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P20486

- CKS1_YEAST

UniProt

P20486 - CKS1_YEAST

Protein

Cyclin-dependent kinases regulatory subunit

Gene

CKS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Binds to the catalytic subunit of the cyclin dependent kinase (CDC28) and is essential for its biological function.

    GO - Molecular functioni

    1. cyclin-dependent protein serine/threonine kinase regulator activity Source: InterPro
    2. histone binding Source: SGD
    3. protein binding Source: IntAct
    4. protein kinase activator activity Source: SGD
    5. ubiquitin binding Source: SGD

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. chromatin remodeling Source: SGD
    4. positive regulation of protein kinase activity Source: GOC
    5. positive regulation of transcription, DNA-templated Source: SGD
    6. regulation of mitotic cell cycle Source: SGD
    7. transcription, DNA-templated Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29089-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinases regulatory subunit
    Alternative name(s):
    Cell division control protein CKS1
    Gene namesi
    Name:CKS1
    Ordered Locus Names:YBR135W
    ORF Names:YBR1011
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR135w.
    SGDiS000000339. CKS1.

    Subcellular locationi

    GO - Cellular componenti

    1. cyclin-dependent protein kinase holoenzyme complex Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 150150Cyclin-dependent kinases regulatory subunitPRO_0000206245Add
    BLAST

    Proteomic databases

    PaxDbiP20486.
    PeptideAtlasiP20486.

    Expressioni

    Gene expression databases

    GenevestigatoriP20486.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CDC28P005467EBI-4746,EBI-4253
    CLB1P248683EBI-4746,EBI-4508
    CLB3P248704EBI-4746,EBI-4522

    Protein-protein interaction databases

    BioGridi32835. 181 interactions.
    DIPiDIP-1262N.
    IntActiP20486. 18 interactions.
    MINTiMINT-410972.
    STRINGi4932.YBR135W.

    Structurei

    Secondary structure

    1
    150
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi15 – 228
    Helixi23 – 275
    Beta strandi37 – 459
    Helixi48 – 536
    Helixi56 – 583
    Turni61 – 633
    Beta strandi64 – 663
    Helixi71 – 766
    Beta strandi86 – 883
    Beta strandi97 – 1037
    Helixi106 – 11611

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1QB3X-ray3.00A/B/C1-150[»]
    3QY2X-ray2.59A/B1-117[»]
    4LPAX-ray2.90A/B/C/D1-113[»]
    ProteinModelPortaliP20486.
    SMRiP20486. Positions 7-113.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP20486.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi118 – 13316Poly-GlnAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CKS family.Curated

    Phylogenomic databases

    eggNOGiNOG272189.
    GeneTreeiENSGT00390000011006.
    HOGENOMiHOG000157009.
    KOiK02219.
    OMAiDYFNSEV.
    OrthoDBiEOG78D7Z8.

    Family and domain databases

    Gene3Di3.30.170.10. 1 hit.
    InterProiIPR000789. Cyclin-dep_kinase_reg-sub.
    [Graphical view]
    PfamiPF01111. CKS. 1 hit.
    [Graphical view]
    PRINTSiPR00296. CYCLINKINASE.
    SMARTiSM01084. CKS. 1 hit.
    [Graphical view]
    SUPFAMiSSF55637. SSF55637. 1 hit.
    PROSITEiPS00944. CKS_1. 1 hit.
    PS00945. CKS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P20486-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYHHYHAFQG RKLTDQERAR VLEFQDSIHY SPRYSDDNYE YRHVMLPKAM    50
    LKVIPSDYFN SEVGTLRILT EDEWRGLGIT QSLGWEHYEC HAPEPHILLF 100
    KRPLNYEAEL RAATAAAQQQ QQQQQQQQQQ QQQHQTQSIS NDMQVPPQIS 150
    Length:150
    Mass (Da):17,795
    Last modified:February 1, 1991 - v1
    Checksum:i0DD0910E01D373FF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26033 Genomic DNA. Translation: AAA34879.1.
    X75891 Genomic DNA. Translation: CAA53493.1.
    Z36004 Genomic DNA. Translation: CAA85092.1.
    AY558186 Genomic DNA. Translation: AAS56512.1.
    D11088 Genomic DNA. Translation: BAA01859.1.
    BK006936 Genomic DNA. Translation: DAA07251.1.
    PIRiA32793. OKBYS1.
    RefSeqiNP_009693.3. NM_001178483.3.

    Genome annotation databases

    EnsemblFungiiYBR135W; YBR135W; YBR135W.
    GeneIDi852432.
    KEGGisce:YBR135W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26033 Genomic DNA. Translation: AAA34879.1 .
    X75891 Genomic DNA. Translation: CAA53493.1 .
    Z36004 Genomic DNA. Translation: CAA85092.1 .
    AY558186 Genomic DNA. Translation: AAS56512.1 .
    D11088 Genomic DNA. Translation: BAA01859.1 .
    BK006936 Genomic DNA. Translation: DAA07251.1 .
    PIRi A32793. OKBYS1.
    RefSeqi NP_009693.3. NM_001178483.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1QB3 X-ray 3.00 A/B/C 1-150 [» ]
    3QY2 X-ray 2.59 A/B 1-117 [» ]
    4LPA X-ray 2.90 A/B/C/D 1-113 [» ]
    ProteinModelPortali P20486.
    SMRi P20486. Positions 7-113.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32835. 181 interactions.
    DIPi DIP-1262N.
    IntActi P20486. 18 interactions.
    MINTi MINT-410972.
    STRINGi 4932.YBR135W.

    Proteomic databases

    PaxDbi P20486.
    PeptideAtlasi P20486.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR135W ; YBR135W ; YBR135W .
    GeneIDi 852432.
    KEGGi sce:YBR135W.

    Organism-specific databases

    CYGDi YBR135w.
    SGDi S000000339. CKS1.

    Phylogenomic databases

    eggNOGi NOG272189.
    GeneTreei ENSGT00390000011006.
    HOGENOMi HOG000157009.
    KOi K02219.
    OMAi DYFNSEV.
    OrthoDBi EOG78D7Z8.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29089-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P20486.
    NextBioi 971315.

    Gene expression databases

    Genevestigatori P20486.

    Family and domain databases

    Gene3Di 3.30.170.10. 1 hit.
    InterProi IPR000789. Cyclin-dep_kinase_reg-sub.
    [Graphical view ]
    Pfami PF01111. CKS. 1 hit.
    [Graphical view ]
    PRINTSi PR00296. CYCLINKINASE.
    SMARTi SM01084. CKS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55637. SSF55637. 1 hit.
    PROSITEi PS00944. CKS_1. 1 hit.
    PS00945. CKS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Saccharomyces cerevisiae CKS1 gene, a homolog of the Schizosaccharomyces pombe suc1+ gene, encodes a subunit of the Cdc28 protein kinase complex."
      Hadwiger J.A., Wittenberg C., Mendenhall M.D., Reed S.I.
      Mol. Cell. Biol. 9:2034-2041(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The sequence of 29.7 kb from the right arm of chromosome II reveals 13 complete open reading frames, of which ten correspond to new genes."
      Becam A.-M., Cullin C., Grzybowska E., Lacroute F., Nasr F., Ozier-Kalogeropoulos O., Palucha A., Slonimski P.P., Zagulski M., Herbert C.J.
      Yeast 10:S1-S11(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "An essential gene, ESR1, is required for mitotic cell growth, DNA repair and meiotic recombination in Saccharomyces cerevisiae."
      Kato R., Ogawa H.
      Nucleic Acids Res. 22:3104-3112(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 32-150.
    7. "The Cdk-associated protein Cks1 functions both in G1 and G2 in Saccharomyces cerevisiae."
      Tang Y., Reed S.I.
      Genes Dev. 7:822-832(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Crystal structure and mutational analysis of the Saccharomyces cerevisiae cell cycle regulatory protein Cks1: implications for domain swapping, anion binding and protein interactions."
      Bourne Y., Watson M.H., Arvai A.S., Bernstein S.L., Reed S.I., Tainer J.A.
      Structure 8:841-850(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).

    Entry informationi

    Entry nameiCKS1_YEAST
    AccessioniPrimary (citable) accession number: P20486
    Secondary accession number(s): D6VQD1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 139 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 8780 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3