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Protein

Tissue factor

Gene

F3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

GO - Molecular functioni

  1. cytokine receptor activity Source: GO_Central
  2. phospholipid binding Source: MGI
  3. protease binding Source: MGI

GO - Biological processi

  1. activation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  2. activation of plasma proteins involved in acute inflammatory response Source: MGI
  3. blood coagulation Source: MGI
  4. cytokine-mediated signaling pathway Source: GO_Central
  5. positive regulation of angiogenesis Source: MGI
  6. positive regulation of endothelial cell proliferation Source: MGI
  7. positive regulation of platelet-derived growth factor receptor signaling pathway Source: MGI
  8. positive regulation of protein kinase B signaling Source: MGI
Complete GO annotation...

Keywords - Biological processi

Blood coagulation, Hemostasis

Enzyme and pathway databases

ReactomeiREACT_240293. Extrinsic Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Tissue factor
Short name:
TF
Alternative name(s):
Coagulation factor III
CD_antigen: CD142
Gene namesi
Name:F3
Synonyms:Cf-3, Cf3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:88381. F3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini29 – 251223ExtracellularSequence AnalysisAdd
BLAST
Transmembranei252 – 27423HelicalSequence AnalysisAdd
BLAST
Topological domaini275 – 29420CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell surface Source: MGI
  2. extracellular matrix Source: MGI
  3. extracellular space Source: MGI
  4. extracellular vesicular exosome Source: MGI
  5. integral component of membrane Source: GO_Central
  6. plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Add
BLAST
Chaini29 – 294266Tissue factorPRO_0000033639Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi37 – 371N-linked (GlcNAc...)1 Publication
Glycosylationi57 – 571N-linked (GlcNAc...)1 Publication
Disulfide bondi75 ↔ 83By similarity
Glycosylationi169 – 1691N-linked (GlcNAc...)Sequence Analysis
Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi218 ↔ 241By similarity
Lipidationi275 – 2751S-palmitoyl cysteineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

MaxQBiP20352.
PaxDbiP20352.
PRIDEiP20352.

PTM databases

PhosphoSiteiP20352.

Expressioni

Inductioni

Expression in the liver oscillates in a circadian manner.1 Publication

Gene expression databases

BgeeiP20352.
CleanExiMM_F3.
GenevestigatoriP20352.

Interactioni

Subunit structurei

Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII.By similarity

Protein-protein interaction databases

IntActiP20352. 1 interaction.
MINTiMINT-4137612.

Structurei

3D structure databases

ProteinModelPortaliP20352.
SMRiP20352. Positions 35-244.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi245 – 2473WKS motif

Sequence similaritiesi

Belongs to the tissue factor family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG43483.
GeneTreeiENSGT00390000012668.
HOGENOMiHOG000043076.
HOVERGENiHBG005051.
InParanoidiP20352.
KOiK03901.
OrthoDBiEOG7C2R28.
PhylomeDBiP20352.
TreeFamiTF352627.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR015373. Interferon_alpha/beta_rcpt_bsu.
IPR016354. Tissue_fac/coagulation_fac-3.
IPR001187. Tissue_factor.
[Graphical view]
PfamiPF09294. Interfer-bind. 1 hit.
PF01108. Tissue_fac. 1 hit.
[Graphical view]
PIRSFiPIRSF002498. Tissue_factor_3. 1 hit.
PRINTSiPR00346. TISSUEFACTOR.
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS00621. TISSUE_FACTOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20352-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAILVRPRLL AALAPTFLGC LLLQVIAGAG IPEKAFNLTW ISTDFKTILE
60 70 80 90 100
WQPKPTNYTY TVQISDRSRN WKNKCFSTTD TECDLTDEIV KDVTWAYEAK
110 120 130 140 150
VLSVPRRNSV HGDGDQLVIH GEEPPFTNAP KFLPYRDTNL GQPVIQQFEQ
160 170 180 190 200
DGRKLNVVVK DSLTLVRKNG TFLTLRQVFG KDLGYIITYR KGSSTGKKTN
210 220 230 240 250
ITNTNEFSID VEEGVSYCFF VQAMIFSRKT NQNSPGSSTV CTEQWKSFLG
260 270 280 290
ETLIIVGAVV LLATIFIILL SISLCKRRKN RAGQKGKNTP SRLA
Length:294
Mass (Da):32,935
Last modified:August 1, 1991 - v2
Checksum:iA306101293C31FA0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261I → T in AAA40414. (PubMed:2761539)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57896 mRNA. Translation: AAA63400.1.
M26071 mRNA. Translation: AAA40414.1.
BC016397 mRNA. Translation: AAH16397.1.
CCDSiCCDS17805.1.
PIRiA32318. KFMS3.
RefSeqiNP_034301.3. NM_010171.3.
UniGeneiMm.273188.

Genome annotation databases

EnsembliENSMUST00000029771; ENSMUSP00000029771; ENSMUSG00000028128.
GeneIDi14066.
KEGGimmu:14066.
UCSCiuc008ree.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57896 mRNA. Translation: AAA63400.1.
M26071 mRNA. Translation: AAA40414.1.
BC016397 mRNA. Translation: AAH16397.1.
CCDSiCCDS17805.1.
PIRiA32318. KFMS3.
RefSeqiNP_034301.3. NM_010171.3.
UniGeneiMm.273188.

3D structure databases

ProteinModelPortaliP20352.
SMRiP20352. Positions 35-244.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP20352. 1 interaction.
MINTiMINT-4137612.

Chemistry

ChEMBLiCHEMBL5787.

PTM databases

PhosphoSiteiP20352.

Proteomic databases

MaxQBiP20352.
PaxDbiP20352.
PRIDEiP20352.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029771; ENSMUSP00000029771; ENSMUSG00000028128.
GeneIDi14066.
KEGGimmu:14066.
UCSCiuc008ree.2. mouse.

Organism-specific databases

CTDi2152.
MGIiMGI:88381. F3.

Phylogenomic databases

eggNOGiNOG43483.
GeneTreeiENSGT00390000012668.
HOGENOMiHOG000043076.
HOVERGENiHBG005051.
InParanoidiP20352.
KOiK03901.
OrthoDBiEOG7C2R28.
PhylomeDBiP20352.
TreeFamiTF352627.

Enzyme and pathway databases

ReactomeiREACT_240293. Extrinsic Pathway.

Miscellaneous databases

NextBioi285048.
PROiP20352.
SOURCEiSearch...

Gene expression databases

BgeeiP20352.
CleanExiMM_F3.
GenevestigatoriP20352.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR015373. Interferon_alpha/beta_rcpt_bsu.
IPR016354. Tissue_fac/coagulation_fac-3.
IPR001187. Tissue_factor.
[Graphical view]
PfamiPF09294. Interfer-bind. 1 hit.
PF01108. Tissue_fac. 1 hit.
[Graphical view]
PIRSFiPIRSF002498. Tissue_factor_3. 1 hit.
PRINTSiPR00346. TISSUEFACTOR.
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS00621. TISSUE_FACTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of murine tissue factor and regulation of gene expression by transforming growth factor type beta 1."
    Ranganathan G., Blatti S.P., Subramaniam M., Fass D.N., Maihle N.J., Getz M.J.
    J. Biol. Chem. 266:496-501(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "A growth factor-responsive gene of murine BALB/c 3T3 cells encodes a protein homologous to human tissue factor."
    Hartzell S., Ryder K., Lanahan A., Lau L.F., Nathans D.
    Mol. Cell. Biol. 9:2567-2573(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  4. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
    Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
    Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-37 AND ASN-57.
    Tissue: Myoblast.
  5. "The molecular clock regulates circadian transcription of tissue factor gene."
    Oishi K., Koyanagi S., Ohkura N.
    Biochem. Biophys. Res. Commun. 431:332-335(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiTF_MOUSE
AccessioniPrimary (citable) accession number: P20352
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: August 1, 1991
Last modified: February 4, 2015
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.