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P20485 (KICH_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Choline kinase

EC=2.7.1.32
Gene names
Name:CKI1
Synonyms:CKI
Ordered Locus Names:YLR133W
ORF Names:L3130, L9606.8
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length582 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Responsible for phosphatidylcholine synthesis via the CDP-choline pathway. Also exhibits ethanolamine kinase activity but at 14% efficiency compared with choline.

Catalytic activity

ATP + choline = ADP + phosphocholine.

Pathway

Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphocholine from choline: step 1/1.

Subunit structure

Monomer. Interacts with NAP1. Ref.9

Subcellular location

Cytoplasm.

Miscellaneous

Present with 3930 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the choline/ethanolamine kinase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

NAP1P252935EBI-9699,EBI-11850

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 582582Choline kinase
PRO_0000206226

Amino acid modifications

Modified residue301Phosphoserine; by PKA Ref.5
Modified residue481Phosphoserine Ref.11
Modified residue511Phosphoserine Ref.11
Modified residue541Phosphothreonine Ref.11
Modified residue851Phosphoserine; by PKA Ref.5

Experimental info

Mutagenesis301S → A: Decrease in activity and in phosphorylation by PKA. No phosphorylation by PKA, and strong decrease in activity; when associated with A-85. Ref.5
Mutagenesis851S → A: No phosphorylation by PKA, and strong decrease in activity; when associated with A-30. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P20485 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 68C395B9CC120A0E

FASTA58266,317
        10         20         30         40         50         60 
MVQESRPGSV RSYSVGYQAR SRSSSQRRHS LTRQRSSQRL IRTISIESDV SNITDDDDLR 

        70         80         90        100        110        120 
AVNEGVAGVQ LDVSETANKG PRRASATDVT DSLGSTSSEY IEIPFVKETL DASLPSDYLK 

       130        140        150        160        170        180 
QDILNLIQSL KISKWYNNKK IQPVAQDMNL VKISGAMTNA IFKVEYPKLP SLLLRIYGPN 

       190        200        210        220        230        240 
IDNIIDREYE LQILARLSLK NIGPSLYGCF VNGRFEQFLE NSKTLTKDDI RNWKNSQRIA 

       250        260        270        280        290        300 
RRMKELHVGV PLLSSERKNG SACWQKINQW LRTIEKVDQW VGDPKNIENS LLCENWSKFM 

       310        320        330        340        350        360 
DIVDRYHKWL ISQEQGIEQV NKNLIFCHND AQYGNLLFTA PVMNTPSLYT APSSTSLTSQ 

       370        380        390        400        410        420 
SSSLFPSSSN VIVDDIINPP KQEQSQDSKL VVIDFEYAGA NPAAYDLANH LSEWMYDYNN 

       430        440        450        460        470        480 
AKAPHQCHAD RYPDKEQVLN FLYSYVSHLR GGAKEPIDEE VQRLYKSIIQ WRPTVQLFWS 

       490        500        510        520        530        540 
LWAILQSGKL EKKEASTAIT REEIGPNGKK YIIKTEPESP EEDFVENDDE PEAGVSIDTF 

       550        560        570        580 
DYMAYGRDKI AVFWGDLIGL GIITEEECKN FSSFKFLDTS YL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of the yeast CKI gene encoding choline kinase and its expression in Escherichia coli."
Hosaka K., Kodaki T., Yamashita S.
J. Biol. Chem. 264:2053-2059(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Phosphorylation of Saccharomyces cerevisiae choline kinase on Ser30 and Ser85 by protein kinase A regulates phosphatidylcholine synthesis by the CDP-choline pathway."
Yu Y., Sreenivas A., Ostrander D.B., Carman G.M.
J. Biol. Chem. 277:34978-34986(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-30 AND SER-85, MUTAGENESIS OF SER-30 AND SER-85.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"Phosphorylation by casein kinase 2 regulates Nap1 localization and function."
Calvert M.E.K., Keck K.M., Ptak C., Shabanowitz J., Hunt D.F., Pemberton L.F.
Mol. Cell. Biol. 28:1313-1325(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NAP1, IDENTIFICATION BY MASS SPECTROMETRY.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-48; SER-51 AND THR-54, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J04454 Genomic DNA. Translation: AAA34499.1.
X91258 Genomic DNA. Translation: CAA62646.1.
Z73305 Genomic DNA. Translation: CAA97704.1.
U53881 Genomic DNA. Translation: AAB82396.1.
AY692779 Genomic DNA. Translation: AAT92798.1.
BK006945 Genomic DNA. Translation: DAA09444.1.
PIRA32034.
RefSeqNP_013234.1. NM_001182020.1.

3D structure databases

ProteinModelPortalP20485.
SMRP20485. Positions 139-487.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31402. 78 interactions.
DIPDIP-2617N.
IntActP20485. 6 interactions.
MINTMINT-422889.
STRING4932.YLR133W.

Proteomic databases

MaxQBP20485.
PaxDbP20485.
PeptideAtlasP20485.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR133W; YLR133W; YLR133W.
GeneID850824.
KEGGsce:YLR133W.

Organism-specific databases

CYGDYLR133w.
SGDS000004123. CKI1.

Phylogenomic databases

eggNOGCOG0510.
GeneTreeENSGT00530000062991.
HOGENOMHOG000246747.
KOK00866.
OMAKFCHNDT.
OrthoDBEOG7WT48T.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-13669.
YEAST:YLR133W-MONOMER.
UniPathwayUPA00753; UER00737.

Gene expression databases

GenevestigatorP20485.

Family and domain databases

InterProIPR026712. Cho/Etha_kinase.
IPR007521. Choline_kin_N.
IPR011009. Kinase-like_dom.
[Graphical view]
PANTHERPTHR22603:SF32. PTHR22603:SF32. 1 hit.
PfamPF04428. Choline_kin_N. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 2 hits.
ProtoNetSearch...

Other

NextBio967079.

Entry information

Entry nameKICH_YEAST
AccessionPrimary (citable) accession number: P20485
Secondary accession number(s): D6VYC8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: June 11, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways