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P20402 (ROB1_AGRRH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Protein-tyrosine phosphatase RolB

Short name=ROL B protein
EC=3.1.3.48
Gene names
Name:rolB
Encoded onPlasmid pRiA4b
OrganismAgrobacterium rhizogenes
Taxonomic identifier359 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacterium

Protein attributes

Sequence length259 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Induces differentiation and growth of neoplastic roots (hairy roots). Seems to function as a tyrosine phosphatase. Ref.2 Ref.3

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Caution

Was originally (Ref.2) thought to release indoles from indoxyl-beta-glucosides.

Ontologies

Keywords
   Molecular functionHydrolase
Protein phosphatase
   Technical termPlasmid
Gene Ontology (GO)
   Molecular_functionprotein tyrosine phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 259259Protein-tyrosine phosphatase RolB
PRO_0000097391

Sequences

Sequence LengthMass (Da)Tools
P20402 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 32A4361EA4946386

FASTA25929,611
        10         20         30         40         50         60 
MDPKLLFLPR FQPVDLTPAW SQINLFEGIR FAFAIYSRDY SKPLLHFQKR WALAVLDLKE 

        70         80         90        100        110        120 
NSPPIYILKQ LAELLKNKVC YHPPMFVSQP DLARENDQHV FVYLSREKMQ KVLKEQSITF 

       130        140        150        160        170        180 
GMEAVLATTI QPYRSELALQ EMLRVHNLAW PHSRTEEPDL ECFIAIFASS LFIHLLELKV 

       190        200        210        220        230        240 
TNVYGREVAC TFFLRRGTEN RPYDVVACGT TQFTKNALGI SRPAASSPEP DLTLRLSGPD 

       250 
QEGEEGVMKP AAVNLKKEA 

« Hide

References

[1]"An Agrobacterium transformation in the evolution of the genus Nicotiana."
Furner I.J., Huffman G.A., Amasino R.M., Garfinkel D.J., Gordon M.P., Nester E.W.
Nature 319:422-427(1986)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: A4.
[2]"The protein encoded by the rolB plant oncogene hydrolyses indole glucosides."
Estruch J.J., Schell J., Spena A.
EMBO J. 10:3125-3128(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY FUNCTION.
[3]"A plant oncogene as a phosphatase."
Filippini F., Rossi V., Marin O., Trovato M., Constantino P., Downey P.M., Lo Schiavo F., Terzi M.
Nature 379:499-500(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X03433 Genomic DNA. Translation: CAA27163.1.
PIRA27259.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR006064. Glycosidase.
[Graphical view]
PfamPF02027. RolB_RolC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameROB1_AGRRH
AccessionPrimary (citable) accession number: P20402
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: April 16, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program