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Protein

Myogenin

Gene

Myog

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processCell cycle, Differentiation, Myogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-RNO-375170. CDO in myogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Myogenin
Gene namesi
Name:Myog
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi620432. Myog.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi77S → A: Reduces phosphorylation. 1 Publication1
Mutagenesisi79S → A: Reduces phosphorylation. 1 Publication1
Mutagenesisi87T → A: Reduces phosphorylation and inhibits DNA-binding and transcriptional activation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001273781 – 287MyogeninAdd BLAST287

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei77Phosphoserine; by CaMK2G1 Publication1
Modified residuei79Phosphoserine; by CaMK2G1 Publication1
Modified residuei87Phosphothreonine; by CaMK2G1 Publication1

Post-translational modificationi

Phosphorylated by CAMK2G on threonine and serine amino acids in a muscle activity-dependent manner. Phosphorylation of Thr-87 impairs both DNA-binding and trans-activation functions in contracting muscles.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP20428.
PRIDEiP20428.

PTM databases

iPTMnetiP20428.

Expressioni

Tissue specificityi

Expressed in muscle (at protein level).

Inductioni

Up-regulated in denerved muscles cells. Down-regulated in soleus muscle in an electrical activity-dependent manner.1 Publication

Gene expression databases

BgeeiENSRNOG00000030743.
ExpressionAtlasiP20428. baseline and differential.
GenevisibleiP20428. RN.

Interactioni

Subunit structurei

Homodimer and heterodimer with E12; heterodimerization enhances MYOG DNA-binding and transcriptional activities. Interacts with SMARCA4/BRG1/BAF190A. Interacts (via C-terminal region) with SSRP1 and SUPT16H; the interaction is indicative of an interaction with the FACT complex (By similarity). Interacts with CSRP3.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-52N.
STRINGi10116.ENSRNOP00000039817.

Structurei

3D structure databases

ProteinModelPortaliP20428.
SMRiP20428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 132bHLHPROSITE-ProRule annotationAdd BLAST52

Phylogenomic databases

eggNOGiKOG3960. Eukaryota.
ENOG4111SED. LUCA.
GeneTreeiENSGT00530000063004.
HOGENOMiHOG000234799.
InParanoidiP20428.
KOiK18483.
OMAiPCPTEIV.
OrthoDBiEOG091G0GKD.
PhylomeDBiP20428.
TreeFamiTF316344.

Family and domain databases

CDDicd00083. HLH. 1 hit.
Gene3Di4.10.280.10. 1 hit.
InterProiView protein in InterPro
IPR002546. Basic.
IPR011598. bHLH_dom.
IPR036638. HLH_DNA-bd_sf.
PfamiView protein in Pfam
PF01586. Basic. 1 hit.
PF00010. HLH. 1 hit.
SMARTiView protein in SMART
SM00520. BASIC. 1 hit.
SM00353. HLH. 1 hit.
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiView protein in PROSITE
PS50888. BHLH. 1 hit.

Sequencei

Sequence statusi: Complete.

P20428-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELYETSPYF YQEPHFYDGE NYLPVHLQGF EPPGYERTEL SLSPEARGPL
60 70 80 90 100
EEKGLGTPEH CPGQCLPWAC KVCKRKSVSV DRRRAATLRE KRRLKKVNEA
110 120 130 140 150
FEALKRSTLL NPNQRLPKVE ILRSAIQYIE RLQALLSSLN QEERDLRYRG
160 170 180 190 200
GGGPSRWYPV NATPTAPPAV RSGAMHWSLV PTQEIICSQL TLQVPTTCTP
210 220 230 240 250
LRPSWTASRW RICLSPSQMK PCPTEIVCQA GCAWEPLSWC QTPPLLQQGP
260 270 280
FKWGCPGAQK TALGCHKPDY PPSIHIRLTP SPAREFN
Length:287
Mass (Da):32,503
Last modified:February 1, 1991 - v1
Checksum:iBE454E59B1464B40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24393 mRNA. Translation: AAA41662.1.
PIRiA31876.
RefSeqiNP_058811.2. NM_017115.2.
UniGeneiRn.9465.

Genome annotation databases

EnsembliENSRNOT00000087240; ENSRNOP00000073308; ENSRNOG00000030743.
GeneIDi29148.
KEGGirno:29148.
UCSCiRGD:620432. rat.

Similar proteinsi

Entry informationi

Entry nameiMYOG_RAT
AccessioniPrimary (citable) accession number: P20428
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 22, 2017
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome