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P20478

- GLNA2_DROME

UniProt

P20478 - GLNA2_DROME

Protein

Glutamine synthetase 2 cytoplasmic

Gene

Gs2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 3 (07 Jun 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: FlyBase

    GO - Biological processi

    1. glutamate catabolic process Source: FlyBase
    2. glutamine biosynthetic process Source: FlyBase
    3. neurotransmitter receptor metabolic process Source: FlyBase
    4. synapse assembly Source: FlyBase

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_202933. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
    REACT_216833. Amino acid synthesis and interconversion (transamination).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase 2 cytoplasmic (EC:6.3.1.2)
    Alternative name(s):
    Glutamate--ammonia ligase 2
    Gene namesi
    Name:Gs2
    ORF Names:CG1743
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome X

    Organism-specific databases

    FlyBaseiFBgn0001145. Gs2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 369369Glutamine synthetase 2 cytoplasmicPRO_0000153148Add
    BLAST

    Proteomic databases

    PaxDbiP20478.

    Expressioni

    Gene expression databases

    BgeeiP20478.

    Interactioni

    Subunit structurei

    Homooctamer.

    Protein-protein interaction databases

    BioGridi58498. 1 interaction.
    STRINGi7227.FBpp0073345.

    Structurei

    3D structure databases

    ProteinModelPortaliP20478.
    SMRiP20478. Positions 15-369.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Phylogenomic databases

    eggNOGiCOG0174.
    GeneTreeiENSGT00390000010047.
    HOGENOMiHOG000059201.
    InParanoidiP20478.
    KOiK01915.
    OMAiDVEYHCK.
    OrthoDBiEOG7CZK5G.
    PhylomeDBiP20478.

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform C (identifier: P20478-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSARILEDSP NARINKTILD RYLSLPLQEN IVQATYVWID GTGEDLRCKD    50
    RTLDFIPQSP KELPVWNYDG SSCYQAEGSN SDTYLYPVAI YKDPFRRGNN 100
    ILVMCDTYKF DGTPTDTNKR KTCLEVANKC AAEEPWFGIE QEYTFLDFDG 150
    HPLGWPKNGF PGPQGPYYCG VGANKVYARD IVDAHYRACL YAGIKVSGTN 200
    AEVMPAQWEF QVGPCEGISI GDDLWMARFL LHRISEEFGI VSTLDPKPMP 250
    GDWNGAGAHT NVSTKAMRED GGIRDIEKAV AKLSKCHERH IRAYDPKQGQ 300
    DNARRLTGKH ETSSINDFSA GVANRGCSIR IPRGVNDDGK GYFEDRRPSS 350
    NCDPYSVVEA ILRTICLDE 369
    Length:369
    Mass (Da):41,304
    Last modified:June 7, 2004 - v3
    Checksum:i8B4488F01746702A
    GO
    Isoform A (identifier: P20478-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-103: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:266
    Mass (Da):29,504
    Checksum:i04835D0AE5FEF3E6
    GO
    Isoform B (identifier: P20478-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         2-4: SAR → HSA

    Note: No experimental confirmation available.

    Show »
    Length:369
    Mass (Da):41,285
    Checksum:iF8930A3437819139
    GO

    Sequence cautioni

    The sequence AAL28249.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti145 – 16420FLDFD…FPGPQ → SDFDGHHWAGQEWFPDP in CAA36970. (PubMed:1969491)CuratedAdd
    BLAST
    Sequence conflicti176 – 1827VYARDIV → SSPRHL in CAA36970. (PubMed:1969491)Curated
    Sequence conflicti187 – 1882RA → AP in CAA36970. (PubMed:1969491)Curated
    Sequence conflicti194 – 1985IKVSG → TQGVP in CAA36970. (PubMed:1969491)Curated
    Sequence conflicti222 – 2221D → H in CAA36970. (PubMed:1969491)Curated
    Sequence conflicti347 – 3471R → C in CAA36970. (PubMed:1969491)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 103103Missing in isoform A. CuratedVSP_010623Add
    BLAST
    Alternative sequencei2 – 43SAR → HSA in isoform B. CuratedVSP_010624

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52759 mRNA. Translation: CAA36970.1.
    AE014298 Genomic DNA. Translation: AAF48043.2.
    AE014298 Genomic DNA. Translation: AAN09632.1.
    AE014298 Genomic DNA. Translation: AAS65314.1.
    AY060701 mRNA. Translation: AAL28249.1. Different initiation.
    PIRiS09108. AJFF2C.
    RefSeqiNP_511123.2. NM_078568.2. [P20478-1]
    NP_727525.1. NM_167284.1. [P20478-3]
    NP_996408.1. NM_206685.1. [P20478-2]
    UniGeneiDm.135.

    Genome annotation databases

    EnsemblMetazoaiFBtr0073495; FBpp0073345; FBgn0001145. [P20478-1]
    GeneIDi32087.
    KEGGidme:Dmel_CG1743.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52759 mRNA. Translation: CAA36970.1 .
    AE014298 Genomic DNA. Translation: AAF48043.2 .
    AE014298 Genomic DNA. Translation: AAN09632.1 .
    AE014298 Genomic DNA. Translation: AAS65314.1 .
    AY060701 mRNA. Translation: AAL28249.1 . Different initiation.
    PIRi S09108. AJFF2C.
    RefSeqi NP_511123.2. NM_078568.2. [P20478-1 ]
    NP_727525.1. NM_167284.1. [P20478-3 ]
    NP_996408.1. NM_206685.1. [P20478-2 ]
    UniGenei Dm.135.

    3D structure databases

    ProteinModelPortali P20478.
    SMRi P20478. Positions 15-369.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 58498. 1 interaction.
    STRINGi 7227.FBpp0073345.

    Proteomic databases

    PaxDbi P20478.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0073495 ; FBpp0073345 ; FBgn0001145 . [P20478-1 ]
    GeneIDi 32087.
    KEGGi dme:Dmel_CG1743.

    Organism-specific databases

    CTDi 32087.
    FlyBasei FBgn0001145. Gs2.

    Phylogenomic databases

    eggNOGi COG0174.
    GeneTreei ENSGT00390000010047.
    HOGENOMi HOG000059201.
    InParanoidi P20478.
    KOi K01915.
    OMAi DVEYHCK.
    OrthoDBi EOG7CZK5G.
    PhylomeDBi P20478.

    Enzyme and pathway databases

    Reactomei REACT_202933. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
    REACT_216833. Amino acid synthesis and interconversion (transamination).

    Miscellaneous databases

    ChiTaRSi RAB27A. drosophila.
    GenomeRNAii 32087.
    NextBioi 776761.
    PROi P20478.

    Gene expression databases

    Bgeei P20478.

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Homologous nuclear genes encode cytoplasmic and mitochondrial glutamine synthetase in Drosophila melanogaster."
      Caizzi R., Bozzetti M.P., Caggese C., Ritossa F.
      J. Mol. Biol. 212:17-26(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORM C).
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 229-369.
      Strain: Berkeley.
      Tissue: Head.

    Entry informationi

    Entry nameiGLNA2_DROME
    AccessioniPrimary (citable) accession number: P20478
    Secondary accession number(s): Q8IR90, Q95SM4, Q9VYZ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: June 7, 2004
    Last modified: October 1, 2014
    This is version 116 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3