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P20478

- GLNA2_DROME

UniProt

P20478 - GLNA2_DROME

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Protein
Glutamine synthetase 2 cytoplasmic
Gene
Gs2, CG1743
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: FlyBase

GO - Biological processi

  1. glutamate catabolic process Source: FlyBase
  2. glutamine biosynthetic process Source: FlyBase
  3. neurotransmitter receptor metabolic process Source: FlyBase
  4. synapse assembly Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_202933. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
REACT_216833. Amino acid synthesis and interconversion (transamination).

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase 2 cytoplasmic (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase 2
Gene namesi
Name:Gs2
ORF Names:CG1743
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome X

Organism-specific databases

FlyBaseiFBgn0001145. Gs2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Glutamine synthetase 2 cytoplasmic
PRO_0000153148Add
BLAST

Proteomic databases

PaxDbiP20478.

Expressioni

Gene expression databases

BgeeiP20478.

Interactioni

Subunit structurei

Homooctamer.

Protein-protein interaction databases

BioGridi58498. 1 interaction.
STRINGi7227.FBpp0073345.

Structurei

3D structure databases

ProteinModelPortaliP20478.
SMRiP20478. Positions 15-369.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0174.
GeneTreeiENSGT00390000010047.
HOGENOMiHOG000059201.
InParanoidiP20478.
KOiK01915.
OMAiDVEYHCK.
OrthoDBiEOG7CZK5G.
PhylomeDBiP20478.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform C (identifier: P20478-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSARILEDSP NARINKTILD RYLSLPLQEN IVQATYVWID GTGEDLRCKD    50
RTLDFIPQSP KELPVWNYDG SSCYQAEGSN SDTYLYPVAI YKDPFRRGNN 100
ILVMCDTYKF DGTPTDTNKR KTCLEVANKC AAEEPWFGIE QEYTFLDFDG 150
HPLGWPKNGF PGPQGPYYCG VGANKVYARD IVDAHYRACL YAGIKVSGTN 200
AEVMPAQWEF QVGPCEGISI GDDLWMARFL LHRISEEFGI VSTLDPKPMP 250
GDWNGAGAHT NVSTKAMRED GGIRDIEKAV AKLSKCHERH IRAYDPKQGQ 300
DNARRLTGKH ETSSINDFSA GVANRGCSIR IPRGVNDDGK GYFEDRRPSS 350
NCDPYSVVEA ILRTICLDE 369
Length:369
Mass (Da):41,304
Last modified:June 7, 2004 - v3
Checksum:i8B4488F01746702A
GO
Isoform A (identifier: P20478-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.

Note: No experimental confirmation available.

Show »
Length:266
Mass (Da):29,504
Checksum:i04835D0AE5FEF3E6
GO
Isoform B (identifier: P20478-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-4: SAR → HSA

Note: No experimental confirmation available.

Show »
Length:369
Mass (Da):41,285
Checksum:iF8930A3437819139
GO

Sequence cautioni

The sequence AAL28249.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 103103Missing in isoform A.
VSP_010623Add
BLAST
Alternative sequencei2 – 43SAR → HSA in isoform B.
VSP_010624

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti145 – 16420FLDFD…FPGPQ → SDFDGHHWAGQEWFPDP in CAA36970. 1 Publication
Add
BLAST
Sequence conflicti176 – 1827VYARDIV → SSPRHL in CAA36970. 1 Publication
Sequence conflicti187 – 1882RA → AP in CAA36970. 1 Publication
Sequence conflicti194 – 1985IKVSG → TQGVP in CAA36970. 1 Publication
Sequence conflicti222 – 2221D → H in CAA36970. 1 Publication
Sequence conflicti347 – 3471R → C in CAA36970. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52759 mRNA. Translation: CAA36970.1.
AE014298 Genomic DNA. Translation: AAF48043.2.
AE014298 Genomic DNA. Translation: AAN09632.1.
AE014298 Genomic DNA. Translation: AAS65314.1.
AY060701 mRNA. Translation: AAL28249.1. Different initiation.
PIRiS09108. AJFF2C.
RefSeqiNP_511123.2. NM_078568.2. [P20478-1]
NP_727525.1. NM_167284.1. [P20478-3]
NP_996408.1. NM_206685.1. [P20478-2]
UniGeneiDm.135.

Genome annotation databases

EnsemblMetazoaiFBtr0073495; FBpp0073345; FBgn0001145. [P20478-1]
GeneIDi32087.
KEGGidme:Dmel_CG1743.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52759 mRNA. Translation: CAA36970.1 .
AE014298 Genomic DNA. Translation: AAF48043.2 .
AE014298 Genomic DNA. Translation: AAN09632.1 .
AE014298 Genomic DNA. Translation: AAS65314.1 .
AY060701 mRNA. Translation: AAL28249.1 . Different initiation.
PIRi S09108. AJFF2C.
RefSeqi NP_511123.2. NM_078568.2. [P20478-1 ]
NP_727525.1. NM_167284.1. [P20478-3 ]
NP_996408.1. NM_206685.1. [P20478-2 ]
UniGenei Dm.135.

3D structure databases

ProteinModelPortali P20478.
SMRi P20478. Positions 15-369.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 58498. 1 interaction.
STRINGi 7227.FBpp0073345.

Proteomic databases

PaxDbi P20478.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0073495 ; FBpp0073345 ; FBgn0001145 . [P20478-1 ]
GeneIDi 32087.
KEGGi dme:Dmel_CG1743.

Organism-specific databases

CTDi 32087.
FlyBasei FBgn0001145. Gs2.

Phylogenomic databases

eggNOGi COG0174.
GeneTreei ENSGT00390000010047.
HOGENOMi HOG000059201.
InParanoidi P20478.
KOi K01915.
OMAi DVEYHCK.
OrthoDBi EOG7CZK5G.
PhylomeDBi P20478.

Enzyme and pathway databases

Reactomei REACT_202933. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
REACT_216833. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

ChiTaRSi RAB27A. drosophila.
GenomeRNAii 32087.
NextBioi 776761.
PROi P20478.

Gene expression databases

Bgeei P20478.

Family and domain databases

Gene3Di 3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
PROSITEi PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Homologous nuclear genes encode cytoplasmic and mitochondrial glutamine synthetase in Drosophila melanogaster."
    Caizzi R., Bozzetti M.P., Caggese C., Ritossa F.
    J. Mol. Biol. 212:17-26(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM C).
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 229-369.
    Strain: Berkeley.
    Tissue: Head.

Entry informationi

Entry nameiGLNA2_DROME
AccessioniPrimary (citable) accession number: P20478
Secondary accession number(s): Q8IR90, Q95SM4, Q9VYZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: June 7, 2004
Last modified: September 3, 2014
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi