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Protein

DNA-directed RNA polymerase II subunit RPB4

Gene

RPB4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. The RPB4-RPB7 subcomplex is necessary for promoter-directed transcription initiation but is not required for recruitment of Pol II to active preinitiation complexes and seems to be dispensable for transcription elongation and termination. The RPB4-RPB7 subcomplex recruits FCP1 to Pol II. Involved in DNA repair of damage in the transcribed strand. RPB4 is dispensable under optimal growth conditions, but becomes essential during heat or cold shock and under nutrient depletion. Suppresses the RBP9-mediated transcription-coupled repair (TCR) subpathway of nucleotide excision repair (NER) but facilitates the RAD26-mediated TCR subpathway. Under stress conditions only, involved in mRNA export to the cytoplasm. Involved in mRNA decay. Promotes or enhances the deadenylation process of specific mRNAs and may recruit PAT1 and the LSM1-7 complex to these mRNAs, thus stimulating their decapping and further decay.7 Publications

GO - Molecular functioni

  1. DNA-directed RNA polymerase activity Source: UniProtKB-KW
  2. nucleotide binding Source: InterPro
  3. translation initiation factor binding Source: SGD

GO - Biological processi

  1. DNA repair Source: UniProtKB-KW
  2. mRNA export from nucleus in response to heat stress Source: SGD
  3. nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: SGD
  4. positive regulation of translational initiation Source: SGD
  5. recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex Source: SGD
  6. transcription, RNA-templated Source: GOC
  7. transcription from RNA polymerase II promoter Source: SGD
  8. transcription initiation from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, mRNA processing, Transcription

Enzyme and pathway databases

BioCyciYEAST:G3O-31585-MONOMER.
ReactomeiREACT_273109. Formation of the Early Elongation Complex.
REACT_273984. Dual incision reaction in TC-NER.
REACT_277632. mRNA Capping.
REACT_294700. mRNA Splicing - Minor Pathway.
REACT_296055. RNA Polymerase II Promoter Escape.
REACT_300180. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_310018. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_315180. RNA Polymerase II Pre-transcription Events.
REACT_317581. Processing of Capped Intron-Containing Pre-mRNA.
REACT_322294. RNA Pol II CTD phosphorylation and interaction with CE.
REACT_331999. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_341636. Transcription-coupled NER (TC-NER).
REACT_345860. RNA Polymerase II Transcription Initiation.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase II subunit RPB4
Short name:
RNA polymerase II subunit B4
Alternative name(s):
B32
DNA-directed RNA polymerase II 32 kDa polypeptide
Gene namesi
Name:RPB4
Ordered Locus Names:YJL140W
ORF Names:J0654
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome X

Organism-specific databases

CYGDiYJL140w.
SGDiS000003676. RPB4.

Subcellular locationi

Nucleus. Cytoplasm. CytoplasmP-body
Note: Seems to shuttle between nucleus and cytoplasm in a complex with RPB7.

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. cytoplasmic mRNA processing body Source: SGD
  3. DNA-directed RNA polymerase II, core complex Source: SGD
  4. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 221221DNA-directed RNA polymerase II subunit RPB4PRO_0000073984Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei91 – 911Phosphothreonine1 Publication
Modified residuei92 – 921Phosphothreonine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP20433.
PaxDbiP20433.
PeptideAtlasiP20433.

Expressioni

Gene expression databases

GenevestigatoriP20433.

Interactioni

Subunit structurei

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB4 and RPB7 form a dissociable subcomplex associated with the 10-subunit Pol II core complex. In exponentially proliferating cells, only approximative 20 % of the Pol II complexes contain the RPB4-RPB7 subcomplex. In starving cells, that enter stationary phase, RPB4-RPB7 is associated with Pol II in a stoechiometric manner. The RPB4-RPB7 subcomplex probably associates with TFG1. Interacts with LSM2 and PAT1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NIP1P324975EBI-15777,EBI-8965
RPB7P340874EBI-15777,EBI-15790

Protein-protein interaction databases

BioGridi33617. 121 interactions.
DIPiDIP-55N.
IntActiP20433. 20 interactions.
MINTiMINT-538215.
STRINGi4932.YJL140W.

Structurei

Secondary structure

1
221
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 83Combined sources
Helixi17 – 193Combined sources
Beta strandi24 – 274Combined sources
Helixi31 – 333Combined sources
Beta strandi35 – 384Combined sources
Turni40 – 423Combined sources
Beta strandi44 – 463Combined sources
Beta strandi47 – 504Combined sources
Helixi52 – 7120Combined sources
Turni73 – 753Combined sources
Helixi119 – 13315Combined sources
Helixi139 – 15113Combined sources
Helixi157 – 16812Combined sources
Turni169 – 1713Combined sources
Helixi174 – 1829Combined sources
Helixi188 – 1947Combined sources
Helixi196 – 1983Combined sources
Turni199 – 2013Combined sources
Helixi204 – 21714Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NT9X-ray4.20D1-221[»]
1PQVX-ray3.80D1-85[»]
D115-221[»]
1WCMX-ray3.80D4-221[»]
1Y14X-ray2.30A/C35-221[»]
1Y1VX-ray3.80D1-221[»]
1Y1WX-ray4.00D1-221[»]
1Y1YX-ray4.00D1-221[»]
1Y77X-ray4.50D1-221[»]
2B63X-ray3.80D1-221[»]
2B8KX-ray4.15D1-221[»]
2JA5X-ray3.80D1-221[»]
2JA6X-ray4.00D1-221[»]
2JA7X-ray3.80D/P1-221[»]
2JA8X-ray3.80D1-221[»]
2R7ZX-ray3.80D1-221[»]
2R92X-ray3.80D1-221[»]
2R93X-ray4.00D1-221[»]
2VUMX-ray3.40D1-221[»]
3FKIX-ray3.88D1-221[»]
3H3VX-ray4.00E1-221[»]
3HOUX-ray3.20D/P1-221[»]
3HOVX-ray3.50D1-221[»]
3HOWX-ray3.60D1-221[»]
3HOXX-ray3.65D1-221[»]
3HOYX-ray3.40D1-221[»]
3HOZX-ray3.65D1-221[»]
3I4MX-ray3.70D1-221[»]
3I4NX-ray3.90D1-221[»]
3J0Kelectron microscopy36.00D1-221[»]
3J1Nelectron microscopy16.00D4-221[»]
3K1FX-ray4.30D1-221[»]
3PO2X-ray3.30D1-221[»]
3PO3X-ray3.30D1-221[»]
3QT1X-ray4.30D3-221[»]
4A3BX-ray3.50D1-221[»]
4A3CX-ray3.50D1-221[»]
4A3DX-ray3.40D1-221[»]
4A3EX-ray3.40D1-221[»]
4A3FX-ray3.50D1-221[»]
4A3GX-ray3.50D1-221[»]
4A3IX-ray3.80D1-221[»]
4A3JX-ray3.70D1-221[»]
4A3KX-ray3.50D1-221[»]
4A3LX-ray3.50D1-221[»]
4A3MX-ray3.90D1-221[»]
4A93X-ray3.40D1-221[»]
4BBRX-ray3.40D1-221[»]
4BBSX-ray3.60D1-221[»]
4BXXX-ray3.28D1-221[»]
4BXZX-ray4.80D1-221[»]
4BY1X-ray3.60D1-221[»]
4BY7X-ray3.15D1-221[»]
ProteinModelPortaliP20433.
SMRiP20433. Positions 1-221.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20433.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5250.
GeneTreeiENSGT00390000004912.
HOGENOMiHOG000195281.
InParanoidiP20433.
KOiK03012.
OMAiGTLECED.
OrthoDBiEOG7NW6NW.

Family and domain databases

InterProiIPR010997. HRDC-like.
IPR005574. RNA_pol_II_Rpb4.
IPR006590. RNA_pol_II_Rpb4_core.
[Graphical view]
PfamiPF03874. RNA_pol_Rpb4. 1 hit.
[Graphical view]
SMARTiSM00657. RPOL4c. 1 hit.
[Graphical view]
SUPFAMiSSF47819. SSF47819. 1 hit.

Sequencei

Sequence statusi: Complete.

P20433-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVSTSTFQT RRRRLKKVEE EENAATLQLG QEFQLKQINH QGEEEELIAL
60 70 80 90 100
NLSEARLVIK EALVERRRAF KRSQKKHKKK HLKHENANDE TTAVEDEDDD
110 120 130 140 150
LDEDDVNADD DDFMHSETRE KELESIDVLL EQTTGGNNKD LKNTMQYLTN
160 170 180 190 200
FSRFRDQETV GAVIQLLKST GLHPFEVAQL GSLACDTADE AKTLIPSLNN
210 220
KISDDELERI LKELSNLETL Y
Length:221
Mass (Da):25,414
Last modified:January 31, 1991 - v1
Checksum:i72A8A26871B87775
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27253 Genomic DNA. Translation: AAA34996.1.
X58099 Genomic DNA. Translation: CAA41112.1.
X87371 Genomic DNA. Translation: CAA60815.1.
Z49415 Genomic DNA. Translation: CAA89435.1.
AY557856 Genomic DNA. Translation: AAS56182.1.
BK006943 Genomic DNA. Translation: DAA08660.1.
PIRiA32490.
RefSeqiNP_012395.1. NM_001181573.1.

Genome annotation databases

EnsemblFungiiYJL140W; YJL140W; YJL140W.
GeneIDi853301.
KEGGisce:YJL140W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27253 Genomic DNA. Translation: AAA34996.1.
X58099 Genomic DNA. Translation: CAA41112.1.
X87371 Genomic DNA. Translation: CAA60815.1.
Z49415 Genomic DNA. Translation: CAA89435.1.
AY557856 Genomic DNA. Translation: AAS56182.1.
BK006943 Genomic DNA. Translation: DAA08660.1.
PIRiA32490.
RefSeqiNP_012395.1. NM_001181573.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NT9X-ray4.20D1-221[»]
1PQVX-ray3.80D1-85[»]
D115-221[»]
1WCMX-ray3.80D4-221[»]
1Y14X-ray2.30A/C35-221[»]
1Y1VX-ray3.80D1-221[»]
1Y1WX-ray4.00D1-221[»]
1Y1YX-ray4.00D1-221[»]
1Y77X-ray4.50D1-221[»]
2B63X-ray3.80D1-221[»]
2B8KX-ray4.15D1-221[»]
2JA5X-ray3.80D1-221[»]
2JA6X-ray4.00D1-221[»]
2JA7X-ray3.80D/P1-221[»]
2JA8X-ray3.80D1-221[»]
2R7ZX-ray3.80D1-221[»]
2R92X-ray3.80D1-221[»]
2R93X-ray4.00D1-221[»]
2VUMX-ray3.40D1-221[»]
3FKIX-ray3.88D1-221[»]
3H3VX-ray4.00E1-221[»]
3HOUX-ray3.20D/P1-221[»]
3HOVX-ray3.50D1-221[»]
3HOWX-ray3.60D1-221[»]
3HOXX-ray3.65D1-221[»]
3HOYX-ray3.40D1-221[»]
3HOZX-ray3.65D1-221[»]
3I4MX-ray3.70D1-221[»]
3I4NX-ray3.90D1-221[»]
3J0Kelectron microscopy36.00D1-221[»]
3J1Nelectron microscopy16.00D4-221[»]
3K1FX-ray4.30D1-221[»]
3PO2X-ray3.30D1-221[»]
3PO3X-ray3.30D1-221[»]
3QT1X-ray4.30D3-221[»]
4A3BX-ray3.50D1-221[»]
4A3CX-ray3.50D1-221[»]
4A3DX-ray3.40D1-221[»]
4A3EX-ray3.40D1-221[»]
4A3FX-ray3.50D1-221[»]
4A3GX-ray3.50D1-221[»]
4A3IX-ray3.80D1-221[»]
4A3JX-ray3.70D1-221[»]
4A3KX-ray3.50D1-221[»]
4A3LX-ray3.50D1-221[»]
4A3MX-ray3.90D1-221[»]
4A93X-ray3.40D1-221[»]
4BBRX-ray3.40D1-221[»]
4BBSX-ray3.60D1-221[»]
4BXXX-ray3.28D1-221[»]
4BXZX-ray4.80D1-221[»]
4BY1X-ray3.60D1-221[»]
4BY7X-ray3.15D1-221[»]
ProteinModelPortaliP20433.
SMRiP20433. Positions 1-221.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33617. 121 interactions.
DIPiDIP-55N.
IntActiP20433. 20 interactions.
MINTiMINT-538215.
STRINGi4932.YJL140W.

Proteomic databases

MaxQBiP20433.
PaxDbiP20433.
PeptideAtlasiP20433.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL140W; YJL140W; YJL140W.
GeneIDi853301.
KEGGisce:YJL140W.

Organism-specific databases

CYGDiYJL140w.
SGDiS000003676. RPB4.

Phylogenomic databases

eggNOGiCOG5250.
GeneTreeiENSGT00390000004912.
HOGENOMiHOG000195281.
InParanoidiP20433.
KOiK03012.
OMAiGTLECED.
OrthoDBiEOG7NW6NW.

Enzyme and pathway databases

BioCyciYEAST:G3O-31585-MONOMER.
ReactomeiREACT_273109. Formation of the Early Elongation Complex.
REACT_273984. Dual incision reaction in TC-NER.
REACT_277632. mRNA Capping.
REACT_294700. mRNA Splicing - Minor Pathway.
REACT_296055. RNA Polymerase II Promoter Escape.
REACT_300180. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_310018. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_315180. RNA Polymerase II Pre-transcription Events.
REACT_317581. Processing of Capped Intron-Containing Pre-mRNA.
REACT_322294. RNA Pol II CTD phosphorylation and interaction with CE.
REACT_331999. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_341636. Transcription-coupled NER (TC-NER).
REACT_345860. RNA Polymerase II Transcription Initiation.

Miscellaneous databases

EvolutionaryTraceiP20433.
NextBioi973621.
PROiP20433.

Gene expression databases

GenevestigatoriP20433.

Family and domain databases

InterProiIPR010997. HRDC-like.
IPR005574. RNA_pol_II_Rpb4.
IPR006590. RNA_pol_II_Rpb4_core.
[Graphical view]
PfamiPF03874. RNA_pol_Rpb4. 1 hit.
[Graphical view]
SMARTiSM00657. RPOL4c. 1 hit.
[Graphical view]
SUPFAMiSSF47819. SSF47819. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "RNA polymerase II subunit RPB4 is essential for high- and low-temperature yeast cell growth."
    Woychik N.A., Young R.A.
    Mol. Cell. Biol. 9:2854-2859(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The Saccharomyces cerevisiae RPB4 gene is tightly linked to the TIF2 gene."
    Foreman P.K., Davis R.W., Sachs A.B.
    Nucleic Acids Res. 19:2781-2781(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI."
    Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D.
    Yeast 12:787-797(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. "Two dissociable subunits of yeast RNA polymerase II stimulate the initiation of transcription at a promoter in vitro."
    Edwards A.M., Kane C.M., Young R.A., Kornberg R.D.
    J. Biol. Chem. 266:71-75(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RPB4-RPB7 COMPLEX.
  8. "Dissociable Rpb4-Rpb7 subassembly of RNA polymerase II binds to single-strand nucleic acid and mediates a post-recruitment step in transcription initiation."
    Orlicky S.M., Tran P.T., Sayre M.H., Edwards A.M.
    J. Biol. Chem. 276:10097-10102(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RPB4-RPB7 COMPLEX.
  9. "Rpb4, a non-essential subunit of core RNA polymerase II of Saccharomyces cerevisiae is important for activated transcription of a subset of genes."
    Pillai B., Sampath V., Sharma N., Sadhale P.
    J. Biol. Chem. 276:30641-30647(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Rpb4 and Rpb9 mediate subpathways of transcription-coupled DNA repair in Saccharomyces cerevisiae."
    Li S., Smerdon M.J.
    EMBO J. 21:5921-5929(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN DNA REPAIR.
  11. "Rpb4p, a subunit of RNA polymerase II, mediates mRNA export during stress."
    Farago M., Nahari T., Hammel C., Cole C.N., Choder M.
    Mol. Biol. Cell 14:2744-2755(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN MRNA EXPORT.
  12. "Structure and mechanism of RNA polymerase II CTD phosphatases."
    Kamenski T., Heilmeier S., Meinhart A., Cramer P.
    Mol. Cell 15:399-407(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RPB4-RPB7 COMPLEX.
  13. "The RNA polymerase II subunit Rpb4p mediates decay of a specific class of mRNAs."
    Lotan R., Bar-On V.G., Harel-Sharvit L., Duek L., Melamed D., Choder M.
    Genes Dev. 19:3004-3016(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN MRNA DECAY, SUBCELLULAR LOCATION, INTERACTION WITH PAT1 AND LSM2.
  14. "Nucleocytoplasmic shuttling of the Rpb4p and Rpb7p subunits of Saccharomyces cerevisiae RNA polymerase II by two pathways."
    Selitrennik M., Duek L., Lotan R., Choder M.
    Eukaryot. Cell 5:2092-2103(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  15. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-91 AND THR-92, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  16. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  17. "RNA polymerase II/TFIIF structure and conserved organization of the initiation complex."
    Chung W.H., Craighead J.L., Chang W.H., Ezeokonkwo C., Bareket-Samish A., Kornberg R.D., Asturias F.J.
    Mol. Cell 12:1003-1013(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: ELECTRON MICROSCOPY OF THE RNA POL II/TFIIF COMPLEX, INTERACTION WITH TFG1.
  18. "Architecture of the RNA polymerase II-TFIIS complex and implications for mRNA cleavage."
    Kettenberger H., Armache K.J., Cramer P.
    Cell 114:347-357(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.8 ANGSTROMS) OF THE RNA POL II COMPLEX IN COMPLEX WITH DST1.
  19. "Architecture of initiation-competent 12-subunit RNA polymerase II."
    Armache K.J., Kettenberger H., Cramer P.
    Proc. Natl. Acad. Sci. U.S.A. 100:6964-6968(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.2 ANGSTROMS) OF THE RNA POL II COMPLEX.
  20. "Complete RNA polymerase II elongation complex structure and its interactions with NTP and TFIIS."
    Kettenberger H., Armache K.J., Cramer P.
    Mol. Cell 16:955-965(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.5 ANGSTROMS) OF THE RNA POL II COMPLEX.
  21. "Structures of complete RNA polymerase II and its subcomplex, Rpb4/7."
    Armache K.J., Mitterweger S., Meinhart A., Cramer P.
    J. Biol. Chem. 280:7131-7134(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.8 ANGSTROMS) OF THE RNA POL II COMPLEX.
  22. "Structure of an RNA polymerase II-RNA inhibitor complex elucidates transcription regulation by noncoding RNAs."
    Kettenberger H., Eisenfuhr A., Brueckner F., Theis M., Famulok M., Cramer P.
    Nat. Struct. Mol. Biol. 13:44-48(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.8 ANGSTROMS) OF THE RNA POL II COMPLEX IN COMPLEX WITH INHIBITING NON-CODING RNA.
  23. "Phasing RNA polymerase II using intrinsically bound Zn atoms: an updated structural model."
    Meyer P.A., Ye P., Zhang M., Suh M.H., Fu J.
    Structure 14:973-982(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.15 ANGSTROMS) OF THE RNA POL II COMPLEX.

Entry informationi

Entry nameiRPB4_YEAST
AccessioniPrimary (citable) accession number: P20433
Secondary accession number(s): D6VW44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1991
Last sequence update: January 31, 1991
Last modified: March 31, 2015
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.