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Protein

Tyrosine-protein phosphatase non-receptor type 1

Gene

Ptpn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET (By similarity).By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei181SubstrateBy similarity1
Active sitei215Phosphocysteine intermediatePROSITE-ProRule annotation1
Binding sitei262SubstrateBy similarity1

GO - Molecular functioni

  • cadherin binding Source: Ensembl
  • ephrin receptor binding Source: Ensembl
  • insulin receptor binding Source: RGD
  • protein phosphatase 2A binding Source: RGD
  • protein tyrosine phosphatase activity Source: RGD
  • receptor tyrosine kinase binding Source: Ensembl
  • RNA binding Source: Ensembl
  • zinc ion binding Source: Ensembl

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase

Enzyme and pathway databases

ReactomeiR-RNO-354192. Integrin alphaIIb beta3 signaling.
R-RNO-6807004. Negative regulation of MET activity.
R-RNO-8849472. PTK6 Down-Regulation.
R-RNO-912694. Regulation of IFNA signaling.
R-RNO-982772. Growth hormone receptor signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein phosphatase non-receptor type 1 (EC:3.1.3.48)
Alternative name(s):
Protein-tyrosine phosphatase 1B
Short name:
PTP-1B
Gene namesi
Name:Ptpn1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi61965. Ptpn1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2371.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000947501 – 432Tyrosine-protein phosphatase non-receptor type 1Add BLAST432

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei20PhosphotyrosineBy similarity1
Modified residuei50Phosphoserine; by PKB/AKT1, CLK1 and CLK2By similarity1
Modified residuei66Phosphotyrosine; by EGFRBy similarity1
Modified residuei215Cysteine persulfideBy similarity1
Modified residuei215S-nitrosocysteine; in reversibly inhibited formBy similarity1
Modified residuei242Phosphoserine; by CLK1 and CLK2By similarity1
Modified residuei243Phosphoserine; by CLK1 and CLK2By similarity1
Modified residuei335PhosphoserineBy similarity1
Modified residuei362PhosphoserineBy similarity1
Modified residuei364PhosphoserineBy similarity1
Modified residuei367PhosphothreonineBy similarity1

Post-translational modificationi

Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50 (By similarity).By similarity
S-nitrosylation of Cys-215 inactivates the enzyme activity.By similarity
Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, S-nitrosylation

Proteomic databases

PaxDbiP20417.
PRIDEiP20417.

PTM databases

iPTMnetiP20417.
PhosphoSitePlusiP20417.

Expressioni

Tissue specificityi

Found in several tissues including central nervous system, liver and kidney. A high level of expression was found in the hippocampus.

Gene expression databases

BgeeiENSRNOG00000010574.

Interactioni

Subunit structurei

Interacts with EPHA3 (phosphorylated); dephosphorylates EPHA3 and may regulate its trafficking and function. Interacts with MET.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
EGFRP0053311EBI-916819,EBI-297353From Homo sapiens.

GO - Molecular functioni

  • cadherin binding Source: Ensembl
  • ephrin receptor binding Source: Ensembl
  • insulin receptor binding Source: RGD
  • protein phosphatase 2A binding Source: RGD
  • receptor tyrosine kinase binding Source: Ensembl

Protein-protein interaction databases

CORUMiP20417.
IntActiP20417. 3 interactors.
STRINGi10116.ENSRNOP00000014309.

Chemistry databases

BindingDBiP20417.

Structurei

3D structure databases

ProteinModelPortaliP20417.
SMRiP20417.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 277Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST275

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni215 – 221Substrate bindingBy similarity7

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0789. Eukaryota.
COG5599. LUCA.
GeneTreeiENSGT00900000140785.
HOGENOMiHOG000273908.
HOVERGENiHBG008321.
InParanoidiP20417.
KOiK05696.
OMAiGSIKCAQ.
OrthoDBiEOG091G082B.
PhylomeDBiP20417.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiView protein in InterPro
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR012265. Ptpn1/Ptpn2.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
PfamiView protein in Pfam
PF00102. Y_phosphatase. 1 hit.
PIRSFiPIRSF000926. Tyr-Ptase_nr1. 1 hit.
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiView protein in SMART
SM00194. PTPc. 1 hit.
SM00404. PTPc_motif. 1 hit.
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiView protein in PROSITE
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.

Sequencei

Sequence statusi: Complete.

P20417-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMEKEFEQI DKAGNWAAIY QDIRHEASDF PCRIAKLPKN KNRNRYRDVS
60 70 80 90 100
PFDHSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW
110 120 130 140 150
EQKSRGVVML NRIMEKGSLK CAQYWPQKEE KEMVFDDTNL KLTLISEDVK
160 170 180 190 200
SYYTVRQLEL ENLATQEARE ILHFHYTTWP DFGVPESPAS FLNFLFKVRE
210 220 230 240 250
SGSLSPEHGP IVVHCSAGIG RSGTFCLADT CLLLMDKRKD PSSVDIKKVL
260 270 280 290 300
LEMRRFRMGL IQTADQLRFS YLAVIEGAKF IMGDSSVQDQ WKELSHEDLE
310 320 330 340 350
PPPEHVPPPP RPPKRTLEPH NGKCKELFSN HQWVSEESCE DEDILAREES
360 370 380 390 400
RAPSIAVHSM SSMSQDTEVR KRMVGGGLQS AQASVPTEEE LSPTEEEQKA
410 420 430
HRPVHWKPFL VNVCMATALA TGAYLCYRVC FH
Length:432
Mass (Da):49,674
Last modified:February 1, 1991 - v1
Checksum:i0D5859A09B1885C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33962 mRNA. Translation: AAC79516.1.
BC081829 mRNA. Translation: AAH81829.1.
PIRiA34845.
RefSeqiNP_036769.1. NM_012637.2.
UniGeneiRn.11317.

Genome annotation databases

EnsembliENSRNOT00000014309; ENSRNOP00000014309; ENSRNOG00000010574.
GeneIDi24697.
KEGGirno:24697.
UCSCiRGD:61965. rat.

Similar proteinsi

Entry informationi

Entry nameiPTN1_RAT
AccessioniPrimary (citable) accession number: P20417
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: October 25, 2017
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families