SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P20372

- PCXB_ACIAD

UniProt

P20372 - PCXB_ACIAD

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Protocatechuate 3,4-dioxygenase beta chain

Gene
pcaH, ACIAD1711
Organism
Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway.

Catalytic activityi

3,4-dihydroxybenzoate + O2 = 3-carboxy-cis,cis-muconate.

Cofactori

Binds Fe3+ ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi109 – 1091Iron; catalytic By similarity
Metal bindingi148 – 1481Iron; catalytic By similarity
Metal bindingi161 – 1611Iron; catalytic By similarity
Metal bindingi163 – 1631Iron; catalytic By similarity

GO - Molecular functioni

  1. ferric iron binding Source: InterPro
  2. protein binding Source: IntAct
  3. protocatechuate 3,4-dioxygenase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-ketoadipate pathway Source: UniProtKB-UniPathway
  2. protocatechuate catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciASP62977:GJVV-1623-MONOMER.
UniPathwayiUPA00157; UER00264.

Names & Taxonomyi

Protein namesi
Recommended name:
Protocatechuate 3,4-dioxygenase beta chain (EC:1.13.11.3)
Alternative name(s):
3,4-PCD
Gene namesi
Name:pcaH
Ordered Locus Names:ACIAD1711
OrganismiAcinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Taxonomic identifieri62977 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter
ProteomesiUP000000430: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 241241Protocatechuate 3,4-dioxygenase beta chainPRO_0000085097Add
BLAST

Interactioni

Subunit structurei

The enzyme is an oligomer of 12 copies of the alpha and beta chains.

Binary interactionsi

WithEntry#Exp.IntActNotes
pcaGP203715EBI-1029420,EBI-1029428

Protein-protein interaction databases

IntActiP20372. 1 interaction.
MINTiMINT-1692757.
STRINGi62977.ACIAD1711.

Structurei

Secondary structure

1
241
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 95
Helixi14 – 163
Helixi26 – 294
Beta strandi30 – 323
Helixi44 – 474
Helixi54 – 563
Turni59 – 624
Turni64 – 685
Beta strandi70 – 723
Beta strandi78 – 8710
Beta strandi97 – 1015
Beta strandi127 – 1315
Beta strandi136 – 1438
Beta strandi148 – 1536
Beta strandi156 – 1583
Beta strandi160 – 1678
Helixi171 – 1733
Beta strandi175 – 1817
Helixi185 – 1895
Helixi193 – 1953
Helixi199 – 2035
Beta strandi206 – 2094
Helixi211 – 2133
Turni216 – 2183
Beta strandi219 – 2235
Beta strandi226 – 2283
Beta strandi231 – 2366

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EO2X-ray2.25B1-241[»]
1EO9X-ray2.00B1-241[»]
1EOAX-ray2.15B1-241[»]
1EOBX-ray2.20B1-241[»]
1EOCX-ray2.25B1-241[»]
2BUMX-ray1.80B1-241[»]
2BUQX-ray1.80B1-241[»]
2BURX-ray1.80B1-241[»]
2BUTX-ray1.85B1-241[»]
2BUUX-ray1.80B1-241[»]
2BUVX-ray1.80B1-241[»]
2BUWX-ray1.80B1-241[»]
2BUXX-ray1.80B1-241[»]
2BUYX-ray1.80B1-241[»]
2BUZX-ray1.80B1-241[»]
2BV0X-ray1.80B1-241[»]
ProteinModelPortaliP20372.
SMRiP20372. Positions 4-241.

Miscellaneous databases

EvolutionaryTraceiP20372.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3485.
HOGENOMiHOG000174837.
KOiK00449.
OMAiFAPGYKT.
OrthoDBiEOG60PHG0.

Family and domain databases

Gene3Di2.60.130.10. 1 hit.
InterProiIPR000627. Intradiol_dOase_C.
IPR015889. Intradiol_dOase_core.
IPR024756. PCDO_beta_N.
IPR012785. Protocat_dOase_b.
[Graphical view]
PfamiPF00775. Dioxygenase_C. 1 hit.
PF12391. PCDO_beta_N. 1 hit.
[Graphical view]
SUPFAMiSSF49482. SSF49482. 1 hit.
TIGRFAMsiTIGR02422. protocat_beta. 1 hit.
PROSITEiPS00083. INTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20372-1 [UniParc]FASTAAdd to Basket

« Hide

MSQIIWGAYA QRNTEDHPPA YAPGYKTSVL RSPKNALISI AETLSEVTAP    50
HFSADKFGPK DNDLILNYAK DGLPIGERVI VHGYVRDQFG RPVKNALVEV 100
WQANASGRYR HPNDQYIGAM DPNFGGCGRM LTDDNGYYVF RTIKPGPYPW 150
RNRINEWRPA HIHFSLIADG WAQRLISQFY FEGDTLIDSC PILKTIPSEQ 200
QRRALIALED KSNFIEADSR CYRFDITLRG RRATYFENDL T 241
Length:241
Mass (Da):27,548
Last modified:July 15, 1999 - v2
Checksum:i0D2F945C92FE675A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L05770 Genomic DNA. Translation: AAC37153.1.
CR543861 Genomic DNA. Translation: CAG68553.1.
PIRiC35119.
RefSeqiYP_046375.1. NC_005966.1.

Genome annotation databases

EnsemblBacteriaiCAG68553; CAG68553; ACIAD1711.
GeneIDi2879437.
KEGGiaci:ACIAD1711.
PATRICi20741192. VBIAciSp98416_1542.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L05770 Genomic DNA. Translation: AAC37153.1 .
CR543861 Genomic DNA. Translation: CAG68553.1 .
PIRi C35119.
RefSeqi YP_046375.1. NC_005966.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1EO2 X-ray 2.25 B 1-241 [» ]
1EO9 X-ray 2.00 B 1-241 [» ]
1EOA X-ray 2.15 B 1-241 [» ]
1EOB X-ray 2.20 B 1-241 [» ]
1EOC X-ray 2.25 B 1-241 [» ]
2BUM X-ray 1.80 B 1-241 [» ]
2BUQ X-ray 1.80 B 1-241 [» ]
2BUR X-ray 1.80 B 1-241 [» ]
2BUT X-ray 1.85 B 1-241 [» ]
2BUU X-ray 1.80 B 1-241 [» ]
2BUV X-ray 1.80 B 1-241 [» ]
2BUW X-ray 1.80 B 1-241 [» ]
2BUX X-ray 1.80 B 1-241 [» ]
2BUY X-ray 1.80 B 1-241 [» ]
2BUZ X-ray 1.80 B 1-241 [» ]
2BV0 X-ray 1.80 B 1-241 [» ]
ProteinModelPortali P20372.
SMRi P20372. Positions 4-241.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P20372. 1 interaction.
MINTi MINT-1692757.
STRINGi 62977.ACIAD1711.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAG68553 ; CAG68553 ; ACIAD1711 .
GeneIDi 2879437.
KEGGi aci:ACIAD1711.
PATRICi 20741192. VBIAciSp98416_1542.

Phylogenomic databases

eggNOGi COG3485.
HOGENOMi HOG000174837.
KOi K00449.
OMAi FAPGYKT.
OrthoDBi EOG60PHG0.

Enzyme and pathway databases

UniPathwayi UPA00157 ; UER00264 .
BioCyci ASP62977:GJVV-1623-MONOMER.

Miscellaneous databases

EvolutionaryTracei P20372.

Family and domain databases

Gene3Di 2.60.130.10. 1 hit.
InterProi IPR000627. Intradiol_dOase_C.
IPR015889. Intradiol_dOase_core.
IPR024756. PCDO_beta_N.
IPR012785. Protocat_dOase_b.
[Graphical view ]
Pfami PF00775. Dioxygenase_C. 1 hit.
PF12391. PCDO_beta_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49482. SSF49482. 1 hit.
TIGRFAMsi TIGR02422. protocat_beta. 1 hit.
PROSITEi PS00083. INTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequences of genes encoding Acinetobacter calcoaceticus protocatechuate 3,4-dioxygenase: evidence indicating shuffling of genes and of DNA sequences within genes during their evolutionary divergence."
    Hartnett C., Neidle E.L., Ngai K.-L., Ornston L.N.
    J. Bacteriol. 172:956-966(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
  2. "Contrasting patterns of evolutionary divergence within the Acinetobacter calcoaceticus pca operon."
    Kowalchuk G.A., Hartnett G.B., Benson A., Houghton J.E., Ngai K.-L., Ornston L.N.
    Gene 146:23-30(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 12; 22; 36 AND 232-241.
  3. "Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium."
    Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C.
    Nucleic Acids Res. 32:5766-5779(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33305 / BD413 / ADP1.

Entry informationi

Entry nameiPCXB_ACIAD
AccessioniPrimary (citable) accession number: P20372
Secondary accession number(s): Q43976
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: July 15, 1999
Last modified: June 11, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi