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Protein

Myogenin

Gene

Myog

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Cell cycle, Differentiation, Myogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-375170. CDO in myogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Myogenin
Gene namesi
Name:Myog
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi620432. Myog.

Subcellular locationi

  • Nucleus

  • Note: Recruited to late myogenic gene promoter regulatory sequences with SMARCA4/BRG1/BAF190A and SWI/SNF chromatin-remodeling enzymes to promote chromatin-remodeling and transcription initiation in developing embryos.By similarity

GO - Cellular componenti

  • nucleus Source: UniProtKB
  • protein-DNA complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi77 – 771S → A: Reduces phosphorylation. 1 Publication
Mutagenesisi79 – 791S → A: Reduces phosphorylation. 1 Publication
Mutagenesisi87 – 871T → A: Reduces phosphorylation and inhibits DNA-binding and transcriptional activation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 287287MyogeninPRO_0000127378Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei77 – 771Phosphoserine; by CaMK2G1 Publication
Modified residuei79 – 791Phosphoserine; by CaMK2G1 Publication
Modified residuei87 – 871Phosphothreonine; by CaMK2G1 Publication

Post-translational modificationi

Phosphorylated by CAMK2G on threonine and serine amino acids in a muscle activity-dependent manner. Phosphorylation of Thr-87 impairs both DNA-binding and trans-activation functions in contracting muscles.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP20428.
PRIDEiP20428.

PTM databases

iPTMnetiP20428.

Expressioni

Tissue specificityi

Expressed in muscle (at protein level).

Inductioni

Up-regulated in denerved muscles cells. Down-regulated in soleus muscle in an electrical activity-dependent manner.1 Publication

Gene expression databases

ExpressionAtlasiP20428. baseline and differential.
GenevisibleiP20428. RN.

Interactioni

Subunit structurei

Homodimer and heterodimer with E12; heterodimerization enhances MYOG DNA-binding and transcriptional activities. Interacts with SMARCA4/BRG1/BAF190A. Interacts (via C-terminal region) with SSRP1 and SUPT16H; the interaction is indicative of an interaction with the FACT complex (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-52N.
STRINGi10116.ENSRNOP00000039817.

Structurei

3D structure databases

ProteinModelPortaliP20428.
SMRiP20428. Positions 74-136.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 13252bHLHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3960. Eukaryota.
ENOG4111SED. LUCA.
GeneTreeiENSGT00530000063004.
HOGENOMiHOG000234799.
InParanoidiP20428.
KOiK18483.
PhylomeDBiP20428.
TreeFamiTF316344.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR002546. Basic.
IPR011598. bHLH_dom.
[Graphical view]
PfamiPF01586. Basic. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00520. BASIC. 1 hit.
SM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20428-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELYETSPYF YQEPHFYDGE NYLPVHLQGF EPPGYERTEL SLSPEARGPL
60 70 80 90 100
EEKGLGTPEH CPGQCLPWAC KVCKRKSVSV DRRRAATLRE KRRLKKVNEA
110 120 130 140 150
FEALKRSTLL NPNQRLPKVE ILRSAIQYIE RLQALLSSLN QEERDLRYRG
160 170 180 190 200
GGGPSRWYPV NATPTAPPAV RSGAMHWSLV PTQEIICSQL TLQVPTTCTP
210 220 230 240 250
LRPSWTASRW RICLSPSQMK PCPTEIVCQA GCAWEPLSWC QTPPLLQQGP
260 270 280
FKWGCPGAQK TALGCHKPDY PPSIHIRLTP SPAREFN
Length:287
Mass (Da):32,503
Last modified:February 1, 1991 - v1
Checksum:iBE454E59B1464B40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24393 mRNA. Translation: AAA41662.1.
PIRiA31876.
RefSeqiNP_058811.2. NM_017115.2.
UniGeneiRn.9465.

Genome annotation databases

EnsembliENSRNOT00000087240; ENSRNOP00000073308; ENSRNOG00000030743.
GeneIDi29148.
KEGGirno:29148.
UCSCiRGD:620432. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24393 mRNA. Translation: AAA41662.1.
PIRiA31876.
RefSeqiNP_058811.2. NM_017115.2.
UniGeneiRn.9465.

3D structure databases

ProteinModelPortaliP20428.
SMRiP20428. Positions 74-136.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-52N.
STRINGi10116.ENSRNOP00000039817.

PTM databases

iPTMnetiP20428.

Proteomic databases

PaxDbiP20428.
PRIDEiP20428.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000087240; ENSRNOP00000073308; ENSRNOG00000030743.
GeneIDi29148.
KEGGirno:29148.
UCSCiRGD:620432. rat.

Organism-specific databases

CTDi4656.
RGDi620432. Myog.

Phylogenomic databases

eggNOGiKOG3960. Eukaryota.
ENOG4111SED. LUCA.
GeneTreeiENSGT00530000063004.
HOGENOMiHOG000234799.
InParanoidiP20428.
KOiK18483.
PhylomeDBiP20428.
TreeFamiTF316344.

Enzyme and pathway databases

ReactomeiR-RNO-375170. CDO in myogenesis.

Miscellaneous databases

NextBioi608147.
PROiP20428.

Gene expression databases

ExpressionAtlasiP20428. baseline and differential.
GenevisibleiP20428. RN.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR002546. Basic.
IPR011598. bHLH_dom.
[Graphical view]
PfamiPF01586. Basic. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00520. BASIC. 1 hit.
SM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD."
    Wright W.E., Sassoon D.A., Lin V.K.
    Cell 56:607-617(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Myogenin and MyoD join a family of skeletal muscle genes regulated by electrical activity."
    Eftimie R., Brenner H.R., Buonanno A.
    Proc. Natl. Acad. Sci. U.S.A. 88:1349-1353(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  3. "CaM kinase II-dependent phosphorylation of myogenin contributes to activity-dependent suppression of nAChR gene expression in developing rat myotubes."
    Tang H., Macpherson P., Argetsinger L.S., Cieslak D., Suhr S.T., Carter-Su C., Goldman D.
    Cell. Signal. 16:551-563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION AT SER-77; SER-79 AND THR-87, DNA-BINDING, MUTAGENESIS OF SER-77; SER-79 AND THR-87.

Entry informationi

Entry nameiMYOG_RAT
AccessioniPrimary (citable) accession number: P20428
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: May 11, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.