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P20486 (CKS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 138. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinases regulatory subunit
Alternative name(s):
Cell division control protein CKS1
Gene names
Name:CKS1
Ordered Locus Names:YBR135W
ORF Names:YBR1011
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length150 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to the catalytic subunit of the cyclin dependent kinase (CDC28) and is essential for its biological function.

Miscellaneous

Present with 8780 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the CKS family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 150150Cyclin-dependent kinases regulatory subunit
PRO_0000206245

Regions

Compositional bias118 – 13316Poly-Gln

Secondary structure

..................... 150
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P20486 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 0DD0910E01D373FF

FASTA15017,795
        10         20         30         40         50         60 
MYHHYHAFQG RKLTDQERAR VLEFQDSIHY SPRYSDDNYE YRHVMLPKAM LKVIPSDYFN 

        70         80         90        100        110        120 
SEVGTLRILT EDEWRGLGIT QSLGWEHYEC HAPEPHILLF KRPLNYEAEL RAATAAAQQQ 

       130        140        150 
QQQQQQQQQQ QQQHQTQSIS NDMQVPPQIS 

« Hide

References

« Hide 'large scale' references
[1]"The Saccharomyces cerevisiae CKS1 gene, a homolog of the Schizosaccharomyces pombe suc1+ gene, encodes a subunit of the Cdc28 protein kinase complex."
Hadwiger J.A., Wittenberg C., Mendenhall M.D., Reed S.I.
Mol. Cell. Biol. 9:2034-2041(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The sequence of 29.7 kb from the right arm of chromosome II reveals 13 complete open reading frames, of which ten correspond to new genes."
Becam A.-M., Cullin C., Grzybowska E., Lacroute F., Nasr F., Ozier-Kalogeropoulos O., Palucha A., Slonimski P.P., Zagulski M., Herbert C.J.
Yeast 10:S1-S11(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"An essential gene, ESR1, is required for mitotic cell growth, DNA repair and meiotic recombination in Saccharomyces cerevisiae."
Kato R., Ogawa H.
Nucleic Acids Res. 22:3104-3112(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 32-150.
[7]"The Cdk-associated protein Cks1 functions both in G1 and G2 in Saccharomyces cerevisiae."
Tang Y., Reed S.I.
Genes Dev. 7:822-832(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Crystal structure and mutational analysis of the Saccharomyces cerevisiae cell cycle regulatory protein Cks1: implications for domain swapping, anion binding and protein interactions."
Bourne Y., Watson M.H., Arvai A.S., Bernstein S.L., Reed S.I., Tainer J.A.
Structure 8:841-850(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M26033 Genomic DNA. Translation: AAA34879.1.
X75891 Genomic DNA. Translation: CAA53493.1.
Z36004 Genomic DNA. Translation: CAA85092.1.
AY558186 Genomic DNA. Translation: AAS56512.1.
D11088 Genomic DNA. Translation: BAA01859.1.
BK006936 Genomic DNA. Translation: DAA07251.1.
PIROKBYS1. A32793.
RefSeqNP_009693.3. NM_001178483.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1QB3X-ray3.00A/B/C1-150[»]
3QY2X-ray2.59A/B1-117[»]
4LPAX-ray2.90A/B/C/D1-113[»]
ProteinModelPortalP20486.
SMRP20486. Positions 7-113.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32835. 181 interactions.
DIPDIP-1262N.
IntActP20486. 18 interactions.
MINTMINT-410972.
STRING4932.YBR135W.

Proteomic databases

PaxDbP20486.
PeptideAtlasP20486.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR135W; YBR135W; YBR135W.
GeneID852432.
KEGGsce:YBR135W.

Organism-specific databases

CYGDYBR135w.
SGDS000000339. CKS1.

Phylogenomic databases

eggNOGNOG272189.
GeneTreeENSGT00390000011006.
HOGENOMHOG000157009.
KOK02219.
OMADYFNSEV.
OrthoDBEOG78D7Z8.

Enzyme and pathway databases

BioCycYEAST:G3O-29089-MONOMER.

Gene expression databases

GenevestigatorP20486.

Family and domain databases

Gene3D3.30.170.10. 1 hit.
InterProIPR000789. Cyclin-dep_kinase_reg-sub.
[Graphical view]
PfamPF01111. CKS. 1 hit.
[Graphical view]
PRINTSPR00296. CYCLINKINASE.
SMARTSM01084. CKS. 1 hit.
[Graphical view]
SUPFAMSSF55637. SSF55637. 1 hit.
PROSITEPS00944. CKS_1. 1 hit.
PS00945. CKS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP20486.
NextBio971315.

Entry information

Entry nameCKS1_YEAST
AccessionPrimary (citable) accession number: P20486
Secondary accession number(s): D6VQD1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 9, 2014
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references