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Protein

Cytochrome c oxidase subunit 1

Gene

COI

Organism
Apis mellifera ligustica (Common honeybee) (Italian honeybee)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.

Catalytic activityi

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Pathway:ioxidative phosphorylation

This protein is involved in the pathway oxidative phosphorylation, which is part of Energy metabolism.
View all proteins of this organism that are known to be involved in the pathway oxidative phosphorylation and in Energy metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi59 – 591Iron (heme A axial ligand)Curated
Metal bindingi238 – 2381Copper BCurated
Metal bindingi242 – 2421Copper BCurated
Metal bindingi288 – 2881Copper BCurated
Metal bindingi289 – 2891Copper BCurated
Metal bindingi374 – 3741Iron (heme A3 axial ligand)Curated
Metal bindingi376 – 3761Iron (heme A axial ligand)Curated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Copper, Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00705.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c oxidase subunit 1 (EC:1.9.3.1)
Alternative name(s):
Cytochrome c oxidase polypeptide I
Gene namesi
Name:COI
Encoded oniMitochondrion
OrganismiApis mellifera ligustica (Common honeybee) (Italian honeybee)
Taxonomic identifieri7469 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataApoideaApidaeApis

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei15 – 3521HelicalSequence AnalysisAdd
BLAST
Transmembranei61 – 8121HelicalSequence AnalysisAdd
BLAST
Transmembranei98 – 11821HelicalSequence AnalysisAdd
BLAST
Transmembranei143 – 16321HelicalSequence AnalysisAdd
BLAST
Transmembranei184 – 20421HelicalSequence AnalysisAdd
BLAST
Transmembranei232 – 25221HelicalSequence AnalysisAdd
BLAST
Transmembranei265 – 28521HelicalSequence AnalysisAdd
BLAST
Transmembranei303 – 32321HelicalSequence AnalysisAdd
BLAST
Transmembranei336 – 35621HelicalSequence AnalysisAdd
BLAST
Transmembranei383 – 40321HelicalSequence AnalysisAdd
BLAST
Transmembranei412 – 43221HelicalSequence AnalysisAdd
BLAST
Transmembranei451 – 47121HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 521521Cytochrome c oxidase subunit 1PRO_0000183284Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki238 ↔ 2421'-histidyl-3'-tyrosine (His-Tyr)By similarity

Structurei

3D structure databases

ProteinModelPortaliP20374.
SMRiP20374. Positions 3-504.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
InterProiIPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
[Graphical view]
PRINTSiPR01165. CYCOXIDASEI.
SUPFAMiSSF81442. SSF81442. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20374-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKWFMSTNH KNIGILYIIL ALWSGMLGSS MSLIIRMELS SPGSWISNDQ
60 70 80 90 100
IYNTIVTSHA FLMIFFMVMP FLIGGFGNWL IPLMLGSPDM AFPRMNNISF
110 120 130 140 150
WLLPPSLFML LLSNLFYPSP GTGWTVYPPL SAYLYHSSPS VDFAIFSLHM
160 170 180 190 200
SGISSIMGSL NLMVTIMMMK NFSMNYDQIS LFPWSVFITA ILLIMSLPVL
210 220 230 240 250
AGAITMLLFD RNFNTSFFDP MGGGDPILYQ HLFWFFGHPE VYILILPGFG
260 270 280 290 300
LISHIVMNES GKKEIFGNLS MIYAMLGIGF LGFIVWAHHM FTVGLDVDTR
310 320 330 340 350
AYFTSATMII AVPTGIKVFS WLATYHGSKL KLNISILWSL GFIMLFTIGG
360 370 380 390 400
LTGIMLSNSS IDIILHDTYY VVGHFHYVLS MGAVFAIISS FIHWYPLITG
410 420 430 440 450
LLLNIKWLKI QFIMMFIGVN LTFFPQHFLG LMSMPRRYSD YPDSYYCWNS
460 470 480 490 500
ISSMGSMISL NSMIFLIFII LESLISKRML LFKFNQSSLE WLNFLPPLDH
510 520
SHLEIPLLIK NLNLKSILIK F
Length:521
Mass (Da):59,294
Last modified:February 1, 1991 - v1
Checksum:i2149417AC981CE64
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti108 – 1081F → L in strain: Isolate ligus8.
Natural varianti270 – 2701S → G in strain: Isolate ligus8.
Natural varianti271 – 2711M → I in strain: Isolate ligus2.
Natural varianti437 – 4371R → P in strain: Isolate ligus7.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23409 Genomic DNA. Translation: AAA18476.1.
L06178 Genomic DNA. Translation: AAB96799.1.
AY114452 Genomic DNA. Translation: AAM76437.1.
AY114453 Genomic DNA. Translation: AAM76438.1.
AY114454 Genomic DNA. Translation: AAM76439.1.
AY114455 Genomic DNA. Translation: AAM76440.1.
AY114456 Genomic DNA. Translation: AAM76441.1.
AY114457 Genomic DNA. Translation: AAM76442.1.
AY114458 Genomic DNA. Translation: AAM76443.1.
AY114460 Genomic DNA. Translation: AAM76445.1.
PIRiA32431.
RefSeqiNP_008083.1. NC_001566.1.

Genome annotation databases

GeneIDi807695.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23409 Genomic DNA. Translation: AAA18476.1.
L06178 Genomic DNA. Translation: AAB96799.1.
AY114452 Genomic DNA. Translation: AAM76437.1.
AY114453 Genomic DNA. Translation: AAM76438.1.
AY114454 Genomic DNA. Translation: AAM76439.1.
AY114455 Genomic DNA. Translation: AAM76440.1.
AY114456 Genomic DNA. Translation: AAM76441.1.
AY114457 Genomic DNA. Translation: AAM76442.1.
AY114458 Genomic DNA. Translation: AAM76443.1.
AY114460 Genomic DNA. Translation: AAM76445.1.
PIRiA32431.
RefSeqiNP_008083.1. NC_001566.1.

3D structure databases

ProteinModelPortaliP20374.
SMRiP20374. Positions 3-504.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi807695.

Organism-specific databases

CTDi4512.

Enzyme and pathway databases

UniPathwayiUPA00705.

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
InterProiIPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
[Graphical view]
PRINTSiPR01165. CYCOXIDASEI.
SUPFAMiSSF81442. SSF81442. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The CO-I and CO-II region of honeybee mitochondrial DNA: evidence for variation in insect mitochondrial evolutionary rates."
    Crozier R.H., Crozier Y.C., Mackinlay A.G.
    Mol. Biol. Evol. 6:399-411(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Thorax.
  2. "The mitochondrial genome of the honeybee Apis mellifera: complete sequence and genome organization."
    Crozier R.H., Crozier Y.C.
    Genetics 133:97-117(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Thorax.
  3. "ND2 and CO1 mitochondrial genes in Apis mellifera L.: a molecular approach to Mediterranean populations monitoring."
    Marino A., Mantovani B., Carpana E., Sabatini A.G., Lodesani M.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 93-507.
    Strain: Isolate ligus2, Isolate ligus3, Isolate ligus4, Isolate ligus5, Isolate ligus6, Isolate ligus7, Isolate ligus8 and Isolate ligus9.
  4. "Putative origin and function of the intergenic region between COI and COII of Apis mellifera L. mitochondrial DNA."
    Cornuet J.-M., Garnery L., Solignac M.
    Genetics 128:393-403(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 338-521.

Entry informationi

Entry nameiCOX1_APILI
AccessioniPrimary (citable) accession number: P20374
Secondary accession number(s): Q8LUG0
, Q8LXP4, Q8LXP6, Q8LXP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: January 7, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.