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Protein

Multifunctional tryptophan biosynthesis protein

Gene

trp1

Organism
Phycomyces blakesleeanus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.

Catalytic activityi

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.
Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei81 – 811For GATase activityBy similarity
Active sitei170 – 1701For GATase activityBy similarity
Active sitei172 – 1721For GATase activityBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Isomerase, Lyase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
UPA00035; UER00042.
UPA00035; UER00043.

Protein family/group databases

MEROPSiC26.959.

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional tryptophan biosynthesis protein
Including the following 3 domains:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Alternative name(s):
Anthranilate synthase, glutamine amidotransferase component
Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
Short name:
IGPS
N-(5'-phosphoribosyl)anthranilate isomerase (EC:5.3.1.24)
Short name:
PRAI
Gene namesi
Name:trp1
OrganismiPhycomyces blakesleeanus
Taxonomic identifieri4837 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisEarly diverging fungal lineagesMucoromycotinaMucoralesPhycomycetaceaePhycomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 765765Multifunctional tryptophan biosynthesis proteinPRO_0000056864Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP20409.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 196195Glutamine amidotransferase type-1Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni231 – 494264Indole-3-glycerol phosphate synthaseAdd
BLAST
Regioni512 – 765254N-(5'-phosphoribosyl)anthranilate isomeraseAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

Gene3Di3.20.20.70. 3 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00135. PRAI.
InterProiIPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view]
PIRSFiPIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMiSSF51366. SSF51366. 3 hits.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20409-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLLIDNYD SFTYNVYQYL CSQGADVVVY RNDKITVDEI VKLNPVNIVI
60 70 80 90 100
SPGPGHPSHD AGVSRDVISY FAGKLPILGI CMGEQCIFEV FGGTVSYAGD
110 120 130 140 150
ILHGKTSTIK HDNRGLFKNV PQDNQVTRYH SLAGMPSTLP EVLEVTATTD
160 170 180 190 200
DGVIMGVRHK KYTVEGVQFH PESILCEHGH TMISNFLSLR GGNWDENPAA
210 220 230 240 250
GVLAQKVPAA ATEKAAQEAS PAISTPPTCS TILSRIYAQR VKDVQAAKEV
260 270 280 290 300
PGQSQADLQK LLNLHIAPPL RDVVNRLKES SPALMAEVKR ASPSKGNIDI
310 320 330 340 350
TVNAAEQALQ YALAGASVIS VLTEPKWFRG SLNDLRQVRE ACPLLPNRPC
360 370 380 390 400
ILRKTFLLDT YQILEARLYG ADTVLLIVAM MSDEDLRELY QYSVSLGMEP
410 420 430 440 450
LVEVNNAEEM ARANAVGAKL IGVNNRGLHS FDVDMETTSR LAEMVPEGTI
460 470 480 490 500
LCALSGISTR ADVETYVSQG VHGLLVGEAL MRAWNLKEFV AELLGYKKKD
510 520 530 540 550
PVPHTPVSRQ VQVKICGISS VEAAVEAATA GADLVGLIFA EKSKRQVTVA
560 570 580 590 600
KAREIVDALH KLPTRSSQLP VKSQKSIDWF DVQTEMVEQR VPWRPLVVGV
610 620 630 640 650
FVNQSIEYMS QVAVEAGLDL IQLHGTESAE IARFLPVPVI KAFHMDASSF
660 670 680 690 700
HAGQIPYVTQ PGNNQLLLLD AKVPSLPMDR QGGLGQKFDW TIAQDIVNVK
710 720 730 740 750
RPGCSKEQTF PVILAGGLDP SNISEAIQQV RPWAVDVSSG VETDGKKDLK
760
KIRAFVEKAK SINLQ
Length:765
Mass (Da):83,423
Last modified:February 1, 1991 - v1
Checksum:iBDE6D904C065F58C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti606 – 6061I → T in AAA33633 (PubMed:3215529).Curated
Sequence conflicti617 – 6171G → A in AAA33633 (PubMed:3215529).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17240 Genomic DNA. No translation available.
M17241 Genomic DNA. No translation available.
M23177 Genomic DNA. Translation: AAA33633.1.
PIRiJT0383.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17240 Genomic DNA. No translation available.
M17241 Genomic DNA. No translation available.
M23177 Genomic DNA. Translation: AAA33633.1.
PIRiJT0383.

3D structure databases

ProteinModelPortaliP20409.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC26.959.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
UPA00035; UER00042.
UPA00035; UER00043.

Family and domain databases

Gene3Di3.20.20.70. 3 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00135. PRAI.
InterProiIPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view]
PIRSFiPIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMiSSF51366. SSF51366. 3 hits.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Phycomyces blakesleeanus TRP1 gene: organization and functional complementation in Escherichia coli and Saccharomyces cerevisiae."
    Revuelta J.L., Jayaram M.
    Mol. Cell. Biol. 7:2664-2670(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Structural organization of the TRP1 gene of Phycomyces blakesleeanus: implications for evolutionary gene fusion in fungi."
    Choi H.T., Revuelta J.L., Sadhu C., Jayaram M.
    Gene 71:85-95(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiTRPG_PHYBL
AccessioniPrimary (citable) accession number: P20409
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: April 1, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.