P20438 (CG12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G1/S-specific cyclin CLN2

Gene names

Name:	CLN2
Ordered Locus Names:	YPL256C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	545 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for the control of the cell cycle at the G1/S (start) transition. Interacts with the CDC28 protein kinase to form MPF.
Developmental stage	CLN1 and CLN2 mRNAs fluctuate periodically in the cell cycle, peaking in G1 phase.
Miscellaneous	A dominant mutation in CLN2 gene (CLN2-1), advances the G1- to S-phase transition in cycling cells and impairs the ability of cells to arrest in G1 phase in response to external signals. Present with 1270 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the cyclin family.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Molecular function	Cyclin
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW re-entry into mitotic cell cycle after pheromone arrest Inferred from genetic interaction PubMed 9927449. Source: SGD regulation of cyclin-dependent protein serine/threonine kinase activity Inferred from direct assay PubMed 10409718PubMed 7862657PubMed 8387915. Source: SGD
Cellular_component	cyclin-dependent protein kinase holoenzyme complex Inferred from direct assay PubMed 2142620. Source: SGD cytoplasm Inferred from direct assay PubMed 10611233PubMed 11080155PubMed 11792824. Source: SGD nucleus Inferred from direct assay PubMed 10611233PubMed 11080155PubMed 11792824. Source: SGD
Molecular_function	cyclin-dependent protein serine/threonine kinase regulator activity Inferred from direct assay PubMed 10409718PubMed 7862657PubMed 8387915. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 545	545	G1/S-specific cyclin CLN2		PRO_0000080412
Experimental info
	Sequence conflict	440	1	Q → R in AAT93248. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P20438 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: D6426B94E040E960	FASTA	545	61,697
	10 20 30 40 50 60 MASAEPRPRM GLVINAKPDY YPIELSNAEL LSHFEMLQEY HQEISTNVIA QSCKFKPNPK 70 80 90 100 110 120 LIDQQPEMNP VETRSNIITF LFELSVVTRV TNGIFFHSVR LYDRYCSKRI VLRDQAKLVV 130 140 150 160 170 180 ATCLWLAAKT WGGCNHIINN VVIPTGGRFY GPNPRARIPR LSELVHYCGD GQVFDESMFL 190 200 210 220 230 240 QMERHILDTL NWNIYEPMIN DYVLNVDENC LMQYELYENQ VTYDKQCSEK RQSQLSQDSD 250 260 270 280 290 300 ATVDERPYQN EEEEEEDLKL KIKLINLKKF LIDVSAWQYD LLRYELFEVS HGIFSIINQF 310 320 330 340 350 360 TNQDHGPFLM TPMTSESKNG EILSTLMNGI VSIPNSLMEV YKTVNGVLPF INQVKEYHLD 370 380 390 400 410 420 LQRKLQIASN LNISRKLTIS TPSCSFENSN STSIPSPASS SQSHTPMRNM SSLSDNSVFS 430 440 450 460 470 480 RNMEQSSPIT PSMYQFGQQQ SNSICGSTVS VNSLVNTNNK QRIYEQITGP NSNNATNDYI 490 500 510 520 530 540 DLLNLNESNK ENQNPATAHY LNGGPPKTSF INHGMFPSPT GTINSGKSSS ASSLISFGMG NTQVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A family of cyclin homologs that control the G1 phase in yeast." Hadwiger J.A., Wittenberg C., Richardson H.E., de Barros Lopes M., Reed S.I. Proc. Natl. Acad. Sci. U.S.A. 86:6255-6259(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence of the Saccharomyces cerevisiae CLN1 and CLN2 genes." Hadwiger J.A., Reed S.I. Nucleic Acids Res. 18:4025-4025(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Wittenberg C., Chapman-Shimshoni D. Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M33265 Genomic DNA. Translation: AAA65725.1. Z73612 Genomic DNA. Translation: CAA97982.1. AY693229 Genomic DNA. Translation: AAT93248.1. BK006949 Genomic DNA. Translation: DAA11181.1.
PIR	COBYC2. B33289.
RefSeq	NP_015067.1. NM_001184070.1.
3D structure databases
ProteinModelPortal	P20438.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2270N.
IntAct	P20438. 11 interactions.
MINT	MINT-583803.
STRING	4932.YPL256C.
Proteomic databases
PaxDb	P20438.
PeptideAtlas	P20438.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL256C; YPL256C; YPL256C.
GeneID	855819.
KEGG	sce:YPL256C.
Organism-specific databases
CYGD	YPL256c.
SGD	S000006177. CLN2.
Phylogenomic databases
eggNOG	COG5024.
GeneTree	ENSGT00390000006123.
HOGENOM	HOG000111606.
KO	K06650.
OMA	PKLIDQQ.
OrthoDB	EOG498Z8K.
Gene expression databases
Genevestigator	P20438.
GermOnline	YPL256C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.472.10. 1 hit.
InterPro	IPR013763. Cyclin-like. IPR014399. Cyclin_CLN/Puc1. IPR006671. Cyclin_N. [Graphical view]
Pfam	PF00134. Cyclin_N. 1 hit. [Graphical view]
PIRSF	PIRSF001770. Cyclin_CLN. 1 hit.
SMART	SM00385. CYCLIN. 1 hit. [Graphical view]
SUPFAM	SSF47954. Cyclin_like. 1 hit.
PROSITE	PS00292. CYCLINS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980359.

Entry information

Entry name

CG12_YEAST

Accession

Primary (citable) accession number: P20438
Secondary accession number(s): D6W3B5, E9P938

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program