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P20485

- KICH_YEAST

UniProt

P20485 - KICH_YEAST

Protein

Choline kinase

Gene

CKI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Responsible for phosphatidylcholine synthesis via the CDP-choline pathway. Also exhibits ethanolamine kinase activity but at 14% efficiency compared with choline.

    Catalytic activityi

    ATP + choline = ADP + phosphocholine.

    Pathwayi

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. choline kinase activity Source: SGD
    3. ethanolamine kinase activity Source: SGD
    4. protein binding Source: IntAct

    GO - Biological processi

    1. CDP-choline pathway Source: GOC
    2. phosphatidylcholine biosynthetic process Source: SGD
    3. phosphatidylethanolamine biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13669.
    YEAST:YLR133W-MONOMER.
    UniPathwayiUPA00753; UER00737.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Choline kinase (EC:2.7.1.32)
    Gene namesi
    Name:CKI1
    Synonyms:CKI
    Ordered Locus Names:YLR133W
    ORF Names:L3130, L9606.8
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR133w.
    SGDiS000004123. CKI1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi30 – 301S → A: Decrease in activity and in phosphorylation by PKA. No phosphorylation by PKA, and strong decrease in activity; when associated with A-85. 1 Publication
    Mutagenesisi85 – 851S → A: No phosphorylation by PKA, and strong decrease in activity; when associated with A-30. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 582582Choline kinasePRO_0000206226Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei30 – 301Phosphoserine; by PKA1 Publication
    Modified residuei48 – 481Phosphoserine1 Publication
    Modified residuei51 – 511Phosphoserine1 Publication
    Modified residuei54 – 541Phosphothreonine1 Publication
    Modified residuei85 – 851Phosphoserine; by PKA1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP20485.
    PaxDbiP20485.
    PeptideAtlasiP20485.

    Expressioni

    Gene expression databases

    GenevestigatoriP20485.

    Interactioni

    Subunit structurei

    Monomer. Interacts with NAP1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    NAP1P252935EBI-9699,EBI-11850

    Protein-protein interaction databases

    BioGridi31402. 78 interactions.
    DIPiDIP-2617N.
    IntActiP20485. 6 interactions.
    MINTiMINT-422889.
    STRINGi4932.YLR133W.

    Structurei

    3D structure databases

    ProteinModelPortaliP20485.
    SMRiP20485. Positions 139-487.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the choline/ethanolamine kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0510.
    GeneTreeiENSGT00530000062991.
    HOGENOMiHOG000246747.
    KOiK00866.
    OMAiKFCHNDT.
    OrthoDBiEOG7WT48T.

    Family and domain databases

    InterProiIPR026712. Cho/Etha_kinase.
    IPR007521. Choline_kin_N.
    IPR011009. Kinase-like_dom.
    [Graphical view]
    PANTHERiPTHR22603:SF32. PTHR22603:SF32. 1 hit.
    PfamiPF04428. Choline_kin_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P20485-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVQESRPGSV RSYSVGYQAR SRSSSQRRHS LTRQRSSQRL IRTISIESDV    50
    SNITDDDDLR AVNEGVAGVQ LDVSETANKG PRRASATDVT DSLGSTSSEY 100
    IEIPFVKETL DASLPSDYLK QDILNLIQSL KISKWYNNKK IQPVAQDMNL 150
    VKISGAMTNA IFKVEYPKLP SLLLRIYGPN IDNIIDREYE LQILARLSLK 200
    NIGPSLYGCF VNGRFEQFLE NSKTLTKDDI RNWKNSQRIA RRMKELHVGV 250
    PLLSSERKNG SACWQKINQW LRTIEKVDQW VGDPKNIENS LLCENWSKFM 300
    DIVDRYHKWL ISQEQGIEQV NKNLIFCHND AQYGNLLFTA PVMNTPSLYT 350
    APSSTSLTSQ SSSLFPSSSN VIVDDIINPP KQEQSQDSKL VVIDFEYAGA 400
    NPAAYDLANH LSEWMYDYNN AKAPHQCHAD RYPDKEQVLN FLYSYVSHLR 450
    GGAKEPIDEE VQRLYKSIIQ WRPTVQLFWS LWAILQSGKL EKKEASTAIT 500
    REEIGPNGKK YIIKTEPESP EEDFVENDDE PEAGVSIDTF DYMAYGRDKI 550
    AVFWGDLIGL GIITEEECKN FSSFKFLDTS YL 582
    Length:582
    Mass (Da):66,317
    Last modified:February 1, 1991 - v1
    Checksum:i68C395B9CC120A0E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04454 Genomic DNA. Translation: AAA34499.1.
    X91258 Genomic DNA. Translation: CAA62646.1.
    Z73305 Genomic DNA. Translation: CAA97704.1.
    U53881 Genomic DNA. Translation: AAB82396.1.
    AY692779 Genomic DNA. Translation: AAT92798.1.
    BK006945 Genomic DNA. Translation: DAA09444.1.
    PIRiA32034.
    RefSeqiNP_013234.1. NM_001182020.1.

    Genome annotation databases

    EnsemblFungiiYLR133W; YLR133W; YLR133W.
    GeneIDi850824.
    KEGGisce:YLR133W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04454 Genomic DNA. Translation: AAA34499.1 .
    X91258 Genomic DNA. Translation: CAA62646.1 .
    Z73305 Genomic DNA. Translation: CAA97704.1 .
    U53881 Genomic DNA. Translation: AAB82396.1 .
    AY692779 Genomic DNA. Translation: AAT92798.1 .
    BK006945 Genomic DNA. Translation: DAA09444.1 .
    PIRi A32034.
    RefSeqi NP_013234.1. NM_001182020.1.

    3D structure databases

    ProteinModelPortali P20485.
    SMRi P20485. Positions 139-487.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31402. 78 interactions.
    DIPi DIP-2617N.
    IntActi P20485. 6 interactions.
    MINTi MINT-422889.
    STRINGi 4932.YLR133W.

    Proteomic databases

    MaxQBi P20485.
    PaxDbi P20485.
    PeptideAtlasi P20485.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR133W ; YLR133W ; YLR133W .
    GeneIDi 850824.
    KEGGi sce:YLR133W.

    Organism-specific databases

    CYGDi YLR133w.
    SGDi S000004123. CKI1.

    Phylogenomic databases

    eggNOGi COG0510.
    GeneTreei ENSGT00530000062991.
    HOGENOMi HOG000246747.
    KOi K00866.
    OMAi KFCHNDT.
    OrthoDBi EOG7WT48T.

    Enzyme and pathway databases

    UniPathwayi UPA00753 ; UER00737 .
    BioCyci MetaCyc:MONOMER-13669.
    YEAST:YLR133W-MONOMER.

    Miscellaneous databases

    NextBioi 967079.

    Gene expression databases

    Genevestigatori P20485.

    Family and domain databases

    InterProi IPR026712. Cho/Etha_kinase.
    IPR007521. Choline_kin_N.
    IPR011009. Kinase-like_dom.
    [Graphical view ]
    PANTHERi PTHR22603:SF32. PTHR22603:SF32. 1 hit.
    Pfami PF04428. Choline_kin_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the yeast CKI gene encoding choline kinase and its expression in Escherichia coli."
      Hosaka K., Kodaki T., Yamashita S.
      J. Biol. Chem. 264:2053-2059(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Phosphorylation of Saccharomyces cerevisiae choline kinase on Ser30 and Ser85 by protein kinase A regulates phosphatidylcholine synthesis by the CDP-choline pathway."
      Yu Y., Sreenivas A., Ostrander D.B., Carman G.M.
      J. Biol. Chem. 277:34978-34986(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-30 AND SER-85, MUTAGENESIS OF SER-30 AND SER-85.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "Phosphorylation by casein kinase 2 regulates Nap1 localization and function."
      Calvert M.E.K., Keck K.M., Ptak C., Shabanowitz J., Hunt D.F., Pemberton L.F.
      Mol. Cell. Biol. 28:1313-1325(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NAP1, IDENTIFICATION BY MASS SPECTROMETRY.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-48; SER-51 AND THR-54, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiKICH_YEAST
    AccessioniPrimary (citable) accession number: P20485
    Secondary accession number(s): D6VYC8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 133 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3930 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3