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Protein

Glutamine synthetase 2 cytoplasmic

Gene

Gs2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glutamate-ammonia ligase activity Source: FlyBase

GO - Biological processi

  • glutamate catabolic process Source: FlyBase
  • glutamine biosynthetic process Source: FlyBase
  • neurotransmitter receptor metabolic process Source: FlyBase
  • synapse assembly Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-210455. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
R-DME-70614. Amino acid synthesis and interconversion (transamination).

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase 2 cytoplasmic (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase 2
Gene namesi
Name:Gs2
ORF Names:CG1743
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0001145. Gs2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001531481 – 369Glutamine synthetase 2 cytoplasmicAdd BLAST369

Proteomic databases

PaxDbiP20478.
PRIDEiP20478.

Expressioni

Gene expression databases

BgeeiFBgn0001145.
ExpressionAtlasiP20478. baseline.
GenevisibleiP20478. DM.

Interactioni

Subunit structurei

Homooctamer.

Protein-protein interaction databases

BioGridi58498. 1 interactor.
IntActiP20478. 26 interactors.
STRINGi7227.FBpp0073344.

Structurei

3D structure databases

ProteinModelPortaliP20478.
SMRiP20478.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiKOG0683. Eukaryota.
COG0174. LUCA.
HOGENOMiHOG000059201.
InParanoidiP20478.
KOiK01915.
OrthoDBiEOG091G07E9.
PhylomeDBiP20478.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SMARTiSM01230. Gln-synt_C. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform C (identifier: P20478-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSARILEDSP NARINKTILD RYLSLPLQEN IVQATYVWID GTGEDLRCKD
60 70 80 90 100
RTLDFIPQSP KELPVWNYDG SSCYQAEGSN SDTYLYPVAI YKDPFRRGNN
110 120 130 140 150
ILVMCDTYKF DGTPTDTNKR KTCLEVANKC AAEEPWFGIE QEYTFLDFDG
160 170 180 190 200
HPLGWPKNGF PGPQGPYYCG VGANKVYARD IVDAHYRACL YAGIKVSGTN
210 220 230 240 250
AEVMPAQWEF QVGPCEGISI GDDLWMARFL LHRISEEFGI VSTLDPKPMP
260 270 280 290 300
GDWNGAGAHT NVSTKAMRED GGIRDIEKAV AKLSKCHERH IRAYDPKQGQ
310 320 330 340 350
DNARRLTGKH ETSSINDFSA GVANRGCSIR IPRGVNDDGK GYFEDRRPSS
360
NCDPYSVVEA ILRTICLDE
Length:369
Mass (Da):41,304
Last modified:June 7, 2004 - v3
Checksum:i8B4488F01746702A
GO
Isoform A (identifier: P20478-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.

Note: No experimental confirmation available.
Show »
Length:266
Mass (Da):29,504
Checksum:i04835D0AE5FEF3E6
GO
Isoform B (identifier: P20478-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-4: SAR → HSA

Note: No experimental confirmation available.
Show »
Length:369
Mass (Da):41,285
Checksum:iF8930A3437819139
GO

Sequence cautioni

The sequence AAL28249 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti145 – 164FLDFD…FPGPQ → SDFDGHHWAGQEWFPDP in CAA36970 (PubMed:1969491).CuratedAdd BLAST20
Sequence conflicti176 – 182VYARDIV → SSPRHL in CAA36970 (PubMed:1969491).Curated7
Sequence conflicti187 – 188RA → AP in CAA36970 (PubMed:1969491).Curated2
Sequence conflicti194 – 198IKVSG → TQGVP in CAA36970 (PubMed:1969491).Curated5
Sequence conflicti222D → H in CAA36970 (PubMed:1969491).Curated1
Sequence conflicti347R → C in CAA36970 (PubMed:1969491).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0106231 – 103Missing in isoform A. CuratedAdd BLAST103
Alternative sequenceiVSP_0106242 – 4SAR → HSA in isoform B. Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52759 mRNA. Translation: CAA36970.1.
AE014298 Genomic DNA. Translation: AAF48043.2.
AE014298 Genomic DNA. Translation: AAN09632.1.
AE014298 Genomic DNA. Translation: AAS65314.1.
AY060701 mRNA. Translation: AAL28249.1. Different initiation.
PIRiS09108. AJFF2C.
RefSeqiNP_001285122.1. NM_001298193.1. [P20478-1]
NP_001285123.1. NM_001298194.1. [P20478-1]
NP_511123.2. NM_078568.3. [P20478-1]
NP_727525.1. NM_167284.2. [P20478-3]
NP_996408.1. NM_206685.2. [P20478-2]
UniGeneiDm.135.

Genome annotation databases

EnsemblMetazoaiFBtr0073495; FBpp0073345; FBgn0001145. [P20478-1]
FBtr0343562; FBpp0310163; FBgn0001145. [P20478-1]
FBtr0343563; FBpp0310164; FBgn0001145. [P20478-1]
GeneIDi32087.
KEGGidme:Dmel_CG1743.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52759 mRNA. Translation: CAA36970.1.
AE014298 Genomic DNA. Translation: AAF48043.2.
AE014298 Genomic DNA. Translation: AAN09632.1.
AE014298 Genomic DNA. Translation: AAS65314.1.
AY060701 mRNA. Translation: AAL28249.1. Different initiation.
PIRiS09108. AJFF2C.
RefSeqiNP_001285122.1. NM_001298193.1. [P20478-1]
NP_001285123.1. NM_001298194.1. [P20478-1]
NP_511123.2. NM_078568.3. [P20478-1]
NP_727525.1. NM_167284.2. [P20478-3]
NP_996408.1. NM_206685.2. [P20478-2]
UniGeneiDm.135.

3D structure databases

ProteinModelPortaliP20478.
SMRiP20478.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi58498. 1 interactor.
IntActiP20478. 26 interactors.
STRINGi7227.FBpp0073344.

Proteomic databases

PaxDbiP20478.
PRIDEiP20478.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0073495; FBpp0073345; FBgn0001145. [P20478-1]
FBtr0343562; FBpp0310163; FBgn0001145. [P20478-1]
FBtr0343563; FBpp0310164; FBgn0001145. [P20478-1]
GeneIDi32087.
KEGGidme:Dmel_CG1743.

Organism-specific databases

CTDi32087.
FlyBaseiFBgn0001145. Gs2.

Phylogenomic databases

eggNOGiKOG0683. Eukaryota.
COG0174. LUCA.
HOGENOMiHOG000059201.
InParanoidiP20478.
KOiK01915.
OrthoDBiEOG091G07E9.
PhylomeDBiP20478.

Enzyme and pathway databases

ReactomeiR-DME-210455. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
R-DME-70614. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

GenomeRNAii32087.
PROiP20478.

Gene expression databases

BgeeiFBgn0001145.
ExpressionAtlasiP20478. baseline.
GenevisibleiP20478. DM.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SMARTiSM01230. Gln-synt_C. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLNA2_DROME
AccessioniPrimary (citable) accession number: P20478
Secondary accession number(s): Q8IR90, Q95SM4, Q9VYZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: June 7, 2004
Last modified: November 30, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.