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P20478

- GLNA2_DROME

UniProt

P20478 - GLNA2_DROME

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Protein

Glutamine synthetase 2 cytoplasmic

Gene

Gs2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: FlyBase

GO - Biological processi

  1. glutamate catabolic process Source: FlyBase
  2. glutamine biosynthetic process Source: FlyBase
  3. neurotransmitter receptor metabolic process Source: FlyBase
  4. synapse assembly Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_202933. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
REACT_216833. Amino acid synthesis and interconversion (transamination).

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase 2 cytoplasmic (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase 2
Gene namesi
Name:Gs2
ORF Names:CG1743
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome X

Organism-specific databases

FlyBaseiFBgn0001145. Gs2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Glutamine synthetase 2 cytoplasmicPRO_0000153148Add
BLAST

Proteomic databases

PaxDbiP20478.

Expressioni

Gene expression databases

BgeeiP20478.
ExpressionAtlasiP20478. differential.

Interactioni

Subunit structurei

Homooctamer.

Protein-protein interaction databases

BioGridi58498. 1 interaction.
STRINGi7227.FBpp0073345.

Structurei

3D structure databases

ProteinModelPortaliP20478.
SMRiP20478. Positions 15-369.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiCOG0174.
GeneTreeiENSGT00390000010047.
HOGENOMiHOG000059201.
InParanoidiP20478.
KOiK01915.
OMAiDVEYHCK.
OrthoDBiEOG7CZK5G.
PhylomeDBiP20478.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform C (identifier: P20478-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSARILEDSP NARINKTILD RYLSLPLQEN IVQATYVWID GTGEDLRCKD
60 70 80 90 100
RTLDFIPQSP KELPVWNYDG SSCYQAEGSN SDTYLYPVAI YKDPFRRGNN
110 120 130 140 150
ILVMCDTYKF DGTPTDTNKR KTCLEVANKC AAEEPWFGIE QEYTFLDFDG
160 170 180 190 200
HPLGWPKNGF PGPQGPYYCG VGANKVYARD IVDAHYRACL YAGIKVSGTN
210 220 230 240 250
AEVMPAQWEF QVGPCEGISI GDDLWMARFL LHRISEEFGI VSTLDPKPMP
260 270 280 290 300
GDWNGAGAHT NVSTKAMRED GGIRDIEKAV AKLSKCHERH IRAYDPKQGQ
310 320 330 340 350
DNARRLTGKH ETSSINDFSA GVANRGCSIR IPRGVNDDGK GYFEDRRPSS
360
NCDPYSVVEA ILRTICLDE
Length:369
Mass (Da):41,304
Last modified:June 7, 2004 - v3
Checksum:i8B4488F01746702A
GO
Isoform A (identifier: P20478-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.

Note: No experimental confirmation available.

Show »
Length:266
Mass (Da):29,504
Checksum:i04835D0AE5FEF3E6
GO
Isoform B (identifier: P20478-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-4: SAR → HSA

Note: No experimental confirmation available.

Show »
Length:369
Mass (Da):41,285
Checksum:iF8930A3437819139
GO

Sequence cautioni

The sequence AAL28249.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti145 – 16420FLDFD…FPGPQ → SDFDGHHWAGQEWFPDP in CAA36970. (PubMed:1969491)CuratedAdd
BLAST
Sequence conflicti176 – 1827VYARDIV → SSPRHL in CAA36970. (PubMed:1969491)Curated
Sequence conflicti187 – 1882RA → AP in CAA36970. (PubMed:1969491)Curated
Sequence conflicti194 – 1985IKVSG → TQGVP in CAA36970. (PubMed:1969491)Curated
Sequence conflicti222 – 2221D → H in CAA36970. (PubMed:1969491)Curated
Sequence conflicti347 – 3471R → C in CAA36970. (PubMed:1969491)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 103103Missing in isoform A. CuratedVSP_010623Add
BLAST
Alternative sequencei2 – 43SAR → HSA in isoform B. CuratedVSP_010624

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52759 mRNA. Translation: CAA36970.1.
AE014298 Genomic DNA. Translation: AAF48043.2.
AE014298 Genomic DNA. Translation: AAN09632.1.
AE014298 Genomic DNA. Translation: AAS65314.1.
AY060701 mRNA. Translation: AAL28249.1. Different initiation.
PIRiS09108. AJFF2C.
RefSeqiNP_001285122.1. NM_001298193.1. [P20478-1]
NP_001285123.1. NM_001298194.1. [P20478-1]
NP_511123.2. NM_078568.3. [P20478-1]
NP_727525.1. NM_167284.2. [P20478-3]
NP_996408.1. NM_206685.2. [P20478-2]
UniGeneiDm.135.

Genome annotation databases

EnsemblMetazoaiFBtr0073495; FBpp0073345; FBgn0001145. [P20478-1]
GeneIDi32087.
KEGGidme:Dmel_CG1743.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52759 mRNA. Translation: CAA36970.1 .
AE014298 Genomic DNA. Translation: AAF48043.2 .
AE014298 Genomic DNA. Translation: AAN09632.1 .
AE014298 Genomic DNA. Translation: AAS65314.1 .
AY060701 mRNA. Translation: AAL28249.1 . Different initiation.
PIRi S09108. AJFF2C.
RefSeqi NP_001285122.1. NM_001298193.1. [P20478-1 ]
NP_001285123.1. NM_001298194.1. [P20478-1 ]
NP_511123.2. NM_078568.3. [P20478-1 ]
NP_727525.1. NM_167284.2. [P20478-3 ]
NP_996408.1. NM_206685.2. [P20478-2 ]
UniGenei Dm.135.

3D structure databases

ProteinModelPortali P20478.
SMRi P20478. Positions 15-369.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 58498. 1 interaction.
STRINGi 7227.FBpp0073345.

Proteomic databases

PaxDbi P20478.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0073495 ; FBpp0073345 ; FBgn0001145 . [P20478-1 ]
GeneIDi 32087.
KEGGi dme:Dmel_CG1743.

Organism-specific databases

CTDi 32087.
FlyBasei FBgn0001145. Gs2.

Phylogenomic databases

eggNOGi COG0174.
GeneTreei ENSGT00390000010047.
HOGENOMi HOG000059201.
InParanoidi P20478.
KOi K01915.
OMAi DVEYHCK.
OrthoDBi EOG7CZK5G.
PhylomeDBi P20478.

Enzyme and pathway databases

Reactomei REACT_202933. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
REACT_216833. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

GenomeRNAii 32087.
NextBioi 776761.
PROi P20478.

Gene expression databases

Bgeei P20478.
ExpressionAtlasi P20478. differential.

Family and domain databases

Gene3Di 3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
PROSITEi PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Homologous nuclear genes encode cytoplasmic and mitochondrial glutamine synthetase in Drosophila melanogaster."
    Caizzi R., Bozzetti M.P., Caggese C., Ritossa F.
    J. Mol. Biol. 212:17-26(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM C).
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 229-369.
    Strain: Berkeley.
    Tissue: Head.

Entry informationi

Entry nameiGLNA2_DROME
AccessioniPrimary (citable) accession number: P20478
Secondary accession number(s): Q8IR90, Q95SM4, Q9VYZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: June 7, 2004
Last modified: November 26, 2014
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3