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Protein

Neuronal acetylcholine receptor subunit alpha-5

Gene

Chrna5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

GO - Molecular functioni

  • acetylcholine-activated cation-selective channel activity Source: RGD
  • acetylcholine binding Source: RGD
  • protein heterodimerization activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-5
Gene namesi
Name:Chrna5
Synonyms:Acra5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2347. Chrna5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 239212ExtracellularSequence analysisAdd
BLAST
Transmembranei240 – 26021HelicalSequence analysisAdd
BLAST
Transmembranei269 – 28921HelicalSequence analysisAdd
BLAST
Transmembranei302 – 32221HelicalSequence analysisAdd
BLAST
Topological domaini323 – 41492CytoplasmicSequence analysisAdd
BLAST
Transmembranei415 – 43521HelicalSequence analysisAdd
BLAST
Topological domaini436 – 45217ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

  • acetylcholine-gated channel complex Source: RGD
  • cell junction Source: UniProtKB-KW
  • dendrite Source: RGD
  • neuronal cell body Source: RGD
  • plasma membrane Source: RGD
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2094110.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence analysisAdd
BLAST
Chaini28 – 452425Neuronal acetylcholine receptor subunit alpha-5PRO_0000000358Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi140 – 1401N-linked (GlcNAc...)Sequence analysis
Disulfide bondi155 ↔ 169By similarity
Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence analysis
Glycosylationi214 – 2141N-linked (GlcNAc...)Sequence analysis
Disulfide bondi219 ↔ 220Associated with receptor activationBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP20420.
PRIDEiP20420.

Interactioni

Subunit structurei

Neuronal AChR seems to be composed of two different types of subunits: alpha and non-alpha (beta).

Binary interactionsi

WithEntry#Exp.IntActNotes
Chrna4P094836EBI-10828372,EBI-7842410

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

IntActiP20420. 3 interactions.
STRINGi10116.ENSRNOP00000062227.

Structurei

3D structure databases

ProteinModelPortaliP20420.
SMRiP20420. Positions 235-327.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiP20420.
PhylomeDBiP20420.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 2 hits.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20420-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQLLAGRWR PTGARRGTRG GLPELSSAAK HEDSLFRDLF EDYERWVRPV
60 70 80 90 100
EHLSDKIKIK FGLAISQLVD VDEKNQLMTT NVWLKQEWID VKLRWNPDDY
110 120 130 140 150
GGIKIIRVPS DSLWIPDIVL FDNADGRFEG ASTKTVVRYN GTVTWTQPAN
160 170 180 190 200
YKSSCTIDVT FFPFDLQNCS MKFGSWTYDG SQVDIILEDQ DVDRTDFFDN
210 220 230 240 250
GEWEIMSAMG SKGNRTDSCC WYPYITYSFV IKRLPLFYTL FLIIPCIGLS
260 270 280 290 300
FLTVVVFYLP SNEGEKISLC TSVLVSLTVF LLVIEEIIPS SSKVIPLIGE
310 320 330 340 350
YLVFTMIFVT LSIMVTVFAI NIHHRSSSTH NAMAPWVRKI FLHKLPKLLC
360 370 380 390 400
MRSHADRYFT QREEAESGAG PKSRNTLEAA LDCIRYITRH VVKENDVREV
410 420 430 440 450
VEDWKFIAQV LDRMFLWTFL LVSIIGTLGL FVPVIYKWAN IIVPVHIGNT

IK
Length:452
Mass (Da):51,874
Last modified:February 1, 1991 - v1
Checksum:i7CEB24553936B2E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05231 mRNA. Translation: AAA74475.1.
PIRiA35721.
UniGeneiRn.40125.

Genome annotation databases

UCSCiRGD:2347. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05231 mRNA. Translation: AAA74475.1.
PIRiA35721.
UniGeneiRn.40125.

3D structure databases

ProteinModelPortaliP20420.
SMRiP20420. Positions 235-327.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP20420. 3 interactions.
STRINGi10116.ENSRNOP00000062227.

Chemistry

ChEMBLiCHEMBL2094110.

Proteomic databases

PaxDbiP20420.
PRIDEiP20420.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:2347. rat.

Organism-specific databases

RGDi2347. Chrna5.

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiP20420.
PhylomeDBiP20420.

Miscellaneous databases

PROiP20420.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 2 hits.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Alpha 3, alpha 5, and beta 4: three members of the rat neuronal nicotinic acetylcholine receptor-related gene family form a gene cluster."
    Boulter J., O'Shea-Greenfield A., Duvoisin R.M., Connolly J.G., Wada E., Jensen A., Gardner P.D., Ballivet M., Deneris E.S., McKinnon D., Heinemann S.F., Patrick J.
    J. Biol. Chem. 265:4472-4482(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiACHA5_RAT
AccessioniPrimary (citable) accession number: P20420
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 6, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.