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P20481

- BUCC_APLCA

UniProt

P20481 - BUCC_APLCA

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Protein

Buccalin

Gene
N/A
Organism
Aplysia californica (California sea hare)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Modulatory neuropeptide, acting presynaptically on nerve terminals to inhibit acetylcholine release.1 Publication

GO - Biological processi

  1. neuropeptide signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Neuropeptide

Names & Taxonomyi

Protein namesi
OrganismiAplysia californica (California sea hare)
Taxonomic identifieri6500 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaHeterobranchiaEuthyneuraEuopisthobranchiaAplysiomorphaAplysioideaAplysiidaeAplysia

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 Reviewed predictionAdd
BLAST
Propeptidei26 – 6237 Reviewed predictionPRO_0000001881Add
BLAST
Peptidei63 – 7412Buccalin-DPRO_0000001882Add
BLAST
Peptidei78 – 8811Buccalin-EPRO_0000001883Add
BLAST
Peptidei93 – 10210Buccalin-FPRO_0000001884
Peptidei106 – 11611Buccalin-GPRO_0000001885Add
BLAST
Peptidei120 – 12910Buccalin-H Reviewed predictionPRO_0000001886
Peptidei133 – 14311Buccalin-A1 PublicationPRO_0000001887Add
BLAST
Peptidei147 – 15711Buccalin-APRO_0000001888Add
BLAST
Peptidei161 – 17111Buccalin-APRO_0000001889Add
BLAST
Peptidei175 – 18511Buccalin-APRO_0000001890Add
BLAST
Peptidei189 – 19911Buccalin-APRO_0000001891Add
BLAST
Peptidei203 – 21311Buccalin-APRO_0000001892Add
BLAST
Peptidei217 – 22711Buccalin-I Reviewed predictionPRO_0000001893Add
BLAST
Peptidei231 – 24111Buccalin-J Reviewed predictionPRO_0000001894Add
BLAST
Peptidei245 – 25410Buccalin-K Reviewed predictionPRO_0000001895
Peptidei258 – 26710Buccalin-L Reviewed predictionPRO_0000001896
Peptidei271 – 28111Buccalin-J Reviewed predictionPRO_0000001897Add
BLAST
Peptidei285 – 29410Buccalin-L Reviewed predictionPRO_0000001898
Peptidei298 – 30710Buccalin-L Reviewed predictionPRO_0000001899
Peptidei311 – 32111Buccalin-BPRO_0000001900Add
BLAST
Peptidei325 – 33511Buccalin-MPRO_0000001901Add
BLAST
Peptidei339 – 34911Buccalin gene-predicted acidic peptide A Reviewed predictionPRO_0000001902Add
BLAST
Peptidei353 – 36311Buccalin-NPRO_0000001903Add
BLAST
Peptidei367 – 37711Buccalin-B1 PublicationPRO_0000001904Add
BLAST
Peptidei381 – 39111Buccalin-O Reviewed predictionPRO_0000001905Add
BLAST
Peptidei396 – 40510Buccalin-P Reviewed predictionPRO_0000001906
Peptidei409 – 41911Buccalin-QPRO_0000001907Add
BLAST
Peptidei424 – 43310Buccalin-RPRO_0000001908
Peptidei437 – 44711Buccalin-C1 PublicationPRO_0000001909Add
BLAST
Peptidei451 – 46111Buccalin-CPRO_0000001910Add
BLAST
Peptidei465 – 47511Buccalin-SPRO_0000001911Add
BLAST
Peptidei479 – 49214Buccalin gene-predicted acidic peptide B Reviewed predictionPRO_0000001912Add
BLAST
Propeptidei495 – 50511 Reviewed predictionPRO_0000001913Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei74 – 741Valine amide Reviewed prediction
Modified residuei88 – 881Leucine amide Reviewed prediction
Modified residuei102 – 1021Leucine amide
Modified residuei106 – 1061Pyrrolidone carboxylic acid Reviewed prediction
Modified residuei116 – 1161Isoleucine amide
Modified residuei129 – 1291Leucine amide Reviewed prediction
Modified residuei143 – 1431Leucine amide1 Publication
Modified residuei157 – 1571Leucine amide1 Publication
Modified residuei171 – 1711Leucine amide1 Publication
Modified residuei185 – 1851Leucine amide1 Publication
Modified residuei199 – 1991Leucine amide1 Publication
Modified residuei213 – 2131Leucine amide1 Publication
Modified residuei227 – 2271Leucine amide Reviewed prediction
Modified residuei241 – 2411Leucine amide Reviewed prediction
Modified residuei254 – 2541Leucine amide Reviewed prediction
Modified residuei267 – 2671Leucine amide
Modified residuei281 – 2811Leucine amide Reviewed prediction
Modified residuei294 – 2941Leucine amide Reviewed prediction
Modified residuei307 – 3071Leucine amide Reviewed prediction
Modified residuei321 – 3211Leucine amide
Modified residuei335 – 3351Leucine amide
Modified residuei349 – 3491Glutamic acid 1-amide Reviewed prediction
Modified residuei363 – 3631Leucine amide
Modified residuei377 – 3771Leucine amide
Modified residuei391 – 3911Leucine amide Reviewed prediction
Modified residuei405 – 4051Leucine amide Reviewed prediction
Modified residuei419 – 4191Leucine amide
Modified residuei433 – 4331Leucine amide
Modified residuei447 – 4471Isoleucine amide
Modified residuei461 – 4611Isoleucine amide
Modified residuei465 – 4651Pyrrolidone carboxylic acid Reviewed prediction
Modified residuei475 – 4751Leucine amide

Keywords - PTMi

Amidation, Cleavage on pair of basic residues, Pyrrolidone carboxylic acid

Expressioni

Tissue specificityi

Cholinergic motor neuron B15 innervating buccal muscles in Aplysia.

Family & Domainsi

Keywords - Domaini

Signal

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20481-1 [UniParc]FASTAAdd to Basket

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MAHHRGHRHI LLYVSLALSL GLALAEDATD PSDDTGSFDD VEAVSEEADL    50
DPYSMSQELN KRPNVDPYSY LPSVGKRAFD HYGFTGGLGK RKIDHFGFVG 100
GLGKRQIDPL GFSGGIGKRY DSFAYSAGLG KRGMDSLAFS GGLGKRGMDS 150
LAFSGGLGKR GMDSLAFSGG LGKRGMDSLA FSGGLGKRGM DSLAFSGGLG 200
KRGMDSLAFS GGLGKRGMDS FTFAPGLGKR GMDSLAFAGG LGKRMDGFAF 250
APGLGKRMDS FAFAPGLGKR GMDSLAFAGG LGKRMDSFAF APGLGKRMDS 300
FAFAPGLGKR GLDRYGFVGG LGKRGMDHFA FTGGLGKRDS GEASGDLEEG 350
KRGLDAYSFT GALGKRGLDR YGFVGGLGKR GMDDFAFSPG LGKKRMDSFM 400
FGSRLGKRGM DRFSFSGHLG KRKMDQFSFG PGLGKRGFDH YGFTGGIGKR 450
GFDHYGFTGG IGKRQLDPML FSGRLGKRSS SEQEEEDVRQ VEKRSTTEEQ 500
SSKSL 505
Length:505
Mass (Da):53,517
Last modified:October 1, 1996 - v2
Checksum:iFC17DB022C818D51
GO

Mass spectrometryi

Molecular mass is 1059.1±0.4 Da from positions 93 - 102. Determined by MALDI. 1 Publication
Molecular mass is 1101.6±0.0 Da from positions 106 - 116. Determined by MALDI. 1 Publication
Molecular mass is 1052.8±0.3 Da from positions 133 - 143. Determined by MALDI. Buccalin-A.1 Publication
Molecular mass is 1053.6±0.1 Da from positions 258 - 267. Determined by MALDI. 1 Publication
Molecular mass is 1151.9±0.3 Da from positions 311 - 321. Determined by MALDI. Buccalin-B.1 Publication
Molecular mass is 1150.6±0.1 Da from positions 325 - 335. Determined by MALDI. 1 Publication
Molecular mass is 1112.9±0.4 Da from positions 353 - 363. Determined by MALDI. 1 Publication
Molecular mass is 1251.6±0.0 Da from positions 409 - 419. Determined by MALDI. 1 Publication
Molecular mass is 1096.7±0.2 Da from positions 424 - 433. Determined by MALDI. 1 Publication
Molecular mass is 1168.7±0.2 Da from positions 437 - 447. Determined by MALDI. Buccalin-C.1 Publication
Molecular mass is 1274.9±0.2 Da from positions 465 - 475. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S64298 mRNA. Translation: AAB27696.2.
PIRiA35594.
RefSeqiNP_001191649.1. NM_001204720.1.
UniGeneiAcl.5094.

Genome annotation databases

GeneIDi100533443.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S64298 mRNA. Translation: AAB27696.2 .
PIRi A35594.
RefSeqi NP_001191649.1. NM_001204720.1.
UniGenei Acl.5094.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100533443.

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "The buccalin-related neuropeptides: isolation and characterization of an Aplysia cDNA clone encoding a family of peptide cotransmitters."
    Miller M.W., Beushausen S., Cropper E.C., Eisinger K., Stamm S., Vilim F.S., Vitek A., Zajc A., Kupfermann I., Brosius J., Weiss K.R.
    J. Neurosci. 13:3346-3357(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 437-447 AND 451-461.
  2. "Structure and action of buccalin: a modulatory neuropeptide localized to an identified small cardioactive peptide-containing cholinergic motor neuron of Aplysia californica."
    Cropper E.C., Miller M.W., Tenenbaum R., Kolks M.A.G., Kupfermann I., Weiss K.R.
    Proc. Natl. Acad. Sci. U.S.A. 85:6177-6181(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 133-143; 147-157; 161-171; 175-185; 189-199 AND 203-213, AMIDATION AT LEU-143; LEU-157; LEU-171; LEU-185; LEU-199 AND LEU-213.
  3. "Structure, localization, and action of buccalin B: a bioactive peptide from Aplysia."
    Vilim F.S., Cropper E.C., Rosen S.C., Tenenbaum R., Kupfermann I., Weiss K.R.
    Peptides 15:959-969(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 367-377, FUNCTION.
  4. "Mass spectrometric survey of interganglionically transported peptides in Aplysia."
    Li L., Moroz T.P., Garden R.W., Floyd P.D., Weiss K.R., Sweedler J.V.
    Peptides 19:1425-1433(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: MASS SPECTROMETRY.

Entry informationi

Entry nameiBUCC_APLCA
AccessioniPrimary (citable) accession number: P20481
Secondary accession number(s): Q9TWM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: October 1, 1996
Last modified: April 3, 2013
This is version 50 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

External Data

Dasty 3