P20414 (TIMP1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metalloproteinase inhibitor 1

Alternative name(s):
Embryogenin-1
Short name=EG-1
Tissue inhibitor of metalloproteinases 1
Short name=TIMP-1

Gene names

Name:

TIMP1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
Subcellular location	Secreted.
Post-translational modification	The activity of TIMP1 is dependent on the presence of disulfide bonds.
Sequence similarities	Belongs to the protease inhibitor I35 (TIMP) family. [View classification] Contains 1 NTR domain.

Ontologies

Keywords
Biological process	Erythrocyte maturation
Cellular component	Secreted
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Metalloenzyme inhibitor Metalloprotease inhibitor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	erythrocyte maturation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.6
	Chain	24 – 207	184	Metalloproteinase inhibitor 1		PRO_0000034320
Regions
	Domain	24 – 147	124	NTR
	Region	24 – 28	5	Involved in metalloproteinase-binding By similarity
	Region	90 – 91	2	Involved in metalloproteinase-binding By similarity
Sites
	Metal binding	24	1	Zinc; via amino nitrogen and carbonyl oxygen; shared with metalloproteinase partner By similarity
Amino acid modifications
	Glycosylation	53	1	N-linked (GlcNAc...) Potential
	Glycosylation	101	1	N-linked (GlcNAc...) Potential
	Disulfide bond	24 ↔ 93		By similarity
	Disulfide bond	26 ↔ 122		By similarity
	Disulfide bond	36 ↔ 147		By similarity
	Disulfide bond	150 ↔ 197		By similarity
	Disulfide bond	155 ↔ 160		By similarity
	Disulfide bond	168 ↔ 189		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P20414 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: E672BEE2E865F3F7	FASTA	207	23,032
	10 20 30 40 50 60 MAPFAPMASG ILLLLWLTAP SRACTCVPPH PQTAFCNSDV VIRAKFVGTA EVNETALYQR 70 80 90 100 110 120 YEIKMTKMFK GFSALRDAPD IRFIYTPAME SVCGYFHRSQ NRSEEFLIAG QLSNGHLHIT 130 140 150 160 170 180 TCSFVAPWNS MSSAQRRGFT KTYAAGCEEC TVFPCSSIPC KLQSDTHCLW TDQLLTGSDK 190 200 GFQSRHLACL PREPGLCTWQ SLRAQMA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"mRNA of bovine tissue inhibitor of metalloproteinase: sequence and expression in bovine ovarian tissue." Freudenstein J., Wagner S., Luck R.M., Einspanier R., Scheit K.H. Biochem. Biophys. Res. Commun. 171:250-256(1990) [PubMed: 2393392] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Tissue inhibitor of metalloproteinases (TIMP-1) produced by granulosa and oviduct cells enhances in vitro development of bovine embryo." Satoh T., Kobayashi K., Yamashita S., Kikuchi M., Sendai Y., Hoshi H. Biol. Reprod. 50:835-844(1994) [PubMed: 8199264] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"ACTH induces TIMP-1 expression and inhibits collagenase in adrenal cortex cells." Reichenstein M., Reich R., Lehoux J.-G., Hanukoglu I. Mol. Cell. Endocrinol. 215:109-114(2004) [PubMed: 15026182] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adrenal cortex.
[4]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.
[5]	"Involvement of fibroblasts and muscle cells in the expression of an extracellular proteolytic cascade in bovine skeletal muscle." Balcerzak D., Querengesser L., Dixon W.T., Baracos V.E. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-191. Tissue: Skeletal muscle.
[6]	"Purification and characterization of two related but distinct metalloproteinase inhibitors secreted by bovine aortic endothelial cells." de Clerck Y.A., Yean T.D., Ratzkin B.J., Lu H.S., Langley K.E. J. Biol. Chem. 264:17445-17453(1989) [PubMed: 2551903] [Abstract] Cited for: PRELIMINARY PROTEIN SEQUENCE OF 24-69.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M60073 mRNA. Translation: AAA30784.1. S70841 mRNA. Translation: AAB30892.1. AY295346 mRNA. Translation: AAP44413.1. BC102489 mRNA. Translation: AAI02490.1. AF144763 mRNA. Translation: AAD30303.1.
IPI	IPI00709084.
PIR	A35685.
RefSeq	NP_776896.1. NM_174471.3.
UniGene	Bt.27976.
3D structure databases
ProteinModelPortal	P20414.
SMR	P20414. Positions 24-204.
ModBase	Search...
Protein-protein interaction databases
STRING	P20414.
Proteomic databases
PRIDE	P20414.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000006653; ENSBTAP00000006653; ENSBTAG00000005043.
GeneID	282092.
KEGG	bta:282092.
Organism-specific databases
CTD	7076.
Phylogenomic databases
eggNOG	maNOG18103.
HOVERGEN	HBG068749.
InParanoid	P20414.
OMA	SSIPCKL.
OrthoDB	EOG44J2K2.
PhylomeDB	P20414.
Family and domain databases
InterPro	IPR001134. Netrin_domain. IPR001820. Prot_inh_TIMP. IPR008993. TIMP-like_OB-fold. IPR015611. TIMP1. [Graphical view]
PANTHER	PTHR11844. Prot_inh_TIMP. 1 hit. PTHR11844:SF5. TIMP1. 1 hit.
Pfam	PF00965. TIMP. 1 hit. [Graphical view]
SMART	SM00206. NTR. 1 hit. [Graphical view]
SUPFAM	SSF50242. TIMP_like. 1 hit.
PROSITE	PS50189. NTR. 1 hit. PS00288. TIMP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TIMP1_BOVIN

Accession

Primary (citable) accession number: P20414
Secondary accession number(s): Q3T098, Q53ZP2, Q9TVB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	October 19, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program