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Protein

Ras-related protein Rab-6A

Gene

RAB6A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Has a low GTPase activity.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 28GTP3 Publications9
Nucleotide bindingi68 – 72GTP3 Publications5
Nucleotide bindingi126 – 129GTP3 Publications4
Nucleotide bindingi156 – 158GTP3 Publications3

GO - Molecular functioni

  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB
  • myosin V binding Source: UniProtKB
  • protein domain specific binding Source: BHF-UCL

GO - Biological processi

  • antigen processing and presentation Source: UniProtKB
  • early endosome to Golgi transport Source: UniProtKB
  • minus-end-directed organelle transport along microtubule Source: BHF-UCL
  • peptidyl-cysteine methylation Source: MGI
  • protein localization to Golgi apparatus Source: BHF-UCL
  • protein targeting to Golgi Source: UniProtKB
  • retrograde vesicle-mediated transport, Golgi to ER Source: BHF-UCL
  • small GTPase mediated signal transduction Source: InterPro
  • viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Host-virus interaction, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000175582-MONOMER.
ReactomeiR-HSA-1912420. Pre-NOTCH Processing in Golgi.
R-HSA-6798695. Neutrophil degranulation.
R-HSA-6811436. COPI-independent Golgi-to-ER retrograde traffic.
R-HSA-6811440. Retrograde transport at the Trans-Golgi-Network.
R-HSA-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-6A
Short name:
Rab-6
Gene namesi
Name:RAB6A
Synonyms:RAB6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:9786. RAB6A.

Subcellular locationi

Isoform 1 :
Isoform 2 :

GO - Cellular componenti

  • cytoplasmic vesicle Source: BHF-UCL
  • cytosol Source: BHF-UCL
  • endoplasmic reticulum membrane Source: Reactome
  • endosome to plasma membrane transport vesicle Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • Golgi membrane Source: Reactome
  • membrane Source: BHF-UCL
  • trans-Golgi network Source: BHF-UCL
  • trans-Golgi network membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi27T → N: Loss of APBA1-binding. No loss of RIC1- and RGP1-binding. 2 Publications1
Mutagenesisi46I → E: Loss of RAB6IP1-binding. 1 Publication1
Mutagenesisi72Q → L: Loss of GTPase activity. Interacts with APBA1. 4 Publications1

Organism-specific databases

DisGeNETi5870.
OpenTargetsiENSG00000175582.
PharmGKBiPA34146.

Polymorphism and mutation databases

BioMutaiRAB6A.
DMDMi131796.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001211122 – 208Ras-related protein Rab-6AAdd BLAST207

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1 Publication1
Modified residuei82O-AMP-tyrosine; by Legionella DrrA1 Publication1
Modified residuei184PhosphoserineBy similarity1
Lipidationi206S-geranylgeranyl cysteineBy similarity1
Modified residuei208Cysteine methyl esterBy similarity1
Lipidationi208S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

EPDiP20340.
MaxQBiP20340.
PaxDbiP20340.
PeptideAtlasiP20340.
PRIDEiP20340.
TopDownProteomicsiP20340-2. [P20340-2]

PTM databases

iPTMnetiP20340.
PhosphoSitePlusiP20340.
SwissPalmiP20340.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSG00000175582.
CleanExiHS_RAB6A.
ExpressionAtlasiP20340. baseline and differential.
GenevisibleiP20340. HS.

Organism-specific databases

HPAiCAB009936.
HPA059131.

Interactioni

Subunit structurei

Interacts with BICDL1; leads to its accumulation in the pericentrosomal region (By similarity). Interacts with SCYL1BP1. Interacts with VSP52 and RABGAP1. Interacts with GCC2 (via its GRIP domain). Interacts with RAB6IP1 (via its RUN 1 domain). Isoform 1 interacts with RAB6KIFL. Isoform 2 does not interact with RAB6KIFL. Isoform 1 interacts with BICD1. Isoform 2 interacts with BICD1. Isoform 1 interacts with BICD2. Isoform 2 interacts with BICD2. Interacts with TMF1. Isoform 1 (GTP-bound) interacts with DYNLRB1; the interaction is direct. Isoform 2 (GDP-bound) interacts with DYNLRB1; the interaction is direct. Interacts with PIFO. Interacts (GTP-bound) with APBA1/MINT1 isoform 2, also called Mint1_826, but not with isoform 1. Interacts with RIC1 and RGP1; the interactions are direct with a preference for RAB6A-GDP. Interacts with human cytomegalovirus protein UL32 (PubMed:21411515).By similarity15 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
APBA1Q02410-24EBI-8851226,EBI-9247455
GCC2Q8IWJ24EBI-1052826,EBI-1645320
OCRLQ0196811EBI-1052826,EBI-6148898
PMM1Q928714EBI-1052826,EBI-746784

GO - Molecular functioni

  • myosin V binding Source: UniProtKB
  • protein domain specific binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi111808. 40 interactors.
DIPiDIP-39668N.
IntActiP20340. 16 interactors.
MINTiMINT-1482056.
STRINGi9606.ENSP00000311449.

Structurei

Secondary structure

1208
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi15 – 21Combined sources7
Helixi26 – 35Combined sources10
Beta strandi47 – 56Combined sources10
Beta strandi61 – 69Combined sources9
Helixi73 – 78Combined sources6
Helixi79 – 83Combined sources5
Beta strandi87 – 94Combined sources8
Helixi98 – 102Combined sources5
Helixi104 – 115Combined sources12
Beta strandi118 – 126Combined sources9
Helixi128 – 130Combined sources3
Helixi131 – 133Combined sources3
Helixi138 – 147Combined sources10
Beta strandi151 – 154Combined sources4
Turni157 – 159Combined sources3
Helixi163 – 173Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YZQX-ray1.78A10-177[»]
2GILX-ray1.82A/B/C/D13-174[»]
3BBPX-ray3.00A/B/C1-206[»]
3CWZX-ray3.20A8-195[»]
4DKXX-ray1.90A/B1-208[»]
ProteinModelPortaliP20340.
SMRiP20340.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20340.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi42 – 50Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0094. Eukaryota.
ENOG410XPBI. LUCA.
GeneTreeiENSGT00730000110423.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP20340.
KOiK07893.
OMAiLSTTKWI.
OrthoDBiEOG091G0ORZ.
PhylomeDBiP20340.
TreeFamiTF300803.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P20340-1) [UniParc]FASTAAdd to basket
Also known as: Rab-6a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTGGDFGNP LRKFKLVFLG EQSVGKTSLI TRFMYDSFDN TYQATIGIDF
60 70 80 90 100
LSKTMYLEDR TVRLQLWDTA GQERFRSLIP SYIRDSTVAV VVYDITNVNS
110 120 130 140 150
FQQTTKWIDD VRTERGSDVI IMLVGNKTDL ADKRQVSIEE GERKAKELNV
160 170 180 190 200
MFIETSAKAG YNVKQLFRRV AAALPGMEST QDRSREDMID IKLEKPQEQP

VSEGGCSC
Length:208
Mass (Da):23,593
Last modified:January 23, 2007 - v3
Checksum:iC9A3C2DDBF6C3E9E
GO
Isoform 2 (identifier: P20340-2) [UniParc]FASTAAdd to basket
Also known as: Rab-6a', Rab-6C, Rab6C

The sequence of this isoform differs from the canonical sequence as follows:
     62-88: VRLQLWDTAGQERFRSLIPSYIRDSTV → IRLQLWDTAGQERFRSLIPSYIRDSAA

Show »
Length:208
Mass (Da):23,549
Checksum:iDF4B42559600F98B
GO
Isoform 3 (identifier: P20340-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     62-165: Missing.

Show »
Length:104
Mass (Da):11,715
Checksum:i47907D182528BE16
GO
Isoform 4 (identifier: P20340-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: Missing.
     62-88: VRLQLWDTAGQERFRSLIPSYIRDSTV → IRLQLWDTAGQERFRSLIPSYIRDSAA

Show »
Length:175
Mass (Da):19,935
Checksum:i1667A3BFAE94F873
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti189I → T in BAH13508 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0469671 – 33Missing in isoform 4. 1 PublicationAdd BLAST33
Alternative sequenceiVSP_04530262 – 165Missing in isoform 3. 1 PublicationAdd BLAST104
Alternative sequenceiVSP_00552762 – 88VRLQL…RDSTV → IRLQLWDTAGQERFRSLIPS YIRDSAA in isoform 2 and isoform 4. 6 PublicationsAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28212 mRNA. Translation: AAA60246.1.
AF130986 mRNA. Translation: AAD25535.1.
AF119836 mRNA. Translation: AAF23593.1.
AF198616 mRNA. Translation: AAF73841.1.
AF130122 mRNA. Translation: AAD27707.1.
DQ437503 mRNA. Translation: ABD93919.1.
AK057157 mRNA. Translation: BAB71371.1.
AK289748 mRNA. Translation: BAF82437.1.
AK290132 mRNA. Translation: BAF82821.1.
AK301534 mRNA. Translation: BAH13508.1.
AF498939 mRNA. Translation: AAM21087.1.
AF498941 mRNA. Translation: AAM21089.1.
BT019698 mRNA. Translation: AAV38504.1.
AP002770 Genomic DNA. No translation available.
AP002993 Genomic DNA. No translation available.
BC003617 mRNA. Translation: AAH03617.1.
BC068486 mRNA. Translation: AAH68486.1.
BC096818 mRNA. Translation: AAH96818.1.
CCDSiCCDS58155.1. [P20340-4]
CCDS58156.1. [P20340-3]
CCDS8223.1. [P20340-2]
CCDS8224.1. [P20340-1]
PIRiG34323.
RefSeqiNP_001230647.1. NM_001243718.1. [P20340-3]
NP_001230648.1. NM_001243719.1. [P20340-4]
NP_002860.2. NM_002869.4. [P20340-2]
NP_942599.1. NM_198896.1. [P20340-1]
UniGeneiHs.503222.
Hs.591552.

Genome annotation databases

EnsembliENST00000310653; ENSP00000311449; ENSG00000175582. [P20340-2]
ENST00000336083; ENSP00000336850; ENSG00000175582. [P20340-1]
ENST00000541588; ENSP00000445350; ENSG00000175582. [P20340-3]
ENST00000541973; ENSP00000443782; ENSG00000175582. [P20340-4]
GeneIDi5870.
KEGGihsa:5870.
UCSCiuc001oue.4. human. [P20340-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28212 mRNA. Translation: AAA60246.1.
AF130986 mRNA. Translation: AAD25535.1.
AF119836 mRNA. Translation: AAF23593.1.
AF198616 mRNA. Translation: AAF73841.1.
AF130122 mRNA. Translation: AAD27707.1.
DQ437503 mRNA. Translation: ABD93919.1.
AK057157 mRNA. Translation: BAB71371.1.
AK289748 mRNA. Translation: BAF82437.1.
AK290132 mRNA. Translation: BAF82821.1.
AK301534 mRNA. Translation: BAH13508.1.
AF498939 mRNA. Translation: AAM21087.1.
AF498941 mRNA. Translation: AAM21089.1.
BT019698 mRNA. Translation: AAV38504.1.
AP002770 Genomic DNA. No translation available.
AP002993 Genomic DNA. No translation available.
BC003617 mRNA. Translation: AAH03617.1.
BC068486 mRNA. Translation: AAH68486.1.
BC096818 mRNA. Translation: AAH96818.1.
CCDSiCCDS58155.1. [P20340-4]
CCDS58156.1. [P20340-3]
CCDS8223.1. [P20340-2]
CCDS8224.1. [P20340-1]
PIRiG34323.
RefSeqiNP_001230647.1. NM_001243718.1. [P20340-3]
NP_001230648.1. NM_001243719.1. [P20340-4]
NP_002860.2. NM_002869.4. [P20340-2]
NP_942599.1. NM_198896.1. [P20340-1]
UniGeneiHs.503222.
Hs.591552.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YZQX-ray1.78A10-177[»]
2GILX-ray1.82A/B/C/D13-174[»]
3BBPX-ray3.00A/B/C1-206[»]
3CWZX-ray3.20A8-195[»]
4DKXX-ray1.90A/B1-208[»]
ProteinModelPortaliP20340.
SMRiP20340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111808. 40 interactors.
DIPiDIP-39668N.
IntActiP20340. 16 interactors.
MINTiMINT-1482056.
STRINGi9606.ENSP00000311449.

PTM databases

iPTMnetiP20340.
PhosphoSitePlusiP20340.
SwissPalmiP20340.

Polymorphism and mutation databases

BioMutaiRAB6A.
DMDMi131796.

Proteomic databases

EPDiP20340.
MaxQBiP20340.
PaxDbiP20340.
PeptideAtlasiP20340.
PRIDEiP20340.
TopDownProteomicsiP20340-2. [P20340-2]

Protocols and materials databases

DNASUi5870.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000310653; ENSP00000311449; ENSG00000175582. [P20340-2]
ENST00000336083; ENSP00000336850; ENSG00000175582. [P20340-1]
ENST00000541588; ENSP00000445350; ENSG00000175582. [P20340-3]
ENST00000541973; ENSP00000443782; ENSG00000175582. [P20340-4]
GeneIDi5870.
KEGGihsa:5870.
UCSCiuc001oue.4. human. [P20340-1]

Organism-specific databases

CTDi5870.
DisGeNETi5870.
GeneCardsiRAB6A.
HGNCiHGNC:9786. RAB6A.
HPAiCAB009936.
HPA059131.
MIMi179513. gene.
neXtProtiNX_P20340.
OpenTargetsiENSG00000175582.
PharmGKBiPA34146.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0094. Eukaryota.
ENOG410XPBI. LUCA.
GeneTreeiENSGT00730000110423.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP20340.
KOiK07893.
OMAiLSTTKWI.
OrthoDBiEOG091G0ORZ.
PhylomeDBiP20340.
TreeFamiTF300803.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000175582-MONOMER.
ReactomeiR-HSA-1912420. Pre-NOTCH Processing in Golgi.
R-HSA-6798695. Neutrophil degranulation.
R-HSA-6811436. COPI-independent Golgi-to-ER retrograde traffic.
R-HSA-6811440. Retrograde transport at the Trans-Golgi-Network.
R-HSA-8876198. RAB GEFs exchange GTP for GDP on RABs.

Miscellaneous databases

ChiTaRSiRAB6A. human.
EvolutionaryTraceiP20340.
GeneWikiiRAB6A.
GenomeRNAii5870.
PROiP20340.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000175582.
CleanExiHS_RAB6A.
ExpressionAtlasiP20340. baseline and differential.
GenevisibleiP20340. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAB6A_HUMAN
AccessioniPrimary (citable) accession number: P20340
Secondary accession number(s): A8K133
, B7Z772, F5H668, Q1W5D8, Q5U0A8, Q9UBE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 195 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.