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Protein

Glutamine synthetase 2 cytoplasmic

Gene

Gs2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Caution

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glutamate-ammonia ligase activity Source: FlyBase

GO - Biological processi

  • glutamate catabolic process Source: FlyBase
  • glutamine biosynthetic process Source: FlyBase
  • neurotransmitter receptor metabolic process Source: FlyBase
  • synapse assembly Source: FlyBase

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-210455 Astrocytic Glutamate-Glutamine Uptake And Metabolism
R-DME-70614 Amino acid synthesis and interconversion (transamination)

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase 2 cytoplasmic (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase 2
Gene namesi
Name:Gs2
ORF Names:CG1743
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0001145 Gs2

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001531481 – 369Glutamine synthetase 2 cytoplasmicAdd BLAST369

Proteomic databases

PaxDbiP20478
PRIDEiP20478

Expressioni

Gene expression databases

BgeeiFBgn0001145
ExpressionAtlasiP20478 differential
GenevisibleiP20478 DM

Interactioni

Subunit structurei

Homooctamer.

Protein-protein interaction databases

BioGridi584981 interactor.
IntActiP20478 28 interactors.
STRINGi7227.FBpp0073344

Structurei

3D structure databases

ProteinModelPortaliP20478
SMRiP20478
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiKOG0683 Eukaryota
COG0174 LUCA
GeneTreeiENSGT00390000010047
HOGENOMiHOG000059201
InParanoidiP20478
KOiK01915
OMAiMMPDGVT
OrthoDBiEOG091G07E9
PhylomeDBiP20478

Family and domain databases

Gene3Di3.10.20.701 hit
InterProiView protein in InterPro
IPR008147 Gln_synt_b-grasp
IPR036651 Gln_synt_N
IPR014746 Gln_synth/guanido_kin_cat_dom
IPR008146 Gln_synth_cat_dom
IPR027303 Gln_synth_gly_rich_site
IPR027302 Gln_synth_N_conserv_site
PfamiView protein in Pfam
PF00120 Gln-synt_C, 1 hit
PF03951 Gln-synt_N, 1 hit
SMARTiView protein in SMART
SM01230 Gln-synt_C, 1 hit
SUPFAMiSSF54368 SSF54368, 1 hit
SSF55931 SSF55931, 1 hit
PROSITEiView protein in PROSITE
PS00180 GLNA_1, 1 hit
PS00181 GLNA_ATP, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform C (identifier: P20478-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSARILEDSP NARINKTILD RYLSLPLQEN IVQATYVWID GTGEDLRCKD
60 70 80 90 100
RTLDFIPQSP KELPVWNYDG SSCYQAEGSN SDTYLYPVAI YKDPFRRGNN
110 120 130 140 150
ILVMCDTYKF DGTPTDTNKR KTCLEVANKC AAEEPWFGIE QEYTFLDFDG
160 170 180 190 200
HPLGWPKNGF PGPQGPYYCG VGANKVYARD IVDAHYRACL YAGIKVSGTN
210 220 230 240 250
AEVMPAQWEF QVGPCEGISI GDDLWMARFL LHRISEEFGI VSTLDPKPMP
260 270 280 290 300
GDWNGAGAHT NVSTKAMRED GGIRDIEKAV AKLSKCHERH IRAYDPKQGQ
310 320 330 340 350
DNARRLTGKH ETSSINDFSA GVANRGCSIR IPRGVNDDGK GYFEDRRPSS
360
NCDPYSVVEA ILRTICLDE
Length:369
Mass (Da):41,304
Last modified:June 7, 2004 - v3
Checksum:i8B4488F01746702A
GO
Isoform A (identifier: P20478-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.

Note: No experimental confirmation available.
Show »
Length:266
Mass (Da):29,504
Checksum:i04835D0AE5FEF3E6
GO
Isoform B (identifier: P20478-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-4: SAR → HSA

Note: No experimental confirmation available.
Show »
Length:369
Mass (Da):41,285
Checksum:iF8930A3437819139
GO

Sequence cautioni

The sequence AAL28249 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti145 – 164FLDFD…FPGPQ → SDFDGHHWAGQEWFPDP in CAA36970 (PubMed:1969491).CuratedAdd BLAST20
Sequence conflicti176 – 182VYARDIV → SSPRHL in CAA36970 (PubMed:1969491).Curated7
Sequence conflicti187 – 188RA → AP in CAA36970 (PubMed:1969491).Curated2
Sequence conflicti194 – 198IKVSG → TQGVP in CAA36970 (PubMed:1969491).Curated5
Sequence conflicti222D → H in CAA36970 (PubMed:1969491).Curated1
Sequence conflicti347R → C in CAA36970 (PubMed:1969491).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0106231 – 103Missing in isoform A. CuratedAdd BLAST103
Alternative sequenceiVSP_0106242 – 4SAR → HSA in isoform B. Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52759 mRNA Translation: CAA36970.1
AE014298 Genomic DNA Translation: AAF48043.2
AE014298 Genomic DNA Translation: AAN09632.1
AE014298 Genomic DNA Translation: AAS65314.1
AY060701 mRNA Translation: AAL28249.1 Different initiation.
PIRiS09108 AJFF2C
RefSeqiNP_001285122.1, NM_001298193.1 [P20478-1]
NP_001285123.1, NM_001298194.1 [P20478-1]
NP_511123.2, NM_078568.3 [P20478-1]
NP_727525.1, NM_167284.2 [P20478-3]
NP_996408.1, NM_206685.2 [P20478-2]
UniGeneiDm.135

Genome annotation databases

EnsemblMetazoaiFBtr0073494; FBpp0073344; FBgn0001145 [P20478-3]
FBtr0073495; FBpp0073345; FBgn0001145 [P20478-1]
FBtr0073496; FBpp0089332; FBgn0001145 [P20478-2]
FBtr0343561; FBpp0310162; FBgn0001145
FBtr0343562; FBpp0310163; FBgn0001145 [P20478-1]
FBtr0343563; FBpp0310164; FBgn0001145 [P20478-1]
GeneIDi32087
KEGGidme:Dmel_CG1743

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGLNA2_DROME
AccessioniPrimary (citable) accession number: P20478
Secondary accession number(s): Q8IR90, Q95SM4, Q9VYZ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: June 7, 2004
Last modified: March 28, 2018
This is version 145 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome