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P20372

- PCXB_ACIAD

UniProt

P20372 - PCXB_ACIAD

Protein

Protocatechuate 3,4-dioxygenase beta chain

Gene

pcaH

Organism
Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (15 Jul 1999)
      Previous versions | rss
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    Functioni

    Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway.

    Catalytic activityi

    3,4-dihydroxybenzoate + O2 = 3-carboxy-cis,cis-muconate.

    Cofactori

    Binds Fe3+ ion per subunit.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi109 – 1091Iron; catalyticBy similarity
    Metal bindingi148 – 1481Iron; catalyticBy similarity
    Metal bindingi161 – 1611Iron; catalyticBy similarity
    Metal bindingi163 – 1631Iron; catalyticBy similarity

    GO - Molecular functioni

    1. ferric iron binding Source: InterPro
    2. protein binding Source: IntAct
    3. protocatechuate 3,4-dioxygenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-ketoadipate pathway Source: UniProtKB-UniPathway
    2. protocatechuate catabolic process Source: InterPro

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Biological processi

    Aromatic hydrocarbons catabolism

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciASP62977:GJVV-1623-MONOMER.
    UniPathwayiUPA00157; UER00264.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protocatechuate 3,4-dioxygenase beta chain (EC:1.13.11.3)
    Alternative name(s):
    3,4-PCD
    Gene namesi
    Name:pcaH
    Ordered Locus Names:ACIAD1711
    OrganismiAcinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
    Taxonomic identifieri62977 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter
    ProteomesiUP000000430: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 241241Protocatechuate 3,4-dioxygenase beta chainPRO_0000085097Add
    BLAST

    Interactioni

    Subunit structurei

    The enzyme is an oligomer of 12 copies of the alpha and beta chains.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    pcaGP203715EBI-1029420,EBI-1029428

    Protein-protein interaction databases

    IntActiP20372. 1 interaction.
    MINTiMINT-1692757.
    STRINGi62977.ACIAD1711.

    Structurei

    Secondary structure

    1
    241
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 95
    Helixi14 – 163
    Helixi26 – 294
    Beta strandi30 – 323
    Helixi44 – 474
    Helixi54 – 563
    Turni59 – 624
    Turni64 – 685
    Beta strandi70 – 723
    Beta strandi78 – 8710
    Beta strandi97 – 1015
    Beta strandi127 – 1315
    Beta strandi136 – 1438
    Beta strandi148 – 1536
    Beta strandi156 – 1583
    Beta strandi160 – 1678
    Helixi171 – 1733
    Beta strandi175 – 1817
    Helixi185 – 1895
    Helixi193 – 1953
    Helixi199 – 2035
    Beta strandi206 – 2094
    Helixi211 – 2133
    Turni216 – 2183
    Beta strandi219 – 2235
    Beta strandi226 – 2283
    Beta strandi231 – 2366

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1EO2X-ray2.25B1-241[»]
    1EO9X-ray2.00B1-241[»]
    1EOAX-ray2.15B1-241[»]
    1EOBX-ray2.20B1-241[»]
    1EOCX-ray2.25B1-241[»]
    2BUMX-ray1.80B1-241[»]
    2BUQX-ray1.80B1-241[»]
    2BURX-ray1.80B1-241[»]
    2BUTX-ray1.85B1-241[»]
    2BUUX-ray1.80B1-241[»]
    2BUVX-ray1.80B1-241[»]
    2BUWX-ray1.80B1-241[»]
    2BUXX-ray1.80B1-241[»]
    2BUYX-ray1.80B1-241[»]
    2BUZX-ray1.80B1-241[»]
    2BV0X-ray1.80B1-241[»]
    ProteinModelPortaliP20372.
    SMRiP20372. Positions 4-241.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP20372.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG3485.
    HOGENOMiHOG000174837.
    KOiK00449.
    OMAiFAPGYKT.
    OrthoDBiEOG60PHG0.

    Family and domain databases

    Gene3Di2.60.130.10. 1 hit.
    InterProiIPR000627. Intradiol_dOase_C.
    IPR015889. Intradiol_dOase_core.
    IPR024756. PCDO_beta_N.
    IPR012785. Protocat_dOase_b.
    [Graphical view]
    PfamiPF00775. Dioxygenase_C. 1 hit.
    PF12391. PCDO_beta_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49482. SSF49482. 1 hit.
    TIGRFAMsiTIGR02422. protocat_beta. 1 hit.
    PROSITEiPS00083. INTRADIOL_DIOXYGENAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P20372-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQIIWGAYA QRNTEDHPPA YAPGYKTSVL RSPKNALISI AETLSEVTAP    50
    HFSADKFGPK DNDLILNYAK DGLPIGERVI VHGYVRDQFG RPVKNALVEV 100
    WQANASGRYR HPNDQYIGAM DPNFGGCGRM LTDDNGYYVF RTIKPGPYPW 150
    RNRINEWRPA HIHFSLIADG WAQRLISQFY FEGDTLIDSC PILKTIPSEQ 200
    QRRALIALED KSNFIEADSR CYRFDITLRG RRATYFENDL T 241
    Length:241
    Mass (Da):27,548
    Last modified:July 15, 1999 - v2
    Checksum:i0D2F945C92FE675A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L05770 Genomic DNA. Translation: AAC37153.1.
    CR543861 Genomic DNA. Translation: CAG68553.1.
    PIRiC35119.
    RefSeqiYP_046375.1. NC_005966.1.

    Genome annotation databases

    EnsemblBacteriaiCAG68553; CAG68553; ACIAD1711.
    GeneIDi2879437.
    KEGGiaci:ACIAD1711.
    PATRICi20741192. VBIAciSp98416_1542.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L05770 Genomic DNA. Translation: AAC37153.1 .
    CR543861 Genomic DNA. Translation: CAG68553.1 .
    PIRi C35119.
    RefSeqi YP_046375.1. NC_005966.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1EO2 X-ray 2.25 B 1-241 [» ]
    1EO9 X-ray 2.00 B 1-241 [» ]
    1EOA X-ray 2.15 B 1-241 [» ]
    1EOB X-ray 2.20 B 1-241 [» ]
    1EOC X-ray 2.25 B 1-241 [» ]
    2BUM X-ray 1.80 B 1-241 [» ]
    2BUQ X-ray 1.80 B 1-241 [» ]
    2BUR X-ray 1.80 B 1-241 [» ]
    2BUT X-ray 1.85 B 1-241 [» ]
    2BUU X-ray 1.80 B 1-241 [» ]
    2BUV X-ray 1.80 B 1-241 [» ]
    2BUW X-ray 1.80 B 1-241 [» ]
    2BUX X-ray 1.80 B 1-241 [» ]
    2BUY X-ray 1.80 B 1-241 [» ]
    2BUZ X-ray 1.80 B 1-241 [» ]
    2BV0 X-ray 1.80 B 1-241 [» ]
    ProteinModelPortali P20372.
    SMRi P20372. Positions 4-241.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P20372. 1 interaction.
    MINTi MINT-1692757.
    STRINGi 62977.ACIAD1711.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAG68553 ; CAG68553 ; ACIAD1711 .
    GeneIDi 2879437.
    KEGGi aci:ACIAD1711.
    PATRICi 20741192. VBIAciSp98416_1542.

    Phylogenomic databases

    eggNOGi COG3485.
    HOGENOMi HOG000174837.
    KOi K00449.
    OMAi FAPGYKT.
    OrthoDBi EOG60PHG0.

    Enzyme and pathway databases

    UniPathwayi UPA00157 ; UER00264 .
    BioCyci ASP62977:GJVV-1623-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P20372.

    Family and domain databases

    Gene3Di 2.60.130.10. 1 hit.
    InterProi IPR000627. Intradiol_dOase_C.
    IPR015889. Intradiol_dOase_core.
    IPR024756. PCDO_beta_N.
    IPR012785. Protocat_dOase_b.
    [Graphical view ]
    Pfami PF00775. Dioxygenase_C. 1 hit.
    PF12391. PCDO_beta_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49482. SSF49482. 1 hit.
    TIGRFAMsi TIGR02422. protocat_beta. 1 hit.
    PROSITEi PS00083. INTRADIOL_DIOXYGENAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequences of genes encoding Acinetobacter calcoaceticus protocatechuate 3,4-dioxygenase: evidence indicating shuffling of genes and of DNA sequences within genes during their evolutionary divergence."
      Hartnett C., Neidle E.L., Ngai K.-L., Ornston L.N.
      J. Bacteriol. 172:956-966(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
    2. "Contrasting patterns of evolutionary divergence within the Acinetobacter calcoaceticus pca operon."
      Kowalchuk G.A., Hartnett G.B., Benson A., Houghton J.E., Ngai K.-L., Ornston L.N.
      Gene 146:23-30(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION TO 12; 22; 36 AND 232-241.
    3. "Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium."
      Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C.
      Nucleic Acids Res. 32:5766-5779(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33305 / BD413 / ADP1.

    Entry informationi

    Entry nameiPCXB_ACIAD
    AccessioniPrimary (citable) accession number: P20372
    Secondary accession number(s): Q43976
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: July 15, 1999
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3