P20472 (PRVA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Parvalbumin alpha

Gene names

Name:

PVALB

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	110 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.
Sequence similarities	Belongs to the parvalbumin family. Contains 2 EF-hand domains.
Mass spectrometry	Molecular mass is 11927±0.76 Da from positions 2 - 110. Determined by ESI. Ref.8

Ontologies

Keywords
Domain	Repeat
Ligand	Calcium Metal-binding
Molecular function	Muscle protein
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	axon Inferred from electronic annotation. Source: Compara cytoplasm Inferred from electronic annotation. Source: Compara
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.2

Chain

2 – 110

109

Parvalbumin alpha

PRO_0000073588

Regions

Domain

39 – 74

36

EF-hand 1

Domain

78 – 110

33

EF-hand 2

Calcium binding

52 – 63

12

1

Calcium binding

91 – 102

12

2

Amino acid modifications

Modified residue

2

1

N-acetylserine Ref.8

Modified residue

79

1

Phosphoserine By similarity

Modified residue

83

1

Phosphothreonine By similarity

Experimental info

Mutagenesis

52

1

D → A: Inactivation.

Mutagenesis

63

1

E → V: Inactivation.

Mutagenesis

91

1

D → A: Inactivation.

Mutagenesis

102

1

E → V: Inactivation.

Sequence conflict

4

1

T → N in AAH96113. Ref.7

Sequence conflict

99 – 100

2

Missing in CAA45134. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

110

Helix Strand Turn

Details...

Turn

3 – 8

6

Helix

9 – 18

10

Beta strand

21 – 23

3

Helix

27 – 33

7

Helix

36 – 38

3

Helix

41 – 51

11

Helix

61 – 64

4

Turn

65 – 67

3

Helix

68 – 71

4

Helix

80 – 90

11

Beta strand

92 – 98

7

Helix

100 – 106

7

Turn

107 – 109

3

Sequences

	Sequence		Length	Mass (Da)
	P20472 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 03ABDD22E24AEA11	FASTA	110	12,059
	10 20 30 40 50 60 MSMTDLLNAE DIKKAVGAFS ATDSFDHKKF FQMVGLKKKS ADDVKKVFHM LDKDKSGFIE 70 80 90 100 110 EDELGFILKG FSPDARDLSA KETKMLMAAG DKDGDGKIGV DEFSTLVAES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Parvalbumin genes from human and rat are identical in intron/exon organization and contain highly homologous regulatory elements and coding sequences." Berchtold M.W. J. Mol. Biol. 210:417-427(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Human alpha and beta parvalbumins. Structure and tissue-specific expression." Foehr U.G., Weber B.R., Muentener M., Staudenmann W., Hughes G.J., Frutiger S., Banville D., Schaefer B.W., Heizmann C.W. Eur. J. Biochem. 215:719-727(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-110. Tissue: Cerebellum.
[3]	"A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cerebellum.
[5]	"The DNA sequence of human chromosome 22." Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H. Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]	"Electrospray ionization mass spectrometry: analysis of the Ca2+-binding properties of human recombinant alpha-parvalbumin and nine mutant proteins." Troxler H., Kuster T., Rhyner J.A., Gehrig P., Heizmann C.W. Anal. Biochem. 268:64-71(1999) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS, ACETYLATION AT SER-2, MASS SPECTROMETRY.
[9]	"Paramagnetism-based refinement strategy for the solution structure of human alpha-parvalbumin." Baig I., Bertini I., Del Bianco C., Gupta Y.K., Lee Y.M., Luchinat C., Quattrone A. Biochemistry 43:5562-5573(2004) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X63578 Genomic DNA. Translation: CAA45134.1.
X52695 , X52696, X52697, X52698 Genomic DNA. Translation: CAA36924.1.
X63070 mRNA. Translation: CAA44792.1.
CR456552 mRNA. Translation: CAG30438.1.
AK311832 mRNA. Translation: BAG34774.1.
Z82184, Z82185 Genomic DNA. Translation: CAI23584.1.
Z82185, Z82184 Genomic DNA. Translation: CAI23585.1.
CH471095 Genomic DNA. Translation: EAW60118.1.
BC069300 mRNA. Translation: AAH69300.1.
BC096112 mRNA. Translation: AAH96112.1.
BC096113 mRNA. Translation: AAH96113.1.
BC096114 mRNA. Translation: AAH96114.1.
BC096115 mRNA. Translation: AAH96115.1.

IPI

IPI00219703.

PIR

S07531.

RefSeq

NP_002845.1. NM_002854.2.

UniGene

Hs.295449.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RJV	NMR	-	A	1-110	[»]
1RK9	NMR	-	A	2-109	[»]

ProteinModelPortal

P20472.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000216200.

PTM databases

PhosphoSite

P20472.

Polymorphism databases

DMDM

131100.

Proteomic databases

PaxDb

P20472.

PRIDE

P20472.

Protocols and materials databases

DNASU

5816.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000216200; ENSP00000216200; ENSG00000100362.
ENST00000417718; ENSP00000400247; ENSG00000100362.

GeneID

5816.

KEGG

hsa:5816.

UCSC

uc003apx.3. human.

Organism-specific databases

CTD

5816.

GeneCards

GC22M037196.

HGNC

HGNC:9704. PVALB.

HPA

CAB002515.

MIM

168890. gene.

neXtProt

NX_P20472.

PharmGKB

PA34049.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG278774.

HOGENOM

HOG000037438.

HOVERGEN

HBG107490.

InParanoid

P20472.

OMA

AVDSFDH.

OrthoDB

EOG4BK55C.

PhylomeDB

P20472.

Gene expression databases

ArrayExpress

P20472.

Bgee

P20472.

CleanEx

HS_PVALB.

Genevestigator

P20472.

GermOnline

ENSG00000100362. Homo sapiens.

Family and domain databases

Gene3D

1.10.238.10. 1 hit.

InterPro

IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR008080. Parvalbumin.
[Graphical view]

PANTHER

PTHR11653. PTHR11653. 1 hit.

Pfam

PF13499. EF_hand_5. 1 hit.
[Graphical view]

SMART

SM00054. EFh. 2 hits.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

PVALB. human.

EvolutionaryTrace

P20472.

GenomeRNAi

5816.

NextBio

22650.

SOURCE

Search...

Entry information

Entry name

PRVA_HUMAN

Accession

Primary (citable) accession number: P20472
Secondary accession number(s): B2R4H7 , P78378, Q4VB78, Q5R3Q9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 130 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.