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P20409 (TRPG_PHYBL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Multifunctional tryptophan biosynthesis protein

Including the following 3 domains:

  1. Anthranilate synthase component 2
    Short name=AS
    EC=4.1.3.27
    Alternative name(s):
    Anthranilate synthase, glutamine amidotransferase component
  2. Indole-3-glycerol phosphate synthase
    Short name=IGPS
    EC=4.1.1.48
  3. N-(5'-phosphoribosyl)anthranilate isomerase
    Short name=PRAI
    EC=5.3.1.24
Gene names
Name:trp1
OrganismPhycomyces blakesleeanus
Taxonomic identifier4837 [NCBI]
Taxonomic lineageEukaryotaFungiFungi incertae sedisEarly diverging fungal lineagesMucoromycotinaMucoralesPhycomycetaceaePhycomyces

Protein attributes

Sequence length765 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities. HAMAP-Rule MF_00135

Catalytic activity

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate. HAMAP-Rule MF_00135

1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O. HAMAP-Rule MF_00135

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate. HAMAP-Rule MF_00135

Pathway

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5. HAMAP-Rule MF_00135

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.

Sequence similarities

Contains 1 glutamine amidotransferase type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 765765Multifunctional tryptophan biosynthesis protein HAMAP-Rule MF_00135
PRO_0000056864

Regions

Domain2 – 196195Glutamine amidotransferase type-1
Region231 – 494264Indole-3-glycerol phosphate synthase HAMAP-Rule MF_00135
Region512 – 765254N-(5'-phosphoribosyl)anthranilate isomerase HAMAP-Rule MF_00135

Sites

Active site811For GATase activity By similarity
Active site1701For GATase activity By similarity
Active site1721For GATase activity By similarity

Experimental info

Sequence conflict6061I → T in AAA33633. Ref.2
Sequence conflict6171G → A in AAA33633. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P20409 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: BDE6D904C065F58C

FASTA76583,423
        10         20         30         40         50         60 
MATLLIDNYD SFTYNVYQYL CSQGADVVVY RNDKITVDEI VKLNPVNIVI SPGPGHPSHD 

        70         80         90        100        110        120 
AGVSRDVISY FAGKLPILGI CMGEQCIFEV FGGTVSYAGD ILHGKTSTIK HDNRGLFKNV 

       130        140        150        160        170        180 
PQDNQVTRYH SLAGMPSTLP EVLEVTATTD DGVIMGVRHK KYTVEGVQFH PESILCEHGH 

       190        200        210        220        230        240 
TMISNFLSLR GGNWDENPAA GVLAQKVPAA ATEKAAQEAS PAISTPPTCS TILSRIYAQR 

       250        260        270        280        290        300 
VKDVQAAKEV PGQSQADLQK LLNLHIAPPL RDVVNRLKES SPALMAEVKR ASPSKGNIDI 

       310        320        330        340        350        360 
TVNAAEQALQ YALAGASVIS VLTEPKWFRG SLNDLRQVRE ACPLLPNRPC ILRKTFLLDT 

       370        380        390        400        410        420 
YQILEARLYG ADTVLLIVAM MSDEDLRELY QYSVSLGMEP LVEVNNAEEM ARANAVGAKL 

       430        440        450        460        470        480 
IGVNNRGLHS FDVDMETTSR LAEMVPEGTI LCALSGISTR ADVETYVSQG VHGLLVGEAL 

       490        500        510        520        530        540 
MRAWNLKEFV AELLGYKKKD PVPHTPVSRQ VQVKICGISS VEAAVEAATA GADLVGLIFA 

       550        560        570        580        590        600 
EKSKRQVTVA KAREIVDALH KLPTRSSQLP VKSQKSIDWF DVQTEMVEQR VPWRPLVVGV 

       610        620        630        640        650        660 
FVNQSIEYMS QVAVEAGLDL IQLHGTESAE IARFLPVPVI KAFHMDASSF HAGQIPYVTQ 

       670        680        690        700        710        720 
PGNNQLLLLD AKVPSLPMDR QGGLGQKFDW TIAQDIVNVK RPGCSKEQTF PVILAGGLDP 

       730        740        750        760 
SNISEAIQQV RPWAVDVSSG VETDGKKDLK KIRAFVEKAK SINLQ 

« Hide

References

[1]"Phycomyces blakesleeanus TRP1 gene: organization and functional complementation in Escherichia coli and Saccharomyces cerevisiae."
Revuelta J.L., Jayaram M.
Mol. Cell. Biol. 7:2664-2670(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Structural organization of the TRP1 gene of Phycomyces blakesleeanus: implications for evolutionary gene fusion in fungi."
Choi H.T., Revuelta J.L., Sadhu C., Jayaram M.
Gene 71:85-95(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M17240 Genomic DNA. No translation available.
M17241 Genomic DNA. No translation available.
M23177 Genomic DNA. Translation: AAA33633.1.
PIRJT0383.

3D structure databases

ProteinModelPortalP20409.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00035; UER00040.
UPA00035; UER00042.
UPA00035; UER00043.

Family and domain databases

Gene3D3.20.20.70. 3 hits.
HAMAPMF_00135. PRAI.
InterProIPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI_dom.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PANTHERPTHR11922:SF3. PTHR11922:SF3. 1 hit.
PfamPF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view]
PIRSFPIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMSSF51366. SSF51366. 3 hits.
TIGRFAMsTIGR00566. trpG_papA. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRPG_PHYBL
AccessionPrimary (citable) accession number: P20409
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: February 19, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways