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P20372 (PCXB_ACIAD) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protocatechuate 3,4-dioxygenase beta chain

EC=1.13.11.3
Alternative name(s):
3,4-PCD
Gene names
Name:pcaH
Ordered Locus Names:ACIAD1711
OrganismAcinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) [Complete proteome] [HAMAP]
Taxonomic identifier62977 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter

Protein attributes

Sequence length241 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway.

Catalytic activity

3,4-dihydroxybenzoate + O2 = 3-carboxy-cis,cis-muconate.

Cofactor

Binds Fe3+ ion per subunit.

Pathway

Aromatic compound metabolism; beta-ketoadipate pathway; 3-carboxy-cis,cis-muconate from 3,4-dihydroxybenzoate: step 1/1.

Subunit structure

The enzyme is an oligomer of 12 copies of the alpha and beta chains.

Sequence similarities

Belongs to the intradiol ring-cleavage dioxygenase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

pcaGP203715EBI-1029420,EBI-1029428

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 241241Protocatechuate 3,4-dioxygenase beta chain
PRO_0000085097

Sites

Metal binding1091Iron; catalytic By similarity
Metal binding1481Iron; catalytic By similarity
Metal binding1611Iron; catalytic By similarity
Metal binding1631Iron; catalytic By similarity

Secondary structure

..................................................... 241
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P20372 [UniParc].

Last modified July 15, 1999. Version 2.
Checksum: 0D2F945C92FE675A

FASTA24127,548
        10         20         30         40         50         60 
MSQIIWGAYA QRNTEDHPPA YAPGYKTSVL RSPKNALISI AETLSEVTAP HFSADKFGPK 

        70         80         90        100        110        120 
DNDLILNYAK DGLPIGERVI VHGYVRDQFG RPVKNALVEV WQANASGRYR HPNDQYIGAM 

       130        140        150        160        170        180 
DPNFGGCGRM LTDDNGYYVF RTIKPGPYPW RNRINEWRPA HIHFSLIADG WAQRLISQFY 

       190        200        210        220        230        240 
FEGDTLIDSC PILKTIPSEQ QRRALIALED KSNFIEADSR CYRFDITLRG RRATYFENDL 


T 

« Hide

References

« Hide 'large scale' references
[1]"DNA sequences of genes encoding Acinetobacter calcoaceticus protocatechuate 3,4-dioxygenase: evidence indicating shuffling of genes and of DNA sequences within genes during their evolutionary divergence."
Hartnett C., Neidle E.L., Ngai K.-L., Ornston L.N.
J. Bacteriol. 172:956-966(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
[2]"Contrasting patterns of evolutionary divergence within the Acinetobacter calcoaceticus pca operon."
Kowalchuk G.A., Hartnett G.B., Benson A., Houghton J.E., Ngai K.-L., Ornston L.N.
Gene 146:23-30(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 12; 22; 36 AND 232-241.
[3]"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium."
Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C.
Nucleic Acids Res. 32:5766-5779(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33305 / BD413 / ADP1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L05770 Genomic DNA. Translation: AAC37153.1.
CR543861 Genomic DNA. Translation: CAG68553.1.
PIRC35119.
RefSeqYP_046375.1. NC_005966.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EO2X-ray2.25B1-241[»]
1EO9X-ray2.00B1-241[»]
1EOAX-ray2.15B1-241[»]
1EOBX-ray2.20B1-241[»]
1EOCX-ray2.25B1-241[»]
2BUMX-ray1.80B1-241[»]
2BUQX-ray1.80B1-241[»]
2BURX-ray1.80B1-241[»]
2BUTX-ray1.85B1-241[»]
2BUUX-ray1.80B1-241[»]
2BUVX-ray1.80B1-241[»]
2BUWX-ray1.80B1-241[»]
2BUXX-ray1.80B1-241[»]
2BUYX-ray1.80B1-241[»]
2BUZX-ray1.80B1-241[»]
2BV0X-ray1.80B1-241[»]
ProteinModelPortalP20372.
SMRP20372. Positions 4-241.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP20372. 1 interaction.
MINTMINT-1692757.
STRING62977.ACIAD1711.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAG68553; CAG68553; ACIAD1711.
GeneID2879437.
KEGGaci:ACIAD1711.
PATRIC20741192. VBIAciSp98416_1542.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3485.
HOGENOMHOG000174837.
KOK00449.
OMAFAPGYKT.
OrthoDBEOG60PHG0.

Enzyme and pathway databases

BioCycASP62977:GJVV-1623-MONOMER.
UniPathwayUPA00157; UER00264.

Family and domain databases

Gene3D2.60.130.10. 1 hit.
InterProIPR000627. Intradiol_dOase_C.
IPR015889. Intradiol_dOase_core.
IPR024756. PCDO_beta_N.
IPR012785. Protocat_dOase_b.
[Graphical view]
PfamPF00775. Dioxygenase_C. 1 hit.
PF12391. PCDO_beta_N. 1 hit.
[Graphical view]
SUPFAMSSF49482. SSF49482. 1 hit.
TIGRFAMsTIGR02422. protocat_beta. 1 hit.
PROSITEPS00083. INTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP20372.

Entry information

Entry namePCXB_ACIAD
AccessionPrimary (citable) accession number: P20372
Secondary accession number(s): Q43976
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: July 15, 1999
Last modified: June 11, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways