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P20366 (TKN1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 151. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protachykinin-1
Alternative name(s):
PPT

Cleaved into the following 5 chains:

  1. Substance P
  2. Neurokinin A
    Short name=NKA
    Alternative name(s):
    Neuromedin L
    Substance K
  3. Neuropeptide K
    Short name=NPK
  4. Neuropeptide gamma
  5. C-terminal-flanking peptide
Gene names
Name:TAC1
Synonyms:NKA, NKNA, TAC2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length129 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.

Subcellular location

Secreted.

Sequence similarities

Belongs to the tachykinin family.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionNeuropeptide
Neurotransmitter
   PTMAmidation
Cleavage on pair of basic residues
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processassociative learning

Inferred from electronic annotation. Source: Ensembl

cell-cell signaling

Traceable author statement Ref.9. Source: ProtInc

detection of abiotic stimulus

Traceable author statement PubMed 9482938. Source: ProtInc

inflammatory response

Inferred from electronic annotation. Source: Ensembl

insemination

Traceable author statement Ref.9. Source: ProtInc

long-term memory

Inferred from electronic annotation. Source: Ensembl

negative regulation of heart rate

Inferred from electronic annotation. Source: Ensembl

neuropeptide signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of action potential

Inferred from electronic annotation. Source: Ensembl

positive regulation of acute inflammatory response

Inferred from electronic annotation. Source: Ensembl

positive regulation of corticosterone secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of cytosolic calcium ion concentration

Inferred from direct assay PubMed 8957234. Source: UniProtKB

positive regulation of epithelial cell migration

Inferred from electronic annotation. Source: Ensembl

positive regulation of lymphocyte proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of ossification

Inferred from electronic annotation. Source: Ensembl

positive regulation of renal sodium excretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of saliva secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of stress fiber assembly

Inferred from electronic annotation. Source: Ensembl

positive regulation of synaptic transmission, GABAergic

Inferred from electronic annotation. Source: Ensembl

positive regulation of synaptic transmission, cholinergic

Inferred from electronic annotation. Source: Ensembl

regulation of blood pressure

Inferred from electronic annotation. Source: Ensembl

response to hormone

Inferred from electronic annotation. Source: Ensembl

response to lipopolysaccharide

Inferred from electronic annotation. Source: Ensembl

response to morphine

Inferred from electronic annotation. Source: Ensembl

response to pain

Inferred from electronic annotation. Source: Ensembl

sensory perception of pain

Inferred from electronic annotation. Source: Ensembl

synaptic transmission

Inferred from electronic annotation. Source: UniProtKB-KW

tachykinin receptor signaling pathway

Inferred from electronic annotation. Source: InterPro

   Cellular_componentaxon

Inferred from electronic annotation. Source: Ensembl

extracellular region

Traceable author statement. Source: Reactome

extracellular space

Traceable author statement PubMed 9548509. Source: ProtInc

neuronal cell body

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 23597562PubMed 23597562. Source: IntAct

Complete GO annotation...

Binary interactions

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform Beta (identifier: P20366-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Alpha (identifier: P20366-2)

The sequence of this isoform differs from the canonical sequence as follows:
     97-114: Missing.
     115-115: V → M
Isoform Gamma (identifier: P20366-3)

The sequence of this isoform differs from the canonical sequence as follows:
     74-88: Missing.
Isoform Delta (identifier: P20366-4)

The sequence of this isoform differs from the canonical sequence as follows:
     74-88: Missing.
     97-114: Missing.
     115-115: V → M

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Propeptide20 – 5637 Potential
PRO_0000033529
Peptide58 – 6811Substance P
PRO_0000033530
Peptide72 – 10736Neuropeptide K
PRO_0000033531
Peptide72 – 732Neuropeptide gamma, 1st part
PRO_0000033532
Peptide89 – 10719Neuropeptide gamma, 2nd part
PRO_0000033533
Peptide98 – 10710Neurokinin A Ref.10
PRO_0000033534
Peptide111 – 12616C-terminal-flanking peptide Ref.12
PRO_0000033535

Amino acid modifications

Modified residue681Methionine amide Ref.1
Modified residue1071Methionine amide Ref.1 Ref.10

Natural variations

Alternative sequence74 – 8815Missing in isoform Gamma and isoform Delta.
VSP_006375
Alternative sequence97 – 11418Missing in isoform Alpha and isoform Delta.
VSP_006376
Alternative sequence1151V → M in isoform Alpha and isoform Delta.
VSP_006377

Experimental info

Sequence conflict321S → P in BAD96677. Ref.4

Secondary structure

............. 129
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform Beta [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 51412C1692368DE4

FASTA12915,003
        10         20         30         40         50         60 
MKILVALAVF FLVSTQLFAE EIGANDDLNY WSDWYDSDQI KEELPEPFEH LLQRIARRPK 

        70         80         90        100        110        120 
PQQFFGLMGK RDADSSIEKQ VALLKALYGH GQISHKRHKT DSFVGLMGKR ALNSVAYERS 


AMQNYERRR 

« Hide

Isoform Alpha [UniParc].

Checksum: 28E051E5ACC42C47
Show »

FASTA11113,036
Isoform Gamma [UniParc].

Checksum: 499EECB627AECDE6
Show »

FASTA11413,343
Isoform Delta [UniParc].

Checksum: AE50A94DF2437361
Show »

FASTA9611,376

References

« Hide 'large scale' references
[1]"cDNA sequence of human beta-preprotachykinin, the common precursor to substance P and neurokinin A."
Harmar A.J., Armstrong A., Pascall J.C., Chapman K., Rosie R., Curtis A., Going J., Edwards C.R.W., Fink G.
FEBS Lett. 208:67-72(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA), AMIDATION AT MET-68 AND MET-107.
[2]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BETA).
[3]Tan A., Too H.P.
Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA).
Tissue: Brain.
[4]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BETA).
Tissue: Small intestine.
[5]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"Human chromosome 7: DNA sequence and biology."
Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. expand/collapse author list , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BETA).
Tissue: Brain.
[9]"Tachykinin (substance-P) gene expression in Leydig cells of the human and mouse testis."
Chiwakata C., Brackmann B., Hunt N., Davidoff M., Schulze W., Ivell R.
Endocrinology 128:2441-2448(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-122 (ISOFORMS BETA AND GAMMA).
Tissue: Testis.
[10]"Isolation and characterization of neurokinin A, neurokinin A(3-10) and neurokinin A(4-10) from a neutral water extract of a metastatic ileal carcinoid tumour."
Theodorsson-Norheim E., Joernvall H., Andersson M., Norheim I., Oeberg K., Jacobsson G.
Eur. J. Biochem. 166:693-697(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 98-107, AMIDATION AT MET-107.
[11]"Identification of a delta isoform of preprotachykinin mRNA in human mononuclear phagocytes and lymphocytes."
Lai J.P., Douglas S.D., Rappaport E., Wu J.M., Ho W.Z.
J. Neuroimmunol. 91:121-128(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-122 (ISOFORMS ALPHA; BETA AND DELTA).
Tissue: Blood and Brain.
[12]"Characterization of the C-terminal flanking peptide of human beta-preprotachykinin."
McGregor G.P., Conlon J.M.
Peptides 11:907-910(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 111-126.
Tissue: Adrenal medulla.
[13]"Three-dimensional structure of neuropeptide K bound to dodecylphosphocholine micelles."
Dike A., Cowsik S.M.
Biochemistry 45:2994-3004(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 72-107.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X54469 mRNA. Translation: CAA38351.1.
U37529 mRNA. Translation: AAA79195.1.
CR407602 mRNA. Translation: CAG28529.1.
CR541730 mRNA. Translation: CAG46531.1.
AK222957 mRNA. Translation: BAD96677.1.
AC004140 Genomic DNA. Translation: AAQ96888.1.
AC004140 Genomic DNA. Translation: AAQ96889.1.
AC004140 Genomic DNA. Translation: AAQ96890.1.
AC004140 Genomic DNA. Translation: AAQ96891.1.
CH236949 Genomic DNA. Translation: EAL24116.1.
CH236949 Genomic DNA. Translation: EAL24117.1.
CH236949 Genomic DNA. Translation: EAL24118.1.
CH236949 Genomic DNA. Translation: EAL24119.1.
CH471091 Genomic DNA. Translation: EAW76734.1.
CH471091 Genomic DNA. Translation: EAW76735.1.
CH471091 Genomic DNA. Translation: EAW76736.1.
CH471091 Genomic DNA. Translation: EAW76737.1.
BC018047 mRNA. Translation: AAH18047.1.
M68906 mRNA. Translation: AAA60159.1.
M68907 mRNA. Translation: AAA60160.1.
AF050655 mRNA. Translation: AAC15701.1.
AF050656 mRNA. Translation: AAC15702.1.
AF050658 mRNA. Translation: AAC15704.1.
CCDSCCDS5649.1. [P20366-1]
CCDS5650.1. [P20366-2]
CCDS5651.1. [P20366-3]
PIRSPHUB. A24805.
RefSeqNP_003173.1. NM_003182.2. [P20366-1]
NP_054702.1. NM_013996.2. [P20366-2]
NP_054703.1. NM_013997.2. [P20366-3]
NP_054704.1. NM_013998.2. [P20366-4]
UniGeneHs.2563.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2B19NMR-A72-107[»]
2KS9NMR-B58-68[»]
2KSANMR-B58-68[»]
2KSBNMR-B58-68[»]
4HOMX-ray1.90B58-68[»]
ProteinModelPortalP20366.
SMRP20366. Positions 72-107.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112727. 8 interactions.
IntActP20366. 9 interactions.
MINTMINT-1409908.
STRING9606.ENSP00000321106.

Chemistry

BindingDBP20366.
DrugBankDB00626. Bacitracin.

PTM databases

PhosphoSiteP20366.

Polymorphism databases

DMDM135886.

Proteomic databases

PaxDbP20366.
PRIDEP20366.

Protocols and materials databases

DNASU6863.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000319273; ENSP00000321106; ENSG00000006128. [P20366-1]
ENST00000346867; ENSP00000289574; ENSG00000006128. [P20366-3]
ENST00000350485; ENSP00000289576; ENSG00000006128. [P20366-2]
GeneID6863.
KEGGhsa:6863.
UCSCuc003uop.4. human. [P20366-1]
uc003uoq.4. human. [P20366-2]
uc003uor.4. human. [P20366-3]
uc003uos.4. human. [P20366-4]

Organism-specific databases

CTD6863.
GeneCardsGC07P097361.
HGNCHGNC:11517. TAC1.
HPACAB004588.
HPA014429.
MIM162320. gene.
neXtProtNX_P20366.
PharmGKBPA36297.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG40889.
HOVERGENHBG000127.
InParanoidP20366.
KOK05239.
OMAAYERNAM.
OrthoDBEOG7C2R3N.
PhylomeDBP20366.
TreeFamTF333405.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressP20366.
BgeeP20366.
CleanExHS_TAC1.
GenevestigatorP20366.

Family and domain databases

InterProIPR008216. Protachykinin.
IPR013055. Tachy_Neuro_lke_CS.
IPR008215. Tachykinin.
[Graphical view]
PANTHERPTHR11250. PTHR11250. 1 hit.
PRINTSPR01829. PROTACHYKNIN.
SMARTSM00203. TK. 2 hits.
[Graphical view]
PROSITEPS00267. TACHYKININ. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP20366.
GeneWikiTAC1.
GenomeRNAi6863.
NextBio26783.
PMAP-CutDBP20366.
PROP20366.
SOURCESearch...

Entry information

Entry nameTKN1_HUMAN
AccessionPrimary (citable) accession number: P20366
Secondary accession number(s): O60600 expand/collapse secondary AC list , O60601, Q00072, Q53GH4, Q549V0, Q549V1, Q549V2, Q6FHM1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 9, 2014
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM