Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P20350

- RHOM_DROME

UniProt

P20350 - RHOM_DROME

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Protein rhomboid

Gene

rho

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts early in embryonic development to establish position along the dorsoventral axis and then again later to specify the fate of neuronal precursor cells. Involved in EGF receptor signaling; cleaves Spitz to release the active growth factor.2 Publications

Catalytic activityi

Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei217 – 2171Nucleophile
Active sitei281 – 2811

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: FlyBase

GO - Biological processi

  1. axonogenesis Source: FlyBase
  2. behavioral response to ethanol Source: FlyBase
  3. brain development Source: FlyBase
  4. branched duct epithelial cell fate determination, open tracheal system Source: FlyBase
  5. determination of genital disc primordium Source: FlyBase
  6. epidermal growth factor receptor signaling pathway Source: FlyBase
  7. epithelial cell proliferation involved in Malpighian tubule morphogenesis Source: FlyBase
  8. imaginal disc-derived wing morphogenesis Source: FlyBase
  9. imaginal disc-derived wing vein morphogenesis Source: FlyBase
  10. imaginal disc-derived wing vein specification Source: FlyBase
  11. larval salivary gland morphogenesis Source: FlyBase
  12. learning or memory Source: FlyBase
  13. leg disc proximal/distal pattern formation Source: FlyBase
  14. maintenance of epithelial integrity, open tracheal system Source: FlyBase
  15. mitotic cytokinesis Source: FlyBase
  16. morphogenesis of a branching structure Source: FlyBase
  17. negative regulation of gene expression Source: FlyBase
  18. oenocyte development Source: FlyBase
  19. olfactory learning Source: FlyBase
  20. open tracheal system development Source: FlyBase
  21. ovarian follicle cell development Source: FlyBase
  22. peptide bond cleavage involved in epidermal growth factor receptor ligand maturation Source: FlyBase
  23. peripheral nervous system development Source: FlyBase
  24. positive regulation of epidermal growth factor receptor signaling pathway Source: FlyBase
  25. regulation of epidermal growth factor receptor signaling pathway Source: UniProtKB
  26. salivary gland boundary specification Source: FlyBase
  27. salivary gland development Source: FlyBase
  28. single organismal cell-cell adhesion Source: FlyBase
  29. stem cell fate commitment Source: FlyBase
  30. tracheal pit formation in open tracheal system Source: FlyBase
  31. wing cell fate specification Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Protease, Serine protease

Enzyme and pathway databases

SignaLinkiP20350.

Protein family/group databases

MEROPSiS54.001.
TCDBi9.B.104.2.1. the rhomboid protease (rhomboid) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein rhomboid (EC:3.4.21.105)
Alternative name(s):
Protein veinlet
Gene namesi
Name:rho
Synonyms:Ve
ORF Names:CG1004
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0004635. rho.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9898CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei99 – 11921HelicalSequence AnalysisAdd
BLAST
Topological domaini120 – 16243LumenalSequence AnalysisAdd
BLAST
Transmembranei163 – 18321HelicalSequence AnalysisAdd
BLAST
Topological domaini184 – 1885CytoplasmicSequence Analysis
Transmembranei189 – 20921HelicalSequence AnalysisAdd
BLAST
Topological domaini210 – 2101LumenalSequence Analysis
Transmembranei211 – 23121HelicalSequence AnalysisAdd
BLAST
Topological domaini232 – 24413CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei245 – 26521HelicalSequence AnalysisAdd
BLAST
Topological domaini266 – 27510LumenalSequence Analysis
Transmembranei276 – 29621HelicalSequence AnalysisAdd
BLAST
Topological domaini297 – 30812CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei309 – 32921HelicalSequence AnalysisAdd
BLAST
Topological domaini330 – 35526LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. apical part of cell Source: FlyBase
  2. Golgi apparatus Source: FlyBase
  3. integral component of membrane Source: FlyBase
  4. plasma membrane Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi151 – 1511W → A: Abolishes protease activity. 1 Publication
Mutagenesisi152 – 1521R → A: Abolishes protease activity. 1 Publication
Mutagenesisi169 – 1691N → A: Abolishes protease activity. 1 Publication
Mutagenesisi215 – 2151G → A: Abolishes protease activity. 1 Publication
Mutagenesisi216 – 2161A → P: Abolishes protease activity. 1 Publication
Mutagenesisi217 – 2171S → A, C or T: Abolishes protease activity. 2 Publications
Mutagenesisi281 – 2811H → A: Abolishes protease activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 355355Protein rhomboidPRO_0000206179Add
BLAST

Proteomic databases

PRIDEiP20350.

Expressioni

Developmental stagei

Early blastoderm stages and later during nervous development.

Gene expression databases

BgeeiP20350.
ExpressionAtlasiP20350. differential.

Interactioni

Protein-protein interaction databases

BioGridi63708. 27 interactions.
IntActiP20350. 1 interaction.
MINTiMINT-4508920.

Structurei

3D structure databases

ProteinModelPortaliP20350.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S54 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG300180.
InParanoidiP20350.
KOiK02857.
OMAiELGFFTY.
OrthoDBiEOG7SN8CK.
PhylomeDBiP20350.

Family and domain databases

Gene3Di1.20.1540.10. 1 hit.
InterProiIPR002610. Peptidase_S54_rhomboid.
IPR022764. Peptidase_S54_rhomboid_dom.
IPR017213. Peptidase_S54_rhomboid_met.
[Graphical view]
PANTHERiPTHR22936. PTHR22936. 1 hit.
PfamiPF01694. Rhomboid. 1 hit.
[Graphical view]
PIRSFiPIRSF037470. Rhomboid. 1 hit.

Sequencei

Sequence statusi: Complete.

P20350-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MENLTQNVNE TKVDLGQEKE KEASQEEEHA TAAKETIIDI PAACSSSSNS
60 70 80 90 100
SSYDTDCSTA SSTCCTRQGE HIYMQREAIP ATPLPESEDI GLLKYVHRQH
110 120 130 140 150
WPWFILVISI IEIAIFAYDR YTMPAQNFGL PVPIPSDSVL VYRPDRRLQV
160 170 180 190 200
WRFFSYMFLH ANWFHLGFNI VIQLFFGIPL EVMHGTARIG VIYMAGVFAG
210 220 230 240 250
SLGTSVVDSE VFLVGASGGV YALLAAHLAN ITLNYAHMKS ASTQLGSVVI
260 270 280 290 300
FVSCDLGYAL YTQYFDGSAF AKGPQVSYIA HLTGALAGLT IGFLVLKNFG
310 320 330 340 350
HREYEQLIWW LALGVYCAFT VFAIVFNLIN TVTAQLMEEQ GEVITQHLLH

DLGVS
Length:355
Mass (Da):39,330
Last modified:March 5, 2002 - v2
Checksum:i2E0572D08831C5D0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41L → P in CAA36692. (PubMed:2110920)Curated
Sequence conflicti33 – 331A → V in CAA36692. (PubMed:2110920)Curated
Sequence conflicti46 – 461S → T in CAA36692. (PubMed:2110920)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52454 mRNA. Translation: CAA36692.1.
AE014296 Genomic DNA. Translation: AAF47496.1.
PIRiA34597.
RefSeqiNP_001261255.1. NM_001274326.1.
NP_523883.2. NM_079159.3.
UniGeneiDm.808.

Genome annotation databases

EnsemblMetazoaiFBtr0072694; FBpp0072578; FBgn0004635.
FBtr0333207; FBpp0305409; FBgn0004635.
GeneIDi38168.
KEGGidme:Dmel_CG1004.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52454 mRNA. Translation: CAA36692.1 .
AE014296 Genomic DNA. Translation: AAF47496.1 .
PIRi A34597.
RefSeqi NP_001261255.1. NM_001274326.1.
NP_523883.2. NM_079159.3.
UniGenei Dm.808.

3D structure databases

ProteinModelPortali P20350.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 63708. 27 interactions.
IntActi P20350. 1 interaction.
MINTi MINT-4508920.

Protein family/group databases

MEROPSi S54.001.
TCDBi 9.B.104.2.1. the rhomboid protease (rhomboid) family.

Proteomic databases

PRIDEi P20350.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0072694 ; FBpp0072578 ; FBgn0004635 .
FBtr0333207 ; FBpp0305409 ; FBgn0004635 .
GeneIDi 38168.
KEGGi dme:Dmel_CG1004.

Organism-specific databases

CTDi 6010.
FlyBasei FBgn0004635. rho.

Phylogenomic databases

eggNOGi NOG300180.
InParanoidi P20350.
KOi K02857.
OMAi ELGFFTY.
OrthoDBi EOG7SN8CK.
PhylomeDBi P20350.

Enzyme and pathway databases

SignaLinki P20350.

Miscellaneous databases

ChiTaRSi rho. fly.
GenomeRNAii 38168.
NextBioi 807306.
PROi P20350.

Gene expression databases

Bgeei P20350.
ExpressionAtlasi P20350. differential.

Family and domain databases

Gene3Di 1.20.1540.10. 1 hit.
InterProi IPR002610. Peptidase_S54_rhomboid.
IPR022764. Peptidase_S54_rhomboid_dom.
IPR017213. Peptidase_S54_rhomboid_met.
[Graphical view ]
PANTHERi PTHR22936. PTHR22936. 1 hit.
Pfami PF01694. Rhomboid. 1 hit.
[Graphical view ]
PIRSFi PIRSF037470. Rhomboid. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Rhomboid, a gene required for dorsoventral axis establishment and peripheral nervous system development in Drosophila melanogaster."
    Bier E., Jan L.Y., Jan Y.N.
    Genes Dev. 4:190-203(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. "Drosophila Rhomboid-1 defines a family of putative intramembrane serine proteases."
    Urban S., Lee J.R., Freeman M.
    Cell 107:173-182(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF TRP-151; ARG-152; ASN-169; GLY-215; SER-217 AND HIS-281.
  5. "Rhomboid family pseudoproteases use the ER quality control machinery to regulate intercellular signaling."
    Zettl M., Adrain C., Strisovsky K., Lastun V., Freeman M.
    Cell 145:79-91(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ALA-216 AND SER-217.

Entry informationi

Entry nameiRHOM_DROME
AccessioniPrimary (citable) accession number: P20350
Secondary accession number(s): Q9W0F2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: March 5, 2002
Last modified: November 26, 2014
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3