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Protein

Protocatechuate 3,4-dioxygenase alpha chain

Gene

pcaG

Organism
Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway.

Catalytic activityi

3,4-dihydroxybenzoate + O2 = 3-carboxy-cis,cis-muconate.

Cofactori

Fe3+Note: Binds Fe3+ ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei142 – 1421ProtocatechuateSequence Analysis

GO - Molecular functioni

  1. ferric iron binding Source: InterPro
  2. protocatechuate 3,4-dioxygenase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-ketoadipate pathway Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciASP62977:GJVV-1624-MONOMER.
UniPathwayiUPA00157; UER00264.

Names & Taxonomyi

Protein namesi
Recommended name:
Protocatechuate 3,4-dioxygenase alpha chain (EC:1.13.11.3)
Alternative name(s):
3,4-PCD
Gene namesi
Name:pcaG
Ordered Locus Names:ACIAD1712
OrganismiAcinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Taxonomic identifieri62977 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter
ProteomesiUP000000430: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 209209Protocatechuate 3,4-dioxygenase alpha chainPRO_0000085094Add
BLAST

Interactioni

Subunit structurei

The enzyme is an oligomer of 12 copies of the alpha and beta chains.

Binary interactionsi

WithEntry#Exp.IntActNotes
pcaHP203725EBI-1029428,EBI-1029420

Protein-protein interaction databases

IntActiP20371. 1 interaction.
MINTiMINT-1692742.
STRINGi62977.ACIAD1712.

Structurei

Secondary structure

1
209
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi21 – 244Combined sources
Helixi27 – 304Combined sources
Beta strandi52 – 576Combined sources
Beta strandi71 – 755Combined sources
Beta strandi101 – 1055Combined sources
Turni108 – 1103Combined sources
Beta strandi113 – 1186Combined sources
Helixi126 – 1283Combined sources
Beta strandi135 – 1406Combined sources
Beta strandi148 – 1547Combined sources
Helixi155 – 1573Combined sources
Helixi158 – 1614Combined sources
Helixi165 – 1684Combined sources
Helixi173 – 1764Combined sources
Helixi177 – 1793Combined sources
Beta strandi180 – 1867Combined sources
Beta strandi189 – 1935Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EO2X-ray2.25A1-209[»]
1EO9X-ray2.00A1-209[»]
1EOAX-ray2.15A1-209[»]
1EOBX-ray2.20A1-209[»]
1EOCX-ray2.25A1-209[»]
2BUMX-ray1.80A1-209[»]
2BUQX-ray1.80A1-209[»]
2BURX-ray1.80A1-209[»]
2BUTX-ray1.85A1-209[»]
2BUUX-ray1.80A1-209[»]
2BUVX-ray1.80A1-209[»]
2BUWX-ray1.80A1-209[»]
2BUXX-ray1.80A1-209[»]
2BUYX-ray1.80A1-209[»]
2BUZX-ray1.80A1-209[»]
2BV0X-ray1.80A1-209[»]
ProteinModelPortaliP20371.
SMRiP20371. Positions 8-209.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20371.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3485.
HOGENOMiHOG000174838.
KOiK00448.
OMAiTRMYFPE.
OrthoDBiEOG60PHG0.

Family and domain databases

Gene3Di2.60.130.10. 1 hit.
InterProiIPR000627. Intradiol_dOase_C.
IPR015889. Intradiol_dOase_core.
IPR012786. Protocat_dOase_a.
[Graphical view]
PfamiPF00775. Dioxygenase_C. 1 hit.
[Graphical view]
SUPFAMiSSF49482. SSF49482. 1 hit.
TIGRFAMsiTIGR02423. protocat_alph. 1 hit.
PROSITEiPS00083. INTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20371-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGWNFQELK ETPSQTGGPY VHIGLLPKQA NIEVFEHNLD NNLVQDNTQG
60 70 80 90 100
QRIRLEGQVF DGLGLPLRDV LIEIWQADTN GVYPSQADTQ GKQVDPNFLG
110 120 130 140 150
WGRTGADFGT GFWSFNTIKP GAVPGRKGST QAPHISLIIF ARGINIGLHT
160 170 180 190 200
RVYFDDEAEA NAKDPVLNSI EWATRRQTLV AKREERDGEV VYRFDIRIQG

ENETVFFDI
Length:209
Mass (Da):23,484
Last modified:August 31, 2004 - v3
Checksum:i68FC09910CDB86EF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 641G → S in AAC37154 (PubMed:2298704).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05770 Genomic DNA. Translation: AAC37154.1.
CR543861 Genomic DNA. Translation: CAG68554.1.
RefSeqiYP_046376.1. NC_005966.1.

Genome annotation databases

EnsemblBacteriaiCAG68554; CAG68554; ACIAD1712.
GeneIDi2879436.
KEGGiaci:ACIAD1712.
PATRICi20741194. VBIAciSp98416_1543.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05770 Genomic DNA. Translation: AAC37154.1.
CR543861 Genomic DNA. Translation: CAG68554.1.
RefSeqiYP_046376.1. NC_005966.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EO2X-ray2.25A1-209[»]
1EO9X-ray2.00A1-209[»]
1EOAX-ray2.15A1-209[»]
1EOBX-ray2.20A1-209[»]
1EOCX-ray2.25A1-209[»]
2BUMX-ray1.80A1-209[»]
2BUQX-ray1.80A1-209[»]
2BURX-ray1.80A1-209[»]
2BUTX-ray1.85A1-209[»]
2BUUX-ray1.80A1-209[»]
2BUVX-ray1.80A1-209[»]
2BUWX-ray1.80A1-209[»]
2BUXX-ray1.80A1-209[»]
2BUYX-ray1.80A1-209[»]
2BUZX-ray1.80A1-209[»]
2BV0X-ray1.80A1-209[»]
ProteinModelPortaliP20371.
SMRiP20371. Positions 8-209.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP20371. 1 interaction.
MINTiMINT-1692742.
STRINGi62977.ACIAD1712.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG68554; CAG68554; ACIAD1712.
GeneIDi2879436.
KEGGiaci:ACIAD1712.
PATRICi20741194. VBIAciSp98416_1543.

Phylogenomic databases

eggNOGiCOG3485.
HOGENOMiHOG000174838.
KOiK00448.
OMAiTRMYFPE.
OrthoDBiEOG60PHG0.

Enzyme and pathway databases

UniPathwayiUPA00157; UER00264.
BioCyciASP62977:GJVV-1624-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP20371.

Family and domain databases

Gene3Di2.60.130.10. 1 hit.
InterProiIPR000627. Intradiol_dOase_C.
IPR015889. Intradiol_dOase_core.
IPR012786. Protocat_dOase_a.
[Graphical view]
PfamiPF00775. Dioxygenase_C. 1 hit.
[Graphical view]
SUPFAMiSSF49482. SSF49482. 1 hit.
TIGRFAMsiTIGR02423. protocat_alph. 1 hit.
PROSITEiPS00083. INTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequences of genes encoding Acinetobacter calcoaceticus protocatechuate 3,4-dioxygenase: evidence indicating shuffling of genes and of DNA sequences within genes during their evolutionary divergence."
    Hartnett C., Neidle E.L., Ngai K.-L., Ornston L.N.
    J. Bacteriol. 172:956-966(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
  2. "Contrasting patterns of evolutionary divergence within the Acinetobacter calcoaceticus pca operon."
    Kowalchuk G.A., Hartnett G.B., Benson A., Houghton J.E., Ngai K.-L., Ornston L.N.
    Gene 146:23-30(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 40.
  3. "Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium."
    Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C.
    Nucleic Acids Res. 32:5766-5779(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33305 / BD413 / ADP1.

Entry informationi

Entry nameiPCXA_ACIAD
AccessioniPrimary (citable) accession number: P20371
Secondary accession number(s): Q43977, Q6FBK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: August 31, 2004
Last modified: January 7, 2015
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.