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Protein

Glutathione S-transferase D1

Gene

GstD1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed:22082028, PubMed:20417639). Has DDT dehydrochlorinase activity (PubMed:20417639). May be involved in detoxification (PubMed:22082028).2 Publications

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.2 Publications
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane = 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride.1 Publication

Kineticsi

  1. KM=0.23 mM for glutathione1 Publication
  2. KM=0.45 mM for 1-chloro-2,4-dinitrobenzene1 Publication
  1. Vmax=84.2 µmol/min/mg enzyme with 1-chloro-2,4-dinitrobenzene as substrate1 Publication
  2. Vmax=7.51 µmol/min/mg enzyme with 4-hydroxy-2-nonenal as substrate1 Publication
  3. Vmax=77 nmol/min/mg enzyme with adrenochrome as substrate1 Publication
  4. Vmax=0.33 µmol/min/mg enzyme with phenethyl isothiocyanate as substrate1 Publication
  5. Vmax=0.517 µmol/min/mg enzyme with prostaglandin A2 as substrate1 Publication
  6. Vmax=0.012 µmol/min/mg enzyme with 5-hydroperoxyeicosatetraenoic acid as substrate1 Publication
  7. Vmax=0.22 µmol/min/mg enzyme with 2-hydroxyethyl disulfide as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10Glutathione1 Publication1

GO - Molecular functioni

  • DDT-dehydrochlorinase activity Source: FlyBase
  • glutathione peroxidase activity Source: GO_Central
  • glutathione transferase activity Source: FlyBase

GO - Biological processi

  • glutathione metabolic process Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Transferase

Keywords - Biological processi

Detoxification

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase D12 Publications (EC:2.5.1.182 Publications, EC:4.5.1.11 Publication)
Alternative name(s):
DDT-dehydrochlorinase1 Publication
Gene namesi
Name:GstD1Imported
Synonyms:GST, Gst1, GSTD1-1Imported
ORF Names:CG10045Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0001149. GstD1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001859471 – 209Glutathione S-transferase D1Add BLAST209

Proteomic databases

PaxDbiP20432.
PRIDEiP20432.

Expressioni

Gene expression databases

BgeeiFBgn0001149.
GenevisibleiP20432. DM.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi66612. 15 interactors.
DIPiDIP-17237N.
IntActiP20432. 4 interactors.
MINTiMINT-323021.
STRINGi7227.FBpp0099824.

Structurei

Secondary structure

1209
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 6Combined sources4
Helixi11 – 23Combined sources13
Beta strandi28 – 31Combined sources4
Helixi34 – 36Combined sources3
Helixi38 – 40Combined sources3
Helixi42 – 45Combined sources4
Beta strandi55 – 58Combined sources4
Beta strandi61 – 64Combined sources4
Helixi66 – 77Combined sources12
Beta strandi79 – 81Combined sources3
Helixi88 – 103Combined sources16
Helixi105 – 119Combined sources15
Helixi125 – 141Combined sources17
Turni142 – 144Combined sources3
Beta strandi146 – 152Combined sources7
Helixi155 – 169Combined sources15
Helixi174 – 176Combined sources3
Helixi178 – 190Combined sources13
Helixi194 – 204Combined sources11
Helixi205 – 207Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EINX-ray1.13A1-209[»]
3MAKX-ray1.80A1-209[»]
ProteinModelPortaliP20432.
SMRiP20432.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20432.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 81GST N-terminalAdd BLAST81
Domaini87 – 208GST C-terminalAdd BLAST122

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni51 – 53Glutathione binding3
Regioni65 – 67Glutathione binding3

Sequence similaritiesi

Belongs to the GST superfamily. Delta family.1 Publication
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
COG0625. LUCA.
GeneTreeiENSGT00540000069741.
InParanoidiP20432.
KOiK00799.
OMAiFARIFTT.
OrthoDBiEOG091G0MS3.
PhylomeDBiP20432.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20432-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDFYYLPGS SPCRSVIMTA KAVGVELNKK LLNLQAGEHL KPEFLKINPQ
60 70 80 90 100
HTIPTLVDNG FALWESRAIQ VYLVEKYGKT DSLYPKCPKK RAVINQRLYF
110 120 130 140 150
DMGTLYQSFA NYYYPQVFAK APADPEAFKK IEAAFEFLNT FLEGQDYAAG
160 170 180 190 200
DSLTVADIAL VATVSTFEVA KFEISKYANV NRWYENAKKV TPGWEENWAG

CLEFKKYFE
Length:209
Mass (Da):23,866
Last modified:February 1, 1991 - v1
Checksum:i4AD8E237CCF804F2
GO

Sequence cautioni

The sequence AAM52032 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14233 mRNA. Translation: CAA32449.1.
S51044 mRNA. Translation: AAA04220.1.
M97702 Genomic DNA. No translation available.
AE014297 Genomic DNA. Translation: AAF54786.1.
AE014297 Genomic DNA. Translation: ABC66168.1.
AY121705 mRNA. Translation: AAM52032.1. Different initiation.
PIRiA34798. XUFF11.
RefSeqiNP_001034042.1. NM_001038953.2.
NP_524326.1. NM_079602.5.
UniGeneiDm.2439.

Genome annotation databases

EnsemblMetazoaiFBtr0082607; FBpp0082077; FBgn0001149.
FBtr0100410; FBpp0099824; FBgn0001149.
GeneIDi41503.
KEGGidme:Dmel_CG10045.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14233 mRNA. Translation: CAA32449.1.
S51044 mRNA. Translation: AAA04220.1.
M97702 Genomic DNA. No translation available.
AE014297 Genomic DNA. Translation: AAF54786.1.
AE014297 Genomic DNA. Translation: ABC66168.1.
AY121705 mRNA. Translation: AAM52032.1. Different initiation.
PIRiA34798. XUFF11.
RefSeqiNP_001034042.1. NM_001038953.2.
NP_524326.1. NM_079602.5.
UniGeneiDm.2439.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EINX-ray1.13A1-209[»]
3MAKX-ray1.80A1-209[»]
ProteinModelPortaliP20432.
SMRiP20432.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi66612. 15 interactors.
DIPiDIP-17237N.
IntActiP20432. 4 interactors.
MINTiMINT-323021.
STRINGi7227.FBpp0099824.

Proteomic databases

PaxDbiP20432.
PRIDEiP20432.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0082607; FBpp0082077; FBgn0001149.
FBtr0100410; FBpp0099824; FBgn0001149.
GeneIDi41503.
KEGGidme:Dmel_CG10045.

Organism-specific databases

CTDi41503.
FlyBaseiFBgn0001149. GstD1.

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
COG0625. LUCA.
GeneTreeiENSGT00540000069741.
InParanoidiP20432.
KOiK00799.
OMAiFARIFTT.
OrthoDBiEOG091G0MS3.
PhylomeDBiP20432.

Miscellaneous databases

ChiTaRSiGstS1. fly.
EvolutionaryTraceiP20432.
GenomeRNAii41503.
PROiP20432.

Gene expression databases

BgeeiFBgn0001149.
GenevisibleiP20432. DM.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTD1_DROME
AccessioniPrimary (citable) accession number: P20432
Secondary accession number(s): Q4JFI6
, Q8MR98, Q9TX88, Q9VG99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 2, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

Has a specific activity toward 1-chloro-2,4-dinitrobenzene comparable to that for the mammalian glutathione S-transferases but did not have as broad a substrate specificity pattern. Has no GSH peroxidase activity.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.