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Protein

Protein rhomboid

Gene

rho

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts early in embryonic development to establish position along the dorsoventral axis and then again later to specify the fate of neuronal precursor cells. Involved in EGF receptor signaling; cleaves Spitz to release the active growth factor.2 Publications

Catalytic activityi

Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei217Nucleophile1
Active sitei2811

GO - Molecular functioni

  • serine-type endopeptidase activity Source: FlyBase

GO - Biological processi

  • axonogenesis Source: FlyBase
  • behavioral response to ethanol Source: FlyBase
  • brain development Source: FlyBase
  • branched duct epithelial cell fate determination, open tracheal system Source: FlyBase
  • determination of genital disc primordium Source: FlyBase
  • epidermal growth factor receptor signaling pathway Source: FlyBase
  • epithelial cell proliferation involved in Malpighian tubule morphogenesis Source: FlyBase
  • imaginal disc-derived wing morphogenesis Source: FlyBase
  • imaginal disc-derived wing vein morphogenesis Source: FlyBase
  • imaginal disc-derived wing vein specification Source: FlyBase
  • larval salivary gland morphogenesis Source: FlyBase
  • learning or memory Source: FlyBase
  • leg disc proximal/distal pattern formation Source: FlyBase
  • maintenance of epithelial integrity, open tracheal system Source: FlyBase
  • mitotic cytokinesis Source: FlyBase
  • morphogenesis of a branching structure Source: FlyBase
  • negative regulation of gene expression Source: FlyBase
  • oenocyte development Source: FlyBase
  • olfactory learning Source: FlyBase
  • open tracheal system development Source: FlyBase
  • ovarian follicle cell development Source: FlyBase
  • peptide bond cleavage involved in epidermal growth factor receptor ligand maturation Source: FlyBase
  • peripheral nervous system development Source: FlyBase
  • positive regulation of epidermal growth factor receptor signaling pathway Source: FlyBase
  • regulation of epidermal growth factor receptor signaling pathway Source: UniProtKB
  • salivary gland boundary specification Source: FlyBase
  • salivary gland development Source: FlyBase
  • single organismal cell-cell adhesion Source: FlyBase
  • stem cell fate commitment Source: FlyBase
  • tracheal pit formation in open tracheal system Source: FlyBase
  • wing cell fate specification Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Protease, Serine protease

Enzyme and pathway databases

SignaLinkiP20350.

Protein family/group databases

MEROPSiS54.001.
TCDBi9.B.104.2.1. the rhomboid protease (rhomboid) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein rhomboid (EC:3.4.21.105)
Alternative name(s):
Protein veinlet
Gene namesi
Name:rho
Synonyms:Ve
ORF Names:CG1004
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0004635. rho.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 98CytoplasmicSequence analysisAdd BLAST98
Transmembranei99 – 119HelicalSequence analysisAdd BLAST21
Topological domaini120 – 162LumenalSequence analysisAdd BLAST43
Transmembranei163 – 183HelicalSequence analysisAdd BLAST21
Topological domaini184 – 188CytoplasmicSequence analysis5
Transmembranei189 – 209HelicalSequence analysisAdd BLAST21
Topological domaini210LumenalSequence analysis1
Transmembranei211 – 231HelicalSequence analysisAdd BLAST21
Topological domaini232 – 244CytoplasmicSequence analysisAdd BLAST13
Transmembranei245 – 265HelicalSequence analysisAdd BLAST21
Topological domaini266 – 275LumenalSequence analysis10
Transmembranei276 – 296HelicalSequence analysisAdd BLAST21
Topological domaini297 – 308CytoplasmicSequence analysisAdd BLAST12
Transmembranei309 – 329HelicalSequence analysisAdd BLAST21
Topological domaini330 – 355LumenalSequence analysisAdd BLAST26

GO - Cellular componenti

  • apical part of cell Source: FlyBase
  • Golgi apparatus Source: FlyBase
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: FlyBase
  • mitochondrial inner membrane Source: GO_Central
  • plasma membrane Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi151W → A: Abolishes protease activity. 1 Publication1
Mutagenesisi152R → A: Abolishes protease activity. 1 Publication1
Mutagenesisi169N → A: Abolishes protease activity. 1 Publication1
Mutagenesisi215G → A: Abolishes protease activity. 1 Publication1
Mutagenesisi216A → P: Abolishes protease activity. 1 Publication1
Mutagenesisi217S → A, C or T: Abolishes protease activity. 2 Publications1
Mutagenesisi281H → A: Abolishes protease activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002061791 – 355Protein rhomboidAdd BLAST355

Proteomic databases

PaxDbiP20350.
PRIDEiP20350.

Expressioni

Developmental stagei

Early blastoderm stages and later during nervous development.

Gene expression databases

BgeeiFBgn0004635.
ExpressionAtlasiP20350. baseline.
GenevisibleiP20350. DM.

Interactioni

Protein-protein interaction databases

BioGridi63708. 27 interactors.
IntActiP20350. 1 interactor.
MINTiMINT-4508920.
STRINGi7227.FBpp0305409.

Structurei

3D structure databases

ProteinModelPortaliP20350.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S54 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2289. Eukaryota.
COG0705. LUCA.
InParanoidiP20350.
KOiK02857.
OMAiFGHREYE.
OrthoDBiEOG091G12OK.
PhylomeDBiP20350.

Family and domain databases

Gene3Di1.20.1540.10. 1 hit.
InterProiIPR002610. Peptidase_S54_rhomboid.
IPR022764. Peptidase_S54_rhomboid_dom.
IPR017213. Peptidase_S54_rhomboid_met.
[Graphical view]
PANTHERiPTHR22936. PTHR22936. 1 hit.
PfamiPF01694. Rhomboid. 1 hit.
[Graphical view]
PIRSFiPIRSF037470. Rhomboid. 1 hit.

Sequencei

Sequence statusi: Complete.

P20350-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENLTQNVNE TKVDLGQEKE KEASQEEEHA TAAKETIIDI PAACSSSSNS
60 70 80 90 100
SSYDTDCSTA SSTCCTRQGE HIYMQREAIP ATPLPESEDI GLLKYVHRQH
110 120 130 140 150
WPWFILVISI IEIAIFAYDR YTMPAQNFGL PVPIPSDSVL VYRPDRRLQV
160 170 180 190 200
WRFFSYMFLH ANWFHLGFNI VIQLFFGIPL EVMHGTARIG VIYMAGVFAG
210 220 230 240 250
SLGTSVVDSE VFLVGASGGV YALLAAHLAN ITLNYAHMKS ASTQLGSVVI
260 270 280 290 300
FVSCDLGYAL YTQYFDGSAF AKGPQVSYIA HLTGALAGLT IGFLVLKNFG
310 320 330 340 350
HREYEQLIWW LALGVYCAFT VFAIVFNLIN TVTAQLMEEQ GEVITQHLLH

DLGVS
Length:355
Mass (Da):39,330
Last modified:March 5, 2002 - v2
Checksum:i2E0572D08831C5D0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4L → P in CAA36692 (PubMed:2110920).Curated1
Sequence conflicti33A → V in CAA36692 (PubMed:2110920).Curated1
Sequence conflicti46S → T in CAA36692 (PubMed:2110920).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52454 mRNA. Translation: CAA36692.1.
AE014296 Genomic DNA. Translation: AAF47496.1.
PIRiA34597.
RefSeqiNP_001261255.1. NM_001274326.1.
NP_523883.2. NM_079159.3.
UniGeneiDm.808.

Genome annotation databases

EnsemblMetazoaiFBtr0072694; FBpp0072578; FBgn0004635.
FBtr0333207; FBpp0305409; FBgn0004635.
GeneIDi38168.
KEGGidme:Dmel_CG1004.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52454 mRNA. Translation: CAA36692.1.
AE014296 Genomic DNA. Translation: AAF47496.1.
PIRiA34597.
RefSeqiNP_001261255.1. NM_001274326.1.
NP_523883.2. NM_079159.3.
UniGeneiDm.808.

3D structure databases

ProteinModelPortaliP20350.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi63708. 27 interactors.
IntActiP20350. 1 interactor.
MINTiMINT-4508920.
STRINGi7227.FBpp0305409.

Protein family/group databases

MEROPSiS54.001.
TCDBi9.B.104.2.1. the rhomboid protease (rhomboid) family.

Proteomic databases

PaxDbiP20350.
PRIDEiP20350.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0072694; FBpp0072578; FBgn0004635.
FBtr0333207; FBpp0305409; FBgn0004635.
GeneIDi38168.
KEGGidme:Dmel_CG1004.

Organism-specific databases

CTDi6010.
FlyBaseiFBgn0004635. rho.

Phylogenomic databases

eggNOGiKOG2289. Eukaryota.
COG0705. LUCA.
InParanoidiP20350.
KOiK02857.
OMAiFGHREYE.
OrthoDBiEOG091G12OK.
PhylomeDBiP20350.

Enzyme and pathway databases

SignaLinkiP20350.

Miscellaneous databases

ChiTaRSirho. fly.
GenomeRNAii38168.
PROiP20350.

Gene expression databases

BgeeiFBgn0004635.
ExpressionAtlasiP20350. baseline.
GenevisibleiP20350. DM.

Family and domain databases

Gene3Di1.20.1540.10. 1 hit.
InterProiIPR002610. Peptidase_S54_rhomboid.
IPR022764. Peptidase_S54_rhomboid_dom.
IPR017213. Peptidase_S54_rhomboid_met.
[Graphical view]
PANTHERiPTHR22936. PTHR22936. 1 hit.
PfamiPF01694. Rhomboid. 1 hit.
[Graphical view]
PIRSFiPIRSF037470. Rhomboid. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRHOM_DROME
AccessioniPrimary (citable) accession number: P20350
Secondary accession number(s): Q9W0F2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: March 5, 2002
Last modified: November 30, 2016
This is version 155 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.