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P20371

- PCXA_ACIAD

UniProt

P20371 - PCXA_ACIAD

Protein

Protocatechuate 3,4-dioxygenase alpha chain

Gene

pcaG

Organism
Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 3 (31 Aug 2004)
      Previous versions | rss
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    Functioni

    Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway.

    Catalytic activityi

    3,4-dihydroxybenzoate + O2 = 3-carboxy-cis,cis-muconate.

    Cofactori

    Binds Fe3+ ion per subunit.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei142 – 1421ProtocatechuateSequence Analysis

    GO - Molecular functioni

    1. ferric iron binding Source: InterPro
    2. protein binding Source: IntAct
    3. protocatechuate 3,4-dioxygenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-ketoadipate pathway Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Biological processi

    Aromatic hydrocarbons catabolism

    Keywords - Ligandi

    Iron

    Enzyme and pathway databases

    BioCyciASP62977:GJVV-1624-MONOMER.
    UniPathwayiUPA00157; UER00264.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protocatechuate 3,4-dioxygenase alpha chain (EC:1.13.11.3)
    Alternative name(s):
    3,4-PCD
    Gene namesi
    Name:pcaG
    Ordered Locus Names:ACIAD1712
    OrganismiAcinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
    Taxonomic identifieri62977 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter
    ProteomesiUP000000430: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 209209Protocatechuate 3,4-dioxygenase alpha chainPRO_0000085094Add
    BLAST

    Interactioni

    Subunit structurei

    The enzyme is an oligomer of 12 copies of the alpha and beta chains.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    pcaHP203725EBI-1029428,EBI-1029420

    Protein-protein interaction databases

    IntActiP20371. 1 interaction.
    MINTiMINT-1692742.
    STRINGi62977.ACIAD1712.

    Structurei

    Secondary structure

    1
    209
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi21 – 244
    Helixi27 – 304
    Beta strandi52 – 576
    Beta strandi71 – 755
    Beta strandi101 – 1055
    Turni108 – 1103
    Beta strandi113 – 1186
    Helixi126 – 1283
    Beta strandi135 – 1406
    Beta strandi148 – 1547
    Helixi155 – 1573
    Helixi158 – 1614
    Helixi165 – 1684
    Helixi173 – 1764
    Helixi177 – 1793
    Beta strandi180 – 1867
    Beta strandi189 – 1935

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1EO2X-ray2.25A1-209[»]
    1EO9X-ray2.00A1-209[»]
    1EOAX-ray2.15A1-209[»]
    1EOBX-ray2.20A1-209[»]
    1EOCX-ray2.25A1-209[»]
    2BUMX-ray1.80A1-209[»]
    2BUQX-ray1.80A1-209[»]
    2BURX-ray1.80A1-209[»]
    2BUTX-ray1.85A1-209[»]
    2BUUX-ray1.80A1-209[»]
    2BUVX-ray1.80A1-209[»]
    2BUWX-ray1.80A1-209[»]
    2BUXX-ray1.80A1-209[»]
    2BUYX-ray1.80A1-209[»]
    2BUZX-ray1.80A1-209[»]
    2BV0X-ray1.80A1-209[»]
    ProteinModelPortaliP20371.
    SMRiP20371. Positions 8-209.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP20371.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG3485.
    HOGENOMiHOG000174838.
    KOiK00448.
    OMAiGINIGLH.
    OrthoDBiEOG60PHG0.

    Family and domain databases

    Gene3Di2.60.130.10. 1 hit.
    InterProiIPR000627. Intradiol_dOase_C.
    IPR015889. Intradiol_dOase_core.
    IPR012786. Protocat_dOase_a.
    [Graphical view]
    PfamiPF00775. Dioxygenase_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF49482. SSF49482. 1 hit.
    TIGRFAMsiTIGR02423. protocat_alph. 1 hit.
    PROSITEiPS00083. INTRADIOL_DIOXYGENAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P20371-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNGWNFQELK ETPSQTGGPY VHIGLLPKQA NIEVFEHNLD NNLVQDNTQG    50
    QRIRLEGQVF DGLGLPLRDV LIEIWQADTN GVYPSQADTQ GKQVDPNFLG 100
    WGRTGADFGT GFWSFNTIKP GAVPGRKGST QAPHISLIIF ARGINIGLHT 150
    RVYFDDEAEA NAKDPVLNSI EWATRRQTLV AKREERDGEV VYRFDIRIQG 200
    ENETVFFDI 209
    Length:209
    Mass (Da):23,484
    Last modified:August 31, 2004 - v3
    Checksum:i68FC09910CDB86EF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti64 – 641G → S in AAC37154. (PubMed:2298704)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L05770 Genomic DNA. Translation: AAC37154.1.
    CR543861 Genomic DNA. Translation: CAG68554.1.
    RefSeqiYP_046376.1. NC_005966.1.

    Genome annotation databases

    EnsemblBacteriaiCAG68554; CAG68554; ACIAD1712.
    GeneIDi2879436.
    KEGGiaci:ACIAD1712.
    PATRICi20741194. VBIAciSp98416_1543.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L05770 Genomic DNA. Translation: AAC37154.1 .
    CR543861 Genomic DNA. Translation: CAG68554.1 .
    RefSeqi YP_046376.1. NC_005966.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1EO2 X-ray 2.25 A 1-209 [» ]
    1EO9 X-ray 2.00 A 1-209 [» ]
    1EOA X-ray 2.15 A 1-209 [» ]
    1EOB X-ray 2.20 A 1-209 [» ]
    1EOC X-ray 2.25 A 1-209 [» ]
    2BUM X-ray 1.80 A 1-209 [» ]
    2BUQ X-ray 1.80 A 1-209 [» ]
    2BUR X-ray 1.80 A 1-209 [» ]
    2BUT X-ray 1.85 A 1-209 [» ]
    2BUU X-ray 1.80 A 1-209 [» ]
    2BUV X-ray 1.80 A 1-209 [» ]
    2BUW X-ray 1.80 A 1-209 [» ]
    2BUX X-ray 1.80 A 1-209 [» ]
    2BUY X-ray 1.80 A 1-209 [» ]
    2BUZ X-ray 1.80 A 1-209 [» ]
    2BV0 X-ray 1.80 A 1-209 [» ]
    ProteinModelPortali P20371.
    SMRi P20371. Positions 8-209.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P20371. 1 interaction.
    MINTi MINT-1692742.
    STRINGi 62977.ACIAD1712.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAG68554 ; CAG68554 ; ACIAD1712 .
    GeneIDi 2879436.
    KEGGi aci:ACIAD1712.
    PATRICi 20741194. VBIAciSp98416_1543.

    Phylogenomic databases

    eggNOGi COG3485.
    HOGENOMi HOG000174838.
    KOi K00448.
    OMAi GINIGLH.
    OrthoDBi EOG60PHG0.

    Enzyme and pathway databases

    UniPathwayi UPA00157 ; UER00264 .
    BioCyci ASP62977:GJVV-1624-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P20371.

    Family and domain databases

    Gene3Di 2.60.130.10. 1 hit.
    InterProi IPR000627. Intradiol_dOase_C.
    IPR015889. Intradiol_dOase_core.
    IPR012786. Protocat_dOase_a.
    [Graphical view ]
    Pfami PF00775. Dioxygenase_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49482. SSF49482. 1 hit.
    TIGRFAMsi TIGR02423. protocat_alph. 1 hit.
    PROSITEi PS00083. INTRADIOL_DIOXYGENAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequences of genes encoding Acinetobacter calcoaceticus protocatechuate 3,4-dioxygenase: evidence indicating shuffling of genes and of DNA sequences within genes during their evolutionary divergence."
      Hartnett C., Neidle E.L., Ngai K.-L., Ornston L.N.
      J. Bacteriol. 172:956-966(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
    2. "Contrasting patterns of evolutionary divergence within the Acinetobacter calcoaceticus pca operon."
      Kowalchuk G.A., Hartnett G.B., Benson A., Houghton J.E., Ngai K.-L., Ornston L.N.
      Gene 146:23-30(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION TO 40.
    3. "Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium."
      Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C.
      Nucleic Acids Res. 32:5766-5779(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33305 / BD413 / ADP1.

    Entry informationi

    Entry nameiPCXA_ACIAD
    AccessioniPrimary (citable) accession number: P20371
    Secondary accession number(s): Q43977, Q6FBK8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: August 31, 2004
    Last modified: October 1, 2014
    This is version 110 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3