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Protein

Signal recognition particle subunit SRP54

Gene

SRP54

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Signal-recognition-particle (SRP) assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum (ER) membrane. SRP is required for the cotranslational protein translocation for ER import and preferentially recognizes strongly hydrophobic signal sequences. It is involved in targeting the nascent chain-ribosome (RNC) complex to the ER and is proposed to participate in the arrest of nascent chain elongation during membrane targeting. SRP54 binds to the signal sequence of presecretory protein when they emerge from the ribosomes. SRP54 interacts with the scR1 RNA and mediates the association of the resulting SRP-RNC complex with the signal recognition particle receptor (SR) via its alpha subunit SRP101. Both, SRP54 and SRP101, are locked in their GTP bound forms in the SRP-RNC-SR complex, which dissociates upon transferring the signal sequence to the protein-conducting channel (translocon). After signal sequence transfer, SRP54 and SRP101 act as reciprocal GTPAse-activating proteins (GAPs), thereby resolving their association.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi116 – 123GTPBy similarity8
Nucleotide bindingi198 – 202GTPBy similarity5
Nucleotide bindingi256 – 259GTPBy similarity4

GO - Molecular functioni

  • 7S RNA binding Source: SGD
  • endoplasmic reticulum signal peptide binding Source: SGD
  • GTPase activator activity Source: UniProtKB-KW
  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • SRP-dependent cotranslational protein targeting to membrane Source: SGD
  • SRP-dependent cotranslational protein targeting to membrane, translocation Source: SGD

Keywordsi

Molecular functionGTPase activation, Ribonucleoprotein, RNA-binding
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34231-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle subunit SRP54
Alternative name(s):
Signal recognition particle 54 kDa protein homolog
Gene namesi
Name:SRP54
Synonyms:SRH1
Ordered Locus Names:YPR088C
ORF Names:P9513.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR088C.
SGDiS000006292. SRP54.

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Signal recognition particle

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001012031 – 541Signal recognition particle subunit SRP54Add BLAST541

Proteomic databases

MaxQBiP20424.
PRIDEiP20424.

PTM databases

iPTMnetiP20424.

Interactioni

Subunit structurei

Fungal signal recognition particle (SRP) complex consists of a 7S RNA molecule (scR1) and at least six protein subunits: SRP72, SRP68, SRP54, SEC65, SRP21 and SRP14. SRP54 interacts with SRP101.1 Publication

Protein-protein interaction databases

BioGridi36258. 267 interactors.
DIPiDIP-2943N.
IntActiP20424. 27 interactors.
MINTiMINT-484768.
STRINGi4932.YPR088C.

Structurei

3D structure databases

ProteinModelPortaliP20424.
SMRiP20424.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 303G-domainAdd BLAST303
Regioni304 – 541M-domainAdd BLAST238

Domaini

Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA and also binds the signal sequence.

Sequence similaritiesi

Belongs to the GTP-binding SRP family. SRP54 subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074824.
HOGENOMiHOG000036165.
InParanoidiP20424.
KOiK03106.
OMAiPIKFAGV.
OrthoDBiEOG092C1WCZ.

Family and domain databases

Gene3Di1.10.260.30. 1 hit.
HAMAPiMF_00306. SRP54. 1 hit.
InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR036891. Signal_recog_part_SRP54_M_sf.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR036225. SRP/SRP_N.
IPR022941. SRP54.
IPR006325. SRP54_euk.
IPR000897. SRP54_GTPase_dom.
PANTHERiPTHR11564. PTHR11564. 1 hit.
PfamiView protein in Pfam
PF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
SUPFAMiSSF47364. SSF47364. 1 hit.
SSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01425. SRP54_euk. 1 hit.
PROSITEiView protein in PROSITE
PS00300. SRP54. 1 hit.

Sequencei

Sequence statusi: Complete.

P20424-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLADLGKRI NSAVNNAISN TQDDFTTSVD VMLKGIVTAL LESDVNIALV
60 70 80 90 100
SKLRNNIRSQ LLSENRSEKS TTNAQTKKLI QKTVFDELCK LVTCEGSEEK
110 120 130 140 150
AFVPKKRKTN IIMFVGLQGS GKTTSCTKLA VYYSKRGFKV GLVCADTFRA
160 170 180 190 200
GAFDQLKQNA IRARIPFYGS YTETDPAKVA EEGINKFKKE KFDIIIVDTS
210 220 230 240 250
GRHHQEEELF QEMIEISNVI KPNQTIMVLD ASIGQAAEQQ SKAFKESSDF
260 270 280 290 300
GAIILTKMDG HARGGGAISA VAATNTPIIF IGTGEHIHDL EKFSPKSFIS
310 320 330 340 350
KLLGIGDIES LFEQLQTVSN KEDAKATMEN IQKGKFTLLD FKKQMQTIMK
360 370 380 390 400
MGPLSNIAQM IPGMSNMMNQ VGEEETSQKM KKMVYVLDSM TKEELESDGR
410 420 430 440 450
MFIEEPTRMV RVAKGSGTSV FEVEMILMQQ QMMARMAQTA TQQQPGAPGA
460 470 480 490 500
NARMPGMPNM PGMPNMPGMP NMPGMPKVTP QMMQQAQQKL KQNPGLMQNM
510 520 530 540
MNMFGGGMGG GMGGGMPDMN EMMKMMQDPQ MQQMAKQFGM G
Length:541
Mass (Da):59,624
Last modified:October 1, 1996 - v2
Checksum:i05B1B2C262932F17
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti136R → E in CAA34781 (PubMed:2187859).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16908 Genomic DNA. Translation: CAA34781.1.
M55517 Genomic DNA. Translation: AAA35092.1.
X51614 Genomic DNA. Translation: CAA35952.1.
U51033 Genomic DNA. Translation: AAB68136.1.
BK006949 Genomic DNA. Translation: DAA11505.1.
PIRiS69073. JX0112.
RefSeqiNP_015413.1. NM_001184185.1.

Genome annotation databases

EnsemblFungiiYPR088C; YPR088C; YPR088C.
GeneIDi856203.
KEGGisce:YPR088C.

Similar proteinsi

Entry informationi

Entry nameiSRP54_YEAST
AccessioniPrimary (citable) accession number: P20424
Secondary accession number(s): D6W489
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: October 1, 1996
Last modified: November 22, 2017
This is version 167 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names