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Protein

Tyrosine-protein phosphatase non-receptor type 1

Gene

Ptpn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET (By similarity).By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei181 – 1811SubstrateBy similarity
Active sitei215 – 2151Phosphocysteine intermediatePROSITE-ProRule annotation
Binding sitei262 – 2621SubstrateBy similarity

GO - Molecular functioni

  • insulin receptor binding Source: RGD
  • poly(A) RNA binding Source: Ensembl
  • protein phosphatase 2A binding Source: RGD
  • protein tyrosine phosphatase activity Source: RGD
  • zinc ion binding Source: Ensembl

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Enzyme and pathway databases

ReactomeiREACT_275527. Regulation of IFNA signaling.
REACT_309732. Regulation of IFNG signaling.
REACT_314415. Growth hormone receptor signaling.
REACT_314992. Integrin alphaIIb beta3 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein phosphatase non-receptor type 1 (EC:3.1.3.48)
Alternative name(s):
Protein-tyrosine phosphatase 1B
Short name:
PTP-1B
Gene namesi
Name:Ptpn1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi61965. Ptpn1.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: RGD
  • cytoplasmic side of endoplasmic reticulum membrane Source: Ensembl
  • cytoplasmic vesicle Source: RGD
  • cytosol Source: RGD
  • early endosome Source: UniProtKB
  • plasma membrane Source: Ensembl
  • sorting endosome Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 432432Tyrosine-protein phosphatase non-receptor type 1PRO_0000094750Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei20 – 201PhosphotyrosineBy similarity
Modified residuei50 – 501Phosphoserine; by PKB/AKT1, CLK1 and CLK2By similarity
Modified residuei66 – 661Phosphotyrosine; by EGFRBy similarity
Modified residuei215 – 2151Cysteine persulfideBy similarity
Modified residuei215 – 2151S-nitrosocysteine; in reversibly inhibited formBy similarity
Modified residuei242 – 2421Phosphoserine; by CLK1 and CLK2By similarity
Modified residuei243 – 2431Phosphoserine; by CLK1 and CLK2By similarity

Post-translational modificationi

Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50 (By similarity).By similarity
S-nitrosylation of Cys-215 inactivates the enzyme activity.By similarity
Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, S-nitrosylation

Proteomic databases

PaxDbiP20417.
PRIDEiP20417.

Expressioni

Tissue specificityi

Found in several tissues including central nervous system, liver and kidney. A high level of expression was found in the hippocampus.

Gene expression databases

GenevisibleiP20417. RN.

Interactioni

Subunit structurei

Interacts with EPHA3 (phosphorylated); dephosphorylates EPHA3 and may regulate its trafficking and function. Interacts with MET.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
EGFRP0053311EBI-916819,EBI-297353From a different organism.

Protein-protein interaction databases

IntActiP20417. 3 interactions.
STRINGi10116.ENSRNOP00000014309.

Structurei

3D structure databases

ProteinModelPortaliP20417.
SMRiP20417. Positions 1-299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 277275Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni215 – 2217Substrate bindingBy similarity

Sequence similaritiesi

Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5599.
GeneTreeiENSGT00770000120452.
HOGENOMiHOG000273908.
HOVERGENiHBG008321.
InParanoidiP20417.
KOiK05696.
OMAiDGPIKEE.
OrthoDBiEOG7RV9G4.
PhylomeDBiP20417.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR012265. Ptpn1/Ptpn2.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF000926. Tyr-Ptase_nr1. 1 hit.
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20417-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMEKEFEQI DKAGNWAAIY QDIRHEASDF PCRIAKLPKN KNRNRYRDVS
60 70 80 90 100
PFDHSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW
110 120 130 140 150
EQKSRGVVML NRIMEKGSLK CAQYWPQKEE KEMVFDDTNL KLTLISEDVK
160 170 180 190 200
SYYTVRQLEL ENLATQEARE ILHFHYTTWP DFGVPESPAS FLNFLFKVRE
210 220 230 240 250
SGSLSPEHGP IVVHCSAGIG RSGTFCLADT CLLLMDKRKD PSSVDIKKVL
260 270 280 290 300
LEMRRFRMGL IQTADQLRFS YLAVIEGAKF IMGDSSVQDQ WKELSHEDLE
310 320 330 340 350
PPPEHVPPPP RPPKRTLEPH NGKCKELFSN HQWVSEESCE DEDILAREES
360 370 380 390 400
RAPSIAVHSM SSMSQDTEVR KRMVGGGLQS AQASVPTEEE LSPTEEEQKA
410 420 430
HRPVHWKPFL VNVCMATALA TGAYLCYRVC FH
Length:432
Mass (Da):49,674
Last modified:February 1, 1991 - v1
Checksum:i0D5859A09B1885C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33962 mRNA. Translation: AAC79516.1.
BC081829 mRNA. Translation: AAH81829.1.
PIRiA34845.
RefSeqiNP_036769.1. NM_012637.2.
XP_006235700.1. XM_006235638.2.
UniGeneiRn.11317.

Genome annotation databases

EnsembliENSRNOT00000014309; ENSRNOP00000014309; ENSRNOG00000010574.
GeneIDi24697.
KEGGirno:24697.
UCSCiRGD:61965. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33962 mRNA. Translation: AAC79516.1.
BC081829 mRNA. Translation: AAH81829.1.
PIRiA34845.
RefSeqiNP_036769.1. NM_012637.2.
XP_006235700.1. XM_006235638.2.
UniGeneiRn.11317.

3D structure databases

ProteinModelPortaliP20417.
SMRiP20417. Positions 1-299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP20417. 3 interactions.
STRINGi10116.ENSRNOP00000014309.

Chemistry

BindingDBiP20417.
ChEMBLiCHEMBL2371.

Proteomic databases

PaxDbiP20417.
PRIDEiP20417.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000014309; ENSRNOP00000014309; ENSRNOG00000010574.
GeneIDi24697.
KEGGirno:24697.
UCSCiRGD:61965. rat.

Organism-specific databases

CTDi5770.
RGDi61965. Ptpn1.

Phylogenomic databases

eggNOGiCOG5599.
GeneTreeiENSGT00770000120452.
HOGENOMiHOG000273908.
HOVERGENiHBG008321.
InParanoidiP20417.
KOiK05696.
OMAiDGPIKEE.
OrthoDBiEOG7RV9G4.
PhylomeDBiP20417.

Enzyme and pathway databases

ReactomeiREACT_275527. Regulation of IFNA signaling.
REACT_309732. Regulation of IFNG signaling.
REACT_314415. Growth hormone receptor signaling.
REACT_314992. Integrin alphaIIb beta3 signaling.

Miscellaneous databases

NextBioi604151.
PROiP20417.

Gene expression databases

GenevisibleiP20417. RN.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR012265. Ptpn1/Ptpn2.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF000926. Tyr-Ptase_nr1. 1 hit.
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  3. "Insulin receptor and epidermal growth factor receptor dephosphorylation by three major rat liver protein-tyrosine phosphatases expressed in a recombinant bacterial system."
    Hashimoto N., Zhang W.R., Goldstein B.J.
    Biochem. J. 284:569-576(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 35-283.

Entry informationi

Entry nameiPTN1_RAT
AccessioniPrimary (citable) accession number: P20417
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 22, 2015
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.