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P20478 (GLNA2_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase 2 cytoplasmic

EC=6.3.1.2
Alternative name(s):
Glutamate--ammonia ligase 2
Gene names
Name:Gs2
ORF Names:CG1743
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length369 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the glutamine synthetase family.

Sequence caution

The sequence AAL28249.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform C (identifier: P20478-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform A (identifier: P20478-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.
Note: No experimental confirmation available.
Isoform B (identifier: P20478-3)

The sequence of this isoform differs from the canonical sequence as follows:
     2-4: SAR → HSA
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 369369Glutamine synthetase 2 cytoplasmic
PRO_0000153148

Natural variations

Alternative sequence1 – 103103Missing in isoform A.
VSP_010623
Alternative sequence2 – 43SAR → HSA in isoform B.
VSP_010624

Experimental info

Sequence conflict145 – 16420FLDFD…FPGPQ → SDFDGHHWAGQEWFPDP in CAA36970. Ref.1
Sequence conflict176 – 1827VYARDIV → SSPRHL in CAA36970. Ref.1
Sequence conflict187 – 1882RA → AP in CAA36970. Ref.1
Sequence conflict194 – 1985IKVSG → TQGVP in CAA36970. Ref.1
Sequence conflict2221D → H in CAA36970. Ref.1
Sequence conflict3471R → C in CAA36970. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform C [UniParc].

Last modified June 7, 2004. Version 3.
Checksum: 8B4488F01746702A

FASTA36941,304
        10         20         30         40         50         60 
MSARILEDSP NARINKTILD RYLSLPLQEN IVQATYVWID GTGEDLRCKD RTLDFIPQSP 

        70         80         90        100        110        120 
KELPVWNYDG SSCYQAEGSN SDTYLYPVAI YKDPFRRGNN ILVMCDTYKF DGTPTDTNKR 

       130        140        150        160        170        180 
KTCLEVANKC AAEEPWFGIE QEYTFLDFDG HPLGWPKNGF PGPQGPYYCG VGANKVYARD 

       190        200        210        220        230        240 
IVDAHYRACL YAGIKVSGTN AEVMPAQWEF QVGPCEGISI GDDLWMARFL LHRISEEFGI 

       250        260        270        280        290        300 
VSTLDPKPMP GDWNGAGAHT NVSTKAMRED GGIRDIEKAV AKLSKCHERH IRAYDPKQGQ 

       310        320        330        340        350        360 
DNARRLTGKH ETSSINDFSA GVANRGCSIR IPRGVNDDGK GYFEDRRPSS NCDPYSVVEA 


ILRTICLDE 

« Hide

Isoform A [UniParc].

Checksum: 04835D0AE5FEF3E6
Show »

FASTA26629,504
Isoform B [UniParc].

Checksum: F8930A3437819139
Show »

FASTA36941,285

References

« Hide 'large scale' references
[1]"Homologous nuclear genes encode cytoplasmic and mitochondrial glutamine synthetase in Drosophila melanogaster."
Caizzi R., Bozzetti M.P., Caggese C., Ritossa F.
J. Mol. Biol. 212:17-26(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM C).
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[4]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 229-369.
Strain: Berkeley.
Tissue: Head.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X52759 mRNA. Translation: CAA36970.1.
AE014298 Genomic DNA. Translation: AAF48043.2.
AE014298 Genomic DNA. Translation: AAN09632.1.
AE014298 Genomic DNA. Translation: AAS65314.1.
AY060701 mRNA. Translation: AAL28249.1. Different initiation.
PIRAJFF2C. S09108.
RefSeqNP_511123.2. NM_078568.2. [P20478-1]
NP_727525.1. NM_167284.1. [P20478-3]
NP_996408.1. NM_206685.1. [P20478-2]
UniGeneDm.135.

3D structure databases

ProteinModelPortalP20478.
SMRP20478. Positions 15-369.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid58498. 1 interaction.
STRING7227.FBpp0073345.

Proteomic databases

PaxDbP20478.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0073495; FBpp0073345; FBgn0001145. [P20478-1]
GeneID32087.
KEGGdme:Dmel_CG1743.

Organism-specific databases

CTD32087.
FlyBaseFBgn0001145. Gs2.

Phylogenomic databases

eggNOGCOG0174.
GeneTreeENSGT00390000010047.
HOGENOMHOG000059201.
InParanoidP20478.
KOK01915.
OMADVEYHCK.
OrthoDBEOG7CZK5G.
PhylomeDBP20478.

Gene expression databases

BgeeP20478.

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRAB27A. drosophila.
GenomeRNAi32087.
NextBio776761.
PROP20478.

Entry information

Entry nameGLNA2_DROME
AccessionPrimary (citable) accession number: P20478
Secondary accession number(s): Q8IR90, Q95SM4, Q9VYZ0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: June 7, 2004
Last modified: July 9, 2014
This is version 114 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase