P20382 (MCH_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pro-MCH

Cleaved into the following 3 chains:

1. Neuropeptide-glycine-glutamic acid
   Short name=NGE
   Short name=Neuropeptide G-E
2. Neuropeptide-glutamic acid-isoleucine
   Short name=NEI
   Short name=Neuropeptide E-I
3. Melanin-concentrating hormone
   Short name=MCH

Gene names

Name:	PMCH
Synonyms:	MCH

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. May also have a role in spermatocyte differentiation.
Subcellular location	Secreted.
Tissue specificity	Predominantly expressed in lateral hypothalamus, also detected in pallidum, neocortex and cerebellum. Also found in thymus, brown adipose tissue, duodenum and testis (spermatogonia, early spermatocytes and Sertoli cells). No expression in peripheral blood. In brain exclusively mature MCH and NEI peptides are present. In peripheral tissues a large product, encompassing the NEI and MCH domains of the precursor, is found predominantly. Ref.6
Post-translational modification	Differentially processed in the brain and in peripheral organs producing two neuropeptides; NEI and MCH. A third peptide, NGE, may also be produced. Preferential processing in neurons by prohormone convertase 2 (PC2) generates NEI. MCH is generated in neurons of the lateral hypothalmic area by several prohormone convertases including PC1/3, PC2 and PC5/6.
Sequence similarities	Belongs to the melanin-concentrating hormone family.

Ontologies

Keywords
Biological process	Differentiation Spermatogenesis
Cellular component	Secreted
Domain	Signal
Molecular function	Developmental protein Hormone Neuropeptide
PTM	Amidation Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW feeding behavior Inferred from sequence or structural similarity. Source: UniProtKB multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW neuropeptide signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Non-traceable author statement PubMed 1572663. Source: ProtInc spermatogenesis Non-traceable author statement. Source: UniProtKB synaptic transmission Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Non-traceable author statement Ref.2. Source: UniProtKB nucleus Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 165	144	Pro-MCH		PRO_0000019104
	Peptide	110 – 128	19	Neuropeptide-glycine-glutamic acid Potential		PRO_0000019105
	Peptide	131 – 143	13	Neuropeptide-glutamic acid-isoleucine		PRO_0000019106
	Peptide	147 – 165	19	Melanin-concentrating hormone		PRO_0000019107
Amino acid modifications
	Modified residue	143	1	Isoleucine amide By similarity
	Disulfide bond	153 ↔ 162		By similarity
Experimental info
	Sequence conflict	42	1	F → S in AAB27493. Ref.2
	Sequence conflict	104 – 106	3	PYL → GYQ in AAA63214. Ref.1
	Sequence conflict	113	1	A → D in AAA63214. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P20382 [UniParc]. Last modified May 5, 2009. Version 3. Checksum: 2339E8938EBEC499	FASTA	165	18,679
	10 20 30 40 50 60 MAKMNLSSYI LILTFSLFSQ GILLSASKSI RNLDDDMVFN TFRLGKGFQK EDTAEKSVIA 70 80 90 100 110 120 PSLEQYKNDE SSFMNEEENK VSKNTGSKHN FLNHGLPLNL AIKPYLALKG SVAFPAENGV 130 140 150 160 QNTESTQEKR EIGDEENSAK FPIGRRDFDM LRCMLGRVYR PCWQV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the human melanin concentrating hormone mRNA." Presse F., Nahon J.-L., Fischer W.H., Vale W. Mol. Endocrinol. 4:632-637(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Hypothalamus.
[2]	"Isolation and characterization of the human melanin-concentrating hormone gene and a variant gene." Breton C., Schorpp M., Nahon J.-L. Brain Res. Mol. Brain Res. 18:297-310(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Mammary cancer.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Cellular localization and role of prohormone convertases in the processing of pro-melanin concentrating hormone in mammals." Viale A., Ortola C., Hervieu G., Furuta M., Barbero P., Steiner D.F., Seidah N.G., Nahon J.-L. J. Biol. Chem. 274:6536-6545(1999) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEOLYTIC PROCESSING.
[6]	"The melanin-concentrating hormone gene in human: flanking region analysis, fine chromosome mapping, and tissue-specific expression." Viale A., Zhixing Y., Breton C., Pedeutour F., Coquerel A., Jordan D., Nahon J.-L. Brain Res. Mol. Brain Res. 46:243-255(1997) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M57703 mRNA. Translation: AAA63214.1. S63697 Genomic DNA. Translation: AAB27493.1. CH471054 Genomic DNA. Translation: EAW97693.1. BC018048 mRNA. Translation: AAH18048.1.
IPI	IPI00300623.
PIR	I52634.
RefSeq	NP_002665.2. NM_002674.2.
UniGene	Hs.707990.
3D structure databases
ProteinModelPortal	P20382.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000332225.
Polymorphism databases
DMDM	229462960.
Proteomic databases
PaxDb	P20382.
PRIDE	P20382.
Protocols and materials databases
DNASU	5367.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000329406; ENSP00000332225; ENSG00000183395.
GeneID	5367.
KEGG	hsa:5367.
UCSC	uc001tjl.3. human.
Organism-specific databases
CTD	5367.
GeneCards	GC12M102590.
HGNC	HGNC:9109. PMCH.
HPA	HPA046055.
MIM	176795. gene.
neXtProt	NX_P20382.
PharmGKB	PA33435.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG42445.
HOGENOM	HOG000230520.
HOVERGEN	HBG052429.
InParanoid	P20382.
KO	K05229.
OMA	IGDEENS.
OrthoDB	EOG46MBM5.
PhylomeDB	P20382.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
Bgee	P20382.
CleanEx	HS_PMCH.
Genevestigator	P20382.
GermOnline	ENSG00000183395. Homo sapiens.
Family and domain databases
InterPro	IPR005456. Prepro-melanin_conc_hormone. [Graphical view]
PANTHER	PTHR12091. PTHR12091. 1 hit.
Pfam	PF05824. Pro-MCH. 1 hit. [Graphical view]
PRINTS	PR01641. PROMCHFAMILY.
ProtoNet	Search...
Other
GenomeRNAi	5367.
NextBio	20804.
PMAP-CutDB	Q8WVG0.
SOURCE	Search...

Entry information

Entry name

MCH_HUMAN

Accession

Primary (citable) accession number: P20382
Secondary accession number(s): Q16044, Q8WVG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	May 5, 2009
Last modified:	April 3, 2013
This is version 117 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.