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P20371

- PCXA_ACIAD

UniProt

P20371 - PCXA_ACIAD

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Protein

Protocatechuate 3,4-dioxygenase alpha chain

Gene

pcaG

Organism
Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway.

Catalytic activityi

3,4-dihydroxybenzoate + O2 = 3-carboxy-cis,cis-muconate.

Cofactori

Binds Fe3+ ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei142 – 1421ProtocatechuateSequence Analysis

GO - Molecular functioni

  1. ferric iron binding Source: InterPro
  2. protocatechuate 3,4-dioxygenase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-ketoadipate pathway Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciASP62977:GJVV-1624-MONOMER.
UniPathwayiUPA00157; UER00264.

Names & Taxonomyi

Protein namesi
Recommended name:
Protocatechuate 3,4-dioxygenase alpha chain (EC:1.13.11.3)
Alternative name(s):
3,4-PCD
Gene namesi
Name:pcaG
Ordered Locus Names:ACIAD1712
OrganismiAcinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Taxonomic identifieri62977 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter
ProteomesiUP000000430: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 209209Protocatechuate 3,4-dioxygenase alpha chainPRO_0000085094Add
BLAST

Interactioni

Subunit structurei

The enzyme is an oligomer of 12 copies of the alpha and beta chains.

Binary interactionsi

WithEntry#Exp.IntActNotes
pcaHP203725EBI-1029428,EBI-1029420

Protein-protein interaction databases

IntActiP20371. 1 interaction.
MINTiMINT-1692742.
STRINGi62977.ACIAD1712.

Structurei

Secondary structure

1
209
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi21 – 244
Helixi27 – 304
Beta strandi52 – 576
Beta strandi71 – 755
Beta strandi101 – 1055
Turni108 – 1103
Beta strandi113 – 1186
Helixi126 – 1283
Beta strandi135 – 1406
Beta strandi148 – 1547
Helixi155 – 1573
Helixi158 – 1614
Helixi165 – 1684
Helixi173 – 1764
Helixi177 – 1793
Beta strandi180 – 1867
Beta strandi189 – 1935

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EO2X-ray2.25A1-209[»]
1EO9X-ray2.00A1-209[»]
1EOAX-ray2.15A1-209[»]
1EOBX-ray2.20A1-209[»]
1EOCX-ray2.25A1-209[»]
2BUMX-ray1.80A1-209[»]
2BUQX-ray1.80A1-209[»]
2BURX-ray1.80A1-209[»]
2BUTX-ray1.85A1-209[»]
2BUUX-ray1.80A1-209[»]
2BUVX-ray1.80A1-209[»]
2BUWX-ray1.80A1-209[»]
2BUXX-ray1.80A1-209[»]
2BUYX-ray1.80A1-209[»]
2BUZX-ray1.80A1-209[»]
2BV0X-ray1.80A1-209[»]
ProteinModelPortaliP20371.
SMRiP20371. Positions 8-209.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20371.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3485.
HOGENOMiHOG000174838.
KOiK00448.
OMAiGINIGLH.
OrthoDBiEOG60PHG0.

Family and domain databases

Gene3Di2.60.130.10. 1 hit.
InterProiIPR000627. Intradiol_dOase_C.
IPR015889. Intradiol_dOase_core.
IPR012786. Protocat_dOase_a.
[Graphical view]
PfamiPF00775. Dioxygenase_C. 1 hit.
[Graphical view]
SUPFAMiSSF49482. SSF49482. 1 hit.
TIGRFAMsiTIGR02423. protocat_alph. 1 hit.
PROSITEiPS00083. INTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20371-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNGWNFQELK ETPSQTGGPY VHIGLLPKQA NIEVFEHNLD NNLVQDNTQG
60 70 80 90 100
QRIRLEGQVF DGLGLPLRDV LIEIWQADTN GVYPSQADTQ GKQVDPNFLG
110 120 130 140 150
WGRTGADFGT GFWSFNTIKP GAVPGRKGST QAPHISLIIF ARGINIGLHT
160 170 180 190 200
RVYFDDEAEA NAKDPVLNSI EWATRRQTLV AKREERDGEV VYRFDIRIQG

ENETVFFDI
Length:209
Mass (Da):23,484
Last modified:August 31, 2004 - v3
Checksum:i68FC09910CDB86EF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 641G → S in AAC37154. (PubMed:2298704)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L05770 Genomic DNA. Translation: AAC37154.1.
CR543861 Genomic DNA. Translation: CAG68554.1.
RefSeqiYP_046376.1. NC_005966.1.

Genome annotation databases

EnsemblBacteriaiCAG68554; CAG68554; ACIAD1712.
GeneIDi2879436.
KEGGiaci:ACIAD1712.
PATRICi20741194. VBIAciSp98416_1543.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L05770 Genomic DNA. Translation: AAC37154.1 .
CR543861 Genomic DNA. Translation: CAG68554.1 .
RefSeqi YP_046376.1. NC_005966.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1EO2 X-ray 2.25 A 1-209 [» ]
1EO9 X-ray 2.00 A 1-209 [» ]
1EOA X-ray 2.15 A 1-209 [» ]
1EOB X-ray 2.20 A 1-209 [» ]
1EOC X-ray 2.25 A 1-209 [» ]
2BUM X-ray 1.80 A 1-209 [» ]
2BUQ X-ray 1.80 A 1-209 [» ]
2BUR X-ray 1.80 A 1-209 [» ]
2BUT X-ray 1.85 A 1-209 [» ]
2BUU X-ray 1.80 A 1-209 [» ]
2BUV X-ray 1.80 A 1-209 [» ]
2BUW X-ray 1.80 A 1-209 [» ]
2BUX X-ray 1.80 A 1-209 [» ]
2BUY X-ray 1.80 A 1-209 [» ]
2BUZ X-ray 1.80 A 1-209 [» ]
2BV0 X-ray 1.80 A 1-209 [» ]
ProteinModelPortali P20371.
SMRi P20371. Positions 8-209.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P20371. 1 interaction.
MINTi MINT-1692742.
STRINGi 62977.ACIAD1712.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAG68554 ; CAG68554 ; ACIAD1712 .
GeneIDi 2879436.
KEGGi aci:ACIAD1712.
PATRICi 20741194. VBIAciSp98416_1543.

Phylogenomic databases

eggNOGi COG3485.
HOGENOMi HOG000174838.
KOi K00448.
OMAi GINIGLH.
OrthoDBi EOG60PHG0.

Enzyme and pathway databases

UniPathwayi UPA00157 ; UER00264 .
BioCyci ASP62977:GJVV-1624-MONOMER.

Miscellaneous databases

EvolutionaryTracei P20371.

Family and domain databases

Gene3Di 2.60.130.10. 1 hit.
InterProi IPR000627. Intradiol_dOase_C.
IPR015889. Intradiol_dOase_core.
IPR012786. Protocat_dOase_a.
[Graphical view ]
Pfami PF00775. Dioxygenase_C. 1 hit.
[Graphical view ]
SUPFAMi SSF49482. SSF49482. 1 hit.
TIGRFAMsi TIGR02423. protocat_alph. 1 hit.
PROSITEi PS00083. INTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequences of genes encoding Acinetobacter calcoaceticus protocatechuate 3,4-dioxygenase: evidence indicating shuffling of genes and of DNA sequences within genes during their evolutionary divergence."
    Hartnett C., Neidle E.L., Ngai K.-L., Ornston L.N.
    J. Bacteriol. 172:956-966(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
  2. "Contrasting patterns of evolutionary divergence within the Acinetobacter calcoaceticus pca operon."
    Kowalchuk G.A., Hartnett G.B., Benson A., Houghton J.E., Ngai K.-L., Ornston L.N.
    Gene 146:23-30(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 40.
  3. "Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium."
    Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C.
    Nucleic Acids Res. 32:5766-5779(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33305 / BD413 / ADP1.

Entry informationi

Entry nameiPCXA_ACIAD
AccessioniPrimary (citable) accession number: P20371
Secondary accession number(s): Q43977, Q6FBK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: August 31, 2004
Last modified: October 29, 2014
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3