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Protein

ATPase 3, plasma membrane-type

Gene

AHA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The plasma membrane H+ ATPase of plants and fungi generates a proton gradient that drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei330 – 33014-aspartylphosphate intermediateBy similarity
Metal bindingi589 – 5891MagnesiumBy similarity
Metal bindingi593 – 5931MagnesiumBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • hydrogen-exporting ATPase activity, phosphorylative mechanism Source: UniProtKB
  • magnesium ion binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G57350-MONOMER.
ARA:GQT-1212-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase 3, plasma membrane-type (EC:3.6.3.6)
Alternative name(s):
Proton pump 3
Gene namesi
Name:AHA3
Ordered Locus Names:At5g57350
ORF Names:MJB24.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G57350.

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6262CytoplasmicSequence analysisAdd
BLAST
Transmembranei63 – 8220Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini83 – 9412ExtracellularSequence analysisAdd
BLAST
Transmembranei95 – 11521Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini116 – 244129CytoplasmicSequence analysisAdd
BLAST
Transmembranei245 – 26521Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini266 – 2749ExtracellularSequence analysis
Transmembranei275 – 29218Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini293 – 644352CytoplasmicSequence analysisAdd
BLAST
Transmembranei645 – 66622Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini667 – 6715ExtracellularSequence analysis
Transmembranei672 – 69423Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini695 – 71016CytoplasmicSequence analysisAdd
BLAST
Transmembranei711 – 73121Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini732 – 75221ExtracellularSequence analysisAdd
BLAST
Transmembranei753 – 77321Helical; Name=8Sequence analysisAdd
BLAST
Topological domaini774 – 78512CytoplasmicSequence analysisAdd
BLAST
Transmembranei786 – 80621Helical; Name=9Sequence analysisAdd
BLAST
Topological domaini807 – 8148ExtracellularSequence analysis
Transmembranei815 – 83521Helical; Name=10Sequence analysisAdd
BLAST
Topological domaini836 – 949114CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB
  • integral component of plasma membrane Source: GO_Central
  • intracellular membrane-bounded organelle Source: GO_Central
  • membrane Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi947 – 9471Y → A: Impaired fusicoccin- (FC) dependent activation by 14-3-3 protein. 1 Publication
Mutagenesisi948 – 9481T → A: Impaired fusicoccin- (FC) dependent activation by 14-3-3 protein. 1 Publication
Mutagenesisi949 – 9491V → A: Impaired fusicoccin- (FC) dependent activation by 14-3-3 protein. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 949949ATPase 3, plasma membrane-typePRO_0000046276Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei882 – 8821PhosphothreonineCombined sources
Modified residuei932 – 9321PhosphoserineBy similarity
Modified residuei948 – 9481Phosphothreonine1 Publication

Post-translational modificationi

Phosphorylation level varies significantly during early response to bacterial elicitor (e.g. fusicoccin FC).1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP20431.
PRIDEiP20431.

PTM databases

iPTMnetiP20431.

Expressioni

Tissue specificityi

Found predominantly in phloem cells of leaves, stems, roots and flowers.

Gene expression databases

GenevisibleiP20431. AT.

Interactioni

Subunit structurei

Binds to 14-3-3 proteins. The binding is induced by phosphorylation of Thr-948. Binding to 14-3-3 proteins activates the H+-ATPase.

Protein-protein interaction databases

BioGridi21085. 4 interactions.
MINTiMINT-8063022.
STRINGi3702.AT5G57350.1.

Structurei

3D structure databases

ProteinModelPortaliP20431.
SMRiP20431. Positions 13-845, 901-943.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni947 – 9493Interaction with 14-3-3 proteins

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0205. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000160005.
InParanoidiP20431.
KOiK01535.
OMAiHRKYREG.
OrthoDBiEOG093602AD.
PhylomeDBiP20431.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006534. P-type_ATPase_IIIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20431-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGLEDIVN ENVDLEKIPI EEVFQQLKCS REGLSGAEGE NRLQIFGPNK
60 70 80 90 100
LEEKKESKLL KFLGFMWNPL SWVMEAAAIM AIALANGGGK PPDWQDFVGI
110 120 130 140 150
VCLLVINSTI SFVEENNAGN AAAALMAGLA PKTKVLRDGK WSEQEASILV
160 170 180 190 200
PGDIVSIKLG DIIPADARLL EGDPLKVDQS ALTGESLPAT KGPGEEVFSG
210 220 230 240 250
STCKQGEIEA VVIATGVHTF FGKAAHLVDS TNQVGHFQKV LTAIGNFCIC
260 270 280 290 300
SIAVGIAIEI VVMYPIQRRH YRDGIDNLLV LLIGGIPIAM PTVLSVTMAI
310 320 330 340 350
GSHKLSQQGA ITKRMTAIEE MAGMDVLCSD KTGTLTLNKL SVDKNLIEVY
360 370 380 390 400
CKGVEKDEVL LFAARASRVE NQDAIDAAMV GMLADPKEAR AGIREIHFLP
410 420 430 440 450
FNPVDKRTAL TFIDSNGNWH RVSKGAPEQI LDLCNARADL RKRVHSTIDK
460 470 480 490 500
YAERGLRSLA VSRQTVPEKT KESSGSPWEF VGVLPLFDPP RHDSAETIRR
510 520 530 540 550
ALDLGVNVKM ITGDQLAIAK ETGRRLGMGS NMYPSSSLLG KHKDEAMAHI
560 570 580 590 600
PVEDLIEKAD GFAGVFPEHK YEIVKKLQER KHICGMTGDG VNDAPALKKA
610 620 630 640 650
DIGIAVADAT DAARGASDIV LTEPGLSVII SAVLTSRAIF QRMKNYTIYA
660 670 680 690 700
VSITIRIVFG FMLIALIWKF DFSPFMVLII AILNDGTIMT ISKDRVKPSP
710 720 730 740 750
TPDSWKLKEI FATGVVLGGY MAIMTVVFFW AAYKTDFFPR TFHVRDLRGS
760 770 780 790 800
EHEMMSALYL QVSIVSQALI FVTRSRSWSF TERPGYFLLI AFWVAQLIAT
810 820 830 840 850
AIAVYGNWEF ARIKGIGWGW AGVIWLYSIV FYFPLDIMKF AIRYILAGTA
860 870 880 890 900
WKNIIDNRTA FTTKQNYGIE EREAQWAHAQ RTLHGLQNTE TANVVPERGG
910 920 930 940
YRELSEIANQ AKRRAEIARL RELHTLKGHV ESVVKLKGLD IETAGHYTV
Length:949
Mass (Da):104,450
Last modified:January 23, 2007 - v2
Checksum:i3F88C87D849A4001
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04737 Genomic DNA. Translation: AAA32750.1.
AB019233, AB016891 Genomic DNA. Translation: BAB09963.1.
CP002688 Genomic DNA. Translation: AED96889.1.
CP002688 Genomic DNA. Translation: AED96890.1.
AY072153 mRNA. Translation: AAL59975.1.
X60115 Genomic DNA. Translation: CAA42714.1.
PIRiA33698. PXMUP3.
RefSeqiNP_001190559.1. NM_001203630.1.
NP_200545.1. NM_125118.2.
UniGeneiAt.290.

Genome annotation databases

EnsemblPlantsiAT5G57350.1; AT5G57350.1; AT5G57350.
AT5G57350.2; AT5G57350.2; AT5G57350.
GeneIDi835841.
GrameneiAT5G57350.1; AT5G57350.1; AT5G57350.
AT5G57350.2; AT5G57350.2; AT5G57350.
KEGGiath:AT5G57350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04737 Genomic DNA. Translation: AAA32750.1.
AB019233, AB016891 Genomic DNA. Translation: BAB09963.1.
CP002688 Genomic DNA. Translation: AED96889.1.
CP002688 Genomic DNA. Translation: AED96890.1.
AY072153 mRNA. Translation: AAL59975.1.
X60115 Genomic DNA. Translation: CAA42714.1.
PIRiA33698. PXMUP3.
RefSeqiNP_001190559.1. NM_001203630.1.
NP_200545.1. NM_125118.2.
UniGeneiAt.290.

3D structure databases

ProteinModelPortaliP20431.
SMRiP20431. Positions 13-845, 901-943.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21085. 4 interactions.
MINTiMINT-8063022.
STRINGi3702.AT5G57350.1.

PTM databases

iPTMnetiP20431.

Proteomic databases

PaxDbiP20431.
PRIDEiP20431.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G57350.1; AT5G57350.1; AT5G57350.
AT5G57350.2; AT5G57350.2; AT5G57350.
GeneIDi835841.
GrameneiAT5G57350.1; AT5G57350.1; AT5G57350.
AT5G57350.2; AT5G57350.2; AT5G57350.
KEGGiath:AT5G57350.

Organism-specific databases

TAIRiAT5G57350.

Phylogenomic databases

eggNOGiKOG0205. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000160005.
InParanoidiP20431.
KOiK01535.
OMAiHRKYREG.
OrthoDBiEOG093602AD.
PhylomeDBiP20431.

Enzyme and pathway databases

BioCyciARA:AT5G57350-MONOMER.
ARA:GQT-1212-MONOMER.

Miscellaneous databases

PROiP20431.

Gene expression databases

GenevisibleiP20431. AT.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006534. P-type_ATPase_IIIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPMA3_ARATH
AccessioniPrimary (citable) accession number: P20431
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.