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Protein

Multifunctional tryptophan biosynthesis protein

Gene

trp1

Organism
Phycomyces blakesleeanus
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.

Catalytic activityi

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.
Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1, 3 and 4 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Multifunctional tryptophan biosynthesis protein (trp1)
  2. no protein annotated in this organism
  3. Multifunctional tryptophan biosynthesis protein (trp1)
  4. Multifunctional tryptophan biosynthesis protein (trp1)
  5. no protein annotated in this organism
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei81Nucleophile; for GATase activityBy similarity1
Binding sitei85GlutamineBy similarity1
Active sitei170For GATase activityBy similarity1
Active sitei172For GATase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Isomerase, Lyase, Multifunctional enzyme
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040
UPA00035; UER00042
UPA00035; UER00043

Protein family/group databases

MEROPSiC26.959

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional tryptophan biosynthesis protein
Including the following 3 domains:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Alternative name(s):
Anthranilate synthase, glutamine amidotransferase component
Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
Short name:
IGPS
N-(5'-phosphoribosyl)anthranilate isomerase (EC:5.3.1.24)
Short name:
PRAI
Gene namesi
Name:trp1
OrganismiPhycomyces blakesleeanus
Taxonomic identifieri4837 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisMucoromycotaMucoromycotinaMucoralesPhycomycetaceaePhycomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000568641 – 765Multifunctional tryptophan biosynthesis proteinAdd BLAST765

Proteomic databases

PRIDEiP20409

Structurei

3D structure databases

ProteinModelPortaliP20409
SMRiP20409
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 196Glutamine amidotransferase type-1Add BLAST195

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni53 – 55Glutamine bindingBy similarity3
Regioni131 – 132Glutamine bindingBy similarity2
Regioni231 – 494Indole-3-glycerol phosphate synthaseAdd BLAST264
Regioni512 – 765N-(5'-phosphoribosyl)anthranilate isomeraseAdd BLAST254

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

CDDicd00331 IGPS, 1 hit
cd00405 PRAI, 1 hit
Gene3Di3.20.20.70, 1 hit
3.40.50.880, 1 hit
HAMAPiMF_00135 PRAI, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR016302 Anthranilate_synth_II
IPR029062 Class_I_gatase-like
IPR017926 GATASE
IPR013798 Indole-3-glycerol_P_synth
IPR001468 Indole-3-GlycerolPSynthase_CS
IPR001240 PRAI
IPR011060 RibuloseP-bd_barrel
IPR006221 TrpG/PapA_dom
PfamiView protein in Pfam
PF00117 GATase, 1 hit
PF00218 IGPS, 1 hit
PF00697 PRAI, 2 hits
PIRSFiPIRSF001382 TrpG-trpC-trpF, 1 hit
SUPFAMiSSF51366 SSF51366, 3 hits
SSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR00566 trpG_papA, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit
PS00614 IGPS, 1 hit

Sequencei

Sequence statusi: Complete.

P20409-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLLIDNYD SFTYNVYQYL CSQGADVVVY RNDKITVDEI VKLNPVNIVI
60 70 80 90 100
SPGPGHPSHD AGVSRDVISY FAGKLPILGI CMGEQCIFEV FGGTVSYAGD
110 120 130 140 150
ILHGKTSTIK HDNRGLFKNV PQDNQVTRYH SLAGMPSTLP EVLEVTATTD
160 170 180 190 200
DGVIMGVRHK KYTVEGVQFH PESILCEHGH TMISNFLSLR GGNWDENPAA
210 220 230 240 250
GVLAQKVPAA ATEKAAQEAS PAISTPPTCS TILSRIYAQR VKDVQAAKEV
260 270 280 290 300
PGQSQADLQK LLNLHIAPPL RDVVNRLKES SPALMAEVKR ASPSKGNIDI
310 320 330 340 350
TVNAAEQALQ YALAGASVIS VLTEPKWFRG SLNDLRQVRE ACPLLPNRPC
360 370 380 390 400
ILRKTFLLDT YQILEARLYG ADTVLLIVAM MSDEDLRELY QYSVSLGMEP
410 420 430 440 450
LVEVNNAEEM ARANAVGAKL IGVNNRGLHS FDVDMETTSR LAEMVPEGTI
460 470 480 490 500
LCALSGISTR ADVETYVSQG VHGLLVGEAL MRAWNLKEFV AELLGYKKKD
510 520 530 540 550
PVPHTPVSRQ VQVKICGISS VEAAVEAATA GADLVGLIFA EKSKRQVTVA
560 570 580 590 600
KAREIVDALH KLPTRSSQLP VKSQKSIDWF DVQTEMVEQR VPWRPLVVGV
610 620 630 640 650
FVNQSIEYMS QVAVEAGLDL IQLHGTESAE IARFLPVPVI KAFHMDASSF
660 670 680 690 700
HAGQIPYVTQ PGNNQLLLLD AKVPSLPMDR QGGLGQKFDW TIAQDIVNVK
710 720 730 740 750
RPGCSKEQTF PVILAGGLDP SNISEAIQQV RPWAVDVSSG VETDGKKDLK
760
KIRAFVEKAK SINLQ
Length:765
Mass (Da):83,423
Last modified:February 1, 1991 - v1
Checksum:iBDE6D904C065F58C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti606I → T in AAA33633 (PubMed:3215529).Curated1
Sequence conflicti617G → A in AAA33633 (PubMed:3215529).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17240 Genomic DNA No translation available.
M17241 Genomic DNA No translation available.
M23177 Genomic DNA Translation: AAA33633.1
PIRiJT0383

Similar proteinsi

Entry informationi

Entry nameiTRPG_PHYBL
AccessioniPrimary (citable) accession number: P20409
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: May 23, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

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