11
Helixi328 – 330Combined sources3
Beta strandi331 – 344Combined sources14
Helixi348 – 351Combined sources4
Beta strandi357 – 359Combined sources3
Beta strandi364 – 366Combined sources3
Beta strandi369 – 371Combined sources3
Helixi372 – 378Combined sources7
Turni379 – 381Combined sources3
Beta strandi385 – 390Combined sources6
Turni391 – 394Combined sources4
Beta strandi395 – 400Combined sources6
Turni401 – 403Combined sources3
Beta strandi406 – 408Combined sources3
Helixi412 – 425Combined sources14
Beta strandi429 – 433Combined sources5
Helixi435 – 437Combined sources3
Helixi442 – 450Combined sources9
Beta strandi564 – 571Combined sources8
Beta strandi576 – 581Combined sources6
Beta strandi587 – 611Combined sources25
Beta strandi619 – 628Combined sources10
Beta strandi640 – 643Combined sources4
Beta strandi658 – 662Combined sources5
Beta strandi668 – 670Combined sources3
Beta strandi673 – 675Combined sources3
Beta strandi681 – 683Combined sources3
Turni693 – 695Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ED7NMR-A655-699[»]
1ITXX-ray1.10A33-451[»]
1K85NMR-A559-644[»]
ProteinModelPortaliP20533.
SMRiP20533.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20533.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini467 – 553Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST87
Domaini562 – 647Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST86

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni42 – 460CatalyticAdd BLAST419

Sequence similaritiesi

Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Family and domain databases

CDDicd00063. FN3. 2 hits.
Gene3Di2.10.10.20. 1 hit.
2.60.40.10. 2 hits.
3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR003610. CBM_fam5/12.
IPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR003961. FN3_dom.
IPR001223. Glyco_hydro18_cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF02839. CBM_5_12. 1 hit.
PF00041. fn3. 2 hits.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTiSM00495. ChtBD3. 1 hit.
SM00060. FN3. 2 hits.
SM00636. Glyco_18. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF51055. SSF51055. 1 hit.
SSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.
PROSITEiPS01095. CHITINASE_18. 1 hit.
PS50853. FN3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20533-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINLNKHTAF KKTAKFFLGL SLLLSVIVPS FALQPATAEA ADSYKIVGYY
60 70 80 90 100
PSWAAYGRNY NVADIDPTKV THINYAFADI CWNGIHGNPD PSGPNPVTWT
110 120 130 140 150
CQNEKSQTIN VPNGTIVLGD PWIDTGKTFA GDTWDQPIAG NINQLNKLKQ
160 170 180 190 200
TNPNLKTIIS VGGWTWSNRF SDVAATAATR EVFANSAVDF LRKYNFDGVD
210 220 230 240 250
LDWEYPVSGG LDGNSKRPED KQNYTLLLSK IREKLDAAGA VDGKKYLLTI
260 270 280 290 300
ASGASATYAA NTELAKIAAI VDWINIMTYD FNGAWQKISA HNAPLNYDPA
310 320 330 340 350
ASAAGVPDAN TFNVAAGAQG HLDAGVPAAK LVLGVPFYGR GWDGCAQAGN
360 370 380 390 400
GQYQTCTGGS SVGTWEAGSF DFYDLEANYI NKNGYTRYWN DTAKVPYLYN
410 420 430 440 450
ASNKRFISYD DAESVGYKTA YIKSKGLGGA MFWELSGDRN KTLQNKLKAD
460 470 480 490 500
LPTGGTVPPV DTTAPSVPGN ARSTGVTANS VTLAWNASTD NVGVTGYNVY
510 520 530 540 550
NGANLATSVT GTTATISGLT AGTSYTFTIK AKDAAGNLSA ASNAVTVSTT
560 570 580 590 600
AQPGGDTQAP TAPTNLASTA QTTSSITLSW TASTDNVGVT GYDVYNGTAL
610 620 630 640 650
ATTVTGTTAT ISGLAADTSY TFTVKAKDAA GNVSAASNAV SVKTAAETTN
660 670 680 690
PGVSAWQVNT AYTAGQLVTY NGKTYKCLQP HTSLAGWEPS NVPALWQLQ
Length:699
Mass (Da):73,678
Last modified:February 1, 1991 - v1
Checksum:iAC7C9B22E2987643
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57601 Genomic DNA. Translation: AAA81528.1.
PIRiA38368.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57601 Genomic DNA. Translation: AAA81528.1.
PIRiA38368.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ED7NMR-A655-699[»]
1ITXX-ray1.10A33-451[»]
1K85NMR-A559-644[»]
ProteinModelPortaliP20533.
SMRiP20533.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM12. Carbohydrate-Binding Module Family 12.
GH18. Glycoside Hydrolase Family 18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP20533.

Family and domain databases

CDDicd00063. FN3. 2 hits.
Gene3Di2.10.10.20. 1 hit.
2.60.40.10. 2 hits.
3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR003610. CBM_fam5/12.
IPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR003961. FN3_dom.
IPR001223. Glyco_hydro18_cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF02839. CBM_5_12. 1 hit.
PF00041. fn3. 2 hits.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTiSM00495. ChtBD3. 1 hit.
SM00060. FN3. 2 hits.
SM00636. Glyco_18. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF51055. SSF51055. 1 hit.
SSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.
PROSITEiPS01095. CHITINASE_18. 1 hit.
PS50853. FN3. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHIA1_BACCI
AccessioniPrimary (citable) accession number: P20533
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 30, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.