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Protein

Buccalin

Gene
N/A
Organism
Aplysia californica (California sea hare)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Modulatory neuropeptide, acting presynaptically on nerve terminals to inhibit acetylcholine release.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Neuropeptide

Names & Taxonomyi

Protein namesi
OrganismiAplysia californica (California sea hare)
Taxonomic identifieri6500 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaHeterobranchiaEuthyneuraEuopisthobranchiaAplysiomorphaAplysioideaAplysiidaeAplysia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
PropeptideiPRO_000000188126 – 62Sequence analysisAdd BLAST37
PeptideiPRO_000000188263 – 74Buccalin-DAdd BLAST12
PeptideiPRO_000000188378 – 88Buccalin-EAdd BLAST11
PeptideiPRO_000000188493 – 102Buccalin-F10
PeptideiPRO_0000001885106 – 116Buccalin-GAdd BLAST11
PeptideiPRO_0000001886120 – 129Buccalin-HSequence analysis10
PeptideiPRO_0000001887133 – 143Buccalin-AAdd BLAST11
PeptideiPRO_0000001888147 – 157Buccalin-AAdd BLAST11
PeptideiPRO_0000001889161 – 171Buccalin-AAdd BLAST11
PeptideiPRO_0000001890175 – 185Buccalin-AAdd BLAST11
PeptideiPRO_0000001891189 – 199Buccalin-AAdd BLAST11
PeptideiPRO_0000001892203 – 213Buccalin-AAdd BLAST11
PeptideiPRO_0000001893217 – 227Buccalin-ISequence analysisAdd BLAST11
PeptideiPRO_0000001894231 – 241Buccalin-JSequence analysisAdd BLAST11
PeptideiPRO_0000001895245 – 254Buccalin-KSequence analysis10
PeptideiPRO_0000001896258 – 267Buccalin-LSequence analysis10
PeptideiPRO_0000001897271 – 281Buccalin-JSequence analysisAdd BLAST11
PeptideiPRO_0000001898285 – 294Buccalin-LSequence analysis10
PeptideiPRO_0000001899298 – 307Buccalin-LSequence analysis10
PeptideiPRO_0000001900311 – 321Buccalin-BAdd BLAST11
PeptideiPRO_0000001901325 – 335Buccalin-MAdd BLAST11
PeptideiPRO_0000001902339 – 349Buccalin gene-predicted acidic peptide ASequence analysisAdd BLAST11
PeptideiPRO_0000001903353 – 363Buccalin-NAdd BLAST11
PeptideiPRO_0000001904367 – 377Buccalin-BAdd BLAST11
PeptideiPRO_0000001905381 – 391Buccalin-OSequence analysisAdd BLAST11
PeptideiPRO_0000001906396 – 405Buccalin-PSequence analysis10
PeptideiPRO_0000001907409 – 419Buccalin-QAdd BLAST11
PeptideiPRO_0000001908424 – 433Buccalin-R10
PeptideiPRO_0000001909437 – 447Buccalin-CAdd BLAST11
PeptideiPRO_0000001910451 – 461Buccalin-CAdd BLAST11
PeptideiPRO_0000001911465 – 475Buccalin-SAdd BLAST11
PeptideiPRO_0000001912479 – 492Buccalin gene-predicted acidic peptide BSequence analysisAdd BLAST14
PropeptideiPRO_0000001913495 – 505Sequence analysisAdd BLAST11

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei74Valine amideSequence analysis1
Modified residuei88Leucine amideSequence analysis1
Modified residuei102Leucine amide1
Modified residuei106Pyrrolidone carboxylic acidSequence analysis1
Modified residuei116Isoleucine amide1
Modified residuei129Leucine amideSequence analysis1
Modified residuei143Leucine amide1 Publication1
Modified residuei157Leucine amide1 Publication1
Modified residuei171Leucine amide1 Publication1
Modified residuei185Leucine amide1 Publication1
Modified residuei199Leucine amide1 Publication1
Modified residuei213Leucine amide1 Publication1
Modified residuei227Leucine amideSequence analysis1
Modified residuei241Leucine amideSequence analysis1
Modified residuei254Leucine amideSequence analysis1
Modified residuei267Leucine amide1
Modified residuei281Leucine amideSequence analysis1
Modified residuei294Leucine amideSequence analysis1
Modified residuei307Leucine amideSequence analysis1
Modified residuei321Leucine amide1
Modified residuei335Leucine amide1
Modified residuei349Glutamic acid 1-amideSequence analysis1
Modified residuei363Leucine amide1
Modified residuei377Leucine amide1
Modified residuei391Leucine amideSequence analysis1
Modified residuei405Leucine amideSequence analysis1
Modified residuei419Leucine amide1
Modified residuei433Leucine amide1
Modified residuei447Isoleucine amide1
Modified residuei461Isoleucine amide1
Modified residuei465Pyrrolidone carboxylic acidSequence analysis1
Modified residuei475Leucine amide1

Keywords - PTMi

Amidation, Cleavage on pair of basic residues, Pyrrolidone carboxylic acid

Proteomic databases

PRIDEiP20481.

Expressioni

Tissue specificityi

Cholinergic motor neuron B15 innervating buccal muscles in Aplysia.

Family & Domainsi

Keywords - Domaini

Signal

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20481-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHHRGHRHI LLYVSLALSL GLALAEDATD PSDDTGSFDD VEAVSEEADL
60 70 80 90 100
DPYSMSQELN KRPNVDPYSY LPSVGKRAFD HYGFTGGLGK RKIDHFGFVG
110 120 130 140 150
GLGKRQIDPL GFSGGIGKRY DSFAYSAGLG KRGMDSLAFS GGLGKRGMDS
160 170 180 190 200
LAFSGGLGKR GMDSLAFSGG LGKRGMDSLA FSGGLGKRGM DSLAFSGGLG
210 220 230 240 250
KRGMDSLAFS GGLGKRGMDS FTFAPGLGKR GMDSLAFAGG LGKRMDGFAF
260 270 280 290 300
APGLGKRMDS FAFAPGLGKR GMDSLAFAGG LGKRMDSFAF APGLGKRMDS
310 320 330 340 350
FAFAPGLGKR GLDRYGFVGG LGKRGMDHFA FTGGLGKRDS GEASGDLEEG
360 370 380 390 400
KRGLDAYSFT GALGKRGLDR YGFVGGLGKR GMDDFAFSPG LGKKRMDSFM
410 420 430 440 450
FGSRLGKRGM DRFSFSGHLG KRKMDQFSFG PGLGKRGFDH YGFTGGIGKR
460 470 480 490 500
GFDHYGFTGG IGKRQLDPML FSGRLGKRSS SEQEEEDVRQ VEKRSTTEEQ

SSKSL
Length:505
Mass (Da):53,517
Last modified:October 1, 1996 - v2
Checksum:iFC17DB022C818D51
GO

Mass spectrometryi

Molecular mass is 1059.1±0.4 Da from positions 93 - 102. Determined by MALDI. 1 Publication
Molecular mass is 1101.6±0.0 Da from positions 106 - 116. Determined by MALDI. 1 Publication
Molecular mass is 1052.8±0.3 Da from positions 133 - 143. Determined by MALDI. Buccalin-A.1 Publication
Molecular mass is 1053.6±0.1 Da from positions 258 - 267. Determined by MALDI. 1 Publication
Molecular mass is 1151.9±0.3 Da from positions 311 - 321. Determined by MALDI. Buccalin-B.1 Publication
Molecular mass is 1150.6±0.1 Da from positions 325 - 335. Determined by MALDI. 1 Publication
Molecular mass is 1112.9±0.4 Da from positions 353 - 363. Determined by MALDI. 1 Publication
Molecular mass is 1251.6±0.0 Da from positions 409 - 419. Determined by MALDI. 1 Publication
Molecular mass is 1096.7±0.2 Da from positions 424 - 433. Determined by MALDI. 1 Publication
Molecular mass is 1168.7±0.2 Da from positions 437 - 447. Determined by MALDI. Buccalin-C.1 Publication
Molecular mass is 1274.9±0.2 Da from positions 465 - 475. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S64298 mRNA. Translation: AAB27696.2.
PIRiA35594.
RefSeqiNP_001191649.1. NM_001204720.1.
UniGeneiAcl.5094.

Genome annotation databases

GeneIDi100533443.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S64298 mRNA. Translation: AAB27696.2.
PIRiA35594.
RefSeqiNP_001191649.1. NM_001204720.1.
UniGeneiAcl.5094.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP20481.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100533443.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiBUCC_APLCA
AccessioniPrimary (citable) accession number: P20481
Secondary accession number(s): Q9TWM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: October 1, 1996
Last modified: October 5, 2016
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.