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Reviewed, UniProtKB/Swiss-Prot P20368 (ADH1_ZYMMO)

Last modified June 16, 2009. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alcohol dehydrogenase 1
    EC=1.1.1.1
Alternative name(s):
    Alcohol dehydrogenase I
      Short name=ADH I
Gene names
Name: adhA
Ordered Locus Names: ZMO1236
OrganismZymomonas mobilis [Complete proteome] [HAMAP]
Taxonomic identifier542 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Zymomonas

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Protein attributes

Sequence length337 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

An alcohol + NAD+ = an aldehyde or ketone + NADH.

Cofactor

Binds 2 zinc ions per subunit By similarity.

Enzyme regulation

Inhibited by ethanol.

Pathway

Alcohol metabolism; ethanol biosynthesis via fermentation pathway.

Subunit structure

Multimeric (with different ratios of monomers).

Miscellaneous

In Z.mobilis there are two isozymes of alcohol dehydrogenase.

Sequence similarities

Belongs to the zinc-containing alcohol dehydrogenase family.

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Ontologies

Keywords
   LigandMetal-binding
NAD
Zinc
   Molecular functionOxidoreductase
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionalcohol dehydrogenase activity

Inferred from electronic annotation. Source: EC

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 337337Alcohol dehydrogenase 1
PRO_0000160750

Sites

Metal binding371Zinc 1; catalytic By similarity
Metal binding581Zinc 1; catalytic By similarity
Metal binding891Zinc 2 By similarity
Metal binding921Zinc 2 By similarity
Metal binding951Zinc 2 By similarity
Metal binding1031Zinc 2 By similarity
Metal binding1451Zinc 1; catalytic By similarity

Experimental info

Sequence conflict171T → I AA sequence Ref.5
Sequence conflict261E → F AA sequence Ref.5
Sequence conflict281L → H AA sequence Ref.5
Sequence conflict301E → P AA sequence Ref.5
Sequence conflict761V → A in AAA27682. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P20368-1 [UniParc].

Last modified February 15, 2005. Version 2.
Checksum: 98D75C912CE1EBE5

FASTA33736,122
        10         20         30         40         50         60 
MKAAVITKDH TIEVKDTKLR PLKYGEALLE MEYCGVCHTD LHVKNGDFGD ETGRITGHEG 

        70         80         90        100        110        120 
IGIVKQVGEG VTSLKVGDRA SVAWFFKGCG HCEYCVSGNE TLCRNVENAG YTVDGAMAEE 

       130        140        150        160        170        180 
CIVVADYSVK VPDGLDPAVA SSITCAGVTT YKAVKVSQIQ PGQWLAIYGL GGLGNLALQY 

       190        200        210        220        230        240 
AKNVFNAKVI AIDVNDEQLA FAKELGADMV INPKNEDAAK IIQEKVGGAH ATVVTAVAKS 

       250        260        270        280        290        300 
AFNSAVEAIR AGGRVVAVGL PPEKMDLSIP RLVLDGIEVL GSLVGTREDL KEAFQFAAEG 

       310        320        330 
KVKPKVTKRK VEEINQIFDE MEHGKFTGRM VVDFTHH

« Hide

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References

« Hide 'large scale' references
[1]"Cloning of the Zymomonas mobilis structural gene encoding alcohol dehydrogenase I (adhA): sequence comparison and expression in Escherichia coli."
Keshav K.F., Yomano L.P., An H., Ingram L.O.
J. Bacteriol. 172:2491-2497(1990) [PubMed: 2185223] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 31821 / ZM4 / CP4.
[2]"Mannitol dehydrogenase from Zymomonas mobilis."
O'Mullan P.J., Stein D., Chase T. Jr., Eveleigh D.E.
Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 31821 / ZM4 / CP4.
[3]"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4."
Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. expand/collapse author list , Kang H.L., Lee S.Y., Lee K.J., Kang H.S.
Nat. Biotechnol. 23:63-68(2005) [PubMed: 15592456] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31821 / ZM4 / CP4.
[4]"Cloning, sequencing, and expression of the Zymomonas mobilis phosphoglycerate mutase gene (pgm) in Escherichia coli."
Yomano L.P., Scopes R.K., Ingram L.O.
J. Bacteriol. 175:3926-3933(1993) [PubMed: 8320209] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40.
Strain: ATCC 31821 / ZM4 / CP4.
[5]"The two alcohol dehydrogenases of Zymomonas mobilis. Purification by differential dye ligand chromatography, molecular characterisation and physiological roles."
Neale A.D., Scopes R.K., Kelly J.M., Wettenhall R.E.H.
Eur. J. Biochem. 154:119-124(1986) [PubMed: 2935393] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-31.

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Cross-references

Sequence databases

M32100 Genomic DNA. Translation: AAA27682.1.
AY170008 Genomic DNA. Translation: AAO38758.1.
AE008692 Genomic DNA. Translation: AAV89860.1.
L09650 Genomic DNA. Translation: AAA71935.2.
PIRA35260.
RefSeqYP_162971.1.

3D structure databases

HSSPHSSP built from PDB template 1JVB based on UniProtKB P39462.
ModBaseSearch...

Genome annotation databases

GeneID3188393.
GenomeReviewsGene locus ZMO1236 in contig AE008692_GR.
KEGGzmo:ZMO1236.
NMPDRfig|264203.3.peg.428.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP20368.
OMAP20368. VRPGQWI.

Enzyme and pathway databases

BioCycZMOB264203:ZMO1236-MON.
BRENDA1.1.1.1. 1658.

Family and domain databases

InterProIPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn.
IPR013149. ADH_Zn-bd.
IPR002328. ADH_Zn_CS.
[Graphical view]
PANTHERPTHR11695. ADH_Sf_Zn. 1 hit.
PfamPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
PROSITEPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameADH1_ZYMMO
AccessionPrimary (citable) accession number: P20368
Secondary accession number(s): Q5NN50, Q6Y8J4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 15, 2005
Last modified: June 16, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents