ID   CKS1_YEAST              Reviewed;         150 AA.
AC   P20486; D6VQD1;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   27-MAR-2024, entry version 203.
DE   RecName: Full=Cyclin-dependent kinases regulatory subunit;
DE   AltName: Full=Cell division control protein CKS1;
GN   Name=CKS1 {ECO:0000303|PubMed:2664468};
GN   OrderedLocusNames=YBR135W {ECO:0000312|SGD:S000000339};
GN   ORFNames=YBR1011;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBUNIT.
RX   PubMed=2664468; DOI=10.1128/mcb.9.5.2034-2041.1989;
RA   Hadwiger J.A., Wittenberg C., Mendenhall M.D., Reed S.I.;
RT   "The Saccharomyces cerevisiae CKS1 gene, a homolog of the
RT   Schizosaccharomyces pombe suc1+ gene, encodes a subunit of the Cdc28
RT   protein kinase complex.";
RL   Mol. Cell. Biol. 9:2034-2041(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8091856; DOI=10.1002/yea.320100002;
RA   Becam A.-M., Cullin C., Grzybowska E., Lacroute F., Nasr F.,
RA   Ozier-Kalogeropoulos O., Palucha A., Slonimski P.P., Zagulski M.,
RA   Herbert C.J.;
RT   "The sequence of 29.7 kb from the right arm of chromosome II reveals 13
RT   complete open reading frames, of which ten correspond to new genes.";
RL   Yeast 10:S1-S11(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA   Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA   Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA   Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA   Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA   Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA   Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA   Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA   Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA   Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA   Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA   Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA   Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA   Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA   Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA   Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA   Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA   Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA   Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA   Mewes H.-W., Kleine K.;
RT   "Complete DNA sequence of yeast chromosome II.";
RL   EMBO J. 13:5795-5809(1994).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 32-150.
RX   PubMed=8065923; DOI=10.1093/nar/22.15.3104;
RA   Kato R., Ogawa H.;
RT   "An essential gene, ESR1, is required for mitotic cell growth, DNA repair
RT   and meiotic recombination in Saccharomyces cerevisiae.";
RL   Nucleic Acids Res. 22:3104-3112(1994).
RN   [7]
RP   CHARACTERIZATION.
RX   PubMed=8491379; DOI=10.1101/gad.7.5.822;
RA   Tang Y., Reed S.I.;
RT   "The Cdk-associated protein Cks1 functions both in G1 and G2 in
RT   Saccharomyces cerevisiae.";
RL   Genes Dev. 7:822-832(1993).
RN   [8]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [9]
RP   X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
RX   PubMed=10997903; DOI=10.1016/s0969-2126(00)00175-1;
RA   Bourne Y., Watson M.H., Arvai A.S., Bernstein S.L., Reed S.I., Tainer J.A.;
RT   "Crystal structure and mutational analysis of the Saccharomyces cerevisiae
RT   cell cycle regulatory protein Cks1: implications for domain swapping, anion
RT   binding and protein interactions.";
RL   Structure 8:841-850(2000).
CC   -!- FUNCTION: Binds to the catalytic subunit of the cyclin dependent kinase
CC       (CDC28) and is essential for its biological function.
CC       {ECO:0000269|PubMed:2664468}.
CC   -!- SUBUNIT: Forms a stable but non-covalent complex with the CDC28 protein
CC       and with a cyclin. {ECO:0000269|PubMed:2664468}.
CC   -!- INTERACTION:
CC       P20486; P00546: CDC28; NbExp=9; IntAct=EBI-4746, EBI-4253;
CC       P20486; P24868: CLB1; NbExp=3; IntAct=EBI-4746, EBI-4508;
CC       P20486; P24870: CLB3; NbExp=4; IntAct=EBI-4746, EBI-4522;
CC   -!- MISCELLANEOUS: Present with 8780 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the CKS family. {ECO:0000305}.
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DR   EMBL; M26033; AAA34879.1; -; Genomic_DNA.
DR   EMBL; X75891; CAA53493.1; -; Genomic_DNA.
DR   EMBL; Z36004; CAA85092.1; -; Genomic_DNA.
DR   EMBL; AY558186; AAS56512.1; -; Genomic_DNA.
DR   EMBL; D11088; BAA01859.1; -; Genomic_DNA.
DR   EMBL; BK006936; DAA07251.1; -; Genomic_DNA.
DR   PIR; A32793; OKBYS1.
DR   RefSeq; NP_009693.3; NM_001178483.3.
DR   PDB; 1QB3; X-ray; 3.00 A; A/B/C=1-150.
DR   PDB; 3QY2; X-ray; 2.59 A; A/B=1-117.
DR   PDB; 4LPA; X-ray; 2.90 A; A/B/C/D=1-113.
DR   PDBsum; 1QB3; -.
DR   PDBsum; 3QY2; -.
DR   PDBsum; 4LPA; -.
DR   AlphaFoldDB; P20486; -.
DR   SMR; P20486; -.
DR   BioGRID; 32835; 645.
DR   ComplexPortal; CPX-1699; CLN1-CDC28 kinase complex.
DR   ComplexPortal; CPX-1700; CLN3-CDC28 kinase complex.
DR   ComplexPortal; CPX-342; CLN2-CDC28 kinase complex.
DR   DIP; DIP-1262N; -.
DR   ELM; P20486; -.
DR   IntAct; P20486; 26.
DR   MINT; P20486; -.
DR   STRING; 4932.YBR135W; -.
DR   PaxDb; 4932-YBR135W; -.
DR   PeptideAtlas; P20486; -.
DR   TopDownProteomics; P20486; -.
DR   EnsemblFungi; YBR135W_mRNA; YBR135W; YBR135W.
DR   GeneID; 852432; -.
DR   KEGG; sce:YBR135W; -.
DR   AGR; SGD:S000000339; -.
DR   SGD; S000000339; CKS1.
DR   VEuPathDB; FungiDB:YBR135W; -.
DR   eggNOG; KOG3484; Eukaryota.
DR   GeneTree; ENSGT00950000182971; -.
DR   HOGENOM; CLU_140546_1_0_1; -.
DR   InParanoid; P20486; -.
DR   OMA; RPINYGQ; -.
DR   OrthoDB; 204504at2759; -.
DR   BioCyc; YEAST:G3O-29089-MONOMER; -.
DR   BioGRID-ORCS; 852432; 6 hits in 10 CRISPR screens.
DR   EvolutionaryTrace; P20486; -.
DR   PRO; PR:P20486; -.
DR   Proteomes; UP000002311; Chromosome II.
DR   RNAct; P20486; Protein.
DR   GO; GO:0000307; C:cyclin-dependent protein kinase holoenzyme complex; IDA:SGD.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0005634; C:nucleus; HDA:SGD.
DR   GO; GO:0019005; C:SCF ubiquitin ligase complex; IBA:GO_Central.
DR   GO; GO:0061575; F:cyclin-dependent protein serine/threonine kinase activator activity; IBA:GO_Central.
DR   GO; GO:0042393; F:histone binding; IDA:SGD.
DR   GO; GO:0030295; F:protein kinase activator activity; IDA:SGD.
DR   GO; GO:0019901; F:protein kinase binding; IBA:GO_Central.
DR   GO; GO:0043130; F:ubiquitin binding; IDA:SGD.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:SGD.
DR   GO; GO:1902806; P:regulation of cell cycle G1/S phase transition; NAS:ComplexPortal.
DR   GO; GO:0007346; P:regulation of mitotic cell cycle; IMP:SGD.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:SGD.
DR   Gene3D; 3.30.170.10; Cyclin-dependent kinase, regulatory subunit; 1.
DR   InterPro; IPR000789; Cyclin-dep_kinase_reg-sub.
DR   InterPro; IPR036858; Cyclin-dep_kinase_reg-sub_sf.
DR   PANTHER; PTHR23415:SF48; CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT; 1.
DR   PANTHER; PTHR23415; CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7; 1.
DR   Pfam; PF01111; CKS; 1.
DR   PRINTS; PR00296; CYCLINKINASE.
DR   SMART; SM01084; CKS; 1.
DR   SUPFAM; SSF55637; Cell cycle regulatory proteins; 1.
DR   PROSITE; PS00944; CKS_1; 1.
DR   PROSITE; PS00945; CKS_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell cycle; Cell division; Reference proteome.
FT   CHAIN           1..150
FT                   /note="Cyclin-dependent kinases regulatory subunit"
FT                   /id="PRO_0000206245"
FT   REGION          115..150
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   HELIX           15..22
FT                   /evidence="ECO:0007829|PDB:3QY2"
FT   HELIX           23..27
FT                   /evidence="ECO:0007829|PDB:3QY2"
FT   STRAND          37..45
FT                   /evidence="ECO:0007829|PDB:3QY2"
FT   HELIX           48..53
FT                   /evidence="ECO:0007829|PDB:3QY2"
FT   HELIX           56..58
FT                   /evidence="ECO:0007829|PDB:3QY2"
FT   TURN            61..63
FT                   /evidence="ECO:0007829|PDB:3QY2"
FT   STRAND          64..66
FT                   /evidence="ECO:0007829|PDB:3QY2"
FT   HELIX           71..76
FT                   /evidence="ECO:0007829|PDB:3QY2"
FT   STRAND          86..88
FT                   /evidence="ECO:0007829|PDB:3QY2"
FT   STRAND          97..103
FT                   /evidence="ECO:0007829|PDB:3QY2"
FT   TURN            106..109
FT                   /evidence="ECO:0007829|PDB:3QY2"
SQ   SEQUENCE   150 AA;  17795 MW;  0DD0910E01D373FF CRC64;
     MYHHYHAFQG RKLTDQERAR VLEFQDSIHY SPRYSDDNYE YRHVMLPKAM LKVIPSDYFN
     SEVGTLRILT EDEWRGLGIT QSLGWEHYEC HAPEPHILLF KRPLNYEAEL RAATAAAQQQ
     QQQQQQQQQQ QQQHQTQSIS NDMQVPPQIS
//