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Protein

Protein-tyrosine phosphatase RolB

Gene

rolB

Organism
Agrobacterium rhizogenes
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Induces differentiation and growth of neoplastic roots (hairy roots). Seems to function as a tyrosine phosphatase.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-tyrosine phosphatase RolB (EC:3.1.3.48)
Short name:
ROL B protein
Gene namesi
Name:rolB
Encoded oniPlasmid pRiA4b0 Publication
OrganismiAgrobacterium rhizogenes
Taxonomic identifieri359 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 259259Protein-tyrosine phosphatase RolBPRO_0000097391Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR006064. Glycosidase.
[Graphical view]
PfamiPF02027. RolB_RolC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20402-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDPKLLFLPR FQPVDLTPAW SQINLFEGIR FAFAIYSRDY SKPLLHFQKR
60 70 80 90 100
WALAVLDLKE NSPPIYILKQ LAELLKNKVC YHPPMFVSQP DLARENDQHV
110 120 130 140 150
FVYLSREKMQ KVLKEQSITF GMEAVLATTI QPYRSELALQ EMLRVHNLAW
160 170 180 190 200
PHSRTEEPDL ECFIAIFASS LFIHLLELKV TNVYGREVAC TFFLRRGTEN
210 220 230 240 250
RPYDVVACGT TQFTKNALGI SRPAASSPEP DLTLRLSGPD QEGEEGVMKP

AAVNLKKEA
Length:259
Mass (Da):29,611
Last modified:February 1, 1991 - v1
Checksum:i32A4361EA4946386
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03433 Genomic DNA. Translation: CAA27163.1.
PIRiA27259.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03433 Genomic DNA. Translation: CAA27163.1.
PIRiA27259.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR006064. Glycosidase.
[Graphical view]
PfamiPF02027. RolB_RolC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "An Agrobacterium transformation in the evolution of the genus Nicotiana."
    Furner I.J., Huffman G.A., Amasino R.M., Garfinkel D.J., Gordon M.P., Nester E.W.
    Nature 319:422-427(1986)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A4.
  2. "The protein encoded by the rolB plant oncogene hydrolyses indole glucosides."
    Estruch J.J., Schell J., Spena A.
    EMBO J. 10:3125-3128(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY FUNCTION.
  3. Cited for: FUNCTION.

Entry informationi

Entry nameiROB1_AGRRH
AccessioniPrimary (citable) accession number: P20402
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: October 1, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to release indoles from indoxyl-beta-glucosides.1 Publication

Keywords - Technical termi

Plasmid

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.