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P20350

- RHOM_DROME

UniProt

P20350 - RHOM_DROME

Protein

Protein rhomboid

Gene

rho

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 2 (05 Mar 2002)
      Previous versions | rss
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    Functioni

    Acts early in embryonic development to establish position along the dorsoventral axis and then again later to specify the fate of neuronal precursor cells. Involved in EGF receptor signaling; cleaves Spitz to release the active growth factor.2 Publications

    Catalytic activityi

    Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei217 – 2171Nucleophile
    Active sitei281 – 2811

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: FlyBase

    GO - Biological processi

    1. axonogenesis Source: FlyBase
    2. behavioral response to ethanol Source: FlyBase
    3. brain development Source: FlyBase
    4. branched duct epithelial cell fate determination, open tracheal system Source: FlyBase
    5. determination of genital disc primordium Source: FlyBase
    6. epidermal growth factor receptor signaling pathway Source: FlyBase
    7. epithelial cell proliferation involved in Malpighian tubule morphogenesis Source: FlyBase
    8. imaginal disc-derived wing morphogenesis Source: FlyBase
    9. imaginal disc-derived wing vein morphogenesis Source: FlyBase
    10. imaginal disc-derived wing vein specification Source: FlyBase
    11. larval salivary gland morphogenesis Source: FlyBase
    12. learning or memory Source: FlyBase
    13. leg disc proximal/distal pattern formation Source: FlyBase
    14. maintenance of epithelial integrity, open tracheal system Source: FlyBase
    15. morphogenesis of a branching structure Source: FlyBase
    16. negative regulation of gene expression Source: FlyBase
    17. oenocyte development Source: FlyBase
    18. olfactory learning Source: FlyBase
    19. open tracheal system development Source: FlyBase
    20. ovarian follicle cell development Source: FlyBase
    21. peptide bond cleavage involved in epidermal growth factor receptor ligand maturation Source: FlyBase
    22. peripheral nervous system development Source: FlyBase
    23. positive regulation of epidermal growth factor receptor signaling pathway Source: FlyBase
    24. regulation of epidermal growth factor receptor signaling pathway Source: UniProtKB
    25. salivary gland boundary specification Source: FlyBase
    26. salivary gland development Source: FlyBase
    27. single organismal cell-cell adhesion Source: FlyBase
    28. stem cell fate commitment Source: FlyBase
    29. tracheal pit formation in open tracheal system Source: FlyBase
    30. wing cell fate specification Source: FlyBase

    Keywords - Molecular functioni

    Developmental protein, Hydrolase, Protease, Serine protease

    Enzyme and pathway databases

    SignaLinkiP20350.

    Protein family/group databases

    MEROPSiS54.001.
    TCDBi9.B.104.2.1. the rhomboid protease (rhomboid) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein rhomboid (EC:3.4.21.105)
    Alternative name(s):
    Protein veinlet
    Gene namesi
    Name:rho
    Synonyms:Ve
    ORF Names:CG1004
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3L

    Organism-specific databases

    FlyBaseiFBgn0004635. rho.

    Subcellular locationi

    GO - Cellular componenti

    1. apical part of cell Source: FlyBase
    2. Golgi apparatus Source: FlyBase
    3. Golgi membrane Source: UniProtKB-SubCell
    4. integral component of membrane Source: FlyBase
    5. plasma membrane Source: FlyBase

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi151 – 1511W → A: Abolishes protease activity. 1 Publication
    Mutagenesisi152 – 1521R → A: Abolishes protease activity. 1 Publication
    Mutagenesisi169 – 1691N → A: Abolishes protease activity. 1 Publication
    Mutagenesisi215 – 2151G → A: Abolishes protease activity. 1 Publication
    Mutagenesisi216 – 2161A → P: Abolishes protease activity. 1 Publication
    Mutagenesisi217 – 2171S → A, C or T: Abolishes protease activity. 2 Publications
    Mutagenesisi281 – 2811H → A: Abolishes protease activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 355355Protein rhomboidPRO_0000206179Add
    BLAST

    Proteomic databases

    PRIDEiP20350.

    Expressioni

    Developmental stagei

    Early blastoderm stages and later during nervous development.

    Gene expression databases

    BgeeiP20350.

    Interactioni

    Protein-protein interaction databases

    BioGridi63708. 27 interactions.
    IntActiP20350. 1 interaction.
    MINTiMINT-4508920.

    Structurei

    3D structure databases

    ProteinModelPortaliP20350.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 9898CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini120 – 16243LumenalSequence AnalysisAdd
    BLAST
    Topological domaini184 – 1885CytoplasmicSequence Analysis
    Topological domaini210 – 2101LumenalSequence Analysis
    Topological domaini232 – 24413CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini266 – 27510LumenalSequence Analysis
    Topological domaini297 – 30812CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini330 – 35526LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei99 – 11921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei163 – 18321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei189 – 20921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei211 – 23121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei245 – 26521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei276 – 29621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei309 – 32921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S54 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG300180.
    InParanoidiP20350.
    KOiK02857.
    OMAiELGFFTY.
    OrthoDBiEOG7SN8CK.
    PhylomeDBiP20350.

    Family and domain databases

    Gene3Di1.20.1540.10. 1 hit.
    InterProiIPR002610. Peptidase_S54_rhomboid.
    IPR022764. Peptidase_S54_rhomboid_dom.
    IPR017213. Peptidase_S54_rhomboid_met.
    [Graphical view]
    PANTHERiPTHR22936. PTHR22936. 1 hit.
    PfamiPF01694. Rhomboid. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037470. Rhomboid. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P20350-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENLTQNVNE TKVDLGQEKE KEASQEEEHA TAAKETIIDI PAACSSSSNS    50
    SSYDTDCSTA SSTCCTRQGE HIYMQREAIP ATPLPESEDI GLLKYVHRQH 100
    WPWFILVISI IEIAIFAYDR YTMPAQNFGL PVPIPSDSVL VYRPDRRLQV 150
    WRFFSYMFLH ANWFHLGFNI VIQLFFGIPL EVMHGTARIG VIYMAGVFAG 200
    SLGTSVVDSE VFLVGASGGV YALLAAHLAN ITLNYAHMKS ASTQLGSVVI 250
    FVSCDLGYAL YTQYFDGSAF AKGPQVSYIA HLTGALAGLT IGFLVLKNFG 300
    HREYEQLIWW LALGVYCAFT VFAIVFNLIN TVTAQLMEEQ GEVITQHLLH 350
    DLGVS 355
    Length:355
    Mass (Da):39,330
    Last modified:March 5, 2002 - v2
    Checksum:i2E0572D08831C5D0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti4 – 41L → P in CAA36692. (PubMed:2110920)Curated
    Sequence conflicti33 – 331A → V in CAA36692. (PubMed:2110920)Curated
    Sequence conflicti46 – 461S → T in CAA36692. (PubMed:2110920)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52454 mRNA. Translation: CAA36692.1.
    AE014296 Genomic DNA. Translation: AAF47496.1.
    PIRiA34597.
    RefSeqiNP_001261255.1. NM_001274326.1.
    NP_523883.2. NM_079159.3.
    UniGeneiDm.808.

    Genome annotation databases

    EnsemblMetazoaiFBtr0072694; FBpp0072578; FBgn0004635.
    FBtr0333207; FBpp0305409; FBgn0004635.
    GeneIDi38168.
    KEGGidme:Dmel_CG1004.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52454 mRNA. Translation: CAA36692.1 .
    AE014296 Genomic DNA. Translation: AAF47496.1 .
    PIRi A34597.
    RefSeqi NP_001261255.1. NM_001274326.1.
    NP_523883.2. NM_079159.3.
    UniGenei Dm.808.

    3D structure databases

    ProteinModelPortali P20350.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 63708. 27 interactions.
    IntActi P20350. 1 interaction.
    MINTi MINT-4508920.

    Protein family/group databases

    MEROPSi S54.001.
    TCDBi 9.B.104.2.1. the rhomboid protease (rhomboid) family.

    Proteomic databases

    PRIDEi P20350.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0072694 ; FBpp0072578 ; FBgn0004635 .
    FBtr0333207 ; FBpp0305409 ; FBgn0004635 .
    GeneIDi 38168.
    KEGGi dme:Dmel_CG1004.

    Organism-specific databases

    CTDi 6010.
    FlyBasei FBgn0004635. rho.

    Phylogenomic databases

    eggNOGi NOG300180.
    InParanoidi P20350.
    KOi K02857.
    OMAi ELGFFTY.
    OrthoDBi EOG7SN8CK.
    PhylomeDBi P20350.

    Enzyme and pathway databases

    SignaLinki P20350.

    Miscellaneous databases

    ChiTaRSi RHO. drosophila.
    GenomeRNAii 38168.
    NextBioi 807306.
    PROi P20350.

    Gene expression databases

    Bgeei P20350.

    Family and domain databases

    Gene3Di 1.20.1540.10. 1 hit.
    InterProi IPR002610. Peptidase_S54_rhomboid.
    IPR022764. Peptidase_S54_rhomboid_dom.
    IPR017213. Peptidase_S54_rhomboid_met.
    [Graphical view ]
    PANTHERi PTHR22936. PTHR22936. 1 hit.
    Pfami PF01694. Rhomboid. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037470. Rhomboid. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Rhomboid, a gene required for dorsoventral axis establishment and peripheral nervous system development in Drosophila melanogaster."
      Bier E., Jan L.Y., Jan Y.N.
      Genes Dev. 4:190-203(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    4. "Drosophila Rhomboid-1 defines a family of putative intramembrane serine proteases."
      Urban S., Lee J.R., Freeman M.
      Cell 107:173-182(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF TRP-151; ARG-152; ASN-169; GLY-215; SER-217 AND HIS-281.
    5. "Rhomboid family pseudoproteases use the ER quality control machinery to regulate intercellular signaling."
      Zettl M., Adrain C., Strisovsky K., Lastun V., Freeman M.
      Cell 145:79-91(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF ALA-216 AND SER-217.

    Entry informationi

    Entry nameiRHOM_DROME
    AccessioniPrimary (citable) accession number: P20350
    Secondary accession number(s): Q9W0F2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: March 5, 2002
    Last modified: October 1, 2014
    This is version 138 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3