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Protein

Choline kinase

Gene

CKI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for phosphatidylcholine synthesis via the CDP-choline pathway. Also exhibits ethanolamine kinase activity but at 14% efficiency compared with choline.

Catalytic activityi

ATP + choline = ADP + phosphocholine.

Pathwayi: phosphatidylcholine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphocholine from choline.
Proteins known to be involved in this subpathway in this organism are:
  1. Choline kinase (CKI1)
This subpathway is part of the pathway phosphatidylcholine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphocholine from choline, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • choline kinase activity Source: SGD
  • ethanolamine kinase activity Source: SGD

GO - Biological processi

  • phosphatidylcholine biosynthetic process Source: SGD
  • phosphatidylethanolamine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13669.
YEAST:YLR133W-MONOMER.
ReactomeiR-SCE-1483191. Synthesis of PC.
R-SCE-1483213. Synthesis of PE.
UniPathwayiUPA00753; UER00737.

Chemistry

SwissLipidsiSLP:000000066.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline kinase (EC:2.7.1.32)
Gene namesi
Name:CKI1
Synonyms:CKI
Ordered Locus Names:YLR133W
ORF Names:L3130, L9606.8
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR133W.
SGDiS000004123. CKI1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi30 – 301S → A: Decrease in activity and in phosphorylation by PKA. No phosphorylation by PKA, and strong decrease in activity; when associated with A-85. 1 Publication
Mutagenesisi85 – 851S → A: No phosphorylation by PKA, and strong decrease in activity; when associated with A-30. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 582582Choline kinasePRO_0000206226Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei30 – 301Phosphoserine; by PKA1 Publication
Modified residuei48 – 481PhosphoserineCombined sources
Modified residuei51 – 511PhosphoserineCombined sources
Modified residuei54 – 541PhosphothreonineCombined sources
Modified residuei85 – 851Phosphoserine; by PKA1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP20485.

PTM databases

iPTMnetiP20485.

Interactioni

Subunit structurei

Monomer. Interacts with NAP1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NAP1P252935EBI-9699,EBI-11850

Protein-protein interaction databases

BioGridi31402. 80 interactions.
DIPiDIP-2617N.
IntActiP20485. 6 interactions.
MINTiMINT-422889.

Structurei

3D structure databases

ProteinModelPortaliP20485.
SMRiP20485. Positions 139-487.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the choline/ethanolamine kinase family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000062991.
HOGENOMiHOG000246747.
InParanoidiP20485.
KOiK00866.
OMAiNHFSEWM.
OrthoDBiEOG092C0QOM.

Family and domain databases

InterProiIPR007521. Choline_kin_N.
IPR011009. Kinase-like_dom.
[Graphical view]
PfamiPF04428. Choline_kin_N. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.

Sequencei

Sequence statusi: Complete.

P20485-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQESRPGSV RSYSVGYQAR SRSSSQRRHS LTRQRSSQRL IRTISIESDV
60 70 80 90 100
SNITDDDDLR AVNEGVAGVQ LDVSETANKG PRRASATDVT DSLGSTSSEY
110 120 130 140 150
IEIPFVKETL DASLPSDYLK QDILNLIQSL KISKWYNNKK IQPVAQDMNL
160 170 180 190 200
VKISGAMTNA IFKVEYPKLP SLLLRIYGPN IDNIIDREYE LQILARLSLK
210 220 230 240 250
NIGPSLYGCF VNGRFEQFLE NSKTLTKDDI RNWKNSQRIA RRMKELHVGV
260 270 280 290 300
PLLSSERKNG SACWQKINQW LRTIEKVDQW VGDPKNIENS LLCENWSKFM
310 320 330 340 350
DIVDRYHKWL ISQEQGIEQV NKNLIFCHND AQYGNLLFTA PVMNTPSLYT
360 370 380 390 400
APSSTSLTSQ SSSLFPSSSN VIVDDIINPP KQEQSQDSKL VVIDFEYAGA
410 420 430 440 450
NPAAYDLANH LSEWMYDYNN AKAPHQCHAD RYPDKEQVLN FLYSYVSHLR
460 470 480 490 500
GGAKEPIDEE VQRLYKSIIQ WRPTVQLFWS LWAILQSGKL EKKEASTAIT
510 520 530 540 550
REEIGPNGKK YIIKTEPESP EEDFVENDDE PEAGVSIDTF DYMAYGRDKI
560 570 580
AVFWGDLIGL GIITEEECKN FSSFKFLDTS YL
Length:582
Mass (Da):66,317
Last modified:February 1, 1991 - v1
Checksum:i68C395B9CC120A0E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04454 Genomic DNA. Translation: AAA34499.1.
X91258 Genomic DNA. Translation: CAA62646.1.
Z73305 Genomic DNA. Translation: CAA97704.1.
U53881 Genomic DNA. Translation: AAB82396.1.
AY692779 Genomic DNA. Translation: AAT92798.1.
BK006945 Genomic DNA. Translation: DAA09444.1.
PIRiA32034.
RefSeqiNP_013234.1. NM_001182020.1.

Genome annotation databases

EnsemblFungiiYLR133W; YLR133W; YLR133W.
GeneIDi850824.
KEGGisce:YLR133W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04454 Genomic DNA. Translation: AAA34499.1.
X91258 Genomic DNA. Translation: CAA62646.1.
Z73305 Genomic DNA. Translation: CAA97704.1.
U53881 Genomic DNA. Translation: AAB82396.1.
AY692779 Genomic DNA. Translation: AAT92798.1.
BK006945 Genomic DNA. Translation: DAA09444.1.
PIRiA32034.
RefSeqiNP_013234.1. NM_001182020.1.

3D structure databases

ProteinModelPortaliP20485.
SMRiP20485. Positions 139-487.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31402. 80 interactions.
DIPiDIP-2617N.
IntActiP20485. 6 interactions.
MINTiMINT-422889.

Chemistry

SwissLipidsiSLP:000000066.

PTM databases

iPTMnetiP20485.

Proteomic databases

MaxQBiP20485.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR133W; YLR133W; YLR133W.
GeneIDi850824.
KEGGisce:YLR133W.

Organism-specific databases

EuPathDBiFungiDB:YLR133W.
SGDiS000004123. CKI1.

Phylogenomic databases

GeneTreeiENSGT00530000062991.
HOGENOMiHOG000246747.
InParanoidiP20485.
KOiK00866.
OMAiNHFSEWM.
OrthoDBiEOG092C0QOM.

Enzyme and pathway databases

UniPathwayiUPA00753; UER00737.
BioCyciMetaCyc:MONOMER-13669.
YEAST:YLR133W-MONOMER.
ReactomeiR-SCE-1483191. Synthesis of PC.
R-SCE-1483213. Synthesis of PE.

Miscellaneous databases

PROiP20485.

Family and domain databases

InterProiIPR007521. Choline_kin_N.
IPR011009. Kinase-like_dom.
[Graphical view]
PfamiPF04428. Choline_kin_N. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiKICH_YEAST
AccessioniPrimary (citable) accession number: P20485
Secondary accession number(s): D6VYC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: September 7, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3930 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.