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P20485

- KICH_YEAST

UniProt

P20485 - KICH_YEAST

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Protein

Choline kinase

Gene
CKI1, CKI, YLR133W, L3130, L9606.8
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Responsible for phosphatidylcholine synthesis via the CDP-choline pathway. Also exhibits ethanolamine kinase activity but at 14% efficiency compared with choline.

Catalytic activityi

ATP + choline = ADP + phosphocholine.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. choline kinase activity Source: SGD
  3. ethanolamine kinase activity Source: SGD
  4. protein binding Source: IntAct

GO - Biological processi

  1. CDP-choline pathway Source: GOC
  2. phosphatidylcholine biosynthetic process Source: SGD
  3. phosphatidylethanolamine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13669.
YEAST:YLR133W-MONOMER.
UniPathwayiUPA00753; UER00737.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline kinase (EC:2.7.1.32)
Gene namesi
Name:CKI1
Synonyms:CKI
Ordered Locus Names:YLR133W
ORF Names:L3130, L9606.8
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR133w.
SGDiS000004123. CKI1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi30 – 301S → A: Decrease in activity and in phosphorylation by PKA. No phosphorylation by PKA, and strong decrease in activity; when associated with A-85. 1 Publication
Mutagenesisi85 – 851S → A: No phosphorylation by PKA, and strong decrease in activity; when associated with A-30. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 582582Choline kinasePRO_0000206226Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei30 – 301Phosphoserine; by PKA1 Publication
Modified residuei48 – 481Phosphoserine1 Publication
Modified residuei51 – 511Phosphoserine1 Publication
Modified residuei54 – 541Phosphothreonine1 Publication
Modified residuei85 – 851Phosphoserine; by PKA1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP20485.
PaxDbiP20485.
PeptideAtlasiP20485.

Expressioni

Gene expression databases

GenevestigatoriP20485.

Interactioni

Subunit structurei

Monomer. Interacts with NAP1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NAP1P252935EBI-9699,EBI-11850

Protein-protein interaction databases

BioGridi31402. 78 interactions.
DIPiDIP-2617N.
IntActiP20485. 6 interactions.
MINTiMINT-422889.
STRINGi4932.YLR133W.

Structurei

3D structure databases

ProteinModelPortaliP20485.
SMRiP20485. Positions 139-487.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0510.
GeneTreeiENSGT00530000062991.
HOGENOMiHOG000246747.
KOiK00866.
OMAiKFCHNDT.
OrthoDBiEOG7WT48T.

Family and domain databases

InterProiIPR026712. Cho/Etha_kinase.
IPR007521. Choline_kin_N.
IPR011009. Kinase-like_dom.
[Graphical view]
PANTHERiPTHR22603:SF32. PTHR22603:SF32. 1 hit.
PfamiPF04428. Choline_kin_N. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.

Sequencei

Sequence statusi: Complete.

P20485-1 [UniParc]FASTAAdd to Basket

« Hide

MVQESRPGSV RSYSVGYQAR SRSSSQRRHS LTRQRSSQRL IRTISIESDV    50
SNITDDDDLR AVNEGVAGVQ LDVSETANKG PRRASATDVT DSLGSTSSEY 100
IEIPFVKETL DASLPSDYLK QDILNLIQSL KISKWYNNKK IQPVAQDMNL 150
VKISGAMTNA IFKVEYPKLP SLLLRIYGPN IDNIIDREYE LQILARLSLK 200
NIGPSLYGCF VNGRFEQFLE NSKTLTKDDI RNWKNSQRIA RRMKELHVGV 250
PLLSSERKNG SACWQKINQW LRTIEKVDQW VGDPKNIENS LLCENWSKFM 300
DIVDRYHKWL ISQEQGIEQV NKNLIFCHND AQYGNLLFTA PVMNTPSLYT 350
APSSTSLTSQ SSSLFPSSSN VIVDDIINPP KQEQSQDSKL VVIDFEYAGA 400
NPAAYDLANH LSEWMYDYNN AKAPHQCHAD RYPDKEQVLN FLYSYVSHLR 450
GGAKEPIDEE VQRLYKSIIQ WRPTVQLFWS LWAILQSGKL EKKEASTAIT 500
REEIGPNGKK YIIKTEPESP EEDFVENDDE PEAGVSIDTF DYMAYGRDKI 550
AVFWGDLIGL GIITEEECKN FSSFKFLDTS YL 582
Length:582
Mass (Da):66,317
Last modified:February 1, 1991 - v1
Checksum:i68C395B9CC120A0E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04454 Genomic DNA. Translation: AAA34499.1.
X91258 Genomic DNA. Translation: CAA62646.1.
Z73305 Genomic DNA. Translation: CAA97704.1.
U53881 Genomic DNA. Translation: AAB82396.1.
AY692779 Genomic DNA. Translation: AAT92798.1.
BK006945 Genomic DNA. Translation: DAA09444.1.
PIRiA32034.
RefSeqiNP_013234.1. NM_001182020.1.

Genome annotation databases

EnsemblFungiiYLR133W; YLR133W; YLR133W.
GeneIDi850824.
KEGGisce:YLR133W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04454 Genomic DNA. Translation: AAA34499.1 .
X91258 Genomic DNA. Translation: CAA62646.1 .
Z73305 Genomic DNA. Translation: CAA97704.1 .
U53881 Genomic DNA. Translation: AAB82396.1 .
AY692779 Genomic DNA. Translation: AAT92798.1 .
BK006945 Genomic DNA. Translation: DAA09444.1 .
PIRi A32034.
RefSeqi NP_013234.1. NM_001182020.1.

3D structure databases

ProteinModelPortali P20485.
SMRi P20485. Positions 139-487.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31402. 78 interactions.
DIPi DIP-2617N.
IntActi P20485. 6 interactions.
MINTi MINT-422889.
STRINGi 4932.YLR133W.

Proteomic databases

MaxQBi P20485.
PaxDbi P20485.
PeptideAtlasi P20485.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR133W ; YLR133W ; YLR133W .
GeneIDi 850824.
KEGGi sce:YLR133W.

Organism-specific databases

CYGDi YLR133w.
SGDi S000004123. CKI1.

Phylogenomic databases

eggNOGi COG0510.
GeneTreei ENSGT00530000062991.
HOGENOMi HOG000246747.
KOi K00866.
OMAi KFCHNDT.
OrthoDBi EOG7WT48T.

Enzyme and pathway databases

UniPathwayi UPA00753 ; UER00737 .
BioCyci MetaCyc:MONOMER-13669.
YEAST:YLR133W-MONOMER.

Miscellaneous databases

NextBioi 967079.

Gene expression databases

Genevestigatori P20485.

Family and domain databases

InterProi IPR026712. Cho/Etha_kinase.
IPR007521. Choline_kin_N.
IPR011009. Kinase-like_dom.
[Graphical view ]
PANTHERi PTHR22603:SF32. PTHR22603:SF32. 1 hit.
Pfami PF04428. Choline_kin_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the yeast CKI gene encoding choline kinase and its expression in Escherichia coli."
    Hosaka K., Kodaki T., Yamashita S.
    J. Biol. Chem. 264:2053-2059(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Phosphorylation of Saccharomyces cerevisiae choline kinase on Ser30 and Ser85 by protein kinase A regulates phosphatidylcholine synthesis by the CDP-choline pathway."
    Yu Y., Sreenivas A., Ostrander D.B., Carman G.M.
    J. Biol. Chem. 277:34978-34986(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-30 AND SER-85, MUTAGENESIS OF SER-30 AND SER-85.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "Phosphorylation by casein kinase 2 regulates Nap1 localization and function."
    Calvert M.E.K., Keck K.M., Ptak C., Shabanowitz J., Hunt D.F., Pemberton L.F.
    Mol. Cell. Biol. 28:1313-1325(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NAP1, IDENTIFICATION BY MASS SPECTROMETRY.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-48; SER-51 AND THR-54, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKICH_YEAST
AccessioniPrimary (citable) accession number: P20485
Secondary accession number(s): D6VYC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: June 11, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3930 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

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