P20402 (ROB1_AGRRH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-tyrosine phosphatase RolB
Short name=ROL B protein
EC=3.1.3.48

Gene names

Name:

rolB

Encoded on

Plasmid pRiA4b

Organism

Agrobacterium rhizogenes

Taxonomic identifier

359 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Induces differentiation and growth of neoplastic roots (hairy roots). Seems to function as a tyrosine phosphatase. Ref.2 Ref.3
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Caution	Was originally (Ref.2) thought to release indoles from indoxyl-beta-glucosides.

Ontologies

Keywords
Molecular function	Hydrolase Protein phosphatase
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	Protein-tyrosine phosphatase RolB		PRO_0000097391

Sequences

	Sequence		Length	Mass (Da)
	P20402 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 32A4361EA4946386	FASTA	259	29,611
	10 20 30 40 50 60 MDPKLLFLPR FQPVDLTPAW SQINLFEGIR FAFAIYSRDY SKPLLHFQKR WALAVLDLKE 70 80 90 100 110 120 NSPPIYILKQ LAELLKNKVC YHPPMFVSQP DLARENDQHV FVYLSREKMQ KVLKEQSITF 130 140 150 160 170 180 GMEAVLATTI QPYRSELALQ EMLRVHNLAW PHSRTEEPDL ECFIAIFASS LFIHLLELKV 190 200 210 220 230 240 TNVYGREVAC TFFLRRGTEN RPYDVVACGT TQFTKNALGI SRPAASSPEP DLTLRLSGPD 250 QEGEEGVMKP AAVNLKKEA

References

[1]	"An Agrobacterium transformation in the evolution of the genus Nicotiana." Furner I.J., Huffman G.A., Amasino R.M., Garfinkel D.J., Gordon M.P., Nester E.W. Nature 319:422-427(1986) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A4.
[2]	"The protein encoded by the rolB plant oncogene hydrolyses indole glucosides." Estruch J.J., Schell J., Spena A. EMBO J. 10:3125-3128(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PRELIMINARY FUNCTION.
[3]	"A plant oncogene as a phosphatase." Filippini F., Rossi V., Marin O., Trovato M., Constantino P., Downey P.M., Lo Schiavo F., Terzi M. Nature 379:499-500(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03433 Genomic DNA. Translation: CAA27163.1.
PIR	A27259.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR006064. Glycosidase. [Graphical view]
Pfam	PF02027. RolB_RolC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ROB1_AGRRH

Accession

Primary (citable) accession number: P20402

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	April 3, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program