P20348 (JANA_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sex-regulated protein janus-A

Gene names

Name:	janA
ORF Names:	CG7933

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	135 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	JanA and janB regulate somatic sex differentiation. Ref.1
Tissue specificity	Somatic and germline cells. Isoform B is expressed in both sexes and in somatic and germ line cells. Isoform A is expressed in males and is germ line specific. Ref.1
Developmental stage	The non-sex-specific transcript is present at all stages. Ref.1
Miscellaneous	Transcription of janA gives rise to two sex-specific and one non-sex-specific transcripts.
Sequence similarities	Belongs to the janus family.

Ontologies

Keywords
Biological process	Differentiation Sexual differentiation
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW sex differentiation Inferred from expression pattern Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform B (identifier: P20348-1)

Also known as: jan2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A (identifier: P20348-2)

Also known as: C; jan1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-16: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 135	135	Sex-regulated protein janus-A		PRO_0000206158
Regions
	Region	104 – 106	3	Substrate binding By similarity
Sites
	Active site	63	1	Proton acceptor By similarity
	Binding site	37	1	Substrate By similarity
Natural variations
	Alternative sequence	1 – 16	16	Missing in isoform A.		VSP_009384
Experimental info
	Sequence conflict	44	1	F → I in AAL90058. Ref.4
	Sequence conflict	58	1	A → T in AAL90058. Ref.4
	Sequence conflict	94	1	E → D in AAL90058. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform B (jan2) [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 2720237CE77F3132	FASTA	135	15,220
	10 20 30 40 50 60 MNRLQLLSKG LRLIHKMSEE ALAGVPLVHI SPEGIFKYVM INVFDGGDAS KAVIRGFADC 70 80 90 100 110 120 TWHADIFERE EEVFKKLGLR AECPGGGRIE HNPEKKYLKV YGYSQGFGKA DHAQTKRILA 130 TKYPDYTIEI SDEGY
	Isoform A (C) (jan1) [UniParc]. Checksum: 2DE4F54603006196	FASTA	119	13,318
	10 20 30 40 50 60 MSEEALAGVP LVHISPEGIF KYVMINVFDG GDASKAVIRG FADCTWHADI FEREEEVFKK 70 80 90 100 110 LGLRAECPGG GRIEHNPEKK YLKVYGYSQG FGKADHAQTK RILATKYPDY TIEISDEGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Transcriptional and posttranscriptional regulation contributes to the sex-regulated expression of two sequence-related genes at the janus locus of Drosophila melanogaster." Yanicostas C., Vincent A., Lepesant J.-A. Mol. Cell. Biol. 9:2526-2535(1989) [PubMed: 2503707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS A AND B), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: Canton-S.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M27033 Genomic DNA. Translation: AAC34203.1. M27033 Genomic DNA. Translation: AAC34202.1. AE014297 Genomic DNA. Translation: AAN14209.1. AE014297 Genomic DNA. Translation: AAO41613.1. AE014297 Genomic DNA. Translation: AAS65231.1. AY089320 mRNA. Translation: AAL90058.1.
PIR	A32317.
RefSeq	NP_788762.1. NM_176585.1. NP_788763.1. NM_176586.3. NP_996311.1. NM_206588.1.
UniGene	Dm.12685.
3D structure databases
ProteinModelPortal	P20348.
SMR	P20348. Positions 17-135.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-18771N.
MINT	MINT-954907.
STRING	P20348.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0085596; FBpp0084962; FBgn0001280.
GeneID	43569.
KEGG	dme:Dmel_CG7933.
Organism-specific databases
CTD	43569.
FlyBase	FBgn0001280. janA.
Phylogenomic databases
eggNOG	inNOG11111.
GeneTree	EMGT00050000017891.
InParanoid	P20348.
OMA	VYGYSMG.
OrthoDB	EOG48KPV3.
PhylomeDB	P20348.
Gene expression databases
Bgee	P20348.
GermOnline	CG7933. Drosophila melanogaster.
Family and domain databases
InterPro	IPR007702. Ocnus. [Graphical view]
Pfam	PF05005. Ocnus. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	834603.

Entry information

Entry name

JANA_DROME

Accession

Primary (citable) accession number: P20348
Secondary accession number(s): A4V3M6, Q8T475, Q9VAB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project