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P20477 (GLNA1_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase 1, mitochondrial

EC=6.3.1.2
Alternative name(s):
Glutamate--ammonia ligase 1
Gene names
Name:Gs1
ORF Names:CG2718
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length399 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer.

Subcellular location

Mitochondrion.

Sequence similarities

Belongs to the glutamine synthetase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2727Mitochondrion Potential
Chain28 – 399372Glutamine synthetase 1, mitochondrial
PRO_0000011173

Experimental info

Sequence conflict21A → R in CAA10031. Ref.2
Sequence conflict178 – 1836DVDGRP → RRGRTS in CAA36971. Ref.1
Sequence conflict2881A → R in CAA36971. Ref.1
Sequence conflict3091A → P in CAA36971. Ref.1
Sequence conflict3251D → Y in CAA36971. Ref.1
Sequence conflict3931R → Q in CAA36971. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P20477 [UniParc].

Last modified June 21, 2005. Version 3.
Checksum: 935E8D1D9927ACCC

FASTA39944,396
        10         20         30         40         50         60 
MALRVAGLFL KKELVAPATQ QLRLLRTGNT TRSQFLANSP NTALDKSILQ RYRNLETPAN 

        70         80         90        100        110        120 
RVQATYLWID GTGENIRLKD RVLDKVPSSV EDLPDWQYDG SSTYQAHGEN SDTTLKPRAI 

       130        140        150        160        170        180 
YRDPFKPGKN DVIVLCDTYS ADGKPTASNK RAAFQAAIDL ISDQEPWFGI EQEYTLLDVD 

       190        200        210        220        230        240 
GRPFGWPENG FPAPQGPYYC GVGADRVYAR DLVEAHVVAC LYAGIDFAGT NAEVMPAQWE 

       250        260        270        280        290        300 
FQIGPAGIKA CDDLWVSRYI LQRIAEEYGV VVTFDPKPME GQWNGAGAHT NFSTKEMRAD 

       310        320        330        340        350        360 
GGIKAIEEAI EKLSKRHERH IKAYDPKEGK DNERRLVGRL ETSSIDKFSW GVANRAVSVR 

       370        380        390 
VPRGVATAGK GYLEDRRPSS NCDPYAVCNA IVRTCLLNE 

« Hide

References

« Hide 'large scale' references
[1]"Homologous nuclear genes encode cytoplasmic and mitochondrial glutamine synthetase in Drosophila melanogaster."
Caizzi R., Bozzetti M.P., Caggese C., Ritossa F.
J. Mol. Biol. 212:17-26(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Glover D.M.
Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[4]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[5]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X52760 mRNA. Translation: CAA36971.1.
AJ012460 mRNA. Translation: CAA10031.1.
AE014134 Genomic DNA. Translation: AAF51546.1.
AE014134 Genomic DNA. Translation: AAF51547.1.
AY058730 mRNA. Translation: AAL13959.1.
PIRAJFF1M. S09109.
RefSeqNP_001162839.1. NM_001169368.2.
NP_476570.1. NM_057222.3.
NP_722606.1. NM_164367.1.
UniGeneDm.6999.

3D structure databases

ProteinModelPortalP20477.
SMRP20477. Positions 45-399.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid59433. 20 interactions.
MINTMINT-297766.

Proteomic databases

PaxDbP20477.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0078114; FBpp0077773; FBgn0001142.
FBtr0078115; FBpp0077774; FBgn0001142.
FBtr0300568; FBpp0289795; FBgn0001142.
GeneID33172.
KEGGdme:Dmel_CG2718.

Organism-specific databases

CTD33172.
FlyBaseFBgn0001142. Gs1.

Phylogenomic databases

eggNOGCOG0174.
GeneTreeENSGT00390000010047.
HOGENOMHOG000246596.
InParanoidP20477.
KOK01915.
OMAKSFGRDV.
OrthoDBEOG7CZK5G.
PhylomeDBP20477.

Gene expression databases

BgeeP20477.

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi33172.
NextBio782262.

Entry information

Entry nameGLNA1_DROME
AccessionPrimary (citable) accession number: P20477
Secondary accession number(s): A4UZX3, O96770, Q9VPK0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: June 21, 2005
Last modified: July 9, 2014
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase