P20483 (MPIP_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
M-phase inducer phosphatase
EC=3.1.3.48

Alternative name(s):
Cdc25-like protein
Protein string

Gene names

Name:	stg
Synonyms:	cdc25
ORF Names:	CG1395

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It may directly dephosphorylate p34(cdc2) and activate the p34(cdc2) kinase activity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Sequence similarities	Belongs to the MPI phosphatase family. Contains 1 rhodanese domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G2/M transition of mitotic cell cycle Inferred from mutant phenotype PubMed 17306545PubMed 2199063. Source: FlyBase Golgi organization Inferred from mutant phenotype PubMed 21383842PubMed 21847093. Source: FlyBase cell division Inferred from electronic annotation. Source: UniProtKB-KW cell proliferation Inferred from mutant phenotype PubMed 21383842. Source: FlyBase centriole replication Inferred from mutant phenotype PubMed 10613895. Source: FlyBase gastrulation Inferred from mutant phenotype PubMed 10850494. Source: FlyBase lateral inhibition Inferred from mutant phenotype PubMed 19363474. Source: FlyBase mesoderm development Traceable author statement PubMed 10369659. Source: FlyBase mitosis Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of cell size Inferred from mutant phenotype PubMed 21847093. Source: FlyBase negative regulation of mitotic cell cycle Inferred from mutant phenotype PubMed 21383842. Source: FlyBase open tracheal system development Traceable author statement PubMed 10684581. Source: FlyBase peripheral nervous system development Traceable author statement PubMed 11102367. Source: FlyBase positive regulation of cell proliferation Inferred from mutant phenotype PubMed 21847093. Source: FlyBase positive regulation of mitotic cell cycle, embryonic Inferred from mutant phenotype PubMed 22483720. Source: FlyBase regulation of cell diameter Inferred from mutant phenotype PubMed 21383842. Source: FlyBase
Cellular_component	chromosome Inferred from direct assay PubMed 7510257. Source: FlyBase nucleus Inferred from direct assay PubMed 7510257. Source: FlyBase
Molecular_function	protein tyrosine phosphatase activity Inferred from direct assay PubMed 1655274. Source: FlyBase protein tyrosine/serine/threonine phosphatase activity Non-traceable author statement PubMed 10908587. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	M-phase inducer phosphatase		PRO_0000198658
Regions
	Domain	316 – 432	117	Rhodanese
Sites
	Active site	379	1	By similarity
Amino acid modifications
	Modified residue	455	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	228	1	A → T in AAA28916. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P20483 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 68483F3A285962CC	FASTA	479	54,095
	10 20 30 40 50 60 MLWETIVEEN NCSMDCNISN NTSSSSSINK MSGSRRARRS LELMSMDQEE LSFYDDDVVP 70 80 90 100 110 120 QDQQRSASPE LMGLLSPEGS PQRFQIVRQP KILPAMGVSS DHTPARSFRI FNSLSSTCSM 130 140 150 160 170 180 ESSMDDEYME LFEMESQSQQ TALGFPSGLN SLISGQIKEQ PAAKSPAGLS MRRPSVRRCL 190 200 210 220 230 240 SMTESNTNST TTPPPKTPET ARDCFKRPEP PASANCSPIQ SKRHRCAAVE KENCPAPSPL 250 260 270 280 290 300 SQVTISHPPP LRKCMSLNDA EIMSALARSE NRNEPELIGD FSKAYALPLM EGRHRDLKSI 310 320 330 340 350 360 SSETVARLLK GEFSDKVASY RIIDCRYPYE FEGGHIEGAK NLYTTEQILD EFLTVQQTEL 370 380 390 400 410 420 QQQQNAESGH KRNIIIFHCE FSSERGPKMS RFLRNLDRER NTNAYPALHY PEIYLLHNGY 430 440 450 460 470 KEFFESHVEL CEPHAYRTML DPAYNEAYRH FRAKSKSWNG DGLGGATGRL KKSRSRLML

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic control of cell division patterns in the Drosophila embryo." Edgar B.A., O'Farrell P.H. Cell 57:177-187(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complementation of fission yeast cdc2ts and cdc25ts mutants identifies two cell cycle genes from Drosophila: a cdc2 homologue and string." Jimenez J., Alphey L., Nurse P., Glover D.M. EMBO J. 9:3565-3571(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-455, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M24909 mRNA. Translation: AAA28916.1. X57495 mRNA. Translation: CAA40732.1. AE014297 Genomic DNA. Translation: AAF56885.1. AY069704 mRNA. Translation: AAL39849.1.
PIR	A32290.
RefSeq	NP_001263066.1. NM_001276137.1. NP_524547.1. NM_079823.3.
UniGene	Dm.3208.
3D structure databases
ProteinModelPortal	P20483.
SMR	P20483. Positions 277-439.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-19931N.
IntAct	P20483. 2 interactions.
MINT	MINT-848308.
STRING	7227.FBpp0084766.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0085397; FBpp0084766; FBgn0003525.
GeneID	43466.
KEGG	dme:Dmel_CG1395.
Organism-specific databases
CTD	43466.
FlyBase	FBgn0003525. stg.
Phylogenomic databases
eggNOG	COG5105.
GeneTree	ENSGT00390000018747.
HOGENOM	HOG000263899.
InParanoid	P20483.
KO	K16723.
OMA	RHRDLKS.
OrthoDB	EOG4RBP0Z.
PhylomeDB	P20483.
Gene expression databases
Bgee	P20483.
GermOnline	CG1395. Drosophila melanogaster.
Family and domain databases
Gene3D	3.40.250.10. 1 hit.
InterPro	IPR000751. MPI_Phosphatase. IPR001763. Rhodanese-like_dom. [Graphical view]
PANTHER	PTHR10828. PTHR10828. 1 hit.
Pfam	PF00581. Rhodanese. 1 hit. [Graphical view]
PRINTS	PR00716. MPIPHPHTASE.
SMART	SM00450. RHOD. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	C6orf15. drosophila.
GenomeRNAi	43466.
NextBio	834076.

Entry information

Entry name

MPIP_DROME

Accession

Primary (citable) accession number: P20483
Secondary accession number(s): Q9VAL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 123 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project