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P20371 (PCXA_ACIAD) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protocatechuate 3,4-dioxygenase alpha chain

EC=1.13.11.3
Alternative name(s):
3,4-PCD
Gene names
Name:pcaG
Ordered Locus Names:ACIAD1712
OrganismAcinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) [Complete proteome] [HAMAP]
Taxonomic identifier62977 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter

Protein attributes

Sequence length209 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway.

Catalytic activity

3,4-dihydroxybenzoate + O2 = 3-carboxy-cis,cis-muconate.

Cofactor

Binds Fe3+ ion per subunit.

Pathway

Aromatic compound metabolism; beta-ketoadipate pathway; 3-carboxy-cis,cis-muconate from 3,4-dihydroxybenzoate: step 1/1.

Subunit structure

The enzyme is an oligomer of 12 copies of the alpha and beta chains.

Sequence similarities

Belongs to the intradiol ring-cleavage dioxygenase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

pcaHP203725EBI-1029428,EBI-1029420

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 209209Protocatechuate 3,4-dioxygenase alpha chain
PRO_0000085094

Sites

Binding site1421Protocatechuate Potential

Experimental info

Sequence conflict641G → S in AAC37154. Ref.1

Secondary structure

............................... 209
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P20371 [UniParc].

Last modified August 31, 2004. Version 3.
Checksum: 68FC09910CDB86EF

FASTA20923,484
        10         20         30         40         50         60 
MNGWNFQELK ETPSQTGGPY VHIGLLPKQA NIEVFEHNLD NNLVQDNTQG QRIRLEGQVF 

        70         80         90        100        110        120 
DGLGLPLRDV LIEIWQADTN GVYPSQADTQ GKQVDPNFLG WGRTGADFGT GFWSFNTIKP 

       130        140        150        160        170        180 
GAVPGRKGST QAPHISLIIF ARGINIGLHT RVYFDDEAEA NAKDPVLNSI EWATRRQTLV 

       190        200 
AKREERDGEV VYRFDIRIQG ENETVFFDI 

« Hide

References

« Hide 'large scale' references
[1]"DNA sequences of genes encoding Acinetobacter calcoaceticus protocatechuate 3,4-dioxygenase: evidence indicating shuffling of genes and of DNA sequences within genes during their evolutionary divergence."
Hartnett C., Neidle E.L., Ngai K.-L., Ornston L.N.
J. Bacteriol. 172:956-966(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
[2]"Contrasting patterns of evolutionary divergence within the Acinetobacter calcoaceticus pca operon."
Kowalchuk G.A., Hartnett G.B., Benson A., Houghton J.E., Ngai K.-L., Ornston L.N.
Gene 146:23-30(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 40.
[3]"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium."
Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C.
Nucleic Acids Res. 32:5766-5779(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33305 / BD413 / ADP1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L05770 Genomic DNA. Translation: AAC37154.1.
CR543861 Genomic DNA. Translation: CAG68554.1.
RefSeqYP_046376.1. NC_005966.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EO2X-ray2.25A1-209[»]
1EO9X-ray2.00A1-209[»]
1EOAX-ray2.15A1-209[»]
1EOBX-ray2.20A1-209[»]
1EOCX-ray2.25A1-209[»]
2BUMX-ray1.80A1-209[»]
2BUQX-ray1.80A1-209[»]
2BURX-ray1.80A1-209[»]
2BUTX-ray1.85A1-209[»]
2BUUX-ray1.80A1-209[»]
2BUVX-ray1.80A1-209[»]
2BUWX-ray1.80A1-209[»]
2BUXX-ray1.80A1-209[»]
2BUYX-ray1.80A1-209[»]
2BUZX-ray1.80A1-209[»]
2BV0X-ray1.80A1-209[»]
ProteinModelPortalP20371.
SMRP20371. Positions 8-209.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP20371. 1 interaction.
MINTMINT-1692742.
STRING62977.ACIAD1712.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAG68554; CAG68554; ACIAD1712.
GeneID2879436.
KEGGaci:ACIAD1712.
PATRIC20741194. VBIAciSp98416_1543.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3485.
HOGENOMHOG000174838.
KOK00448.
OMAGINIGLH.
OrthoDBEOG60PHG0.

Enzyme and pathway databases

BioCycASP62977:GJVV-1624-MONOMER.
UniPathwayUPA00157; UER00264.

Family and domain databases

Gene3D2.60.130.10. 1 hit.
InterProIPR000627. Intradiol_dOase_C.
IPR015889. Intradiol_dOase_core.
IPR012786. Protocat_dOase_a.
[Graphical view]
PfamPF00775. Dioxygenase_C. 1 hit.
[Graphical view]
SUPFAMSSF49482. SSF49482. 1 hit.
TIGRFAMsTIGR02423. protocat_alph. 1 hit.
PROSITEPS00083. INTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP20371.

Entry information

Entry namePCXA_ACIAD
AccessionPrimary (citable) accession number: P20371
Secondary accession number(s): Q43977, Q6FBK8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: August 31, 2004
Last modified: June 11, 2014
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways