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P20436

- RPAB3_YEAST

UniProt

P20436 - RPAB3_YEAST

Protein

DNA-directed RNA polymerases I, II, and III subunit RPABC3

Gene

RPB8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 150 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    DNA-dependent RNA polymerases catalyze the transcription. of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.3 Publications

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. RNA polymerase I activity Source: UniProtKB

    GO - Biological processi

    1. ribosome biogenesis Source: UniProtKB-KW
    2. transcription, RNA-templated Source: GOC
    3. transcription from RNA polymerase III promoter Source: SGD
    4. transcription from RNA polymerase II promoter Source: SGD
    5. transcription from RNA polymerase I promoter Source: UniProtKB
    6. tRNA transcription from RNA polymerase III promoter Source: SGD

    Keywords - Biological processi

    Ribosome biogenesis, Transcription

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33723-MONOMER.
    ReactomeiREACT_191540. mRNA Splicing - Minor Pathway.
    REACT_88842. RNA Polymerase I Transcription Initiation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-directed RNA polymerases I, II, and III subunit RPABC3
    Short name:
    RNA polymerases I, II, and III subunit ABC3
    Alternative name(s):
    ABC14.4
    ABC14.5
    DNA-directed RNA polymerases I, II, and III 14.5 kDa polypeptide
    Gene namesi
    Name:RPB8
    Ordered Locus Names:YOR224C
    ORF Names:YOR50-14
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR224c.
    SGDiS000005750. RPB8.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. DNA-directed RNA polymerase I complex Source: UniProtKB
    2. DNA-directed RNA polymerase II, core complex Source: SGD
    3. DNA-directed RNA polymerase III complex Source: SGD
    4. nucleoplasm Source: Reactome

    Keywords - Cellular componenti

    DNA-directed RNA polymerase, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 146145DNA-directed RNA polymerases I, II, and III subunit RPABC3PRO_0000074001Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei68 – 681Phosphothreonine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP20436.
    PaxDbiP20436.
    PeptideAtlasiP20436.

    Expressioni

    Gene expression databases

    GenevestigatoriP20436.

    Interactioni

    Subunit structurei

    Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes. Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits: RPA135, RPA190, RPC40, RPA14, RPB5, RPO26, RPA43, RPB8, RPA12, RPB10, RPC19, RPC10, RPA49 and RPA34. The complex is composed of a horseshoe-shaped core containing ten subunits (RPA135, RPA190, RPB5, RPO26, RPB8, RPB10, RPC10, RPA12, RPC19 and RPC40) where RPA135 and RPA190 form the DNA-binding cleft. Outside of the core, RPA14 and RPA43 form the stalk that mediates interactions with transcription initiation factors and newly synthesized RNA. Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits: RPO21, RPB2, RPB3, RPB4, RPB5, RPO26, RPB7, RPB8, RPB9, RPB10 and RPC10. Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits. Directly interacts with POLR2A.7 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RSP5P399403EBI-15794,EBI-16219

    Protein-protein interaction databases

    BioGridi34618. 72 interactions.
    DIPiDIP-2195N.
    IntActiP20436. 20 interactions.
    MINTiMINT-507506.
    STRINGi4932.YOR224C.

    Structurei

    Secondary structure

    1
    146
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 1610
    Beta strandi21 – 3212
    Beta strandi37 – 437
    Helixi44 – 463
    Beta strandi54 – 585
    Turni84 – 863
    Helixi89 – 924
    Beta strandi93 – 1019
    Beta strandi108 – 1103
    Beta strandi112 – 1187
    Beta strandi121 – 1288
    Turni129 – 1335
    Beta strandi136 – 1383
    Beta strandi140 – 1456

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1A1DNMR-A1-146[»]
    1I3QX-ray3.10H1-146[»]
    1I50X-ray2.80H1-146[»]
    1I6HX-ray3.30H1-146[»]
    1K83X-ray2.80H1-146[»]
    1NIKX-ray4.10H1-146[»]
    1NT9X-ray4.20H1-146[»]
    1PQVX-ray3.80H1-146[»]
    1R5UX-ray4.50H1-146[»]
    1R9SX-ray4.25H1-146[»]
    1R9TX-ray3.50H1-146[»]
    1SFOX-ray3.61H1-146[»]
    1TWAX-ray3.20H1-146[»]
    1TWCX-ray3.00H1-146[»]
    1TWFX-ray2.30H1-146[»]
    1TWGX-ray3.30H1-146[»]
    1TWHX-ray3.40H1-146[»]
    1WCMX-ray3.80H1-146[»]
    1Y1VX-ray3.80H1-146[»]
    1Y1WX-ray4.00H1-146[»]
    1Y1YX-ray4.00H1-146[»]
    1Y77X-ray4.50H1-146[»]
    2B63X-ray3.80H1-146[»]
    2B8KX-ray4.15H1-146[»]
    2E2HX-ray3.95H1-146[»]
    2E2IX-ray3.41H1-146[»]
    2E2JX-ray3.50H1-146[»]
    2JA5X-ray3.80H1-146[»]
    2JA6X-ray4.00H1-146[»]
    2JA7X-ray3.80H/T1-146[»]
    2JA8X-ray3.80H1-146[»]
    2NVQX-ray2.90H1-146[»]
    2NVTX-ray3.36H1-146[»]
    2NVXX-ray3.60H1-146[»]
    2NVYX-ray3.40H1-146[»]
    2NVZX-ray4.30H1-146[»]
    2R7ZX-ray3.80H1-146[»]
    2R92X-ray3.80H1-146[»]
    2R93X-ray4.00H1-146[»]
    2VUMX-ray3.40H1-146[»]
    2YU9X-ray3.40H1-146[»]
    3CQZX-ray2.80H1-146[»]
    3FKIX-ray3.88H1-146[»]
    3GTGX-ray3.78H1-146[»]
    3GTJX-ray3.42H1-146[»]
    3GTKX-ray3.80H1-146[»]
    3GTLX-ray3.38H1-146[»]
    3GTMX-ray3.80H1-146[»]
    3GTOX-ray4.00H1-146[»]
    3GTPX-ray3.90H1-146[»]
    3GTQX-ray3.80H1-146[»]
    3H3VX-ray4.00I1-146[»]
    3HOUX-ray3.20H/T1-146[»]
    3HOVX-ray3.50H1-146[»]
    3HOWX-ray3.60H1-146[»]
    3HOXX-ray3.65H1-146[»]
    3HOYX-ray3.40H1-146[»]
    3HOZX-ray3.65H1-146[»]
    3I4MX-ray3.70H1-146[»]
    3I4NX-ray3.90H1-146[»]
    3J0Kelectron microscopy36.00H1-146[»]
    3J1Nelectron microscopy16.00H1-146[»]
    3K1FX-ray4.30H1-146[»]
    3K7AX-ray3.80H1-146[»]
    3M3YX-ray3.18H1-146[»]
    3M4OX-ray3.57H1-146[»]
    3PO2X-ray3.30H1-146[»]
    3PO3X-ray3.30H1-146[»]
    3QT1X-ray4.30H1-146[»]
    3RZDX-ray3.30H1-146[»]
    3RZOX-ray3.00H1-146[»]
    3S14X-ray2.85H1-146[»]
    3S15X-ray3.30H1-146[»]
    3S16X-ray3.24H1-146[»]
    3S17X-ray3.20H1-146[»]
    3S1MX-ray3.13H1-146[»]
    3S1NX-ray3.10H1-146[»]
    3S1QX-ray3.30H1-146[»]
    3S1RX-ray3.20H1-146[»]
    3S2DX-ray3.20H1-146[»]
    3S2HX-ray3.30H1-146[»]
    4A3BX-ray3.50H1-146[»]
    4A3CX-ray3.50H1-146[»]
    4A3DX-ray3.40H1-146[»]
    4A3EX-ray3.40H1-146[»]
    4A3FX-ray3.50H1-146[»]
    4A3GX-ray3.50H1-146[»]
    4A3IX-ray3.80H1-146[»]
    4A3JX-ray3.70H1-146[»]
    4A3KX-ray3.50H1-146[»]
    4A3LX-ray3.50H1-146[»]
    4A3MX-ray3.90H1-146[»]
    4A93X-ray3.40H1-146[»]
    4BBRX-ray3.40H1-146[»]
    4BBSX-ray3.60H1-146[»]
    4BXXX-ray3.28H1-146[»]
    4BXZX-ray4.80H1-146[»]
    4BY1X-ray3.60H1-146[»]
    4BY7X-ray3.15H1-146[»]
    4C2MX-ray2.80H/W1-146[»]
    4C3HX-ray3.27H1-146[»]
    4C3IX-ray3.0H1-146[»]
    4C3JX-ray3.35H1-146[»]
    ProteinModelPortaliP20436.
    SMRiP20436. Positions 1-146.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP20436.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni16 – 3924Non-specific ssDNA bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG279425.
    GeneTreeiENSGT00390000018195.
    HOGENOMiHOG000175572.
    KOiK03016.
    OMAiWRPDGKG.
    OrthoDBiEOG78D7XD.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    InterProiIPR012340. NA-bd_OB-fold.
    IPR005570. RNA_pol_Rpb8.
    [Graphical view]
    PANTHERiPTHR10917. PTHR10917. 1 hit.
    PfamiPF03870. RNA_pol_Rpb8. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000779. RNA_pol_Rpb8. 1 hit.
    SMARTiSM00658. RPOL8c. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P20436-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNTLFDDIF QVSEVDPGRY NKVCRIEAAS TTQDQCKLTL DINVELFPVA    50
    AQDSLTVTIA SSLNLEDTPA NDSSATRSWR PPQAGDRSLA DDYDYVMYGT 100
    AYKFEEVSKD LIAVYYSFGG LLMRLEGNYR NLNNLKQENA YLLIRR 146
    Length:146
    Mass (Da):16,511
    Last modified:February 1, 1991 - v1
    Checksum:i5B71693FE6FD8863
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53289 Genomic DNA. Translation: CAA37383.1.
    X92441 Genomic DNA. Translation: CAA63187.1.
    Z75132 Genomic DNA. Translation: CAA99443.1.
    AY693087 Genomic DNA. Translation: AAT93106.1.
    BK006948 Genomic DNA. Translation: DAA10995.1.
    PIRiC34588.
    RefSeqiNP_014867.1. NM_001183643.1.

    Genome annotation databases

    EnsemblFungiiYOR224C; YOR224C; YOR224C.
    GeneIDi854399.
    KEGGisce:YOR224C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53289 Genomic DNA. Translation: CAA37383.1 .
    X92441 Genomic DNA. Translation: CAA63187.1 .
    Z75132 Genomic DNA. Translation: CAA99443.1 .
    AY693087 Genomic DNA. Translation: AAT93106.1 .
    BK006948 Genomic DNA. Translation: DAA10995.1 .
    PIRi C34588.
    RefSeqi NP_014867.1. NM_001183643.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1A1D NMR - A 1-146 [» ]
    1I3Q X-ray 3.10 H 1-146 [» ]
    1I50 X-ray 2.80 H 1-146 [» ]
    1I6H X-ray 3.30 H 1-146 [» ]
    1K83 X-ray 2.80 H 1-146 [» ]
    1NIK X-ray 4.10 H 1-146 [» ]
    1NT9 X-ray 4.20 H 1-146 [» ]
    1PQV X-ray 3.80 H 1-146 [» ]
    1R5U X-ray 4.50 H 1-146 [» ]
    1R9S X-ray 4.25 H 1-146 [» ]
    1R9T X-ray 3.50 H 1-146 [» ]
    1SFO X-ray 3.61 H 1-146 [» ]
    1TWA X-ray 3.20 H 1-146 [» ]
    1TWC X-ray 3.00 H 1-146 [» ]
    1TWF X-ray 2.30 H 1-146 [» ]
    1TWG X-ray 3.30 H 1-146 [» ]
    1TWH X-ray 3.40 H 1-146 [» ]
    1WCM X-ray 3.80 H 1-146 [» ]
    1Y1V X-ray 3.80 H 1-146 [» ]
    1Y1W X-ray 4.00 H 1-146 [» ]
    1Y1Y X-ray 4.00 H 1-146 [» ]
    1Y77 X-ray 4.50 H 1-146 [» ]
    2B63 X-ray 3.80 H 1-146 [» ]
    2B8K X-ray 4.15 H 1-146 [» ]
    2E2H X-ray 3.95 H 1-146 [» ]
    2E2I X-ray 3.41 H 1-146 [» ]
    2E2J X-ray 3.50 H 1-146 [» ]
    2JA5 X-ray 3.80 H 1-146 [» ]
    2JA6 X-ray 4.00 H 1-146 [» ]
    2JA7 X-ray 3.80 H/T 1-146 [» ]
    2JA8 X-ray 3.80 H 1-146 [» ]
    2NVQ X-ray 2.90 H 1-146 [» ]
    2NVT X-ray 3.36 H 1-146 [» ]
    2NVX X-ray 3.60 H 1-146 [» ]
    2NVY X-ray 3.40 H 1-146 [» ]
    2NVZ X-ray 4.30 H 1-146 [» ]
    2R7Z X-ray 3.80 H 1-146 [» ]
    2R92 X-ray 3.80 H 1-146 [» ]
    2R93 X-ray 4.00 H 1-146 [» ]
    2VUM X-ray 3.40 H 1-146 [» ]
    2YU9 X-ray 3.40 H 1-146 [» ]
    3CQZ X-ray 2.80 H 1-146 [» ]
    3FKI X-ray 3.88 H 1-146 [» ]
    3GTG X-ray 3.78 H 1-146 [» ]
    3GTJ X-ray 3.42 H 1-146 [» ]
    3GTK X-ray 3.80 H 1-146 [» ]
    3GTL X-ray 3.38 H 1-146 [» ]
    3GTM X-ray 3.80 H 1-146 [» ]
    3GTO X-ray 4.00 H 1-146 [» ]
    3GTP X-ray 3.90 H 1-146 [» ]
    3GTQ X-ray 3.80 H 1-146 [» ]
    3H3V X-ray 4.00 I 1-146 [» ]
    3HOU X-ray 3.20 H/T 1-146 [» ]
    3HOV X-ray 3.50 H 1-146 [» ]
    3HOW X-ray 3.60 H 1-146 [» ]
    3HOX X-ray 3.65 H 1-146 [» ]
    3HOY X-ray 3.40 H 1-146 [» ]
    3HOZ X-ray 3.65 H 1-146 [» ]
    3I4M X-ray 3.70 H 1-146 [» ]
    3I4N X-ray 3.90 H 1-146 [» ]
    3J0K electron microscopy 36.00 H 1-146 [» ]
    3J1N electron microscopy 16.00 H 1-146 [» ]
    3K1F X-ray 4.30 H 1-146 [» ]
    3K7A X-ray 3.80 H 1-146 [» ]
    3M3Y X-ray 3.18 H 1-146 [» ]
    3M4O X-ray 3.57 H 1-146 [» ]
    3PO2 X-ray 3.30 H 1-146 [» ]
    3PO3 X-ray 3.30 H 1-146 [» ]
    3QT1 X-ray 4.30 H 1-146 [» ]
    3RZD X-ray 3.30 H 1-146 [» ]
    3RZO X-ray 3.00 H 1-146 [» ]
    3S14 X-ray 2.85 H 1-146 [» ]
    3S15 X-ray 3.30 H 1-146 [» ]
    3S16 X-ray 3.24 H 1-146 [» ]
    3S17 X-ray 3.20 H 1-146 [» ]
    3S1M X-ray 3.13 H 1-146 [» ]
    3S1N X-ray 3.10 H 1-146 [» ]
    3S1Q X-ray 3.30 H 1-146 [» ]
    3S1R X-ray 3.20 H 1-146 [» ]
    3S2D X-ray 3.20 H 1-146 [» ]
    3S2H X-ray 3.30 H 1-146 [» ]
    4A3B X-ray 3.50 H 1-146 [» ]
    4A3C X-ray 3.50 H 1-146 [» ]
    4A3D X-ray 3.40 H 1-146 [» ]
    4A3E X-ray 3.40 H 1-146 [» ]
    4A3F X-ray 3.50 H 1-146 [» ]
    4A3G X-ray 3.50 H 1-146 [» ]
    4A3I X-ray 3.80 H 1-146 [» ]
    4A3J X-ray 3.70 H 1-146 [» ]
    4A3K X-ray 3.50 H 1-146 [» ]
    4A3L X-ray 3.50 H 1-146 [» ]
    4A3M X-ray 3.90 H 1-146 [» ]
    4A93 X-ray 3.40 H 1-146 [» ]
    4BBR X-ray 3.40 H 1-146 [» ]
    4BBS X-ray 3.60 H 1-146 [» ]
    4BXX X-ray 3.28 H 1-146 [» ]
    4BXZ X-ray 4.80 H 1-146 [» ]
    4BY1 X-ray 3.60 H 1-146 [» ]
    4BY7 X-ray 3.15 H 1-146 [» ]
    4C2M X-ray 2.80 H/W 1-146 [» ]
    4C3H X-ray 3.27 H 1-146 [» ]
    4C3I X-ray 3.0 H 1-146 [» ]
    4C3J X-ray 3.35 H 1-146 [» ]
    ProteinModelPortali P20436.
    SMRi P20436. Positions 1-146.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34618. 72 interactions.
    DIPi DIP-2195N.
    IntActi P20436. 20 interactions.
    MINTi MINT-507506.
    STRINGi 4932.YOR224C.

    Proteomic databases

    MaxQBi P20436.
    PaxDbi P20436.
    PeptideAtlasi P20436.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR224C ; YOR224C ; YOR224C .
    GeneIDi 854399.
    KEGGi sce:YOR224C.

    Organism-specific databases

    CYGDi YOR224c.
    SGDi S000005750. RPB8.

    Phylogenomic databases

    eggNOGi NOG279425.
    GeneTreei ENSGT00390000018195.
    HOGENOMi HOG000175572.
    KOi K03016.
    OMAi WRPDGKG.
    OrthoDBi EOG78D7XD.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33723-MONOMER.
    Reactomei REACT_191540. mRNA Splicing - Minor Pathway.
    REACT_88842. RNA Polymerase I Transcription Initiation.

    Miscellaneous databases

    EvolutionaryTracei P20436.
    NextBioi 976571.
    PROi P20436.

    Gene expression databases

    Genevestigatori P20436.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    InterProi IPR012340. NA-bd_OB-fold.
    IPR005570. RNA_pol_Rpb8.
    [Graphical view ]
    PANTHERi PTHR10917. PTHR10917. 1 hit.
    Pfami PF03870. RNA_pol_Rpb8. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000779. RNA_pol_Rpb8. 1 hit.
    SMARTi SM00658. RPOL8c. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Subunits shared by eukaryotic nuclear RNA polymerases."
      Woychik N.A., Liao S.-M., Kolodziej P.A., Young R.A.
      Genes Dev. 4:313-323(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence and analysis of a 33 kb fragment from the right arm of chromosome XV of the yeast Saccharomyces cerevisiae."
      Galisson F., Dujon B.
      Yeast 12:877-885(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "The yeast RNA polymerase III transcription machinery: a paradigm for eukaryotic gene activation."
      Chedin S., Ferri M.L., Peyroche G., Andrau J.-C., Jourdain S., Lefebvre O., Werner M., Carles C., Sentenac A.
      Cold Spring Harb. Symp. Quant. Biol. 63:381-389(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW ON THE RNA POL III COMPLEX.
    7. "The A14-A43 heterodimer subunit in yeast RNA pol I and their relationship to Rpb4-Rpb7 pol II subunits."
      Peyroche G., Levillain E., Siaut M., Callebaut I., Schultz P., Sentenac A., Riva M., Carles C.
      Proc. Natl. Acad. Sci. U.S.A. 99:14670-14675(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-68, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    12. "Eukaryotic RNA polymerase subunit RPB8 is a new relative of the OB family."
      Krapp S., Kelly G., Reischi J., Weinzierl R.O.J., Matthews S.
      Nat. Struct. Biol. 5:110-114(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    13. "RNA polymerase II/TFIIF structure and conserved organization of the initiation complex."
      Chung W.H., Craighead J.L., Chang W.H., Ezeokonkwo C., Bareket-Samish A., Kornberg R.D., Asturias F.J.
      Mol. Cell 12:1003-1013(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: ELECTRON MICROSCOPY OF THE RNA POL II/TFIIF COMPLEX.
    14. "Structural basis of transcription: RNA polymerase II at 2.8 A resolution."
      Cramer P., Bushnell D.A., Kornberg R.D.
      Science 292:1863-1876(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE RNA POL II CORE COMPLEX.
    15. "Structural basis of transcription: an RNA polymerase II elongation complex at 3.3 A resolution."
      Gnatt A.L., Cramer P., Fu J., Bushnell D.A., Kornberg R.D.
      Science 292:1876-1882(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS) OF THE RNA POL II CORE COMPLEX.
    16. "Structural basis of transcription: alpha-amanitin-RNA polymerase II cocrystal at 2.8 A resolution."
      Bushnell D.A., Cramer P., Kornberg R.D.
      Proc. Natl. Acad. Sci. U.S.A. 99:1218-1222(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE RNA POL II CORE COMPLEX IN COMPLEX WITH ALPHA-AMANITIN.
    17. "Architecture of the RNA polymerase II-TFIIS complex and implications for mRNA cleavage."
      Kettenberger H., Armache K.J., Cramer P.
      Cell 114:347-357(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.8 ANGSTROMS) OF THE RNA POL II COMPLEX IN COMPLEX WITH DST1.
    18. "Architecture of initiation-competent 12-subunit RNA polymerase II."
      Armache K.J., Kettenberger H., Cramer P.
      Proc. Natl. Acad. Sci. U.S.A. 100:6964-6968(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.2 ANGSTROMS) OF THE RNA POL II COMPLEX.
    19. "Complete, 12-subunit RNA polymerase II at 4.1-A resolution: implications for the initiation of transcription."
      Bushnell D.A., Kornberg R.D.
      Proc. Natl. Acad. Sci. U.S.A. 100:6969-6973(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.1 ANGSTROMS) OF THE RNA POL II CORE COMPLEX.
    20. "Structural basis of transcription: nucleotide selection by rotation in the RNA polymerase II active center."
      Westover K.D., Bushnell D.A., Kornberg R.D.
      Cell 119:481-489(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF THE RNA POL II CORE COMPLEX.
    21. "Complete RNA polymerase II elongation complex structure and its interactions with NTP and TFIIS."
      Kettenberger H., Armache K.J., Cramer P.
      Mol. Cell 16:955-965(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.5 ANGSTROMS).
    22. "Structural basis of transcription: an RNA polymerase II-TFIIB cocrystal at 4.5 Angstroms."
      Bushnell D.A., Westover K.D., Davis R.E., Kornberg R.D.
      Science 303:983-988(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.5 ANGSTROMS) OF THE RNA POL II CORE COMPLEX.
    23. "Structures of complete RNA polymerase II and its subcomplex, Rpb4/7."
      Armache K.J., Mitterweger S., Meinhart A., Cramer P.
      J. Biol. Chem. 280:7131-7134(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.8 ANGSTROMS) OF THE RNA POL II COMPLEX.
    24. "Structural biology of RNA polymerase III: subcomplex C17/25 X-ray structure and 11 subunit enzyme model."
      Jasiak A.J., Armache K.J., Martens B., Jansen R.P., Cramer P.
      Mol. Cell 23:71-81(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF THE POL III CORE COMPLEX.
    25. "Structure of an RNA polymerase II-RNA inhibitor complex elucidates transcription regulation by noncoding RNAs."
      Kettenberger H., Eisenfuhr A., Brueckner F., Theis M., Famulok M., Cramer P.
      Nat. Struct. Mol. Biol. 13:44-48(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.8 ANGSTROMS) OF THE RNA POL II COMPLEX IN COMPLEX WITH INHIBITING NON-CODING RNA.
    26. "Phasing RNA polymerase II using intrinsically bound Zn atoms: an updated structural model."
      Meyer P.A., Ye P., Zhang M., Suh M.H., Fu J.
      Structure 14:973-982(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.15 ANGSTROMS) OF THE RNA POL II COMPLEX.
    27. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (12.00 ANGSTROMS) OF THE POL I COMPLEX, FUNCTION, SUBUNIT.
    28. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE POL I COMPLEX, FUNCTION, SUBUNIT.
    29. "RNA polymerase I structure and transcription regulation."
      Engel C., Sainsbury S., Cheung A.C., Kostrewa D., Cramer P.
      Nature 502:650-655(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE POL I COMPLEX, FUNCTION, SUBUNIT.

    Entry informationi

    Entry nameiRPAB3_YEAST
    AccessioniPrimary (citable) accession number: P20436
    Secondary accession number(s): D6W2S9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 150 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 6210 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3