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P20483 (MPIP_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
M-phase inducer phosphatase

EC=3.1.3.48
Alternative name(s):
Cdc25-like protein
Protein string
Gene names
Name:stg
Synonyms:cdc25
ORF Names:CG1395
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length479 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It may directly dephosphorylate p34(cdc2) and activate the p34(cdc2) kinase activity.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sequence similarities

Belongs to the MPI phosphatase family.

Contains 1 rhodanese domain.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
   Molecular functionHydrolase
Protein phosphatase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG2/M transition of mitotic cell cycle

Inferred from mutant phenotype PubMed 17306545PubMed 2199063. Source: FlyBase

Golgi organization

Inferred from mutant phenotype PubMed 21383842PubMed 21847093. Source: FlyBase

cell proliferation

Inferred from mutant phenotype PubMed 21383842. Source: FlyBase

cellular process

Inferred from mutant phenotype PubMed 14764878. Source: FlyBase

centriole replication

Inferred from mutant phenotype PubMed 10613895. Source: FlyBase

gastrulation

Inferred from mutant phenotype PubMed 10850494. Source: FlyBase

lateral inhibition

Inferred from mutant phenotype PubMed 19363474. Source: FlyBase

mesoderm development

Traceable author statement PubMed 10369659. Source: FlyBase

mitotic cell cycle

Inferred from mutant phenotype PubMed 14527345. Source: FlyBase

mitotic nuclear division

Inferred from electronic annotation. Source: UniProtKB-KW

negative regulation of cell size

Inferred from mutant phenotype PubMed 21847093. Source: FlyBase

negative regulation of mitotic cell cycle

Inferred from mutant phenotype PubMed 21383842. Source: FlyBase

open tracheal system development

Traceable author statement PubMed 10684581. Source: FlyBase

peptidyl-tyrosine dephosphorylation

Inferred from direct assay PubMed 1655274. Source: GOC

peripheral nervous system development

Traceable author statement PubMed 11102367. Source: FlyBase

positive regulation of cell proliferation

Inferred from mutant phenotype PubMed 21847093. Source: FlyBase

positive regulation of mitotic cell cycle

Inferred from mutant phenotype PubMed 21847093. Source: FlyBase

positive regulation of mitotic cell cycle, embryonic

Inferred from mutant phenotype PubMed 22483720. Source: FlyBase

protein dephosphorylation

Inferred from direct assay PubMed 1655274. Source: FlyBase

regulation of cell diameter

Inferred from mutant phenotype PubMed 21383842. Source: FlyBase

regulation of mitotic cell cycle

Inferred from mutant phenotype PubMed 12077326. Source: FlyBase

   Cellular_componentchromosome

Inferred from direct assay PubMed 7510257. Source: FlyBase

nucleus

Inferred from direct assay PubMed 7510257. Source: FlyBase

   Molecular_functionprotein tyrosine phosphatase activity

Inferred from direct assay PubMed 1655274. Source: FlyBase

protein tyrosine/serine/threonine phosphatase activity

Non-traceable author statement PubMed 10908587. Source: FlyBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 479479M-phase inducer phosphatase
PRO_0000198658

Regions

Domain316 – 432117Rhodanese

Sites

Active site3791 By similarity

Amino acid modifications

Modified residue4551Phosphoserine Ref.6

Experimental info

Sequence conflict2281A → T in AAA28916. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P20483 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 68483F3A285962CC

FASTA47954,095
        10         20         30         40         50         60 
MLWETIVEEN NCSMDCNISN NTSSSSSINK MSGSRRARRS LELMSMDQEE LSFYDDDVVP 

        70         80         90        100        110        120 
QDQQRSASPE LMGLLSPEGS PQRFQIVRQP KILPAMGVSS DHTPARSFRI FNSLSSTCSM 

       130        140        150        160        170        180 
ESSMDDEYME LFEMESQSQQ TALGFPSGLN SLISGQIKEQ PAAKSPAGLS MRRPSVRRCL 

       190        200        210        220        230        240 
SMTESNTNST TTPPPKTPET ARDCFKRPEP PASANCSPIQ SKRHRCAAVE KENCPAPSPL 

       250        260        270        280        290        300 
SQVTISHPPP LRKCMSLNDA EIMSALARSE NRNEPELIGD FSKAYALPLM EGRHRDLKSI 

       310        320        330        340        350        360 
SSETVARLLK GEFSDKVASY RIIDCRYPYE FEGGHIEGAK NLYTTEQILD EFLTVQQTEL 

       370        380        390        400        410        420 
QQQQNAESGH KRNIIIFHCE FSSERGPKMS RFLRNLDRER NTNAYPALHY PEIYLLHNGY 

       430        440        450        460        470 
KEFFESHVEL CEPHAYRTML DPAYNEAYRH FRAKSKSWNG DGLGGATGRL KKSRSRLML 

« Hide

References

« Hide 'large scale' references
[1]"Genetic control of cell division patterns in the Drosophila embryo."
Edgar B.A., O'Farrell P.H.
Cell 57:177-187(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Complementation of fission yeast cdc2ts and cdc25ts mutants identifies two cell cycle genes from Drosophila: a cdc2 homologue and string."
Jimenez J., Alphey L., Nurse P., Glover D.M.
EMBO J. 9:3565-3571(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[4]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[5]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Embryo.
[6]"Phosphoproteome analysis of Drosophila melanogaster embryos."
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-455, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M24909 mRNA. Translation: AAA28916.1.
X57495 mRNA. Translation: CAA40732.1.
AE014297 Genomic DNA. Translation: AAF56885.1.
AY069704 mRNA. Translation: AAL39849.1.
PIRA32290.
RefSeqNP_001263066.1. NM_001276137.1.
NP_524547.1. NM_079823.3.
UniGeneDm.3208.

3D structure databases

ProteinModelPortalP20483.
SMRP20483. Positions 277-439.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid68333. 22 interactions.
DIPDIP-19931N.
IntActP20483. 2 interactions.
MINTMINT-848308.
STRING7227.FBpp0084766.

Proteomic databases

PRIDEP20483.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0085397; FBpp0084766; FBgn0003525.
GeneID43466.
KEGGdme:Dmel_CG1395.

Organism-specific databases

CTD43466.
FlyBaseFBgn0003525. stg.

Phylogenomic databases

eggNOGCOG5105.
GeneTreeENSGT00390000018747.
HOGENOMHOG000263899.
InParanoidP20483.
KOK16723.
OMAYMELFEM.
OrthoDBEOG757D02.
PhylomeDBP20483.

Gene expression databases

BgeeP20483.

Family and domain databases

Gene3D3.40.250.10. 1 hit.
InterProIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamPF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSPR00716. MPIPHPHTASE.
SMARTSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMSSF52821. SSF52821. 1 hit.
PROSITEPS50206. RHODANESE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSC6orf15. drosophila.
GenomeRNAi43466.
NextBio834076.

Entry information

Entry nameMPIP_DROME
AccessionPrimary (citable) accession number: P20483
Secondary accession number(s): Q9VAL9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: December 1, 2000
Last modified: July 9, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase