Reviewed, UniProtKB/Swiss-Prot P20502 (NTP2_VACCC)

Names and origin

Protein names

Recommended name:
    Nucleoside triphosphatase II
      Short name=NTPase II
    EC=3.6.1.15
Alternative name(s):
    Nucleoside triphosphate phosphohydrolase II
      Short name=NPH II
    RNA helicase I8

Gene names

Name:	NPH2
ORF Names:	I8R

Organism

Vaccinia virus (strain Copenhagen) (VACV) [Complete proteome]

Taxonomic identifier

10249 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	676 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Essential for viral replication. Plays an important role during transcription of early mRNAs, presumably by preventing R-loop formation behind the elongating RNA polymerase. Acts as NTP-dependent helicase that catalyzes unidirectional unwinding of 3'tailed duplex RNAs. Might also play a role in the export of newly synthesized mRNA chains out of the core into the cytoplasm. Required for propagation of viral particles.
Catalytic activity	NTP + H2O = NDP + phosphate.
Sequence similarities	Belongs to the DEAD box helicase family. DEAH subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Transcription
Developmental stage	Early protein Late protein
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 676	676	Nucleoside triphosphatase II		PRO_0000055183
Regions
	Domain	172 – 347	176	Helicase ATP-binding
	Domain	366 – 535	170	Helicase C-terminal
	Nucleotide binding	185 – 192	8	ATP By similarity
	Motif	296 – 299	4	DEXH box

Sequences

	Sequence		Length	Mass (Da)
	P20502-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 54ED96C44309D4F1	FASTA	676	77,659
	10 20 30 40 50 60 MEKNLPDIFF FPNCVNVFSY KYSQDEFSNM SKTERDSFSL AVFPVIKHRW HNAHVVKHKG 70 80 90 100 110 120 IYKVSTEARG KKVSPPSLGK PAHINLTTKQ YIYSEHTISF ECYSFLKCIT NTEINSFDEY 130 140 150 160 170 180 ILRGLLEAGN SLQIFSNSVG KRTDTIGVLG NKYPFSKIPL ASLTPKAQRE IFSAWISHRP 190 200 210 220 230 240 VVLTGGTGVG KTSQVPKLLL WFNYLFGGFS TLDKITDFHE RPVILSLPRI ALVRLHSNTI 250 260 270 280 290 300 LKSLGFKVLD GSPISLRYGS IPEELINKQP KKYGIVFSTH KLSLTKLFSY GTLIIDEVHE 310 320 330 340 350 360 HDQIGDIIIA VARKHHTKID SMFLMTATLE DDRERLKVFL PNPAFIHIPG DTLFKISEVF 370 380 390 400 410 420 IHNKINPSSR MAYIEEEKRN LVTAIQMYTP PDGSSGIVFV ASVAQCHEYK SYLEKRLPYD 430 440 450 460 470 480 MYIIHGKVLD IDEILEKVYS SPNVSIIIST PYLESSVTIR NVTHIYDMGR VFVPAPFGGS 490 500 510 520 530 540 QEFISKSMRD QRKGRVGRVN PGTYVYFYDL SYMKSIQRID SEFLHNYILY ANKFNLTLPE 550 560 570 580 590 600 DLFIIPTNLD ILWRTKEYID SFDISTETWN KLLSNYYMKM IEYAKLYVLS PILAEELDNF 610 620 630 640 650 660 ERTGELTSIV REAILSLNLR IKILNFKHKD DDTYIHFCKI LFGVYNGTNA TIYYHRPLTG 670 YMNMISDTIF VPVDNN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete DNA sequence of vaccinia virus." Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E. Virology 179:247-266(1990) [PubMed: 2219722] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Appendix to 'The complete DNA sequence of vaccinia virus'." Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E. Virology 179:517-563(1990) Cited for: COMPLETE GENOME.
[3]	"Vaccinia virus encodes four putative DNA and/or RNA helicases distantly related to each other." Koonin E.V., Senkevich T.G. J. Gen. Virol. 73:989-993(1992) [PubMed: 1321883] [Abstract] Cited for: SIMILARITY TO HELICASES.

Web resources

The DExH/D protein family database

Poxviridae DExH proteins

Cross-references

Sequence databases
EMBL GenBank DDBJ	M35027 Genomic DNA. Translation: AAA48064.1.
PIR	D42511.
3D structure databases
SMR	P20502. Positions 144-500.
ModBase	Search...
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR014001. DEAD-like_N. IPR001650. DNA/RNA_helicase_C. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR002464. DNA/RNA_helicase_DEAH_CS. IPR014021. Helicase_SF1/SF2_ATP-bd. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00690. DEAH_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NTP2_VACCC

Accession

Primary (citable) accession number: P20502

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	January 19, 2010
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)