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Protein

DNA primase large subunit

Gene

PRI2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. In a complex with DNA polymerase alpha (DNA polymerase alpha:primase) constitutes a replicative polymerase. Both primase components participate in formation of the active center, but the ATP-binding site is exclusively located on p48.

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 1 [4Fe-4S] cluster.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi336Iron-sulfur (4Fe-4S)1 Publication1
Metal bindingi417Iron-sulfur (4Fe-4S)1 Publication1
Metal bindingi434Iron-sulfur (4Fe-4S)1 Publication1
Metal bindingi474Iron-sulfur (4Fe-4S)1 Publication1

GO - Molecular functioni

GO - Biological processi

  • DNA replication Source: SGD
  • DNA replication, synthesis of RNA primer Source: SGD
  • DNA replication initiation Source: SGD
  • double-strand break repair Source: SGD
  • lagging strand elongation Source: SGD

Keywordsi

Molecular functionDNA-binding, Nucleotidyltransferase, Transferase
Biological processDNA replication, Transcription
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31846-MONOMER
ReactomeiR-SCE-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
R-SCE-174411 Polymerase switching on the C-strand of the telomere
R-SCE-174430 Telomere C-strand synthesis initiation
R-SCE-68952 DNA replication initiation
R-SCE-68962 Activation of the pre-replicative complex
R-SCE-69091 Polymerase switching
R-SCE-69166 Removal of the Flap Intermediate
R-SCE-69183 Processive synthesis on the lagging strand

Names & Taxonomyi

Protein namesi
Recommended name:
DNA primase large subunit (EC:2.7.7.-)
Alternative name(s):
DNA polymerase alpha:primase complex p58 subunit
Short name:
DNA polymerase-primase complex p58 subunit
Short name:
Pol alpha-primase complex p58 subunit
DNA primase 58 kDa subunit
Gene namesi
Name:PRI2
Ordered Locus Names:YKL045W
ORF Names:YKL258
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL045W
SGDiS000001528 PRI2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Primosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi152E → Q: Temperature-sensitive. 1 Publication1
Mutagenesisi336C → S: Mild disruption of iron-sulfur-binding. Strong disruption of iron-sulfur-binding; when associated with S-474. Strong disruption of iron-sulfur-binding, leading to destabilization of the protein and preventing its purification; when associated with S-417 or S-434. 1 Publication1
Mutagenesisi401H → S: Lethal. 1 Publication1
Mutagenesisi417C → S: Mild disruption of iron-sulfur-binding. Strong disruption of iron-sulfur-binding, leading to destabilization of the protein and preventing its purification; when associated with S-336. 1 Publication1
Mutagenesisi434C → S: Mild disruption of iron-sulfur-binding. Strong disruption of iron-sulfur-binding, leading to destabilization of the protein and preventing its purification; when associated with S-336. 1 Publication1
Mutagenesisi434C → Y: Temperature-sensitive. 1 Publication1
Mutagenesisi474C → S: Mild disruption of iron-sulfur-binding. Strong disruption of iron-sulfur-binding; when associated with S-336. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000467761 – 528DNA primase large subunitAdd BLAST528

Proteomic databases

MaxQBiP20457
PaxDbiP20457
PRIDEiP20457

Expressioni

Developmental stagei

Fluctuates in amount during the cell cycle.

Interactioni

Subunit structurei

DNA polymerase alpha:primase is a four subunit enzyme complex, which is assembled throughout the cell cycle, and consists of the two DNA polymerase subunits A and B, and the DNA primase large and small subunits. Interacts with MCM10.2 Publications

Protein-protein interaction databases

BioGridi34088, 233 interactors
DIPiDIP-2535N
IntActiP20457, 15 interactors
MINTiP20457
STRINGi4932.YKL045W

Structurei

Secondary structure

1528
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi323 – 325Combined sources3
Helixi327 – 330Combined sources4
Helixi335 – 347Combined sources13
Helixi352 – 364Combined sources13
Helixi369 – 379Combined sources11
Helixi388 – 394Combined sources7
Helixi396 – 402Combined sources7
Helixi417 – 422Combined sources6
Helixi435 – 438Combined sources4
Helixi441 – 450Combined sources10
Helixi455 – 466Combined sources12
Helixi470 – 481Combined sources12
Helixi500 – 510Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LGBX-ray1.54A/B317-512[»]
ProteinModelPortaliP20457
SMRiP20457
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20457

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni210 – 239H-T-H-like motifSequence analysisAdd BLAST30

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000009790
HOGENOMiHOG000212154
InParanoidiP20457
KOiK02685
OMAiDCHGCPY
OrthoDBiEOG092C27SX

Family and domain databases

CDDicd07322 PriL_PriS_Eukaryotic, 1 hit
InterProiView protein in InterPro
IPR016558 DNA_primase_lsu_euk
IPR007238 DNA_primase_lsu_euk/arc
PANTHERiPTHR10537 PTHR10537, 1 hit
PfamiView protein in Pfam
PF04104 DNA_primase_lrg, 1 hit
PIRSFiPIRSF009449 DNA_primase_large_subunit, 1 hit

Sequencei

Sequence statusi: Complete.

P20457-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRQSKRRIA SRKNFSSYDD IVKSELDVGN TNAANQIILS SSSSEEEKKL
60 70 80 90 100
YARLYESKLS FYDLPPQGEI TLEQFEIWAI DRLKILLEIE SCLSRNKSIK
110 120 130 140 150
EIETIIKPQF QKLLPFNTES LEDRKKDYYS HFILRLCFCR SKELREKFVR
160 170 180 190 200
AETFLFKIRF NMLTSTDQTK FVQSLDLPLL QFISNEEKAE LSHQLYQTVS
210 220 230 240 250
ASLQFQLNLN EEHQRKQYFQ QEKFIKLPFE NVIELVGNRL VFLKDGYAYL
260 270 280 290 300
PQFQQLNLLS NEFASKLNQE LIKTYQYLPR LNEDDRLLPI LNHLSSGYTI
310 320 330 340 350
ADFNQQKANQ FSENVDDEIN AQSVWSEEIS SNYPLCIKNL MEGLKKNHHL
360 370 380 390 400
RYYGRQQLSL FLKGIGLSAD EALKFWSEAF TRNGNMTMEK FNKEYRYSFR
410 420 430 440 450
HNYGLEGNRI NYKPWDCHTI LSKPRPGRGD YHGCPFRDWS HERLSAELRS
460 470 480 490 500
MKLTQAQIIS VLDSCQKGEY TIACTKVFEM THNSASADLE IGEQTHIAHP
510 520
NLYFERSRQL QKKQQKLEKE KLFNNGNH
Length:528
Mass (Da):62,263
Last modified:February 1, 1991 - v1
Checksum:i2DA521AA104D3D16
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27209 Genomic DNA Translation: AAA34900.1
X71621 Genomic DNA Translation: CAA50626.1
Z28045 Genomic DNA Translation: CAA81880.1
BK006944 Genomic DNA Translation: DAA09112.1
PIRiA32497
RefSeqiNP_012879.1, NM_001179611.1

Genome annotation databases

EnsemblFungiiYKL045W; YKL045W; YKL045W
GeneIDi853821
KEGGisce:YKL045W

Similar proteinsi

Entry informationi

Entry nameiPRI2_YEAST
AccessioniPrimary (citable) accession number: P20457
Secondary accession number(s): D6VXP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: May 23, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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