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P20493 (RIR2_VACCC) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonucleoside-diphosphate reductase small chain

EC=1.17.4.1
Alternative name(s):
Ribonucleotide reductase small subunit
Gene names
ORF Names:F4L
OrganismVaccinia virus (strain Copenhagen) (VACV) [Complete proteome]
Taxonomic identifier10249 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactor

Binds 2 iron ions per subunit By similarity.

Pathway

Genetic information processing; DNA replication.

Subunit structure

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.

Induction

Expressed early in the viral replicative cycle.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase small chain family.

Ontologies

Keywords
   Biological processDNA replication
   Developmental stageEarly protein
   LigandIron
Metal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-UniPathway

deoxyribonucleoside diphosphate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 319319Ribonucleoside-diphosphate reductase small chain
PRO_0000190496

Sites

Active site1081 By similarity
Metal binding701Iron 1 By similarity
Metal binding1011Iron 1 By similarity
Metal binding1011Iron 2 By similarity
Metal binding1041Iron 1 By similarity
Metal binding1631Iron 2 By similarity
Metal binding1971Iron 2 By similarity
Metal binding2001Iron 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
P20493 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: F8F2089D0BF1ED18

FASTA31936,999
        10         20         30         40         50         60 
MEPILAPNPN RFVIFPIQYY DIWNMYKKAE ASFWTVEEVD ISKDINDWNK LTPDEKYFIK 

        70         80         90        100        110        120 
HVLAFFAASD GIVNENLAER FCTEVQITEA RCFYGFQMAI ENIHSEMYSL LIDTYVKDSN 

       130        140        150        160        170        180 
EKNYLFNAIE TMPCVKKKAD WAQKWIHDSA GYGERLIAFA AVEGIFFSGS FASIFWLKKR 

       190        200        210        220        230        240 
GLMPGLTFSN ELISRDEGLH CDFACLMFKH LLYPPSEETV RSIITDAVSI EQEFLTAALP 

       250        260        270        280        290        300 
VKLIGMNCEM MKTYIEFVAD RLISELGFKK IYNVTNPFDF MENISLEGKT NFFEKRVGEY 

       310 
QKMGVMSQED NHFSLDVDF 

« Hide

References

« Hide 'large scale' references
[1]"The complete DNA sequence of vaccinia virus."
Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E.
Virology 179:247-266(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Appendix to 'The complete DNA sequence of vaccinia virus'."
Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E.
Virology 179:517-563(1990)
Cited for: COMPLETE GENOME.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M35027 Genomic DNA. Translation: AAA48018.1.

3D structure databases

ProteinModelPortalP20493.
SMRP20493. Positions 2-282.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00326.

Family and domain databases

Gene3D1.10.620.20. 1 hit.
InterProIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERPTHR23409. PTHR23409. 1 hit.
PfamPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMSSF47240. SSF47240. 1 hit.
PROSITEPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRIR2_VACCC
AccessionPrimary (citable) accession number: P20493
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: December 11, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways