ID   ROB1_RHIRH              Reviewed;         259 AA.
AC   P20402;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   24-JAN-2024, entry version 57.
DE   RecName: Full=Protein-tyrosine phosphatase RolB;
DE            Short=ROL B protein;
DE            EC=3.1.3.48;
GN   Name=rolB;
OS   Rhizobium rhizogenes (Agrobacterium rhizogenes).
OG   Plasmid pRiA4b.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=359;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=A4;
RA   Furner I.J., Huffman G.A., Amasino R.M., Garfinkel D.J., Gordon M.P.,
RA   Nester E.W.;
RT   "An Agrobacterium transformation in the evolution of the genus Nicotiana.";
RL   Nature 319:422-427(1986).
RN   [2]
RP   PRELIMINARY FUNCTION.
RX   PubMed=1915286; DOI=10.1002/j.1460-2075.1991.tb04873.x;
RA   Estruch J.J., Schell J., Spena A.;
RT   "The protein encoded by the rolB plant oncogene hydrolyses indole
RT   glucosides.";
RL   EMBO J. 10:3125-3128(1991).
RN   [3]
RP   FUNCTION.
RX   PubMed=8596628; DOI=10.1038/379499a0;
RA   Filippini F., Rossi V., Marin O., Trovato M., Constantino P., Downey P.M.,
RA   Lo Schiavo F., Terzi M.;
RT   "A plant oncogene as a phosphatase.";
RL   Nature 379:499-500(1996).
CC   -!- FUNCTION: Induces differentiation and growth of neoplastic roots (hairy
CC       roots). Seems to function as a tyrosine phosphatase.
CC       {ECO:0000269|PubMed:8596628}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] +
CC         phosphate; Xref=Rhea:RHEA:10684, Rhea:RHEA-COMP:10136, Rhea:RHEA-
CC         COMP:10137, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:46858,
CC         ChEBI:CHEBI:82620; EC=3.1.3.48;
CC   -!- CAUTION: Was originally thought to release indoles from indoxyl-beta-
CC       glucosides. {ECO:0000305|PubMed:1915286}.
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DR   EMBL; X03433; CAA27163.1; -; Genomic_DNA.
DR   PIR; A27259; A27259.
DR   AlphaFoldDB; P20402; -.
DR   GO; GO:0004725; F:protein tyrosine phosphatase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR006064; Glycosidase.
DR   Pfam; PF02027; RolB_RolC; 1.
PE   4: Predicted;
KW   Hydrolase; Plasmid; Protein phosphatase.
FT   CHAIN           1..259
FT                   /note="Protein-tyrosine phosphatase RolB"
FT                   /id="PRO_0000097391"
FT   REGION          219..259
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   259 AA;  29611 MW;  32A4361EA4946386 CRC64;
     MDPKLLFLPR FQPVDLTPAW SQINLFEGIR FAFAIYSRDY SKPLLHFQKR WALAVLDLKE
     NSPPIYILKQ LAELLKNKVC YHPPMFVSQP DLARENDQHV FVYLSREKMQ KVLKEQSITF
     GMEAVLATTI QPYRSELALQ EMLRVHNLAW PHSRTEEPDL ECFIAIFASS LFIHLLELKV
     TNVYGREVAC TFFLRRGTEN RPYDVVACGT TQFTKNALGI SRPAASSPEP DLTLRLSGPD
     QEGEEGVMKP AAVNLKKEA
//