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P20486

- CKS1_YEAST

UniProt

P20486 - CKS1_YEAST

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Protein

Cyclin-dependent kinases regulatory subunit

Gene

CKS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Binds to the catalytic subunit of the cyclin dependent kinase (CDC28) and is essential for its biological function.

GO - Molecular functioni

  1. cyclin-dependent protein serine/threonine kinase regulator activity Source: InterPro
  2. histone binding Source: SGD
  3. protein kinase activator activity Source: SGD
  4. ubiquitin binding Source: SGD

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. chromatin remodeling Source: SGD
  4. positive regulation of protein kinase activity Source: GOC
  5. positive regulation of transcription, DNA-templated Source: SGD
  6. regulation of mitotic cell cycle Source: SGD
  7. transcription, DNA-templated Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Enzyme and pathway databases

BioCyciYEAST:G3O-29089-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinases regulatory subunit
Alternative name(s):
Cell division control protein CKS1
Gene namesi
Name:CKS1
Ordered Locus Names:YBR135W
ORF Names:YBR1011
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR135w.
SGDiS000000339. CKS1.

Subcellular locationi

GO - Cellular componenti

  1. cyclin-dependent protein kinase holoenzyme complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 150150Cyclin-dependent kinases regulatory subunitPRO_0000206245Add
BLAST

Proteomic databases

PaxDbiP20486.
PeptideAtlasiP20486.

Expressioni

Gene expression databases

GenevestigatoriP20486.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC28P005467EBI-4746,EBI-4253
CLB1P248683EBI-4746,EBI-4508
CLB3P248704EBI-4746,EBI-4522

Protein-protein interaction databases

BioGridi32835. 181 interactions.
DIPiDIP-1262N.
IntActiP20486. 18 interactions.
MINTiMINT-410972.
STRINGi4932.YBR135W.

Structurei

Secondary structure

1
150
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi15 – 228Combined sources
Helixi23 – 275Combined sources
Beta strandi37 – 459Combined sources
Helixi48 – 536Combined sources
Helixi56 – 583Combined sources
Turni61 – 633Combined sources
Beta strandi64 – 663Combined sources
Helixi71 – 766Combined sources
Beta strandi86 – 883Combined sources
Beta strandi97 – 1037Combined sources
Helixi106 – 11611Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QB3X-ray3.00A/B/C1-150[»]
3QY2X-ray2.59A/B1-117[»]
4LPAX-ray2.90A/B/C/D1-113[»]
ProteinModelPortaliP20486.
SMRiP20486. Positions 7-113.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20486.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi118 – 13316Poly-GlnAdd
BLAST

Sequence similaritiesi

Belongs to the CKS family.Curated

Phylogenomic databases

eggNOGiNOG272189.
GeneTreeiENSGT00390000011006.
HOGENOMiHOG000157009.
InParanoidiP20486.
KOiK02219.
OMAiDYFNSEV.
OrthoDBiEOG78D7Z8.

Family and domain databases

Gene3Di3.30.170.10. 1 hit.
InterProiIPR000789. Cyclin-dep_kinase_reg-sub.
[Graphical view]
PfamiPF01111. CKS. 1 hit.
[Graphical view]
PRINTSiPR00296. CYCLINKINASE.
SMARTiSM01084. CKS. 1 hit.
[Graphical view]
SUPFAMiSSF55637. SSF55637. 1 hit.
PROSITEiPS00944. CKS_1. 1 hit.
PS00945. CKS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20486-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYHHYHAFQG RKLTDQERAR VLEFQDSIHY SPRYSDDNYE YRHVMLPKAM
60 70 80 90 100
LKVIPSDYFN SEVGTLRILT EDEWRGLGIT QSLGWEHYEC HAPEPHILLF
110 120 130 140 150
KRPLNYEAEL RAATAAAQQQ QQQQQQQQQQ QQQHQTQSIS NDMQVPPQIS
Length:150
Mass (Da):17,795
Last modified:February 1, 1991 - v1
Checksum:i0DD0910E01D373FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26033 Genomic DNA. Translation: AAA34879.1.
X75891 Genomic DNA. Translation: CAA53493.1.
Z36004 Genomic DNA. Translation: CAA85092.1.
AY558186 Genomic DNA. Translation: AAS56512.1.
D11088 Genomic DNA. Translation: BAA01859.1.
BK006936 Genomic DNA. Translation: DAA07251.1.
PIRiA32793. OKBYS1.
RefSeqiNP_009693.3. NM_001178483.3.

Genome annotation databases

EnsemblFungiiYBR135W; YBR135W; YBR135W.
GeneIDi852432.
KEGGisce:YBR135W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26033 Genomic DNA. Translation: AAA34879.1 .
X75891 Genomic DNA. Translation: CAA53493.1 .
Z36004 Genomic DNA. Translation: CAA85092.1 .
AY558186 Genomic DNA. Translation: AAS56512.1 .
D11088 Genomic DNA. Translation: BAA01859.1 .
BK006936 Genomic DNA. Translation: DAA07251.1 .
PIRi A32793. OKBYS1.
RefSeqi NP_009693.3. NM_001178483.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1QB3 X-ray 3.00 A/B/C 1-150 [» ]
3QY2 X-ray 2.59 A/B 1-117 [» ]
4LPA X-ray 2.90 A/B/C/D 1-113 [» ]
ProteinModelPortali P20486.
SMRi P20486. Positions 7-113.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32835. 181 interactions.
DIPi DIP-1262N.
IntActi P20486. 18 interactions.
MINTi MINT-410972.
STRINGi 4932.YBR135W.

Proteomic databases

PaxDbi P20486.
PeptideAtlasi P20486.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR135W ; YBR135W ; YBR135W .
GeneIDi 852432.
KEGGi sce:YBR135W.

Organism-specific databases

CYGDi YBR135w.
SGDi S000000339. CKS1.

Phylogenomic databases

eggNOGi NOG272189.
GeneTreei ENSGT00390000011006.
HOGENOMi HOG000157009.
InParanoidi P20486.
KOi K02219.
OMAi DYFNSEV.
OrthoDBi EOG78D7Z8.

Enzyme and pathway databases

BioCyci YEAST:G3O-29089-MONOMER.

Miscellaneous databases

EvolutionaryTracei P20486.
NextBioi 971315.

Gene expression databases

Genevestigatori P20486.

Family and domain databases

Gene3Di 3.30.170.10. 1 hit.
InterProi IPR000789. Cyclin-dep_kinase_reg-sub.
[Graphical view ]
Pfami PF01111. CKS. 1 hit.
[Graphical view ]
PRINTSi PR00296. CYCLINKINASE.
SMARTi SM01084. CKS. 1 hit.
[Graphical view ]
SUPFAMi SSF55637. SSF55637. 1 hit.
PROSITEi PS00944. CKS_1. 1 hit.
PS00945. CKS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Saccharomyces cerevisiae CKS1 gene, a homolog of the Schizosaccharomyces pombe suc1+ gene, encodes a subunit of the Cdc28 protein kinase complex."
    Hadwiger J.A., Wittenberg C., Mendenhall M.D., Reed S.I.
    Mol. Cell. Biol. 9:2034-2041(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The sequence of 29.7 kb from the right arm of chromosome II reveals 13 complete open reading frames, of which ten correspond to new genes."
    Becam A.-M., Cullin C., Grzybowska E., Lacroute F., Nasr F., Ozier-Kalogeropoulos O., Palucha A., Slonimski P.P., Zagulski M., Herbert C.J.
    Yeast 10:S1-S11(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "An essential gene, ESR1, is required for mitotic cell growth, DNA repair and meiotic recombination in Saccharomyces cerevisiae."
    Kato R., Ogawa H.
    Nucleic Acids Res. 22:3104-3112(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 32-150.
  7. "The Cdk-associated protein Cks1 functions both in G1 and G2 in Saccharomyces cerevisiae."
    Tang Y., Reed S.I.
    Genes Dev. 7:822-832(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Crystal structure and mutational analysis of the Saccharomyces cerevisiae cell cycle regulatory protein Cks1: implications for domain swapping, anion binding and protein interactions."
    Bourne Y., Watson M.H., Arvai A.S., Bernstein S.L., Reed S.I., Tainer J.A.
    Structure 8:841-850(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).

Entry informationi

Entry nameiCKS1_YEAST
AccessioniPrimary (citable) accession number: P20486
Secondary accession number(s): D6VQD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 26, 2014
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8780 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3