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P20357 (MTAP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Microtubule-associated protein 2

Short name=MAP-2
Gene names
Name:Map2
Synonyms:Mtap2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1828 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

Subcellular location

Cytoplasmcytoskeleton Probable.

Post-translational modification

Phosphorylated at serine residues in K-X-G-S motifs by causing MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), detachment from microtubules, and their disassembly By similarity.

Sequence similarities

Contains 3 Tau/MAP repeats.

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoskeleton
Microtubule
   DomainRepeat
   LigandCalmodulin-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processaxonogenesis

Inferred from mutant phenotype PubMed 11891784. Source: MGI

cellular response to organic substance

Inferred from direct assay PubMed 18497889. Source: MGI

dendrite development

Inferred from mutant phenotype PubMed 11581286. Source: MGI

microtubule bundle formation

Inferred from mutant phenotype PubMed 11581286. Source: MGI

   Cellular_componentcell body

Inferred from direct assay PubMed 12074840PubMed 15255972. Source: MGI

cytoplasm

Inferred from direct assay PubMed 15656993. Source: MGI

dendrite

Inferred from direct assay PubMed 11834298PubMed 12074840PubMed 15255972PubMed 15834957PubMed 16980967PubMed 19208628PubMed 23861879PubMed 24035762PubMed 3147150. Source: MGI

dendritic shaft

Inferred from direct assay PubMed 15996550. Source: MGI

intracellular

Inferred from direct assay PubMed 16892058PubMed 18268009. Source: MGI

microtubule

Inferred from electronic annotation. Source: UniProtKB-KW

microtubule associated complex

Traceable author statement PubMed 11581286. Source: MGI

neuron projection

Inferred from direct assay PubMed 18359573. Source: MGI

neuronal cell body

Inferred from direct assay PubMed 15964665PubMed 18359573PubMed 19646951. Source: MGI

nuclear periphery

Inferred from direct assay PubMed 11834298. Source: MGI

   Molecular_functioncytoskeletal regulatory protein binding

Traceable author statement PubMed 11581286. Source: MGI

microtubule binding

Inferred from direct assay PubMed 17360631. Source: MGI

protein binding

Inferred from physical interaction PubMed 21385318. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Kndc1Q0KK559EBI-397863,EBI-8605532

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 18281828Microtubule-associated protein 2
PRO_0000072748

Regions

Repeat1662 – 169231Tau/MAP 1
Repeat1693 – 172331Tau/MAP 2
Repeat1724 – 175532Tau/MAP 3
Region1452 – 147221Calmodulin-binding Potential

Amino acid modifications

Modified residue7261Phosphoserine Ref.5
Modified residue13521Phosphoserine Ref.5 Ref.6
Modified residue13581Phosphothreonine Ref.5 Ref.6
Modified residue16061Phosphothreonine Ref.5
Modified residue16091Phosphothreonine Ref.5 Ref.6
Modified residue16801Phosphoserine; by MARK1 By similarity

Experimental info

Sequence conflict1161H → Y no nucleotide entry Ref.1
Sequence conflict1161H → Y in AAA39490. Ref.2
Sequence conflict4551K → E no nucleotide entry Ref.1
Sequence conflict4551K → E in AAA39490. Ref.2
Sequence conflict4591F → L no nucleotide entry Ref.1
Sequence conflict4591F → L in AAA39490. Ref.2
Sequence conflict6441D → G no nucleotide entry Ref.1
Sequence conflict6441D → G in AAA39490. Ref.2
Sequence conflict9381S → T no nucleotide entry Ref.1
Sequence conflict9381S → T in AAA39490. Ref.2
Sequence conflict9471R → G no nucleotide entry Ref.1
Sequence conflict9471R → G in AAA39490. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P20357 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: E8FA7621C45D5A0D

FASTA1,828199,132
        10         20         30         40         50         60 
MADERKDEGK APHWTSASLT EAAAHPHSPE MKDQGGAGEG LSRNANGFPY REEEEGAFGE 

        70         80         90        100        110        120 
HRSQGTYSDT KENGINGELT SADRETAEEV SARIVQVVTA EAVAVLKGEQ EKEAQHKDQP 

       130        140        150        160        170        180 
AALPLAAEET ANLPPSPPPS PASEQTATVE EDLLTASKME FPEQEKFPSS FAEPLDKGEM 

       190        200        210        220        230        240 
EFKMPSKPGE DFEHAALVPD TSKTPQDKKD LQGMEGEKLP PVPFAQTFGT NLEDRKQSTE 

       250        260        270        280        290        300 
PSIVMPSIGL SAEPPAPKEP KDWFIEMPTE SKKDEWGLAA PISPGPLTPM REKDVLEDIP 

       310        320        330        340        350        360 
RWEGKQFDSP MPSPFHGGSF TLPLDTMKNE RVSEGPRPFA PVFFQSDDKV SLQDPSALAT 

       370        380        390        400        410        420 
SKESSKDEEP LKDKADKVAD VSISEVTTLL GNVHSPVVEG YVGENISGEV KVTTDQEKKE 

       430        440        450        460        470        480 
TSAPSVQEPT LTETEPQTKL DEKSTVSIEE AVAKKEESFK LRDDKTGVIQ TSTEQSFSKE 

       490        500        510        520        530        540 
DQKGQEHTID ELKQDSFPIS LEQAVTDAAM TSKTLGKVTS EPEAVSERRE IQGLFEEKTA 

       550        560        570        580        590        600 
DKNKLEGAGS ATIAEVEMPF YEDKSGMSKY FETSALKEDM TRSTELGSDY YELSDSRGSA 

       610        620        630        640        650        660 
QESLDTISPK NQHDEKELQA KASQPSPPAQ EAGYSTLAQS YTPDHPSELP EEPSSPQERM 

       670        680        690        700        710        720 
FTIDPKVYGE KRDLHSKNKD DLTLSRSLGL GGRSAIEQRS MSINLPMSCL DSIALGFNFG 

       730        740        750        760        770        780 
RGHDLSPLAS DILTNTSGSM DEGDDYLPPT TPAVEKMPCF PIESKEEEDK AEQAKVTGGQ 

       790        800        810        820        830        840 
TIQVETSSES PFPAKEYYKN GTVMAPDLPE MLDLAGTRSR LASVSADAEV ARRKSVPSEA 

       850        860        870        880        890        900 
MLAESSTSLP PVADESPVTV KPDSQLEDMG YCVFNKYTVP LPSPVQDSEN LSGESGSFYE 

       910        920        930        940        950        960 
GTDDKVRRDL ATDLSLIEVK LAAAGRVKDE FTAEKEASPP TSADKSRLSR EFDHDRKAND 

       970        980        990       1000       1010       1020 
KLDTVLEKSE EHIDSKEHAK ESEEMGGKVE LFGLGITYDQ ASTKELITTK DTSPEKTEKG 

      1030       1040       1050       1060       1070       1080 
LSSVPEVAEV EPTTKADQGL DFAATKAEPS QLDIKVSDFG QMASGMNVDA GKAIELKFEV 

      1090       1100       1110       1120       1130       1140 
AQELTLSSEA PQEADSFMGV ESGHIKEGGK VNETEVKEKV TKPDLVHQEA VDKEESYESS 

      1150       1160       1170       1180       1190       1200 
GEHESLTMES LKPDEGKKET SPETSLIQDE VALKLSVEIP CPPPVSEADL STDEKGEVQM 

      1210       1220       1230       1240       1250       1260 
EFIQLPKEES TETPDIPAIP SDVTQPQPEA IVSEPAEVPS EEEEIEAGGE YDKLLFRSDT 

      1270       1280       1290       1300       1310       1320 
LQISDLLVSE SREEFVETCP GELKGVVESV VTIEDDFITV VQTTTDEGES GSHSVRFAAP 

      1330       1340       1350       1360       1370       1380 
AQPEEERRPR PHDEELEIEM AAEAQAEPKD GSPDAPATPE KEEVAFSEYK TETYDDYKDE 

      1390       1400       1410       1420       1430       1440 
TTIDDSIMDA DSLWVDTQDD DRSILTEQLE TIPKEERAEK DARRPSLEKH RKEKPFKTGR 

      1450       1460       1470       1480       1490       1500 
GRISTPERKV AKKEPSTVSR DEVRRKKAVY KKAELAKKSE VQAHSPSRKL ILKPAIKYTR 

      1510       1520       1530       1540       1550       1560 
PTHLSCVKRK TTAASGDLAQ APGAFKQAKD KVTDGISKSP EKRSSLPRPS SILPPRRGVS 

      1570       1580       1590       1600       1610       1620 
GDREENSFSL NSSISSARRT TRSEPIRRAG KSGTSTPTTP GSTAITPGTP PSYSSRTPGT 

      1630       1640       1650       1660       1670       1680 
PGTPSYPRTP GTPKSGILVP SEKKVAIIRT PPKSPATPKQ LRLINQPLPD LKNVKSKIGS 

      1690       1700       1710       1720       1730       1740 
TDNIKYQPKG GQVQIVTKKI DLSHVTSKCG SLKNIRHRPG GGRVKIESVK LDFKEKAQAK 

      1750       1760       1770       1780       1790       1800 
VGSLDNAHHV PGGGNVKIDS QKLNFREHAK ARVDHGAEII TQSPSRSSVA SPRRLSNVSS 

      1810       1820 
SGSINLLESP QLATLAEDVT AALAKQGL 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequence of a cDNA encoding mouse MAP2."
Wang D., Lewis S.A., Cowan N.J.
Nucleic Acids Res. 16:11369-11370(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Microtubule-associated protein MAP2 shares a microtubule binding motif with tau protein."
Lewis S.A., Wang D., Cowan N.J.
Science 242:936-939(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 94-107; 583-597; 909-920; 989-1004; 1159-1174; 1403-1414 AND 1511-1538, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
[5]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-726; SER-1352; THR-1358; THR-1606 AND THR-1609, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
[6]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1352; THR-1358 AND THR-1609, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[7]"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain cortex.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M21041 mRNA. Translation: AAA39490.1.
AC091465 Genomic DNA. No translation available.
PIRA40115.
RefSeqXP_006495817.1. XM_006495754.1.
XP_006495818.1. XM_006495755.1.
UniGeneMm.256966.

3D structure databases

ProteinModelPortalP20357.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP20357. 17 interactions.
MINTMINT-4101182.

PTM databases

PhosphoSiteP20357.

Proteomic databases

MaxQBP20357.
PaxDbP20357.
PRIDEP20357.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000114013; ENSMUSP00000109646; ENSMUSG00000015222.
GeneID17756.
KEGGmmu:17756.
UCSCuc007bhz.1. mouse.

Organism-specific databases

CTD4133.
MGIMGI:97175. Map2.

Phylogenomic databases

eggNOGNOG148882.
GeneTreeENSGT00530000063491.
HOGENOMHOG000113477.
HOVERGENHBG006322.
InParanoidP20357.
KOK10430.
OMAKTETYDD.
OrthoDBEOG75XGKK.

Gene expression databases

ArrayExpressP20357.
BgeeP20357.
CleanExMM_MTAP2.
GenevestigatorP20357.

Family and domain databases

InterProIPR027324. MAP2/MAP4/Tau.
IPR013588. MAP2_projctn.
IPR001084. MAP_tubulin-bd_rpt.
[Graphical view]
PANTHERPTHR11501. PTHR11501. 1 hit.
PfamPF08377. MAP2_projctn. 1 hit.
PF00418. Tubulin-binding. 3 hits.
[Graphical view]
PROSITEPS00229. TAU_MAP_1. 2 hits.
PS51491. TAU_MAP_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

PROP20357.
SOURCESearch...

Entry information

Entry nameMTAP2_MOUSE
AccessionPrimary (citable) accession number: P20357
Secondary accession number(s): E9QLE1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot