##gff-version 3
P20457	UniProtKB	Chain	1	528	.	.	.	ID=PRO_0000046776;Note=DNA primase large subunit	
P20457	UniProtKB	Region	210	239	.	.	.	Note=H-T-H-like motif;Ontology_term=ECO:0000255;evidence=ECO:0000255	
P20457	UniProtKB	Binding site	336	336	.	.	.	Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20404922;Dbxref=PMID:20404922	
P20457	UniProtKB	Binding site	417	417	.	.	.	Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20404922;Dbxref=PMID:20404922	
P20457	UniProtKB	Binding site	434	434	.	.	.	Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20404922;Dbxref=PMID:20404922	
P20457	UniProtKB	Binding site	474	474	.	.	.	Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20404922;Dbxref=PMID:20404922	
P20457	UniProtKB	Mutagenesis	152	152	.	.	.	Note=Temperature-sensitive. E->Q;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:2023935;Dbxref=PMID:2023935	
P20457	UniProtKB	Mutagenesis	336	336	.	.	.	Note=Mild disruption of iron-sulfur-binding. Strong disruption of iron-sulfur-binding%3B when associated with S-474. Strong disruption of iron-sulfur-binding%2C leading to destabilization of the protein and preventing its purification%3B when associated with S-417 or S-434. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17704817;Dbxref=PMID:17704817	
P20457	UniProtKB	Mutagenesis	401	401	.	.	.	Note=Lethal. H->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:2023935;Dbxref=PMID:2023935	
P20457	UniProtKB	Mutagenesis	417	417	.	.	.	Note=Mild disruption of iron-sulfur-binding. Strong disruption of iron-sulfur-binding%2C leading to destabilization of the protein and preventing its purification%3B when associated with S-336. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17704817;Dbxref=PMID:17704817	
P20457	UniProtKB	Mutagenesis	434	434	.	.	.	Note=Mild disruption of iron-sulfur-binding. Strong disruption of iron-sulfur-binding%2C leading to destabilization of the protein and preventing its purification%3B when associated with S-336. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17704817;Dbxref=PMID:17704817	
P20457	UniProtKB	Mutagenesis	434	434	.	.	.	Note=Temperature-sensitive. C->Y;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17704817;Dbxref=PMID:17704817	
P20457	UniProtKB	Mutagenesis	474	474	.	.	.	Note=Mild disruption of iron-sulfur-binding. Strong disruption of iron-sulfur-binding%3B when associated with S-336. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17704817;Dbxref=PMID:17704817	
P20457	UniProtKB	Beta strand	318	320	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6DTV	
P20457	UniProtKB	Helix	323	325	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6DTV	
P20457	UniProtKB	Helix	327	330	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6DTV	
P20457	UniProtKB	Helix	335	347	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6DTV	
P20457	UniProtKB	Helix	352	365	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6DTV	
P20457	UniProtKB	Helix	369	381	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6DTV	
P20457	UniProtKB	Helix	382	384	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6DTV	
P20457	UniProtKB	Helix	388	394	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6DTV	
P20457	UniProtKB	Helix	396	402	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6DTV	
P20457	UniProtKB	Turn	403	406	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6DTV	
P20457	UniProtKB	Helix	417	422	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6DTV	
P20457	UniProtKB	Helix	435	438	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6DTV	
P20457	UniProtKB	Helix	441	450	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6DTV	
P20457	UniProtKB	Helix	455	466	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6DTV	
P20457	UniProtKB	Helix	470	481	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6DTV	
P20457	UniProtKB	Helix	500	509	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6DTV