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Protein

Protein claret segregational

Gene

ncd

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NCD is required for normal chromosomal segregation in meiosis, in females, and in early mitotic divisions of the embryo. The NCD motor activity is directed toward the microtubule's minus end.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi434 – 441ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: GO_Central
  • ATP binding Source: UniProtKB-KW
  • ATP-dependent microtubule motor activity, minus-end-directed Source: FlyBase
  • microtubule motor activity Source: FlyBase
  • protein homodimerization activity Source: FlyBase

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • centrosome duplication Source: FlyBase
  • centrosome organization Source: FlyBase
  • chromosome segregation Source: FlyBase
  • distributive segregation Source: FlyBase
  • meiotic spindle organization Source: FlyBase
  • microtubule-based movement Source: FlyBase
  • microtubule bundle formation Source: FlyBase
  • minus-end directed microtubule sliding Source: FlyBase
  • mitotic centrosome separation Source: FlyBase
  • mitotic spindle assembly Source: FlyBase
  • mitotic spindle elongation Source: FlyBase
  • mitotic spindle organization Source: FlyBase
  • mRNA transport Source: FlyBase
  • regulation of mitotic spindle assembly Source: FlyBase
  • regulation of mitotic spindle elongation Source: FlyBase
  • spindle assembly involved in female meiosis Source: FlyBase
  • spindle assembly involved in meiosis Source: FlyBase
  • spindle organization Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-983189. Kinesins.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein claret segregational
Gene namesi
Name:ncd
Synonyms:CA(ND)
ORF Names:CG7831
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0002924. ncd.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: FlyBase
  • cytosol Source: Reactome
  • kinesin complex Source: FlyBase
  • meiotic spindle Source: FlyBase
  • mitotic spindle microtubule Source: FlyBase
  • nucleus Source: FlyBase
  • spindle Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi556V → F in ncd(D); reduces motor velocity and shows abnormal chromosomal segregation. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001253781 – 700Protein claret segregationalAdd BLAST700

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei94Phosphoserine1 Publication1
Modified residuei96Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP20480.
PRIDEiP20480.

PTM databases

iPTMnetiP20480.

Expressioni

Gene expression databases

BgeeiFBgn0002924.
ExpressionAtlasiP20480. baseline.
GenevisibleiP20480. DM.

Interactioni

GO - Molecular functioni

  • protein homodimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi68381. 17 interactors.
DIPiDIP-21302N.
IntActiP20480. 6 interactors.
MINTiMINT-949768.
STRINGi7227.FBpp0084900.

Structurei

Secondary structure

1700
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi293 – 345Combined sources53
Beta strandi348 – 355Combined sources8
Helixi360 – 362Combined sources3
Beta strandi363 – 365Combined sources3
Beta strandi369 – 374Combined sources6
Beta strandi377 – 381Combined sources5
Helixi385 – 388Combined sources4
Helixi389 – 391Combined sources3
Beta strandi395 – 397Combined sources3
Beta strandi399 – 402Combined sources4
Helixi408 – 412Combined sources5
Turni413 – 415Combined sources3
Helixi416 – 423Combined sources8
Beta strandi428 – 435Combined sources8
Helixi440 – 444Combined sources5
Beta strandi448 – 451Combined sources4
Helixi453 – 468Combined sources16
Helixi469 – 471Combined sources3
Beta strandi473 – 485Combined sources13
Beta strandi488 – 491Combined sources4
Beta strandi502 – 504Combined sources3
Beta strandi511 – 514Combined sources4
Beta strandi520 – 522Combined sources3
Helixi525 – 538Combined sources14
Beta strandi543 – 545Combined sources3
Helixi547 – 550Combined sources4
Beta strandi554 – 565Combined sources12
Turni566 – 569Combined sources4
Beta strandi570 – 580Combined sources11
Turni598 – 600Combined sources3
Helixi601 – 614Combined sources14
Helixi622 – 624Combined sources3
Helixi626 – 631Combined sources6
Helixi632 – 634Combined sources3
Beta strandi635 – 638Combined sources4
Beta strandi640 – 647Combined sources8
Helixi651 – 653Combined sources3
Helixi654 – 670Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CZ7X-ray2.90A/B/C/D295-700[»]
1N6MX-ray2.50A/B293-700[»]
1OZXmodel-A345-667[»]
1SYJmodel-A345-667[»]
1SYPmodel-A345-667[»]
1SZ4model-A345-667[»]
1SZ5model-A345-667[»]
2NCDX-ray2.50A281-700[»]
3L1CX-ray2.75A/B293-674[»]
3U06X-ray2.35A/B293-700[»]
5HNWelectron microscopy6.60K325-348[»]
K664-700[»]
5HNXelectron microscopy6.60K325-348[»]
K664-700[»]
5HNYelectron microscopy6.30K325-348[»]
K447-493[»]
K695-700[»]
5HNZelectron microscopy5.80K325-348[»]
K430-700[»]
ProteinModelPortaliP20480.
SMRiP20480.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20480.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini348 – 670Kinesin motorPROSITE-ProRule annotationAdd BLAST323

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili196 – 346Sequence analysisAdd BLAST151

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. NCD subfamily.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0239. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00550000074610.
InParanoidiP20480.
KOiK10405.
OMAiGWEYEIR.
OrthoDBiEOG091G0DJX.
PhylomeDBiP20480.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 3 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20480-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESRLPKPSG LKKPQMPIKT VLPTDRIRAG LGGGAAGAGA FNVNANQTYC
60 70 80 90 100
GNLLPPLSRD LNNLPQVLER RGGGARAASP EPMKLGHRAK LRRSRSACDI
110 120 130 140 150
NELRGNKRTA AAPSLPSIPS KVSRLGGALT VSSQRLVRPA APSSITATAV
160 170 180 190 200
KRPPVTRPAP RAAGGAAAKK PAGTGAAASS GAAAAAPKRI APYDFKARFH
210 220 230 240 250
DLLEKHKVLK TKYEKQTEDM GELESMPQQL EETQNKLIET ESSLKNTQSD
260 270 280 290 300
NECLQRQVKQ HTAKIETITS TLGRTKEELS ELQAIHEKVK TEHAALSTEV
310 320 330 340 350
VHLRQRTEEL LRCNEQQAAE LETCKEQLFQ SNMERKELHN TVMDLRGNIR
360 370 380 390 400
VFCRIRPPLE SEENRMCCTW TYHDESTVEL QSIDAQAKSK MGQQIFSFDQ
410 420 430 440 450
VFHPLSSQSD IFEMVSPLIQ SALDGYNICI FAYGQTGSGK TYTMDGVPES
460 470 480 490 500
VGVIPRTVDL LFDSIRGYRN LGWEYEIKAT FLEIYNEVLY DLLSNEQKDM
510 520 530 540 550
EIRMAKNNKN DIYVSNITEE TVLDPNHLRH LMHTAKMNRA TASTAGNERS
560 570 580 590 600
SRSHAVTKLE LIGRHAEKQE ISVGSINLVD LAGSESPKTS TRMTETKNIN
610 620 630 640 650
RSLSELTNVI LALLQKQDHI PYRNSKLTHL LMPSLGGNSK TLMFINVSPF
660 670 680 690 700
QDCFQESVKS LRFAASVNSC KMTKAKRNRY LNNSVANSST QSNNSGSFDK
Length:700
Mass (Da):77,474
Last modified:February 1, 1991 - v1
Checksum:iADE043CBCE7FD561
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti697S → N in CAA36998 (PubMed:1691829).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52814 Genomic DNA. Translation: CAA36998.1.
M33932 mRNA. Translation: AAA28716.1.
AE014297 Genomic DNA. Translation: AAF56942.1.
AY058596 mRNA. Translation: AAL13825.1.
X57475 Genomic DNA. Translation: CAA40713.1.
PIRiS09748.
RefSeqiNP_001287592.1. NM_001300663.1.
NP_476651.1. NM_057303.5.
UniGeneiDm.6668.

Genome annotation databases

EnsemblMetazoaiFBtr0085534; FBpp0084900; FBgn0002924.
FBtr0344976; FBpp0311231; FBgn0002924.
GeneIDi43517.
KEGGidme:Dmel_CG7831.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52814 Genomic DNA. Translation: CAA36998.1.
M33932 mRNA. Translation: AAA28716.1.
AE014297 Genomic DNA. Translation: AAF56942.1.
AY058596 mRNA. Translation: AAL13825.1.
X57475 Genomic DNA. Translation: CAA40713.1.
PIRiS09748.
RefSeqiNP_001287592.1. NM_001300663.1.
NP_476651.1. NM_057303.5.
UniGeneiDm.6668.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CZ7X-ray2.90A/B/C/D295-700[»]
1N6MX-ray2.50A/B293-700[»]
1OZXmodel-A345-667[»]
1SYJmodel-A345-667[»]
1SYPmodel-A345-667[»]
1SZ4model-A345-667[»]
1SZ5model-A345-667[»]
2NCDX-ray2.50A281-700[»]
3L1CX-ray2.75A/B293-674[»]
3U06X-ray2.35A/B293-700[»]
5HNWelectron microscopy6.60K325-348[»]
K664-700[»]
5HNXelectron microscopy6.60K325-348[»]
K664-700[»]
5HNYelectron microscopy6.30K325-348[»]
K447-493[»]
K695-700[»]
5HNZelectron microscopy5.80K325-348[»]
K430-700[»]
ProteinModelPortaliP20480.
SMRiP20480.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi68381. 17 interactors.
DIPiDIP-21302N.
IntActiP20480. 6 interactors.
MINTiMINT-949768.
STRINGi7227.FBpp0084900.

PTM databases

iPTMnetiP20480.

Proteomic databases

PaxDbiP20480.
PRIDEiP20480.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0085534; FBpp0084900; FBgn0002924.
FBtr0344976; FBpp0311231; FBgn0002924.
GeneIDi43517.
KEGGidme:Dmel_CG7831.

Organism-specific databases

CTDi43517.
FlyBaseiFBgn0002924. ncd.

Phylogenomic databases

eggNOGiKOG0239. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00550000074610.
InParanoidiP20480.
KOiK10405.
OMAiGWEYEIR.
OrthoDBiEOG091G0DJX.
PhylomeDBiP20480.

Enzyme and pathway databases

ReactomeiR-DME-983189. Kinesins.

Miscellaneous databases

EvolutionaryTraceiP20480.
GenomeRNAii43517.
PROiP20480.

Gene expression databases

BgeeiFBgn0002924.
ExpressionAtlasiP20480. baseline.
GenevisibleiP20480. DM.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 3 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNCD_DROME
AccessioniPrimary (citable) accession number: P20480
Secondary accession number(s): Q9VAG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 30, 2016
This is version 172 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.