P20432 (GSTT1_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase 1-1
EC=2.5.1.18
EC=4.5.1.1

Alternative name(s):
DDT-dehydrochlorinase
GST class-theta

Gene names

Name:	GstD1
Synonyms:	GST, Gst1
ORF Names:	CG10045

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has DDT dehydrochlorinase activity. Ref.7
Catalytic activity	RX + glutathione = HX + R-S-glutathione. Ref.7 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane = 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride. Ref.7
Subunit structure	Homodimer. Ref.7
Miscellaneous	Has a specific activity toward 1-chloro-2,4-dinitrobenzene comparable to that for the mammalian glutathione S-transferases but did not have as broad a substrate specificity pattern. Has no GSH peroxidase activity.
Sequence similarities	Belongs to the GST superfamily. Theta family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Sequence caution	The sequence AAM52032.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Molecular function	Lyase Transferase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	glutathione metabolic process Inferred from direct assay PubMed 22082028. Source: FlyBase
Molecular_function	DDT-dehydrochlorinase activity Inferred from direct assay Ref.7. Source: FlyBase glutathione transferase activity Inferred from direct assay PubMed 12443531PubMed 22082028PubMed 3085676. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 209

209

Glutathione S-transferase 1-1

PRO_0000185947

Regions

Domain

1 – 81

81

GST N-terminal

Domain

87 – 208

122

GST C-terminal

Region

51 – 53

3

Glutathione binding

Region

65 – 67

3

Glutathione binding

Sites

Binding site

10

1

Glutathione

Secondary structure

1

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209

Helix Strand Turn

Details...

Beta strand

3 – 6

4

Helix

11 – 23

13

Beta strand

28 – 31

4

Helix

34 – 36

3

Helix

38 – 40

3

Helix

42 – 45

4

Beta strand

55 – 58

4

Beta strand

61 – 64

4

Helix

66 – 77

12

Beta strand

79 – 81

3

Helix

88 – 103

16

Helix

105 – 119

15

Helix

125 – 141

17

Turn

142 – 144

3

Beta strand

146 – 152

7

Helix

155 – 169

15

Helix

174 – 176

3

Helix

178 – 190

13

Helix

194 – 204

11

Helix

205 – 207

3

Sequences

	Sequence		Length	Mass (Da)
	P20432 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 4AD8E237CCF804F2	FASTA	209	23,866
	10 20 30 40 50 60 MVDFYYLPGS SPCRSVIMTA KAVGVELNKK LLNLQAGEHL KPEFLKINPQ HTIPTLVDNG 70 80 90 100 110 120 FALWESRAIQ VYLVEKYGKT DSLYPKCPKK RAVINQRLYF DMGTLYQSFA NYYYPQVFAK 130 140 150 160 170 180 APADPEAFKK IEAAFEFLNT FLEGQDYAAG DSLTVADIAL VATVSTFEVA KFEISKYANV 190 200 NRWYENAKKV TPGWEENWAG CLEFKKYFE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Drosophila glutathione S-transferase 1-1 shares a region of sequence homology with the maize glutathione S-transferase III." Toung Y.-P.S., Hsieh T.-S., Tu C.-P.D. Proc. Natl. Acad. Sci. U.S.A. 87:31-35(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-20. Strain: Oregon-R.
[2]	"The Drosophila glutathione S-transferase 1-1 is encoded by an intronless gene at 87B." Toung Y.-P.S., Hsieh T.-S., Tu C.-P.D. Biochem. Biophys. Res. Commun. 178:1205-1211(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]	"The glutathione S-transferase D genes. A divergently organized, intronless gene family in Drosophila melanogaster." Toung Y.-P.S., Hsieh T.-S., Tu C.-P.D. J. Biol. Chem. 268:9737-9746(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[5]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[6]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
[7]	"Recognition and detoxification of the insecticide DDT by Drosophila melanogaster glutathione S-transferase D1." Low W.Y., Feil S.C., Ng H.L., Gorman M.A., Morton C.J., Pyke J., McConville M.J., Bieri M., Mok Y.F., Robin C., Gooley P.R., Parker M.W., Batterham P. J. Mol. Biol. 399:358-366(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.13 ANGSTROMS) IN COMPLEX WITH GLUTATHIONE, FUNCTION AS DDT DEHYDROCHLORINASE, CATALYTIC ACTIVITY, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X14233 mRNA. Translation: CAA32449.1.
S51044 mRNA. Translation: AAA04220.1.
M97702 Genomic DNA. No translation available.
AE014297 Genomic DNA. Translation: AAF54786.1.
AE014297 Genomic DNA. Translation: ABC66168.1.
AY121705 mRNA. Translation: AAM52032.1. Different initiation.

PIR

XUFF11. A34798.

RefSeq

NP_001034042.1. NM_001038953.1.
NP_524326.1. NM_079602.4.

UniGene

Dm.2439.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3EIN	X-ray	1.13	A	1-209	[»]
3MAK	X-ray	1.80	A	1-209	[»]

ProteinModelPortal

P20432.

SMR

P20432. Positions 2-208.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-17237N.

IntAct

P20432. 2 interactions.

MINT

MINT-323021.

Proteomic databases

PaxDb

P20432.

PRIDE

P20432.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

FBtr0082607; FBpp0082077; FBgn0001149.
FBtr0100410; FBpp0099824; FBgn0001149.

GeneID

41503.

KEGG

dme:Dmel_CG10045.

Organism-specific databases

CTD

41503.

FlyBase

FBgn0001149. GstD1.

Phylogenomic databases

eggNOG

COG0625.

GeneTree

ENSGT00540000069741.

InParanoid

P20432.

KO

K00799.

OMA

LEGQEYA.

OrthoDB

EOG42RBQ8.

PhylomeDB

P20432.

Gene expression databases

Bgee

P20432.

GermOnline

CG10045. Drosophila melanogaster.

Family and domain databases

Gene3D

1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.

InterPro

IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR017933. Glutathione_S_Trfase/Cl_chnl_C.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]

Pfam

PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]

SUPFAM

SSF47616. GST_C_like. 1 hit.
SSF52833. Thiordxn-like_fd. 1 hit.

PROSITE

PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P20432.

GenomeRNAi

41503.

NextBio

824191.

Entry information

Entry name

GSTT1_DROME

Accession

Primary (citable) accession number: P20432
Secondary accession number(s): Q4JFI6 , Q8MR98, Q9TX88, Q9VG99

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	April 3, 2013
This is version 121 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project