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Protein

Ras-related protein Rab-6A

Gene

RAB6A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Has a low GTPase activity. Involved in COPI-independent retrograde transport from the Golgi to the ER (PubMed:25962623).1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 28GTP3 Publications9
Nucleotide bindingi68 – 72GTP3 Publications5
Nucleotide bindingi126 – 129GTP3 Publications4
Nucleotide bindingi156 – 158GTP3 Publications3

GO - Molecular functioni

  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB
  • myosin V binding Source: UniProtKB
  • protein domain specific binding Source: BHF-UCL

GO - Biological processi

  • antigen processing and presentation Source: UniProtKB
  • early endosome to Golgi transport Source: UniProtKB
  • minus-end-directed organelle transport along microtubule Source: BHF-UCL
  • neutrophil degranulation Source: Reactome
  • peptidyl-cysteine methylation Source: MGI
  • protein localization to Golgi apparatus Source: UniProtKB
  • protein transport Source: UniProtKB-KW
  • retrograde vesicle-mediated transport, Golgi to ER Source: UniProtKB
  • viral process Source: UniProtKB-KW

Keywordsi

Biological processER-Golgi transport, Host-virus interaction, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-1912420 Pre-NOTCH Processing in Golgi
R-HSA-6798695 Neutrophil degranulation
R-HSA-6811436 COPI-independent Golgi-to-ER retrograde traffic
R-HSA-6811440 Retrograde transport at the Trans-Golgi-Network
R-HSA-8854214 TBC/RABGAPs
R-HSA-8873719 RAB geranylgeranylation
R-HSA-8876198 RAB GEFs exchange GTP for GDP on RABs

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-6A
Short name:
Rab-6
Gene namesi
Name:RAB6A
Synonyms:RAB6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000175582.19
HGNCiHGNC:9786 RAB6A
MIMi179513 gene
neXtProtiNX_P20340

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi27T → N: Loss of APBA1-binding. No loss of RIC1- and RGP1-binding. 2 Publications1
Mutagenesisi46I → E: Loss of RAB6IP1-binding. 1 Publication1
Mutagenesisi72Q → L: Loss of GTPase activity. Interacts with APBA1. 4 Publications1

Organism-specific databases

DisGeNETi5870
OpenTargetsiENSG00000175582
PharmGKBiPA34146

Polymorphism and mutation databases

BioMutaiRAB6A
DMDMi131796

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001211122 – 208Ras-related protein Rab-6AAdd BLAST207

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1 Publication1
Modified residuei82O-AMP-tyrosine; by Legionella DrrA1 Publication1
Modified residuei184PhosphoserineBy similarity1
Lipidationi206S-geranylgeranyl cysteineBy similarity1
Modified residuei208Cysteine methyl esterBy similarity1
Lipidationi208S-geranylgeranyl cysteineBy similarity1

Post-translational modificationi

Prenylated.1 Publication

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

EPDiP20340
MaxQBiP20340
PaxDbiP20340
PeptideAtlasiP20340
PRIDEiP20340
TopDownProteomicsiP20340-2 [P20340-2]

PTM databases

iPTMnetiP20340
PhosphoSitePlusiP20340
SwissPalmiP20340

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSG00000175582
CleanExiHS_RAB6A
ExpressionAtlasiP20340 baseline and differential
GenevisibleiP20340 HS

Organism-specific databases

HPAiCAB009936
HPA059131

Interactioni

Subunit structurei

Interacts with BICDL1; leads to its accumulation in the pericentrosomal region (By similarity). Interacts with SCYL1BP1. Interacts with VSP52 and RABGAP1. Interacts with GCC2 (via its GRIP domain). Interacts with RAB6IP1 (via its RUN 1 domain). Isoform 1 interacts with RAB6KIFL. Isoform 2 does not interact with RAB6KIFL. Isoform 1 interacts with BICD1. Isoform 2 interacts with BICD1. Isoform 1 interacts with BICD2. Isoform 2 interacts with BICD2. Interacts with TMF1. Isoform 1 (GTP-bound) interacts with DYNLRB1; the interaction is direct. Isoform 2 (GDP-bound) interacts with DYNLRB1; the interaction is direct. Interacts with PIFO. Interacts (GTP-bound) with APBA1/MINT1 isoform 2, also called Mint1_826, but not with isoform 1. Interacts with RIC1 and RGP1; the interactions are direct with a preference for RAB6A-GDP. Interacts with human cytomegalovirus protein UL32 (PubMed:21411515).By similarity17 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • myosin V binding Source: UniProtKB
  • protein domain specific binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi111808, 43 interactors
DIPiDIP-39668N
IntActiP20340, 21 interactors
MINTiP20340
STRINGi9606.ENSP00000311449

Structurei

Secondary structure

1208
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi15 – 21Combined sources7
Helixi26 – 35Combined sources10
Beta strandi47 – 56Combined sources10
Beta strandi61 – 69Combined sources9
Helixi73 – 78Combined sources6
Helixi79 – 83Combined sources5
Beta strandi87 – 94Combined sources8
Helixi98 – 102Combined sources5
Helixi104 – 115Combined sources12
Beta strandi118 – 126Combined sources9
Helixi128 – 130Combined sources3
Helixi131 – 133Combined sources3
Helixi138 – 147Combined sources10
Beta strandi151 – 154Combined sources4
Turni157 – 159Combined sources3
Helixi163 – 173Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YZQX-ray1.78A10-177[»]
2GILX-ray1.82A/B/C/D13-174[»]
3BBPX-ray3.00A/B/C1-206[»]
3CWZX-ray3.20A8-195[»]
4DKXX-ray1.90A/B1-208[»]
5LEFX-ray2.09A/B8-195[»]
ProteinModelPortaliP20340
SMRiP20340
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20340

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi42 – 50Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0094 Eukaryota
ENOG410XPBI LUCA
GeneTreeiENSGT00910000144073
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiP20340
KOiK07893
OMAiMYQATIG
OrthoDBiEOG091G0ORZ
PhylomeDBiP20340
TreeFamiTF300803

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P20340-1) [UniParc]FASTAAdd to basket
Also known as: Rab-6a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTGGDFGNP LRKFKLVFLG EQSVGKTSLI TRFMYDSFDN TYQATIGIDF
60 70 80 90 100
LSKTMYLEDR TVRLQLWDTA GQERFRSLIP SYIRDSTVAV VVYDITNVNS
110 120 130 140 150
FQQTTKWIDD VRTERGSDVI IMLVGNKTDL ADKRQVSIEE GERKAKELNV
160 170 180 190 200
MFIETSAKAG YNVKQLFRRV AAALPGMEST QDRSREDMID IKLEKPQEQP

VSEGGCSC
Length:208
Mass (Da):23,593
Last modified:January 23, 2007 - v3
Checksum:iC9A3C2DDBF6C3E9E
GO
Isoform 2 (identifier: P20340-2) [UniParc]FASTAAdd to basket
Also known as: Rab-6a', Rab-6C, Rab6C

The sequence of this isoform differs from the canonical sequence as follows:
     62-88: VRLQLWDTAGQERFRSLIPSYIRDSTV → IRLQLWDTAGQERFRSLIPSYIRDSAA

Show »
Length:208
Mass (Da):23,549
Checksum:iDF4B42559600F98B
GO
Isoform 3 (identifier: P20340-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     62-165: Missing.

Show »
Length:104
Mass (Da):11,715
Checksum:i47907D182528BE16
GO
Isoform 4 (identifier: P20340-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: Missing.
     62-88: VRLQLWDTAGQERFRSLIPSYIRDSTV → IRLQLWDTAGQERFRSLIPSYIRDSAA

Show »
Length:175
Mass (Da):19,935
Checksum:i1667A3BFAE94F873
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti189I → T in BAH13508 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0469671 – 33Missing in isoform 4. 1 PublicationAdd BLAST33
Alternative sequenceiVSP_04530262 – 165Missing in isoform 3. 1 PublicationAdd BLAST104
Alternative sequenceiVSP_00552762 – 88VRLQL…RDSTV → IRLQLWDTAGQERFRSLIPS YIRDSAA in isoform 2 and isoform 4. 6 PublicationsAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28212 mRNA Translation: AAA60246.1
AF130986 mRNA Translation: AAD25535.1
AF119836 mRNA Translation: AAF23593.1
AF198616 mRNA Translation: AAF73841.1
AF130122 mRNA Translation: AAD27707.1
DQ437503 mRNA Translation: ABD93919.1
AK057157 mRNA Translation: BAB71371.1
AK289748 mRNA Translation: BAF82437.1
AK290132 mRNA Translation: BAF82821.1
AK301534 mRNA Translation: BAH13508.1
AF498939 mRNA Translation: AAM21087.1
AF498941 mRNA Translation: AAM21089.1
BT019698 mRNA Translation: AAV38504.1
AP002770 Genomic DNA No translation available.
AP002993 Genomic DNA No translation available.
BC003617 mRNA Translation: AAH03617.1
BC068486 mRNA Translation: AAH68486.1
BC096818 mRNA Translation: AAH96818.1
CCDSiCCDS58155.1 [P20340-4]
CCDS58156.1 [P20340-3]
CCDS8223.1 [P20340-2]
CCDS8224.1 [P20340-1]
PIRiG34323
RefSeqiNP_001230647.1, NM_001243718.1 [P20340-3]
NP_001230648.1, NM_001243719.1 [P20340-4]
NP_002860.2, NM_002869.4 [P20340-2]
NP_942599.1, NM_198896.1 [P20340-1]
UniGeneiHs.503222
Hs.591552

Genome annotation databases

EnsembliENST00000310653; ENSP00000311449; ENSG00000175582 [P20340-2]
ENST00000336083; ENSP00000336850; ENSG00000175582 [P20340-1]
ENST00000541588; ENSP00000445350; ENSG00000175582 [P20340-3]
ENST00000541973; ENSP00000443782; ENSG00000175582 [P20340-4]
GeneIDi5870
KEGGihsa:5870
UCSCiuc001oue.4 human [P20340-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRAB6A_HUMAN
AccessioniPrimary (citable) accession number: P20340
Secondary accession number(s): A8K133
, B7Z772, F5H668, Q1W5D8, Q5U0A8, Q9UBE4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 208 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health