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Protein

Protocatechuate 3,4-dioxygenase alpha chain

Gene

pcaG

Organism
Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway.

Catalytic activityi

3,4-dihydroxybenzoate + O2 = 3-carboxy-cis,cis-muconate.

Cofactori

Fe3+Note: Binds Fe3+ ion per subunit.

Pathwayi: beta-ketoadipate pathway

This protein is involved in step 1 of the subpathway that synthesizes 3-carboxy-cis,cis-muconate from 3,4-dihydroxybenzoate.
Proteins known to be involved in this subpathway in this organism are:
  1. Protocatechuate 3,4-dioxygenase alpha chain (pcaG), Protocatechuate 3,4-dioxygenase beta chain (pcaH)
This subpathway is part of the pathway beta-ketoadipate pathway, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3-carboxy-cis,cis-muconate from 3,4-dihydroxybenzoate, the pathway beta-ketoadipate pathway and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei142ProtocatechuateSequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Iron

Enzyme and pathway databases

UniPathwayiUPA00157; UER00264.

Names & Taxonomyi

Protein namesi
Recommended name:
Protocatechuate 3,4-dioxygenase alpha chain (EC:1.13.11.3)
Alternative name(s):
3,4-PCD
Gene namesi
Name:pcaG
Ordered Locus Names:ACIAD1712
OrganismiAcinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Taxonomic identifieri62977 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter
Proteomesi
  • UP000000430 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000850941 – 209Protocatechuate 3,4-dioxygenase alpha chainAdd BLAST209

Interactioni

Subunit structurei

The enzyme is an oligomer of 12 copies of the alpha and beta chains.

Binary interactionsi

WithEntry#Exp.IntActNotes
pcaHP203725EBI-1029428,EBI-1029420

Protein-protein interaction databases

IntActiP20371. 1 interactor.
MINTiMINT-1692742.
STRINGi62977.ACIAD1712.

Structurei

Secondary structure

1209
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi21 – 24Combined sources4
Helixi27 – 30Combined sources4
Beta strandi52 – 57Combined sources6
Beta strandi71 – 75Combined sources5
Beta strandi101 – 105Combined sources5
Turni108 – 110Combined sources3
Beta strandi113 – 118Combined sources6
Helixi126 – 128Combined sources3
Beta strandi135 – 140Combined sources6
Beta strandi148 – 154Combined sources7
Helixi155 – 157Combined sources3
Helixi158 – 161Combined sources4
Helixi165 – 168Combined sources4
Helixi173 – 176Combined sources4
Helixi177 – 179Combined sources3
Beta strandi180 – 186Combined sources7
Beta strandi189 – 193Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EO2X-ray2.25A1-209[»]
1EO9X-ray2.00A1-209[»]
1EOAX-ray2.15A1-209[»]
1EOBX-ray2.20A1-209[»]
1EOCX-ray2.25A1-209[»]
2BUMX-ray1.80A1-209[»]
2BUQX-ray1.80A1-209[»]
2BURX-ray1.80A1-209[»]
2BUTX-ray1.85A1-209[»]
2BUUX-ray1.80A1-209[»]
2BUVX-ray1.80A1-209[»]
2BUWX-ray1.80A1-209[»]
2BUXX-ray1.80A1-209[»]
2BUYX-ray1.80A1-209[»]
2BUZX-ray1.80A1-209[»]
2BV0X-ray1.80A1-209[»]
ProteinModelPortaliP20371.
SMRiP20371.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20371.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105RM9. Bacteria.
COG3485. LUCA.
HOGENOMiHOG000174838.
KOiK00448.
OMAiGINIGLH.
OrthoDBiPOG091H0DHL.

Family and domain databases

CDDicd03463. 3_4-PCD_alpha. 1 hit.
Gene3Di2.60.130.10. 1 hit.
InterProiIPR000627. Intradiol_dOase_C.
IPR015889. Intradiol_dOase_core.
IPR012786. Protocat_dOase_a.
[Graphical view]
PfamiPF00775. Dioxygenase_C. 1 hit.
[Graphical view]
SUPFAMiSSF49482. SSF49482. 1 hit.
TIGRFAMsiTIGR02423. protocat_alph. 1 hit.
PROSITEiPS00083. INTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20371-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGWNFQELK ETPSQTGGPY VHIGLLPKQA NIEVFEHNLD NNLVQDNTQG
60 70 80 90 100
QRIRLEGQVF DGLGLPLRDV LIEIWQADTN GVYPSQADTQ GKQVDPNFLG
110 120 130 140 150
WGRTGADFGT GFWSFNTIKP GAVPGRKGST QAPHISLIIF ARGINIGLHT
160 170 180 190 200
RVYFDDEAEA NAKDPVLNSI EWATRRQTLV AKREERDGEV VYRFDIRIQG

ENETVFFDI
Length:209
Mass (Da):23,484
Last modified:August 31, 2004 - v3
Checksum:i68FC09910CDB86EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64G → S in AAC37154 (PubMed:2298704).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05770 Genomic DNA. Translation: AAC37154.1.
CR543861 Genomic DNA. Translation: CAG68554.1.
RefSeqiWP_004926643.1. NC_005966.1.

Genome annotation databases

EnsemblBacteriaiCAG68554; CAG68554; ACIAD1712.
GeneIDi25684314.
KEGGiaci:ACIAD1712.
PATRICi20741194. VBIAciSp98416_1543.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05770 Genomic DNA. Translation: AAC37154.1.
CR543861 Genomic DNA. Translation: CAG68554.1.
RefSeqiWP_004926643.1. NC_005966.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EO2X-ray2.25A1-209[»]
1EO9X-ray2.00A1-209[»]
1EOAX-ray2.15A1-209[»]
1EOBX-ray2.20A1-209[»]
1EOCX-ray2.25A1-209[»]
2BUMX-ray1.80A1-209[»]
2BUQX-ray1.80A1-209[»]
2BURX-ray1.80A1-209[»]
2BUTX-ray1.85A1-209[»]
2BUUX-ray1.80A1-209[»]
2BUVX-ray1.80A1-209[»]
2BUWX-ray1.80A1-209[»]
2BUXX-ray1.80A1-209[»]
2BUYX-ray1.80A1-209[»]
2BUZX-ray1.80A1-209[»]
2BV0X-ray1.80A1-209[»]
ProteinModelPortaliP20371.
SMRiP20371.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP20371. 1 interactor.
MINTiMINT-1692742.
STRINGi62977.ACIAD1712.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG68554; CAG68554; ACIAD1712.
GeneIDi25684314.
KEGGiaci:ACIAD1712.
PATRICi20741194. VBIAciSp98416_1543.

Phylogenomic databases

eggNOGiENOG4105RM9. Bacteria.
COG3485. LUCA.
HOGENOMiHOG000174838.
KOiK00448.
OMAiGINIGLH.
OrthoDBiPOG091H0DHL.

Enzyme and pathway databases

UniPathwayiUPA00157; UER00264.

Miscellaneous databases

EvolutionaryTraceiP20371.
PROiP20371.

Family and domain databases

CDDicd03463. 3_4-PCD_alpha. 1 hit.
Gene3Di2.60.130.10. 1 hit.
InterProiIPR000627. Intradiol_dOase_C.
IPR015889. Intradiol_dOase_core.
IPR012786. Protocat_dOase_a.
[Graphical view]
PfamiPF00775. Dioxygenase_C. 1 hit.
[Graphical view]
SUPFAMiSSF49482. SSF49482. 1 hit.
TIGRFAMsiTIGR02423. protocat_alph. 1 hit.
PROSITEiPS00083. INTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCXA_ACIAD
AccessioniPrimary (citable) accession number: P20371
Secondary accession number(s): Q43977, Q6FBK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: August 31, 2004
Last modified: November 2, 2016
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.