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Protein

Protocatechuate 3,4-dioxygenase beta chain

Gene

pcaH

Organism
Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway.

Catalytic activityi

3,4-dihydroxybenzoate + O2 = 3-carboxy-cis,cis-muconate.

Cofactori

Fe3+Note: Binds Fe3+ ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi109 – 1091Iron; catalyticBy similarity
Metal bindingi148 – 1481Iron; catalyticBy similarity
Metal bindingi161 – 1611Iron; catalyticBy similarity
Metal bindingi163 – 1631Iron; catalyticBy similarity

GO - Molecular functioni

  1. ferric iron binding Source: InterPro
  2. protocatechuate 3,4-dioxygenase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-ketoadipate pathway Source: UniProtKB-UniPathway
  2. protocatechuate catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciASP62977:GJVV-1623-MONOMER.
UniPathwayiUPA00157; UER00264.

Names & Taxonomyi

Protein namesi
Recommended name:
Protocatechuate 3,4-dioxygenase beta chain (EC:1.13.11.3)
Alternative name(s):
3,4-PCD
Gene namesi
Name:pcaH
Ordered Locus Names:ACIAD1711
OrganismiAcinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Taxonomic identifieri62977 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter
ProteomesiUP000000430: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 241241Protocatechuate 3,4-dioxygenase beta chainPRO_0000085097Add
BLAST

Interactioni

Subunit structurei

The enzyme is an oligomer of 12 copies of the alpha and beta chains.

Binary interactionsi

WithEntry#Exp.IntActNotes
pcaGP203715EBI-1029420,EBI-1029428

Protein-protein interaction databases

IntActiP20372. 1 interaction.
MINTiMINT-1692757.
STRINGi62977.ACIAD1711.

Structurei

Secondary structure

1
241
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 95Combined sources
Helixi14 – 163Combined sources
Helixi26 – 294Combined sources
Beta strandi30 – 323Combined sources
Helixi44 – 474Combined sources
Helixi54 – 563Combined sources
Turni59 – 624Combined sources
Turni64 – 685Combined sources
Beta strandi70 – 723Combined sources
Beta strandi78 – 8710Combined sources
Beta strandi97 – 1015Combined sources
Beta strandi127 – 1315Combined sources
Beta strandi136 – 1438Combined sources
Beta strandi148 – 1536Combined sources
Beta strandi156 – 1583Combined sources
Beta strandi160 – 1678Combined sources
Helixi171 – 1733Combined sources
Beta strandi175 – 1817Combined sources
Helixi185 – 1895Combined sources
Helixi193 – 1953Combined sources
Helixi199 – 2035Combined sources
Beta strandi206 – 2094Combined sources
Helixi211 – 2133Combined sources
Turni216 – 2183Combined sources
Beta strandi219 – 2235Combined sources
Beta strandi226 – 2283Combined sources
Beta strandi231 – 2366Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EO2X-ray2.25B1-241[»]
1EO9X-ray2.00B1-241[»]
1EOAX-ray2.15B1-241[»]
1EOBX-ray2.20B1-241[»]
1EOCX-ray2.25B1-241[»]
2BUMX-ray1.80B1-241[»]
2BUQX-ray1.80B1-241[»]
2BURX-ray1.80B1-241[»]
2BUTX-ray1.85B1-241[»]
2BUUX-ray1.80B1-241[»]
2BUVX-ray1.80B1-241[»]
2BUWX-ray1.80B1-241[»]
2BUXX-ray1.80B1-241[»]
2BUYX-ray1.80B1-241[»]
2BUZX-ray1.80B1-241[»]
2BV0X-ray1.80B1-241[»]
ProteinModelPortaliP20372.
SMRiP20372. Positions 4-241.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20372.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3485.
HOGENOMiHOG000174837.
KOiK00449.
OMAiAYKFDIV.
OrthoDBiEOG60PHG0.

Family and domain databases

Gene3Di2.60.130.10. 1 hit.
InterProiIPR000627. Intradiol_dOase_C.
IPR015889. Intradiol_dOase_core.
IPR024756. PCDO_beta_N.
IPR012785. Protocat_dOase_b.
[Graphical view]
PfamiPF00775. Dioxygenase_C. 1 hit.
PF12391. PCDO_beta_N. 1 hit.
[Graphical view]
SUPFAMiSSF49482. SSF49482. 1 hit.
TIGRFAMsiTIGR02422. protocat_beta. 1 hit.
PROSITEiPS00083. INTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20372-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQIIWGAYA QRNTEDHPPA YAPGYKTSVL RSPKNALISI AETLSEVTAP
60 70 80 90 100
HFSADKFGPK DNDLILNYAK DGLPIGERVI VHGYVRDQFG RPVKNALVEV
110 120 130 140 150
WQANASGRYR HPNDQYIGAM DPNFGGCGRM LTDDNGYYVF RTIKPGPYPW
160 170 180 190 200
RNRINEWRPA HIHFSLIADG WAQRLISQFY FEGDTLIDSC PILKTIPSEQ
210 220 230 240
QRRALIALED KSNFIEADSR CYRFDITLRG RRATYFENDL T
Length:241
Mass (Da):27,548
Last modified:July 15, 1999 - v2
Checksum:i0D2F945C92FE675A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05770 Genomic DNA. Translation: AAC37153.1.
CR543861 Genomic DNA. Translation: CAG68553.1.
PIRiC35119.
RefSeqiYP_046375.1. NC_005966.1.

Genome annotation databases

EnsemblBacteriaiCAG68553; CAG68553; ACIAD1711.
GeneIDi2879437.
KEGGiaci:ACIAD1711.
PATRICi20741192. VBIAciSp98416_1542.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05770 Genomic DNA. Translation: AAC37153.1.
CR543861 Genomic DNA. Translation: CAG68553.1.
PIRiC35119.
RefSeqiYP_046375.1. NC_005966.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EO2X-ray2.25B1-241[»]
1EO9X-ray2.00B1-241[»]
1EOAX-ray2.15B1-241[»]
1EOBX-ray2.20B1-241[»]
1EOCX-ray2.25B1-241[»]
2BUMX-ray1.80B1-241[»]
2BUQX-ray1.80B1-241[»]
2BURX-ray1.80B1-241[»]
2BUTX-ray1.85B1-241[»]
2BUUX-ray1.80B1-241[»]
2BUVX-ray1.80B1-241[»]
2BUWX-ray1.80B1-241[»]
2BUXX-ray1.80B1-241[»]
2BUYX-ray1.80B1-241[»]
2BUZX-ray1.80B1-241[»]
2BV0X-ray1.80B1-241[»]
ProteinModelPortaliP20372.
SMRiP20372. Positions 4-241.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP20372. 1 interaction.
MINTiMINT-1692757.
STRINGi62977.ACIAD1711.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG68553; CAG68553; ACIAD1711.
GeneIDi2879437.
KEGGiaci:ACIAD1711.
PATRICi20741192. VBIAciSp98416_1542.

Phylogenomic databases

eggNOGiCOG3485.
HOGENOMiHOG000174837.
KOiK00449.
OMAiAYKFDIV.
OrthoDBiEOG60PHG0.

Enzyme and pathway databases

UniPathwayiUPA00157; UER00264.
BioCyciASP62977:GJVV-1623-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP20372.

Family and domain databases

Gene3Di2.60.130.10. 1 hit.
InterProiIPR000627. Intradiol_dOase_C.
IPR015889. Intradiol_dOase_core.
IPR024756. PCDO_beta_N.
IPR012785. Protocat_dOase_b.
[Graphical view]
PfamiPF00775. Dioxygenase_C. 1 hit.
PF12391. PCDO_beta_N. 1 hit.
[Graphical view]
SUPFAMiSSF49482. SSF49482. 1 hit.
TIGRFAMsiTIGR02422. protocat_beta. 1 hit.
PROSITEiPS00083. INTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequences of genes encoding Acinetobacter calcoaceticus protocatechuate 3,4-dioxygenase: evidence indicating shuffling of genes and of DNA sequences within genes during their evolutionary divergence."
    Hartnett C., Neidle E.L., Ngai K.-L., Ornston L.N.
    J. Bacteriol. 172:956-966(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
  2. "Contrasting patterns of evolutionary divergence within the Acinetobacter calcoaceticus pca operon."
    Kowalchuk G.A., Hartnett G.B., Benson A., Houghton J.E., Ngai K.-L., Ornston L.N.
    Gene 146:23-30(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 12; 22; 36 AND 232-241.
  3. "Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium."
    Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C.
    Nucleic Acids Res. 32:5766-5779(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33305 / BD413 / ADP1.

Entry informationi

Entry nameiPCXB_ACIAD
AccessioniPrimary (citable) accession number: P20372
Secondary accession number(s): Q43976
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: July 15, 1999
Last modified: January 7, 2015
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.