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Protein

Protocatechuate 3,4-dioxygenase beta chain

Gene

pcaH

Organism
Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway.

Catalytic activityi

3,4-dihydroxybenzoate + O2 = 3-carboxy-cis,cis-muconate.

Cofactori

Fe3+Note: Binds Fe3+ ion per subunit.

Pathwayi: beta-ketoadipate pathway

This protein is involved in step 1 of the subpathway that synthesizes 3-carboxy-cis,cis-muconate from 3,4-dihydroxybenzoate.
Proteins known to be involved in this subpathway in this organism are:
  1. Protocatechuate 3,4-dioxygenase alpha chain (pcaG), Protocatechuate 3,4-dioxygenase beta chain (pcaH)
This subpathway is part of the pathway beta-ketoadipate pathway, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3-carboxy-cis,cis-muconate from 3,4-dihydroxybenzoate, the pathway beta-ketoadipate pathway and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi109Iron; catalyticBy similarity1
Metal bindingi148Iron; catalyticBy similarity1
Metal bindingi161Iron; catalyticBy similarity1
Metal bindingi163Iron; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processAromatic hydrocarbons catabolism
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciASP62977:G1GJ8-1586-MONOMER
UniPathwayiUPA00157; UER00264

Names & Taxonomyi

Protein namesi
Recommended name:
Protocatechuate 3,4-dioxygenase beta chain (EC:1.13.11.3)
Alternative name(s):
3,4-PCD
Gene namesi
Name:pcaH
Ordered Locus Names:ACIAD1711
OrganismiAcinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Taxonomic identifieri62977 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter
Proteomesi
  • UP000000430 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000850971 – 241Protocatechuate 3,4-dioxygenase beta chainAdd BLAST241

Interactioni

Subunit structurei

The enzyme is an oligomer of 12 copies of the alpha and beta chains.

Binary interactionsi

WithEntry#Exp.IntActNotes
pcaGP203715EBI-1029420,EBI-1029428

Protein-protein interaction databases

IntActiP20372, 1 interactor
STRINGi62977.ACIAD1711

Structurei

Secondary structure

1241
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 9Combined sources5
Helixi14 – 16Combined sources3
Helixi26 – 29Combined sources4
Beta strandi30 – 32Combined sources3
Helixi44 – 47Combined sources4
Helixi54 – 56Combined sources3
Turni59 – 62Combined sources4
Turni64 – 68Combined sources5
Beta strandi70 – 72Combined sources3
Beta strandi78 – 87Combined sources10
Beta strandi97 – 101Combined sources5
Beta strandi127 – 131Combined sources5
Beta strandi136 – 143Combined sources8
Beta strandi148 – 153Combined sources6
Beta strandi156 – 158Combined sources3
Beta strandi160 – 167Combined sources8
Helixi171 – 173Combined sources3
Beta strandi175 – 181Combined sources7
Helixi185 – 189Combined sources5
Helixi193 – 195Combined sources3
Helixi199 – 203Combined sources5
Beta strandi206 – 209Combined sources4
Helixi211 – 213Combined sources3
Turni216 – 218Combined sources3
Beta strandi219 – 223Combined sources5
Beta strandi226 – 228Combined sources3
Beta strandi231 – 236Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EO2X-ray2.25B1-241[»]
1EO9X-ray2.00B1-241[»]
1EOAX-ray2.15B1-241[»]
1EOBX-ray2.20B1-241[»]
1EOCX-ray2.25B1-241[»]
2BUMX-ray1.80B1-241[»]
2BUQX-ray1.80B1-241[»]
2BURX-ray1.80B1-241[»]
2BUTX-ray1.85B1-241[»]
2BUUX-ray1.80B1-241[»]
2BUVX-ray1.80B1-241[»]
2BUWX-ray1.80B1-241[»]
2BUXX-ray1.80B1-241[»]
2BUYX-ray1.80B1-241[»]
2BUZX-ray1.80B1-241[»]
2BV0X-ray1.80B1-241[»]
ProteinModelPortaliP20372
SMRiP20372
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20372

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105EU7 Bacteria
COG3485 LUCA
HOGENOMiHOG000174837
KOiK00449
OMAiYRWDIVL
OrthoDBiPOG091H0DHL

Family and domain databases

CDDicd03464 3_4-PCD_beta, 1 hit
Gene3Di2.60.130.10, 1 hit
InterProiView protein in InterPro
IPR000627 Intradiol_dOase_C
IPR015889 Intradiol_dOase_core
IPR024756 PCDO_beta_N
IPR012785 Protocat_dOase_b
PfamiView protein in Pfam
PF00775 Dioxygenase_C, 1 hit
PF12391 PCDO_beta_N, 1 hit
SUPFAMiSSF49482 SSF49482, 1 hit
TIGRFAMsiTIGR02422 protocat_beta, 1 hit
PROSITEiView protein in PROSITE
PS00083 INTRADIOL_DIOXYGENAS, 1 hit

Sequencei

Sequence statusi: Complete.

P20372-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQIIWGAYA QRNTEDHPPA YAPGYKTSVL RSPKNALISI AETLSEVTAP
60 70 80 90 100
HFSADKFGPK DNDLILNYAK DGLPIGERVI VHGYVRDQFG RPVKNALVEV
110 120 130 140 150
WQANASGRYR HPNDQYIGAM DPNFGGCGRM LTDDNGYYVF RTIKPGPYPW
160 170 180 190 200
RNRINEWRPA HIHFSLIADG WAQRLISQFY FEGDTLIDSC PILKTIPSEQ
210 220 230 240
QRRALIALED KSNFIEADSR CYRFDITLRG RRATYFENDL T
Length:241
Mass (Da):27,548
Last modified:July 15, 1999 - v2
Checksum:i0D2F945C92FE675A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05770 Genomic DNA Translation: AAC37153.1
CR543861 Genomic DNA Translation: CAG68553.1
PIRiC35119
RefSeqiWP_004926639.1, NC_005966.1

Genome annotation databases

EnsemblBacteriaiCAG68553; CAG68553; ACIAD1711
KEGGiaci:ACIAD1711

Similar proteinsi

Entry informationi

Entry nameiPCXB_ACIAD
AccessioniPrimary (citable) accession number: P20372
Secondary accession number(s): Q43976
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: July 15, 1999
Last modified: February 28, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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