P20470 (L_BUNYW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L
EC=2.7.7.48

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Gene names

Name:

L

Organism

Bunyamwera virus (BUNV) [Reference proteome]

Taxonomic identifier

35304 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Bunyaviridae › Orthobunyavirus

Virus host

Aedes aegypti (Yellowfever mosquito) (Culex aegypti) [TaxID: 7159]
Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	2238 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Virion Potential.
Sequence similarities	Belongs to the orthobunyavirus RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway Viral RNA replication
Cellular component	Virion
Ligand	Nucleotide-binding
Molecular function	Hydrolase Nucleotidyltransferase Protease RNA-directed RNA polymerase Thiol protease Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular_component	virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2238	2238	RNA-directed RNA polymerase L		PRO_0000222020
Regions
	Domain	1016 – 1207	192	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	P20470 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 1ED00AB156BAC8DA	FASTA	2,238	258,670
	10 20 30 40 50 60 MEDQAYDQYL HRIQAARTAT VAKDISADIL EARHDYFGRE LCNSLGIEYK NNVLLDEIIL 70 80 90 100 110 120 DVVPGVNLLN YNIPNVTPDN YIWDGHFLII LDYKVSVGND SSEITYKKYT SLILPVMSEL 130 140 150 160 170 180 GIDTEIAIIR ANPVTYQISI IGEEFKQRFP NIPIQLDFGR FFELRKMLLD KFADDEEFLM 190 200 210 220 230 240 MIAHGDFTLT APWCTSDTPE LEEHEIFQEF INSMPPRFVS LFKEAVNFSA YSSERWNTFL 250 260 270 280 290 300 YRARAETEVD YNQFLSDKAH KIFMLEGDYM RPTQAEIDKG WELMSQRVYT EREIITDVTK 310 320 330 340 350 360 QKPSIHFIWV KNADRKLIGS TAKLIYLSNS LQSITEQSTW TDALKAIGKS MDIDGKVGQY 370 380 390 400 410 420 ETLCAERKMI ARSTGKKVDN KRLEAVKIGN ALVLWEQQFI LANDLFKNQE RQKFMKNFFG 430 440 450 460 470 480 IGKHKSFKDK TSSDIETDKP KILDFNNTIV LMAARTMVNK NKALLAKDNT LQDLHPIIMQ 490 500 510 520 530 540 YASEIKEASK DTFDALLKIS KTCFWQCIVD VSTIMRNILA VSQYNRHNTF RVAMCANDSV 550 560 570 580 590 600 YALVFPSSDI KTKRATVVFS IVCMHKEKND LMDAGALFTT LECKNKEYIS ISKAIRLDKE 610 620 630 640 650 660 RCQRIVSSPG LFILSSMLLY NNNPEVNLVD VLNFTFYTSL SITKSMLSLT EPSRYMIMNS 670 680 690 700 710 720 LAISSHVRDY IAEKFSPYTK TLFSVYMVNL IKRGCASANE QSSKIQLRNI YLSDYDITQK 730 740 750 760 770 780 GVNDGRNLDS IWFPGKVNLK EYINQIYLPF YFNAKGLHEK HHVMIDLAKT VLEIEMNQRS 790 800 810 820 830 840 DNLGIWSKAE KKQHVNLPIL IHSIAKSLIL DTSRHNHLRN RVESRNNFRR SITTISTFTS 850 860 870 880 890 900 SKSCIKIGDF REIKDKETEK SKKSTEKFDK KFRLSNPLFL EDEEANLEVQ HCNYRALIQK 910 920 930 940 950 960 IPNYKDYISV KVFDRLYELL KNGVLTDKPF IELAMEMMKN HKEFSFTFFN KGQKTAKDRE 970 980 990 1000 1010 1020 IFVGEFEAKM CMYVVERISK ERCKLNTDEM ISEPGDSKLK ILEKKAEEEI RYIVERTKDS 1030 1040 1050 1060 1070 1080 IIKGDPSKAL KLEINADMSK WSAQDVFYKY FWLIAMDPIL YPAEKTRILY FMCNYMQKLL 1090 1100 1110 1120 1130 1140 ILPDDLIANI LDQKRPYNDD LILEMTNGLN YNYVQIKRNW LQGNFNYISS YVHSCAMLVY 1150 1160 1170 1180 1190 1200 KDILKECMKL LDGDCLINSM VHSDDNQTSL AIIQNKVSDQ IVIQYAANTF ESVCLTFGCQ 1210 1220 1230 1240 1250 1260 ANMKKTYITH TCKEFVSLFN LHGEPLSVFG RFLLPSVGDC AYIGPYEDLA SRLSAAQQSL 1270 1280 1290 1300 1310 1320 KHGCPPSLVW LAISCSHWIT FFTYNMLDDQ INAPQQHLPF NNRKEIPVEL NGYLNAPLYL 1330 1340 1350 1360 1370 1380 IALVGLEAGN LWFLINILKK LVPLDKQKET IQSQCLHLCN SIDKLTESEK FKLKILRYLT 1390 1400 1410 1420 1430 1440 LDTEMSVDNN MGETSDMRSR SLLTPRKFTT LGSLNKLVSY NDFRSSLDDQ RFTDNLNFML 1450 1460 1470 1480 1490 1500 NNPELLVTKG ENKEQFMQSV LFRYNSKRFK ESLSIQNPAQ LFIEQILFSH KPIIDYSSIF 1510 1520 1530 1540 1550 1560 DKLTSLAEAD IIEELPEIIG RVTFPQAYQM INRDIGQLPL DIDDIKLIFR YCILNDPLMI 1570 1580 1590 1600 1610 1620 TAANTSLLCV KGTPQDRTGL SASQMPEFRN MKLIHHSPAL VLKAFSKGTS DIPGADPIEL 1630 1640 1650 1660 1670 1680 EKDLHHLNEF VETTAIKEKI LHNIDNPPKH LIGNEILIYR IREMTKLYQV CYDYVKSTEH 1690 1700 1710 1720 1730 1740 KVKIFILPMK SYTAIDFCTL IQGNTISDNK WYTMHYLKQI ASGSIKGNIV TTSTSEQIIA 1750 1760 1770 1780 1790 1800 NECFRVLCHF ADSFVEEASR LSFINEVLDN FTYKNISVNS LFNTLLASTT RLDFIPLLFR 1810 1820 1830 1840 1850 1860 LKVLTQTDLN RFDALKTNER VSWNNWQTNR SLNSGLIDLT ISGYLRSIRV VGEDNKLKIA 1870 1880 1890 1900 1910 1920 ELTIPNFYPN TVFHAGNKLL NSRHGLKFEY MEEIVLDEKY NYYITYQKKR AHIYTYQVST 1930 1940 1950 1960 1970 1980 IEHILRRNNE GLQSRGPRYN KMVPVCPVVL SVRDELFRMS LENVFSLNMT NFSMSRLFVS 1990 2000 2010 2020 2030 2040 PDEVATVKKA HMSKMMFFSG PTIKAGIINL TSLMRTQELL TLNYDNLCKS SIVPFCRILE 2050 2060 2070 2080 2090 2100 CNGDEQGELI FLSDEVMDFT ISEEIESMPL FTIRYQKRGT EIMTYKNAIM KLVSAGVDEI 2110 2120 2130 2140 2150 2160 KEVFDFSKQG FYSKKNLGII NTICSIINIL ETNEWSTILY NSFHIAMLLE SMDREFHMFT 2170 2180 2190 2200 2210 2220 LPEAFFINVA GGVVNWTKLL KFIKSLPVIE QEPWSMMMSR FVEKTVYLIE REMNKDVDFT 2230 DFLDELEFSS GKSLFTFF

References

[1]	"Nucleotide sequence analysis of the large (L) genomic RNA segment of Bunyamwera virus, the prototype of the family Bunyaviridae." Elliott R.M. Virology 173:426-436(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14383 Genomic RNA. Translation: CAA32553.1.
PIR	RRVUBY. A33744.
RefSeq	NP_047211.1. NC_001925.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2648215.
Family and domain databases
InterPro	IPR026382. CapSnatchArena. IPR007099. RNA-dir_pol_NSvirus. IPR014384. RNA-dir_pol_orthobunyavirus. IPR007322. RNA_pol_bunyavir. [Graphical view]
Pfam	PF04196. Bunya_RdRp. 1 hit. [Graphical view]
PIRSF	PIRSF000824. L_OrthobunV. 1 hit.
TIGRFAMs	TIGR04202. capSnatchArena. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_BUNYW

Accession

Primary (citable) accession number: P20470

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	April 3, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program