P20430 (L_TACVF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L
EC=2.7.7.48

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Gene names

Name:

L

Organism

Tacaribe virus (strain Franze-Fernandez) (TCRV)

Taxonomic identifier

928313 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Arenaviridae › Arenavirus › New world arenaviruses

Virus host

Artibeus (neotropical fruit bats) [TaxID: 9416]

Protein attributes

Sequence length	2210 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N. Interacts with protein Z; this interaction inhibits viral transcription and replication By similarity. Ref.2
Subcellular location	Virion. Host cytoplasm.
Sequence similarities	Belongs to the arenaviridae RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Cap snatching RNA replication
Cellular component	Host cytoplasm Virion
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2210	2210	RNA-directed RNA polymerase L		PRO_0000079200
Regions
	Domain	1172 – 1368	197	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	P20430 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: EACF01C32761024F	FASTA	2,210	252,232
	10 20 30 40 50 60 MDETVSELKD LVRKHIPNRH EFAHQKDAFL SHCHSGSLLQ EGFKLLSNLV ELESCESHAC 70 80 90 100 110 120 HLNTCQKYVD VILSDHGIPC PTLPKVIPDG FKLTGKTLIL LETFVRVNPE EFERKWKSDM 130 140 150 160 170 180 TKLLNLKQDL LRSGITLVPV VDGRTNYSNR FTPEWVVERI RWLLIEILRK SRSSAEIDIE 190 200 210 220 230 240 DQEYQRLIHS LSNVRNQSLG FENIECLKRN LLEYDDRLAK SLFVGVKGDV RESVIREELM 250 260 270 280 290 300 KLRLWYKKEV FDKNLGKFRI TNRSELLNNL IRLGKHEDNT TSDCPFCVNK FMDIIYSLTF 310 320 330 340 350 360 TALKRQDREK SNSELDQYVV CPHEKAYLGV LSICNKIKGL KVFNTRRNTL LFLDLIMVNF 370 380 390 400 410 420 LDDLFTAKPE ALDSLRRSGL ILGQMVTLVN DRALDFLEAV KLIKKKIETN VKWVENCSKI 430 440 450 460 470 480 LRRSQQDIWS QISVWARYPD LSKLISIAQT ISSDRPIMRY SAGGNFNTEC KHKTFHMMSD 490 500 510 520 530 540 AEQVEAFKIL SSVSLSLINS MKTSFSSRLL INEKEYSRYF GNVRLRECYQ QRFFLTDGLI 550 560 570 580 590 600 VILFYQKTGE RSGCYSIYTC EDGVLVEKGS FYCDPKRFFL PIFSQEVLVE MCDEMTTWLD 610 620 630 640 650 660 FNSDLMVISK EKLRLLLLSI LCAPSKRNQV FLQGLRYFLM AYSNQFHHVD LLSKLKVECM 670 680 690 700 710 720 SGSEVIVQRL AVDLFQCLLG EGVDSDPYFA RRFKYLLNVS YLCHLITKET PDRLTDQIKC 730 740 750 760 770 780 FEKFIEPKID FNCVIVNPSL NGQLTEAQEG MMLDGLDKFY SKTLKDCSDT KLPGVSNELL 790 800 810 820 830 840 SYCISLFNKG KLKVTGELKN DPFKPNITST ALDLSSNKSV VVPKLDELGN VLSVYDREKM 850 860 870 880 890 900 ISSCVSSMAE RFKTKGRYNI DPSTLDYLIL KNLTGLVSIG SKTQRDCEEL SMMFEGLTEE 910 920 930 940 950 960 QAEAFNDIKN SVQLAMVKMK DSKSGDVNLS PNQKEGRVKS STGTLEELWG PFGIMREIRT 970 980 990 1000 1010 1020 EVSLHEVKDF DPDVLASDLY KELCDVVYYS SSKPEYFLER PLEVCPLGLL LKNLTTSAYF 1030 1040 1050 1060 1070 1080 DEEYFECFKY LLIQGHYDQK LGSYEHRSRS RLGFTNEALR VKDEVRLSMR ESNSEAIADK 1090 1100 1110 1120 1130 1140 LDRSYFTNAA LRNLCFYSDD SPTEFTSISS NNGNLKFGLS YKEQVGSNRE LYVGDLNTKL 1150 1160 1170 1180 1190 1200 ITRLVEDFAE AVGSSMRYTC LSSEKEFDRA ICDMKLAVNN GDLSCSLDHS KWGPTMSPAL 1210 1220 1230 1240 1250 1260 FLTFLQFLEL RTPKERNIIN LEPVLNVLRW HLHKVIEVPV NVAEAYCTGN LKRSLGLMGC 1270 1280 1290 1300 1310 1320 GSSSVGEEFF HQFMPVQGEI PSHIMSVLDM GQGILHNMSD LYGLITEQFL NYVLDLLYDV 1330 1340 1350 1360 1370 1380 IPTSYTSSDD QVTLIKLPCA SDDNQVNDEW LEMLCFHEYL SSKLNKFVSP KSVAGTFVAE 1390 1400 1410 1420 1430 1440 FKSRFFVMGE ETPLLTQFVA AALHNVKCKT PTQLSETIDT ICDQCVANGV SVQIVSKISQ 1450 1460 1470 1480 1490 1500 RVNQLIKYSG FKETPFGAVE KQDVKDWVDG TRGYRLQRKI ESIFSDDEMT GFIRSCAKRV 1510 1520 1530 1540 1550 1560 FNDIKRGKVF EENLISLIGR DGDDALVGFL RYSSCSEQDI MRALGFRWVN LSSFGDLRLV 1570 1580 1590 1600 1610 1620 LRTKLMTSRR VLEREEVPTL IKTLQSRLSR NFTKGVKKIL AESINKSAFQ SSVASGFIGF 1630 1640 1650 1660 1670 1680 CKSIGSKCVR DGEGGFLYIK DIYTKVKPCL CEVCNMKRGV IYCRPSLEKI EKFSKPILWD 1690 1700 1710 1720 1730 1740 YFSLVLTNAC EIGEWVFSSV KEPQIPVVLS NRNLFWAVKP RIVRQLEDQL GMNHVLYSIR 1750 1760 1770 1780 1790 1800 KNYPKLFDEH LSPFMSDLQV NRTLDGRKLK FLDVCIALDL MNENLGIVSH LLKARDNSVY 1810 1820 1830 1840 1850 1860 IVKQSDCAMA HVRQSDYVDK EVGLSPQQVC YNFMVQIILS SMVNPLVMST SCLKSFFWFN 1870 1880 1890 1900 1910 1920 EVLELEDDGQ IELGELTDFT FLVRDQKISR AMFIEDIAMG YVISNLEDVR LYIDKITIGE 1930 1940 1950 1960 1970 1980 QPLAPGRHIN DLLDLLGNFD DHEDCDLRFL IQVEHSRTST KYRFKRKMTY SFSVTCVSKV 1990 2000 2010 2020 2030 2040 IDLKEASVEL QVVDVTQSVS GSGGSHLLLD GVSMIAGLPI FTGQGTFNMA SLMMDADLVE 2050 2060 2070 2080 2090 2100 TNDNLILTDV RFSFGGFLSE LSDKYAYTLN GPVDQGEPLV LRDGHFFMGT EKVSTYRVEL 2110 2120 2130 2140 2150 2160 TGDIIVKAIG ALDDPEDVNA LLNQLWPYLK STAQVMLFQQ EDFVLVYDLH RSGLIRSLEL 2170 2180 2190 2200 2210 IGDWVEFVNF KVAYSKSLKD LVVSDNQGSL RLRGIMCRPL ARRNTVEDIE

References

[1]	"Tacaribe virus L gene encodes a protein of 2210 amino acid residues." Iapalucci S., Lopez R., Rey O., Lopez N., Franze-Fernandez M.T., Cohen G.N., Lucero M., Ochoa A., Zakin M.M. Virology 170:40-47(1989) [PubMed: 2718387] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Tacaribe virus Z protein interacts with the L polymerase protein to inhibit viral RNA synthesis." Jacamo R., Lopez N., Wilda M., Franze-Fernandez M.T. J. Virol. 77:10383-10393(2003) [PubMed: 12970423] [Abstract] Cited for: INTERACTION WITH PROTEINS Z AND N.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04340 Genomic RNA. Translation: AAA47901.1.
PIR	RRXPTV. A31468.
RefSeq	NP_694848.1. NC_004292.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	956595.
Family and domain databases
InterPro	IPR014386. RNA-dir_pol_arenavirus. IPR007099. RNA-dir_pol_NSvirus. IPR010453. RNA_pol_arenavir. [Graphical view]
Pfam	PF06317. Arena_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000836. L_ArenaV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_TACVF

Accession

Primary (citable) accession number: P20430

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	September 21, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program