Cyclin-dependent kinases regulatory subunit - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P20486 (CKS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 127. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinases regulatory subunit

Alternative name(s):
Cell division control protein CKS1

Gene names

Name:	CKS1
Ordered Locus Names:	YBR135W
ORF Names:	YBR1011

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	150 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to the catalytic subunit of the cyclin dependent kinase (CDC28) and is essential for its biological function.
Miscellaneous	Present with 8780 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the CKS family.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW chromatin remodeling Inferred from mutant phenotype PubMed 20855529. Source: SGD positive regulation of transcription, DNA-dependent Inferred from mutant phenotype PubMed 20516216. Source: SGD transcription, DNA-dependent Inferred from mutant phenotype PubMed 15629725. Source: SGD
Cellular_component	cyclin-dependent protein kinase holoenzyme complex Inferred from direct assay Ref.1. Source: SGD cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD nucleus Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	cyclin-dependent protein serine/threonine kinase regulator activity Inferred from electronic annotation. Source: InterPro histone binding Inferred from direct assay PubMed 20855529. Source: SGD protein kinase activator activity Inferred from direct assay PubMed 10913169. Source: SGD ubiquitin binding Inferred from direct assay PubMed 17198374. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
CDC28	P00546	8	EBI-4746,EBI-4253
CLB1	P24868	3	EBI-4746,EBI-4508
CLB3	P24870	5	EBI-4746,EBI-4522

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 150

150

Cyclin-dependent kinases regulatory subunit

PRO_0000206245

Regions

Compositional bias

118 – 133

Poly-Gln

Secondary structure

150

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P20486 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 0DD0910E01D373FF
Show »

FASTA

150

17,795

        10         20         30         40         50         60 
MYHHYHAFQG RKLTDQERAR VLEFQDSIHY SPRYSDDNYE YRHVMLPKAM LKVIPSDYFN 

        70         80         90        100        110        120 
SEVGTLRILT EDEWRGLGIT QSLGWEHYEC HAPEPHILLF KRPLNYEAEL RAATAAAQQQ 

       130        140        150 
QQQQQQQQQQ QQQHQTQSIS NDMQVPPQIS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Saccharomyces cerevisiae CKS1 gene, a homolog of the Schizosaccharomyces pombe suc1+ gene, encodes a subunit of the Cdc28 protein kinase complex." Hadwiger J.A., Wittenberg C., Mendenhall M.D., Reed S.I. Mol. Cell. Biol. 9:2034-2041(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The sequence of 29.7 kb from the right arm of chromosome II reveals 13 complete open reading frames, of which ten correspond to new genes." Becam A.-M., Cullin C., Grzybowska E., Lacroute F., Nasr F., Ozier-Kalogeropoulos O., Palucha A., Slonimski P.P., Zagulski M., Herbert C.J. Yeast 10:S1-S11(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"An essential gene, ESR1, is required for mitotic cell growth, DNA repair and meiotic recombination in Saccharomyces cerevisiae." Kato R., Ogawa H. Nucleic Acids Res. 22:3104-3112(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 32-150.
[7]	"The Cdk-associated protein Cks1 functions both in G1 and G2 in Saccharomyces cerevisiae." Tang Y., Reed S.I. Genes Dev. 7:822-832(1993) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Crystal structure and mutational analysis of the Saccharomyces cerevisiae cell cycle regulatory protein Cks1: implications for domain swapping, anion binding and protein interactions." Bourne Y., Watson M.H., Arvai A.S., Bernstein S.L., Reed S.I., Tainer J.A. Structure 8:841-850(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M26033 Genomic DNA. Translation: AAA34879.1.
X75891 Genomic DNA. Translation: CAA53493.1.
Z36004 Genomic DNA. Translation: CAA85092.1.
AY558186 Genomic DNA. Translation: AAS56512.1.
D11088 Genomic DNA. Translation: BAA01859.1.
BK006936 Genomic DNA. Translation: DAA07251.1.

PIR

OKBYS1. A32793.

RefSeq

NP_009693.3. NM_001178483.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1QB3	X-ray	3.00	A/B/C	1-150	[»]
3QY2	X-ray	2.59	A/B	1-117	[»]

ProteinModelPortal

P20486.

SMR

P20486. Positions 6-114.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1262N.

IntAct

P20486. 23 interactions.

MINT

MINT-410972.

STRING

4932.YBR135W.

Proteomic databases

PaxDb

P20486.

PeptideAtlas

P20486.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YBR135W; YBR135W; YBR135W.

GeneID

852432.

KEGG

sce:YBR135W.
sce:YBR139W.

Organism-specific databases

CYGD

YBR135w.

SGD

S000000339. CKS1.

Phylogenomic databases

eggNOG

NOG272189.

GeneTree

ENSGT00390000011006.

HOGENOM

HOG000157009.

K02219.
K13289.

OMA

HYSPRYS.

OrthoDB

EOG4MWCGP.

Gene expression databases

Genevestigator

P20486.

GermOnline

YBR135W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

3.30.170.10. 1 hit.

InterPro

IPR000789. Cyclin-dep_kinase_reg-sub.
[Graphical view]

Pfam

PF01111. CKS. 1 hit.
[Graphical view]

PRINTS

PR00296. CYCLINKINASE.

SMART

SM01084. CKS. 1 hit.
[Graphical view]

SUPFAM

SSF55637. Cyc_dep_kin_rsub. 1 hit.

PROSITE

PS00944. CKS_1. 1 hit.
PS00945. CKS_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P20486.

NextBio

971315.

Entry information

Entry name

CKS1_YEAST

Accession

Primary (citable) accession number: P20486
Secondary accession number(s): D6VQD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	May 1, 2013
This is version 127 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents