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Protein

Glutamine synthetase 1, mitochondrial

Gene

Gs1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glutamate-ammonia ligase activity Source: FlyBase

GO - Biological processi

  • glutamine biosynthetic process Source: InterPro
  • glutamine metabolic process Source: FlyBase
  • mitochondrion organization Source: FlyBase
  • phagocytosis Source: FlyBase

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-210455. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
R-DME-70614. Amino acid synthesis and interconversion (transamination).

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase 1, mitochondrial (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase 1
Gene namesi
Name:Gs1
ORF Names:CG2718
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0001142. Gs1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 27MitochondrionSequence analysisAdd BLAST27
ChainiPRO_000001117328 – 399Glutamine synthetase 1, mitochondrialAdd BLAST372

Proteomic databases

PaxDbiP20477.
PRIDEiP20477.

Expressioni

Gene expression databases

BgeeiFBgn0001142.
ExpressionAtlasiP20477. differential.
GenevisibleiP20477. DM.

Interactioni

Subunit structurei

Homooctamer.

Protein-protein interaction databases

BioGridi59433. 20 interactors.
MINTiMINT-297766.
STRINGi7227.FBpp0077773.

Structurei

3D structure databases

ProteinModelPortaliP20477.
SMRiP20477.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0683. Eukaryota.
COG0174. LUCA.
GeneTreeiENSGT00390000010047.
HOGENOMiHOG000246596.
InParanoidiP20477.
KOiK01915.
OMAiWPENGFP.
OrthoDBiEOG091G07E9.
PhylomeDBiP20477.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR036651. Gln_synt_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
SSF55931. SSF55931. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20477-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALRVAGLFL KKELVAPATQ QLRLLRTGNT TRSQFLANSP NTALDKSILQ
60 70 80 90 100
RYRNLETPAN RVQATYLWID GTGENIRLKD RVLDKVPSSV EDLPDWQYDG
110 120 130 140 150
SSTYQAHGEN SDTTLKPRAI YRDPFKPGKN DVIVLCDTYS ADGKPTASNK
160 170 180 190 200
RAAFQAAIDL ISDQEPWFGI EQEYTLLDVD GRPFGWPENG FPAPQGPYYC
210 220 230 240 250
GVGADRVYAR DLVEAHVVAC LYAGIDFAGT NAEVMPAQWE FQIGPAGIKA
260 270 280 290 300
CDDLWVSRYI LQRIAEEYGV VVTFDPKPME GQWNGAGAHT NFSTKEMRAD
310 320 330 340 350
GGIKAIEEAI EKLSKRHERH IKAYDPKEGK DNERRLVGRL ETSSIDKFSW
360 370 380 390
GVANRAVSVR VPRGVATAGK GYLEDRRPSS NCDPYAVCNA IVRTCLLNE
Length:399
Mass (Da):44,396
Last modified:June 21, 2005 - v3
Checksum:i935E8D1D9927ACCC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2A → R in CAA10031 (Ref. 2) Curated1
Sequence conflicti178 – 183DVDGRP → RRGRTS in CAA36971 (PubMed:1969491).Curated6
Sequence conflicti288A → R in CAA36971 (PubMed:1969491).Curated1
Sequence conflicti309A → P in CAA36971 (PubMed:1969491).Curated1
Sequence conflicti325D → Y in CAA36971 (PubMed:1969491).Curated1
Sequence conflicti393R → Q in CAA36971 (PubMed:1969491).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52760 mRNA. Translation: CAA36971.1.
AJ012460 mRNA. Translation: CAA10031.1.
AE014134 Genomic DNA. Translation: AAF51546.1.
AE014134 Genomic DNA. Translation: AAF51547.1.
AY058730 mRNA. Translation: AAL13959.1.
PIRiS09109. AJFF1M.
RefSeqiNP_001162839.1. NM_001169368.3.
NP_476570.1. NM_057222.4.
NP_722606.1. NM_164367.2.
UniGeneiDm.6999.

Genome annotation databases

EnsemblMetazoaiFBtr0078114; FBpp0077773; FBgn0001142.
FBtr0078115; FBpp0077774; FBgn0001142.
FBtr0300568; FBpp0289795; FBgn0001142.
GeneIDi33172.
KEGGidme:Dmel_CG2718.

Similar proteinsi

Entry informationi

Entry nameiGLNA1_DROME
AccessioniPrimary (citable) accession number: P20477
Secondary accession number(s): A4UZX3, O96770, Q9VPK0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: June 21, 2005
Last modified: October 25, 2017
This is version 138 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families