P20371 (PCXA_ACIAD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protocatechuate 3,4-dioxygenase alpha chain
EC=1.13.11.3

Alternative name(s):
3,4-PCD

Gene names

Name:	pcaG
Ordered Locus Names:	ACIAD1712

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway.
Catalytic activity	3,4-dihydroxybenzoate + O2 = 3-carboxy-cis,cis-muconate.
Cofactor	Binds Fe3+ ion per subunit.
Pathway	Aromatic compound metabolism; beta-ketoadipate pathway; 3-carboxy-cis,cis-muconate from 3,4-dihydroxybenzoate: step 1/1.
Subunit structure	The enzyme is an oligomer of 12 copies of the alpha and beta chains.
Sequence similarities	Belongs to the intradiol ring-cleavage dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron
Molecular function	Dioxygenase Oxidoreductase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	beta-ketoadipate pathway Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ferric iron binding Inferred from electronic annotation. Source: InterPro protocatechuate 3,4-dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
pcaH	P20372	5	EBI-1029428,EBI-1029420

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 209

209

Protocatechuate 3,4-dioxygenase alpha chain

PRO_0000085094

Sites

Binding site

142

1

Protocatechuate Potential

Experimental info

Sequence conflict

64

1

G → S in AAC37154. Ref.1

Secondary structure

1

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209

Helix Strand Turn

Details...

Helix

21 – 24

4

Helix

27 – 30

4

Beta strand

52 – 57

6

Beta strand

71 – 75

5

Beta strand

101 – 105

5

Turn

108 – 110

3

Beta strand

113 – 118

6

Helix

126 – 128

3

Beta strand

135 – 140

6

Beta strand

148 – 154

7

Helix

155 – 157

3

Helix

158 – 161

4

Helix

165 – 168

4

Helix

173 – 176

4

Helix

177 – 179

3

Beta strand

180 – 186

7

Beta strand

189 – 193

5

Sequences

	Sequence		Length	Mass (Da)
	P20371 [UniParc]. Last modified August 31, 2004. Version 3. Checksum: 68FC09910CDB86EF	FASTA	209	23,484
	10 20 30 40 50 60 MNGWNFQELK ETPSQTGGPY VHIGLLPKQA NIEVFEHNLD NNLVQDNTQG QRIRLEGQVF 70 80 90 100 110 120 DGLGLPLRDV LIEIWQADTN GVYPSQADTQ GKQVDPNFLG WGRTGADFGT GFWSFNTIKP 130 140 150 160 170 180 GAVPGRKGST QAPHISLIIF ARGINIGLHT RVYFDDEAEA NAKDPVLNSI EWATRRQTLV 190 200 AKREERDGEV VYRFDIRIQG ENETVFFDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequences of genes encoding Acinetobacter calcoaceticus protocatechuate 3,4-dioxygenase: evidence indicating shuffling of genes and of DNA sequences within genes during their evolutionary divergence." Hartnett C., Neidle E.L., Ngai K.-L., Ornston L.N. J. Bacteriol. 172:956-966(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
[2]	"Contrasting patterns of evolutionary divergence within the Acinetobacter calcoaceticus pca operon." Kowalchuk G.A., Hartnett G.B., Benson A., Houghton J.E., Ngai K.-L., Ornston L.N. Gene 146:23-30(1994) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 40.
[3]	"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ADP1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L05770 Genomic DNA. Translation: AAC37154.1.
CR543861 Genomic DNA. Translation: CAG68554.1.

RefSeq

YP_046376.1. NC_005966.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EO2	X-ray	2.25	A	1-209	[»]
1EO9	X-ray	2.00	A	1-209	[»]
1EOA	X-ray	2.15	A	1-209	[»]
1EOB	X-ray	2.20	A	1-209	[»]
1EOC	X-ray	2.25	A	1-209	[»]
2BUM	X-ray	1.80	A	1-209	[»]
2BUQ	X-ray	1.80	A	1-209	[»]
2BUR	X-ray	1.80	A	1-209	[»]
2BUT	X-ray	1.85	A	1-209	[»]
2BUU	X-ray	1.80	A	1-209	[»]
2BUV	X-ray	1.80	A	1-209	[»]
2BUW	X-ray	1.80	A	1-209	[»]
2BUX	X-ray	1.80	A	1-209	[»]
2BUY	X-ray	1.80	A	1-209	[»]
2BUZ	X-ray	1.80	A	1-209	[»]
2BV0	X-ray	1.80	A	1-209	[»]

ProteinModelPortal

P20371.

SMR

P20371. Positions 8-209.

ModBase

Search...

Protein-protein interaction databases

IntAct

P20371. 1 interaction.

STRING

62977.ACIAD1712.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

CAG68554; CAG68554; ACIAD1712.

GeneID

2879436.

KEGG

aci:ACIAD1712.

PATRIC

20741194. VBIAciSp98416_1543.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG3485.

HOGENOM

HOG000174838.

KO

K00448.

OMA

RGINIHL.

ProtClustDB

CLSK2307424.

Enzyme and pathway databases

BioCyc

ASP62977:GJVV-1624-MONOMER.

UniPathway

UPA00157; UER00264.

Family and domain databases

Gene3D

2.60.130.10. 1 hit.

InterPro

IPR000627. Intradiol_dOase_C.
IPR015889. Intradiol_dOase_core.
IPR012786. Protocat_dOase_a.
[Graphical view]

Pfam

PF00775. Dioxygenase_C. 1 hit.
[Graphical view]

SUPFAM

SSF49482. Dioxygenase. 1 hit.

TIGRFAMs

TIGR02423. protocat_alph. 1 hit.

PROSITE

PS00083. INTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P20371.

Entry information

Entry name

PCXA_ACIAD

Accession

Primary (citable) accession number: P20371
Secondary accession number(s): Q43977, Q6FBK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	August 31, 2004
Last modified:	May 1, 2013
This is version 103 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program