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P20457

- PRI2_YEAST

UniProt

P20457 - PRI2_YEAST

Protein

DNA primase large subunit

Gene

PRI2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. In a complex with DNA polymerase alpha (DNA polymerase alpha:primase) constitutes a replicative polymerase. Both primase components participate in formation of the active center, but the ATP-binding site is exclusively located on p48.

    Cofactori

    Binds 1 4Fe-4S cluster.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi336 – 3361Iron-sulfur (4Fe-4S)1 Publication
    Metal bindingi417 – 4171Iron-sulfur (4Fe-4S)1 Publication
    Metal bindingi434 – 4341Iron-sulfur (4Fe-4S)1 Publication
    Metal bindingi474 – 4741Iron-sulfur (4Fe-4S)1 Publication

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. DNA primase activity Source: InterPro
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA replication Source: SGD
    2. DNA replication, synthesis of RNA primer Source: SGD
    3. DNA replication initiation Source: SGD
    4. double-strand break repair Source: SGD
    5. lagging strand elongation Source: SGD

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication, Transcription

    Keywords - Ligandi

    4Fe-4S, DNA-binding, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31846-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA primase large subunit (EC:2.7.7.-)
    Alternative name(s):
    DNA polymerase alpha:primase complex p58 subunit
    Short name:
    DNA polymerase-primase complex p58 subunit
    Short name:
    Pol alpha-primase complex p58 subunit
    DNA primase 58 kDa subunit
    Gene namesi
    Name:PRI2
    Ordered Locus Names:YKL045W
    ORF Names:YKL258
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL045w.
    SGDiS000001528. PRI2.

    Subcellular locationi

    GO - Cellular componenti

    1. alpha DNA polymerase:primase complex Source: SGD
    2. nuclear envelope Source: SGD
    3. nucleus Source: SGD
    4. primosome complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    DNA-directed RNA polymerase, Primosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi152 – 1521E → Q: Temperature-sensitive. 1 Publication
    Mutagenesisi336 – 3361C → S: Mild disruption of iron-sulfur-binding. Strong disruption of iron-sulfur-binding; when associated with S-474. Strong disruption of iron-sulfur-binding, leading to destabilization of the protein and preventing its purification; when associated with S-417 or S-434. 2 Publications
    Mutagenesisi401 – 4011H → S: Lethal. 1 Publication
    Mutagenesisi417 – 4171C → S: Mild disruption of iron-sulfur-binding. Strong disruption of iron-sulfur-binding, leading to destabilization of the protein and preventing its purification; when associated with S-336. 2 Publications
    Mutagenesisi434 – 4341C → S: Mild disruption of iron-sulfur-binding. Strong disruption of iron-sulfur-binding, leading to destabilization of the protein and preventing its purification; when associated with S-336. 2 Publications
    Mutagenesisi434 – 4341C → Y: Temperature-sensitive. 2 Publications
    Mutagenesisi474 – 4741C → S: Mild disruption of iron-sulfur-binding. Strong disruption of iron-sulfur-binding; when associated with S-336. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 528528DNA primase large subunitPRO_0000046776Add
    BLAST

    Proteomic databases

    MaxQBiP20457.
    PaxDbiP20457.

    Expressioni

    Developmental stagei

    Fluctuates in amount during the cell cycle.

    Gene expression databases

    GenevestigatoriP20457.

    Interactioni

    Subunit structurei

    DNA polymerase alpha:primase is a four subunit enzyme complex, which is assembled throughout the cell cycle, and consists of the two DNA polymerase subunits A and B, and the DNA primase large and small subunits. Interacts with MCM10.2 Publications

    Protein-protein interaction databases

    BioGridi34088. 38 interactions.
    DIPiDIP-2535N.
    IntActiP20457. 14 interactions.
    MINTiMINT-633434.
    STRINGi4932.YKL045W.

    Structurei

    Secondary structure

    1
    528
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi323 – 3253
    Helixi327 – 3304
    Helixi335 – 34713
    Helixi352 – 36413
    Helixi369 – 37911
    Helixi388 – 3947
    Helixi396 – 4027
    Helixi417 – 4226
    Helixi435 – 4384
    Helixi441 – 45010
    Helixi455 – 46612
    Helixi470 – 48112
    Helixi500 – 51011

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3LGBX-ray1.54A/B317-512[»]
    ProteinModelPortaliP20457.
    SMRiP20457. Positions 59-293, 317-512.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP20457.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni210 – 23930H-T-H-like motifSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2219.
    GeneTreeiENSGT00390000009790.
    HOGENOMiHOG000212154.
    KOiK02685.
    OMAiMKIIMSN.
    OrthoDBiEOG70S7FC.

    Family and domain databases

    InterProiIPR016558. DNA_primase_lsu_euk.
    IPR007238. DNA_primase_lsu_euk/arc.
    [Graphical view]
    PfamiPF04104. DNA_primase_lrg. 1 hit.
    [Graphical view]
    PIRSFiPIRSF009449. DNA_primase_large_subunit. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P20457-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFRQSKRRIA SRKNFSSYDD IVKSELDVGN TNAANQIILS SSSSEEEKKL    50
    YARLYESKLS FYDLPPQGEI TLEQFEIWAI DRLKILLEIE SCLSRNKSIK 100
    EIETIIKPQF QKLLPFNTES LEDRKKDYYS HFILRLCFCR SKELREKFVR 150
    AETFLFKIRF NMLTSTDQTK FVQSLDLPLL QFISNEEKAE LSHQLYQTVS 200
    ASLQFQLNLN EEHQRKQYFQ QEKFIKLPFE NVIELVGNRL VFLKDGYAYL 250
    PQFQQLNLLS NEFASKLNQE LIKTYQYLPR LNEDDRLLPI LNHLSSGYTI 300
    ADFNQQKANQ FSENVDDEIN AQSVWSEEIS SNYPLCIKNL MEGLKKNHHL 350
    RYYGRQQLSL FLKGIGLSAD EALKFWSEAF TRNGNMTMEK FNKEYRYSFR 400
    HNYGLEGNRI NYKPWDCHTI LSKPRPGRGD YHGCPFRDWS HERLSAELRS 450
    MKLTQAQIIS VLDSCQKGEY TIACTKVFEM THNSASADLE IGEQTHIAHP 500
    NLYFERSRQL QKKQQKLEKE KLFNNGNH 528
    Length:528
    Mass (Da):62,263
    Last modified:February 1, 1991 - v1
    Checksum:i2DA521AA104D3D16
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27209 Genomic DNA. Translation: AAA34900.1.
    X71621 Genomic DNA. Translation: CAA50626.1.
    Z28045 Genomic DNA. Translation: CAA81880.1.
    BK006944 Genomic DNA. Translation: DAA09112.1.
    PIRiA32497.
    RefSeqiNP_012879.1. NM_001179611.1.

    Genome annotation databases

    EnsemblFungiiYKL045W; YKL045W; YKL045W.
    GeneIDi853821.
    KEGGisce:YKL045W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27209 Genomic DNA. Translation: AAA34900.1 .
    X71621 Genomic DNA. Translation: CAA50626.1 .
    Z28045 Genomic DNA. Translation: CAA81880.1 .
    BK006944 Genomic DNA. Translation: DAA09112.1 .
    PIRi A32497.
    RefSeqi NP_012879.1. NM_001179611.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3LGB X-ray 1.54 A/B 317-512 [» ]
    ProteinModelPortali P20457.
    SMRi P20457. Positions 59-293, 317-512.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34088. 38 interactions.
    DIPi DIP-2535N.
    IntActi P20457. 14 interactions.
    MINTi MINT-633434.
    STRINGi 4932.YKL045W.

    Proteomic databases

    MaxQBi P20457.
    PaxDbi P20457.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL045W ; YKL045W ; YKL045W .
    GeneIDi 853821.
    KEGGi sce:YKL045W.

    Organism-specific databases

    CYGDi YKL045w.
    SGDi S000001528. PRI2.

    Phylogenomic databases

    eggNOGi COG2219.
    GeneTreei ENSGT00390000009790.
    HOGENOMi HOG000212154.
    KOi K02685.
    OMAi MKIIMSN.
    OrthoDBi EOG70S7FC.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31846-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P20457.
    NextBioi 975005.
    PROi P20457.

    Gene expression databases

    Genevestigatori P20457.

    Family and domain databases

    InterProi IPR016558. DNA_primase_lsu_euk.
    IPR007238. DNA_primase_lsu_euk/arc.
    [Graphical view ]
    Pfami PF04104. DNA_primase_lrg. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF009449. DNA_primase_large_subunit. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A single essential gene, PRI2, encodes the large subunit of DNA primase in Saccharomyces cerevisiae."
      Foiani M., Santocanale C., Plevani P., Lucchini G.
      Mol. Cell. Biol. 9:3081-3087(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The sequence of a 17.5 kb DNA fragment on the left arm of yeast chromosome XI identifies the protein kinase gene ELM1, the DNA primase gene PRI2, a new gene encoding a putative histone and seven new open reading frames."
      Purnelle B., Tettelin H., van Dyck L., Skala J., Goffeau A.
      Yeast 9:1379-1384(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: COMPOSITION OF THE DNA POLYMERASE ALPHA:PRIMASE COMPLEX.
    6. "Mutations in conserved yeast DNA primase domains impair DNA replication in vivo."
      Francesconi S., Longhese M.P., Piseri A., Santocanale C., Lucchini G., Plevani P.
      Proc. Natl. Acad. Sci. U.S.A. 88:3877-3881(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.
    7. "A conserved Hsp10-like domain in Mcm10 is required to stabilize the catalytic subunit of DNA polymerase-alpha in budding yeast."
      Ricke R.M., Bielinsky A.-K.
      J. Biol. Chem. 281:18414-18425(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MCM10.
    8. "An iron-sulfur domain of the eukaryotic primase is essential for RNA primer synthesis."
      Klinge S., Hirst J., Maman J.D., Krude T., Pellegrini L.
      Nat. Struct. Mol. Biol. 14:875-877(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IRON-SULFUR-BINDING, COFACTOR, MUTAGENESIS OF CYS-336; CYS-417; CYS-434 AND CYS-474.
    9. "Shared active site architecture between the large subunit of eukaryotic primase and DNA photolyase."
      Sauguet L., Klinge S., Perera R.L., Maman J.D., Pellegrini L.
      PLoS ONE 5:E10083-E10083(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.54 ANGSTROMS) OF 317-512 IN COMPLEX WITH IRON-SULFUR.

    Entry informationi

    Entry nameiPRI2_YEAST
    AccessioniPrimary (citable) accession number: P20457
    Secondary accession number(s): D6VXP2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3