Neuronal acetylcholine receptor subunit alpha-5 precursor

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P20420 (ACHA5_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 107. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Neuronal acetylcholine receptor subunit alpha-5

Gene names

Name:	Chrna5
Synonyms:	Acra5

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Subunit structure	Neuronal AChR seems to be composed of two different types of subunits: alpha and non-alpha (beta).
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-5/CHRNA5 sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Ion channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW dendrite Inferred from direct assay PubMed 11297818. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuronal cell body Inferred from direct assay PubMed 11297818. Source: RGD plasma membrane Inferred from direct assay PubMed 12748066. Source: RGD postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acetylcholine binding Inferred from direct assay PubMed 11297818. Source: RGD acetylcholine-activated cation-selective channel activity Inferred from mutant phenotype PubMed 11297818 PubMed 12814364. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 27

Potential

Chain

28 – 452

425

Neuronal acetylcholine receptor subunit alpha-5

PRO_0000000358

Regions

Topological domain

28 – 239

212

Extracellular Potential

Transmembrane

240 – 260

Helical; Potential

Transmembrane

269 – 289

Helical; Potential

Transmembrane

302 – 322

Helical; Potential

Topological domain

323 – 414

Cytoplasmic Potential

Transmembrane

415 – 435

Helical; Potential

Topological domain

436 – 452

Extracellular Potential

Amino acid modifications

Glycosylation

140

N-linked (GlcNAc...) Potential

Glycosylation

168

N-linked (GlcNAc...) Potential

Glycosylation

214

N-linked (GlcNAc...) Potential

Disulfide bond

155 ↔ 169

By similarity

Disulfide bond

219 ↔ 220

Associated with receptor activation By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P20420 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 7CEB24553936B2E7
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FASTA

452

51,874

        10         20         30         40         50         60 
MVQLLAGRWR PTGARRGTRG GLPELSSAAK HEDSLFRDLF EDYERWVRPV EHLSDKIKIK 

        70         80         90        100        110        120 
FGLAISQLVD VDEKNQLMTT NVWLKQEWID VKLRWNPDDY GGIKIIRVPS DSLWIPDIVL 

       130        140        150        160        170        180 
FDNADGRFEG ASTKTVVRYN GTVTWTQPAN YKSSCTIDVT FFPFDLQNCS MKFGSWTYDG 

       190        200        210        220        230        240 
SQVDIILEDQ DVDRTDFFDN GEWEIMSAMG SKGNRTDSCC WYPYITYSFV IKRLPLFYTL 

       250        260        270        280        290        300 
FLIIPCIGLS FLTVVVFYLP SNEGEKISLC TSVLVSLTVF LLVIEEIIPS SSKVIPLIGE 

       310        320        330        340        350        360 
YLVFTMIFVT LSIMVTVFAI NIHHRSSSTH NAMAPWVRKI FLHKLPKLLC MRSHADRYFT 

       370        380        390        400        410        420 
QREEAESGAG PKSRNTLEAA LDCIRYITRH VVKENDVREV VEDWKFIAQV LDRMFLWTFL 

       430        440        450 
LVSIIGTLGL FVPVIYKWAN IIVPVHIGNT IK

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References

[1]

"Alpha 3, alpha 5, and beta 4: three members of the rat neuronal nicotinic acetylcholine receptor-related gene family form a gene cluster."
Boulter J., O'Shea-Greenfield A., Duvoisin R.M., Connolly J.G., Wada E., Jensen A., Gardner P.D., Ballivet M., Deneris E.S., McKinnon D., Heinemann S.F., Patrick J.
J. Biol. Chem. 265:4472-4482(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J05231 mRNA. Translation: AAA74475.1.
IPI	IPI00325218.
PIR	A35721.
UniGene	Rn.40125.
3D structure databases
ProteinModelPortal	P20420.
SMR	P20420. Positions 235-327.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000062227.
Proteomic databases
PRIDE	P20420.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:2347. rat.
Organism-specific databases
RGD	2347. Chrna5.
Phylogenomic databases
eggNOG	NOG267611.
HOGENOM	HOG000006756.
HOVERGEN	HBG003756.
InParanoid	P20420.
OrthoDB	EOG41JZC6.
Gene expression databases
ArrayExpress	P20420.
Genevestigator	P20420.
GermOnline	ENSRNOG00000013610. Rattus norvegicus.
Family and domain databases
Gene3D	2.70.170.10. 1 hit.
InterPro	IPR006202. Neur_chan_lig-bd. IPR006201. Neur_channel. IPR006029. Neurotrans-gated_channel_TM. IPR018000. Neurotransmitter_ion_chnl_CS. IPR002394. Nicotinic_acetylcholine_rcpt. [Graphical view]
PANTHER	PTHR18945. PTHR18945. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 2 hits. [Graphical view]
PRINTS	PR00254. NICOTINICR. PR00252. NRIONCHANNEL.
SUPFAM	SSF90112. Neu_channel_TM. 1 hit. SSF63712. Neur_chan_LBD. 1 hit.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P20420.
ChEMBL	CHEMBL4960.

Entry information

Entry name

ACHA5_RAT

Accession

Primary (citable) accession number: P20420

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	April 3, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents