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Protein

G2/mitotic-specific cyclin-B

Gene

CycB

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

GO - Molecular functioni

  • cyclin-dependent protein serine/threonine kinase regulator activity Source: FlyBase

GO - Biological processi

  • attachment of spindle microtubules to kinetochore Source: FlyBase
  • cell separation after cytokinesis Source: FlyBase
  • embryonic development via the syncytial blastoderm Source: FlyBase
  • G2/M transition of mitotic cell cycle Source: FlyBase
  • mitotic chromosome movement towards spindle pole Source: FlyBase
  • mitotic cytokinesis Source: FlyBase
  • regulation of chromatin binding Source: FlyBase
  • syncytial blastoderm mitotic cell cycle Source: FlyBase

Keywordsi

Molecular functionCyclin
Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-DME-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-DME-176408 Regulation of APC/C activators between G1/S and early anaphase
R-DME-176412 Phosphorylation of the APC/C
R-DME-2500257 Resolution of Sister Chromatid Cohesion
R-DME-3301854 Nuclear Pore Complex (NPC) Disassembly
R-DME-4419969 Depolymerisation of the Nuclear Lamina
R-DME-6804114 TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest
R-DME-69273 Cyclin A/B1/B2 associated events during G2/M transition
R-DME-69478 G2/M DNA replication checkpoint
R-DME-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex
R-DME-8878166 Transcriptional regulation by RUNX2
SignaLinkiP20439

Names & Taxonomyi

Protein namesi
Recommended name:
G2/mitotic-specific cyclin-B
Gene namesi
Name:CycB
ORF Names:CG3510
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0000405 CycB

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000803851 – 530G2/mitotic-specific cyclin-BAdd BLAST530

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei137Phosphoserine2 Publications1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP20439
PRIDEiP20439

PTM databases

iPTMnetiP20439

Expressioni

Developmental stagei

Accumulates steadily during G2 and is abruptly destroyed at mitosis.

Gene expression databases

BgeeiFBgn0000405
ExpressionAtlasiP20439 differential
GenevisibleiP20439 DM

Interactioni

Subunit structurei

Interacts with the protein kinase Cdk1 to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex.

Protein-protein interaction databases

BioGridi63230, 88 interactors
IntActiP20439, 54 interactors
MINTiP20439
STRINGi7227.FBpp0071822

Structurei

3D structure databases

ProteinModelPortaliP20439
SMRiP20439
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cyclin family. Cyclin AB subfamily.Curated

Phylogenomic databases

eggNOGiKOG0653 Eukaryota
COG5024 LUCA
GeneTreeiENSGT00760000118939
InParanoidiP20439
KOiK21770
OMAiNDKHWTP
OrthoDBiEOG091G0E9B
PhylomeDBiP20439

Family and domain databases

CDDicd00043 CYCLIN, 2 hits
InterProiView protein in InterPro
IPR013763 Cyclin-like
IPR036915 Cyclin-like_sf
IPR004367 Cyclin_C-dom
IPR006671 Cyclin_N
PfamiView protein in Pfam
PF02984 Cyclin_C, 1 hit
PF00134 Cyclin_N, 1 hit
SMARTiView protein in SMART
SM00385 CYCLIN, 2 hits
SM01332 Cyclin_C, 1 hit
SUPFAMiSSF47954 SSF47954, 2 hits
PROSITEiView protein in PROSITE
PS00292 CYCLINS, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P20439-1) [UniParc]FASTAAdd to basket
Also known as: D

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVGTTLKMRG DENASENFKQ VQLKKLTVPS MEATTKRAAL GDLQNRGISR
60 70 80 90 100
PIAAKDAAQK DSKDLKLTDA LRNAKARVDS HWKKQPLGST NGNGNGAVPP
110 120 130 140 150
KVNEGGVSAF LRSNSVRNRV PTKTTVEPTK VTVKSSSSEN VNEPTLKRED
160 170 180 190 200
SNLSKKSLTK LRAALAKPVM GVSGIRREPV AVSRKEAETK KELPETKKDS
210 220 230 240 250
LEVKKDATRM PLIRGNSAVT TTTSTMPTTM SLSSKRLAGI EDIDANDKEN
260 270 280 290 300
LVLVSEYVND IYDYLYQVEL EQPIHKDHLA GQKEVSHKMR AVLIDWINEV
310 320 330 340 350
HLQFHLAAET FQLAVAIIDR YLQVVKDTKR TYLQLVGVTA LFIATKYEEL
360 370 380 390 400
FPPAIGDFVF ITDDTYTARQ IRQMELQIFK AIDCNLSRPL PIHFLRRYSK
410 420 430 440 450
AAGAEDEHHT MSKYFIELAS VDYEMATYRP SEIAAASLFL SLHLLNGNHR
460 470 480 490 500
AGTGFNDRHW TPTLTFYSRY SAAHLRPITR LIAKLARDAP QAKLKAIYNK
510 520 530
YQGSKFQKIA LRTELTGALM DSIVGQSQRK
Length:530
Mass (Da):59,256
Last modified:June 21, 2005 - v2
Checksum:iD74ABEE0C8066D4A
GO
Isoform B (identifier: P20439-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MVGTTLKMRGDE → MAALEK

Show »
Length:524
Mass (Da):58,580
Checksum:iECFAD5B6DCCF5D0E
GO
Isoform C (identifier: P20439-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.

Show »
Length:500
Mass (Da):55,923
Checksum:i9DAA664A643C9936
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti162R → G in AAA28436 (PubMed:2139805).Curated1
Sequence conflicti383D → N in CAA39148 (PubMed:2142452).Curated1
Sequence conflicti515L → P in CAA39148 (PubMed:2142452).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0215701 – 30Missing in isoform C. CuratedAdd BLAST30
Alternative sequenceiVSP_0215691 – 12MVGTT…MRGDE → MAALEK in isoform B. CuratedAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33192 mRNA Translation: AAA28436.1
X55542 mRNA Translation: CAA39148.1
AJ006773 Genomic DNA Translation: CAA07238.1
AJ006773 Genomic DNA Translation: CAA07239.1
AJ006773 Genomic DNA Translation: CAA07240.1
AE013599 Genomic DNA Translation: AAF46904.1
AE013599 Genomic DNA Translation: AAG22197.1
AE013599 Genomic DNA Translation: AAM71123.1
AE013599 Genomic DNA Translation: AAM71124.1
AY102682 mRNA Translation: AAM27511.1
PIRiA35144
RefSeqiNP_726244.1, NM_166555.3 [P20439-1]
NP_726245.2, NM_166556.3
NP_726246.1, NM_166557.3 [P20439-2]
NP_726247.1, NM_166558.2 [P20439-3]
UniGeneiDm.7295

Genome annotation databases

EnsemblMetazoaiFBtr0071911; FBpp0071822; FBgn0000405 [P20439-1]
FBtr0071913; FBpp0071824; FBgn0000405 [P20439-2]
FBtr0071914; FBpp0071825; FBgn0000405 [P20439-3]
GeneIDi37618
KEGGidme:Dmel_CG3510

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCCNB_DROME
AccessioniPrimary (citable) accession number: P20439
Secondary accession number(s): Q0E8Y2
, Q8MME2, Q9I7V2, Q9TYH6, Q9TYH7, Q9V3F4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: June 21, 2005
Last modified: March 28, 2018
This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health