P20424 (SRP54_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 116.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Signal recognition particle subunit SRP54 Alternative name(s): Signal recognition particle 54 kDa protein homolog | ||||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | ||||||||
| Taxonomic identifier | 559292 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 541 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Signal-recognition-particle (SRP) assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum (ER) membrane. SRP is required for the cotranslational protein translocation for ER import and preferentially recognizes strongly hydrophobic signal sequences. It is involved in targeting the nascent chain-ribosome (RNC) complex to the ER and is proposed to participate in the arrest of nascent chain elongation during membrane targeting. SRP54 binds to the signal sequence of presecretory protein when they emerge from the ribosomes. SRP54 interacts with the scR1 RNA and mediates the association of the resulting SRP-RNC complex with the signal recognition particle receptor (SR) via its alpha subunit SRP101. Both, SRP54 and SRP101, are locked in their GTP bound forms in the SRP-RNC-SR complex, which dissociates upon transferring the signal sequence to the protein-conducting channel (translocon). After signal sequence transfer, SRP54 and SRP101 act as reciprocal GTPAse-activating proteins (GAPs), thereby resolving their association. Ref.7 Ref.8 |
| Subunit structure | Fungal signal recognition particle (SRP) complex consists of a 7S RNA molecule (scR1) and at least six protein subunits: SRP72, SRP68, SRP54, SEC65, SRP21 and SRP14. SRP54 interacts with SRP101. |
| Subcellular location | |
| Domain | Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA and also binds the signal sequence. |
| Sequence similarities | Belongs to the GTP-binding SRP family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm Signal recognition particle |
| Ligand | GTP-binding Nucleotide-binding RNA-binding |
| Molecular function | GTPase activation Ribonucleoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition Inferred by curator Ref.6. Source: SGD |
| Cellular component | signal recognition particle, endoplasmic reticulum targeting Inferred from direct assay Ref.6. Source: SGD |
| Molecular function | 7S RNA binding Inferred from direct assay. Source: SGD GTP bindingInferred from electronic annotation. Source: UniProtKB-KW GTPase activator activityInferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activityInferred from electronic annotation. Source: InterPro signal sequence bindingInferred from physical interaction Ref.6. Source: SGD |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 541 | 541 | Signal recognition particle subunit SRP54 | PRO_0000101203 | |||||
Regions | |||||||||
| Nucleotide binding | 116 – 123 | 8 | GTP By similarity | ||||||
| Nucleotide binding | 198 – 202 | 5 | GTP By similarity | ||||||
| Nucleotide binding | 256 – 259 | 4 | GTP By similarity | ||||||
| Region | 1 – 303 | 303 | G-domain | ||||||
| Region | 304 – 541 | 238 | M-domain | ||||||
Experimental info | |||||||||
| Sequence conflict | 136 | 1 | R → E in CAA34781. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Isolation of a yeast gene, SRH1, that encodes a homologue of the 54K subunit of mammalian signal recognition particle." Amaya Y., Nakano A., Ito K., Mori M. J. Biochem. 107:457-463(1990) [PubMed: 2187859] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 38626 / AH22 / NRRL Y-12843. |
| [2] | "Saccharomyces cerevisiae and Schizosaccharomyces pombe contain a homologue to the 54-kD subunit of the signal recognition particle that in S. cerevisiae is essential for growth." Hann B.C., Poritz M.A., Walter P. J. Cell Biol. 109:3223-3230(1989) [PubMed: 2557350] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. Hani J.Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [4] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [5] | "Identification of RNA sequences and structural elements required for assembly of fission yeast SRP54 protein with signal recognition particle RNA." Selinger D., Brennwald P., Liao X., Wise J.A. Mol. Cell. Biol. 13:1353-1362(1993) [PubMed: 8382769] [Abstract] Cited for: RNA-BINDING. |
| [6] | "Subunits of the Saccharomyces cerevisiae signal recognition particle required for its functional expression." Brown J.D., Hann B.C., Medzihradszky K.F., Niwa M., Burlingame A.L., Walter P. EMBO J. 13:4390-4400(1994) [PubMed: 7925282] [Abstract] Cited for: IDENTIFICATION IN THE SRP COMPLEX. |
| [7] | "The nascent polypeptide-associated complex modulates interactions between the signal recognition particle and the ribosome." Powers T., Walter P. Curr. Biol. 6:331-338(1996) [PubMed: 8805251] [Abstract] Cited for: FUNCTION. |
| [8] | "Role of Sec61alpha in the regulated transfer of the ribosome-nascent chain complex from the signal recognition particle to the translocation channel." Song W., Raden D., Mandon E.C., Gilmore R. Cell 100:333-343(2000) [PubMed: 10676815] [Abstract] Cited for: FUNCTION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X16908 Genomic DNA. Translation: CAA34781.1. M55517 Genomic DNA. Translation: AAA35092.1. X51614 Genomic DNA. Translation: CAA35952.1. U51033 Genomic DNA. Translation: AAB68136.1. BK006949 Genomic DNA. Translation: DAA11505.1. |
| PIR | JX0112. S69073. |
| RefSeq | NP_015413.1. NM_001184185.1. |
3D structure databases | |
| ProteinModelPortal | P20424. |
| SMR | P20424. Positions 1-445. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-2943N. |
| IntAct | P20424. 24 interactions. |
| MINT | MINT-484768. |
| STRING | P20424. |
Proteomic databases | |
| PeptideAtlas | P20424. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | YPR088C; YPR088C; YPR088C. |
| GeneID | 856203. |
| KEGG | sce:YPR088C. |
| NMPDR | fig|4932.3.peg.6550. |
Organism-specific databases | |
| CYGD | YPR088c. |
| SGD | S000006292. SRP54. |
Phylogenomic databases | |
| eggNOG | fuNOG05098. |
| GeneTree | EFGT00050000005661. |
| HOGENOM | HBG562580. |
| OMA | MGDLAKM. |
| OrthoDB | EOG4TMV9N. |
Gene expression databases | |
| ArrayExpress | P20424. |
| Genevestigator | P20424. |
| GermOnline | YPR088C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR003593. ATPase_AAA+_core. IPR000897. Signal_recog_part_SRP54_GTPase. IPR013822. Signal_recog_particl_SRP54_hlx. IPR006325. Signal_recog_particle_SRP54. IPR004125. Signal_recog_particle_SRP54_M. [Graphical view] |
| Gene3D | G3DSA:1.20.120.140. SRP54_helical. 1 hit. G3DSA:1.10.260.30. SRP54_M. 1 hit. |
| KO | K03106. |
| Pfam | PF00448. SRP54. 1 hit. PF02881. SRP54_N. 1 hit. PF02978. SRP_SPB. 1 hit. [Graphical view] |
| SMART | SM00382. AAA. 1 hit. SM00962. SRP54. 1 hit. SM00963. SRP54_N. 1 hit. [Graphical view] |
| SUPFAM | SSF47446. Signal_recog_particle_SRP54_M. 1 hit. SSF47364. SRP54. 1 hit. |
| TIGRFAMs | TIGR01425. SRP54_euk. 1 hit. |
| PROSITE | PS00300. SRP54. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 981405. |
Entry information
| Entry name | SRP54_YEAST | ||||||||
| Accession | Primary (citable) accession number: P20424 Secondary accession number(s): D6W489 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| SIMILARITY comments Index of protein domains and families |

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