P20338 (RAB4A_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 135.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Ras-related protein Rab-4A | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 213 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Protein transport. Probably involved in vesicular traffic By similarity. |
| Subunit structure | Interacts with SGSM1, SGSM2 and SGSM3 By similarity. Interacts with RAB11FIP1, RABEP1, ZFYVE20 and RUFY1. Interacts (membrane-bound form) with NDRG1; the interaction involves NDRG1 in vesicular recycling of E-cadherin. Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.18 |
| Subcellular location | Membrane; Peripheral membrane protein. Cytoplasm. Note: Generally associated with membranes. Cytoplasmic when phosphorylated by CDK1. |
| Post-translational modification | |
| Sequence similarities | Belongs to the small GTPase superfamily. Rab family. |
| Sequence caution | The sequence AAH02438.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAH04309.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAM21082.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAT91347.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAG36543.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence CAI19037.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence CAI22870.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence EAW69890.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Protein transport Transport |
| Cellular component | Cytoplasm Membrane |
| Ligand | GTP-binding Nucleotide-binding |
| PTM | Lipoprotein Methylation Phosphoprotein Prenylation |
| Technical term | 3D-structure Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | protein transport Inferred from electronic annotation. Source: UniProtKB-KW small GTPase mediated signal transductionInferred from electronic annotation. Source: InterPro |
| Molecular function | GDP binding Inferred from direct assay Ref.17Ref.18. Source: UniProtKB GTP bindingInferred from direct assay Ref.17Ref.18. Source: UniProtKB GTPase activityInferred from direct assay Ref.17Ref.18. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||||||||||||||||||||||||
Molecule processing | |||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 213 | 213 | Ras-related protein Rab-4A | PRO_0000121093 | |||||||||||||||||||||||||||||||||||
Regions | |||||||||||||||||||||||||||||||||||||||
| Nucleotide binding | 15 – 23 | 9 | GTP | ||||||||||||||||||||||||||||||||||||
| Nucleotide binding | 63 – 67 | 5 | GTP | ||||||||||||||||||||||||||||||||||||
| Nucleotide binding | 121 – 124 | 4 | GTP | ||||||||||||||||||||||||||||||||||||
| Nucleotide binding | 151 – 153 | 3 | GTP | ||||||||||||||||||||||||||||||||||||
| Motif | 37 – 45 | 9 | Effector region By similarity | ||||||||||||||||||||||||||||||||||||
Amino acid modifications | |||||||||||||||||||||||||||||||||||||||
| Modified residue | 199 | 1 | Phosphoserine; by CDK1 | ||||||||||||||||||||||||||||||||||||
| Modified residue | 213 | 1 | Cysteine methyl ester By similarity | ||||||||||||||||||||||||||||||||||||
| Lipidation | 211 | 1 | S-geranylgeranyl cysteine By similarity | ||||||||||||||||||||||||||||||||||||
| Lipidation | 213 | 1 | S-geranylgeranyl cysteine By similarity | ||||||||||||||||||||||||||||||||||||
Experimental info | |||||||||||||||||||||||||||||||||||||||
| Mutagenesis | 46 | 1 | G → A: 10-fold decrease in ZFYVE20 binding affinity. Ref.18 | ||||||||||||||||||||||||||||||||||||
| Mutagenesis | 46 | 1 | G → L: 10-fold decrease in ZFYVE20 binding affinity. Ref.18 | ||||||||||||||||||||||||||||||||||||
| Sequence conflict | 157 | 1 | N → D in AAA60244. Ref.1 | ||||||||||||||||||||||||||||||||||||
| Sequence conflict | 203 | 1 | A → T in AAA60244. Ref.1 | ||||||||||||||||||||||||||||||||||||
Secondary structure | |||||||||||||||||||||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||||||||||||||||||||
| Beta strand | 6 – 16 | 11 | |||||||||||||||||||||||||||||||||||||
| Helix | 21 – 30 | 10 | |||||||||||||||||||||||||||||||||||||
| Beta strand | 41 – 52 | 12 | |||||||||||||||||||||||||||||||||||||
| Beta strand | 55 – 64 | 10 | |||||||||||||||||||||||||||||||||||||
| Helix | 68 – 70 | 3 | |||||||||||||||||||||||||||||||||||||
| Helix | 71 – 74 | 4 | |||||||||||||||||||||||||||||||||||||
| Helix | 75 – 78 | 4 | |||||||||||||||||||||||||||||||||||||
| Beta strand | 83 – 89 | 7 | |||||||||||||||||||||||||||||||||||||
| Helix | 93 – 97 | 5 | |||||||||||||||||||||||||||||||||||||
| Helix | 99 – 109 | 11 | |||||||||||||||||||||||||||||||||||||
| Beta strand | 115 – 121 | 7 | |||||||||||||||||||||||||||||||||||||
| Helix | 126 – 128 | 3 | |||||||||||||||||||||||||||||||||||||
| Helix | 133 – 142 | 10 | |||||||||||||||||||||||||||||||||||||
| Beta strand | 146 – 149 | 4 | |||||||||||||||||||||||||||||||||||||
| Turn | 152 – 154 | 3 | |||||||||||||||||||||||||||||||||||||
| Helix | 158 – 170 | 13 | |||||||||||||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion." Zahraoui A., Touchot N., Chardin P., Tavitian A. J. Biol. Chem. 264:12394-12401(1989) [PubMed: 2501306] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Regulation of CD4 expression via recycling by HRES-1/RAB4 controls susceptibility to HIV infection." Nagy G., Ward J., Mosser D.D., Koncz A., Gergely P. Jr., Stancato C., Qian Y., Fernandez D., Niland B., Grossman C.E., Telarico T., Banki K., Perl A. J. Biol. Chem. 281:34574-34591(2006) [PubMed: 16935861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain. |
| [4] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Caudate nucleus. |
| [5] | "The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. Bentley D.R.Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [6] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [7] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin and Uterus. |
| [8] | "Phosphorylation of two small GTP-binding proteins of the Rab family by p34cdc2." Bailly E., McCaffrey M., Touchot N., Zahraoui A., Goud B., Bornens M. Nature 350:715-718(1991) [PubMed: 1902553] [Abstract] Cited for: PHOSPHORYLATION BY CDK1. |
| [9] | "Reversible phosphorylation-dephosphorylation determines the localization of rab4 during the cell cycle." van der Sluijs P., Hull M., Huber L.A., Male P., Goud B., Mellman I. EMBO J. 11:4379-4389(1992) [PubMed: 1425574] [Abstract] Cited for: PHOSPHORYLATION BY CDK1. |
| [10] | "Rabaptin4, a novel effector of the samll GTPase rab4a, is recruited to perinuclear recycling vesicles." Nagelkerken B., van Anken E., van Raak M., Gerez L., Mohrmann K., van Uden N., Holthuizen J., Pelkmans L., van der Sluijs P. Biochem. J. 346:593-601(2000) [PubMed: 10698684] [Abstract] Cited for: INTERACTION WITH RABEP1. |
| [11] | "Rab coupling protein (RCP), a novel Rab4 and Rab11 effector protein." Lindsay A.J., Hendrick A.G., Cantalupo G., Senic-Matuglia F., Goud B., Bucci C., McCaffrey M.W. J. Biol. Chem. 277:12190-12199(2002) [PubMed: 11786538] [Abstract] Cited for: INTERACTION WITH RAB11FIP1. Tissue: Cervix carcinoma. |
| [12] | "Divalent Rab effectors regulate the sub-compartmental organization and sorting of early endosomes." de Renzis S., Soennichsen B., Zerial M. Nat. Cell Biol. 4:124-133(2002) [PubMed: 11788822] [Abstract] Cited for: INTERACTION WITH ZFYVE20. |
| [13] | "Characterisation of the Rab binding properties of Rab coupling protein (RCP) by site-directed mutagenesis." Lindsay A.J., McCaffrey M.W. FEBS Lett. 571:86-92(2004) [PubMed: 15280022] [Abstract] Cited for: INTERACTION WITH RAB11FIP1. |
| [14] | "Rabip4' is an effector of rab5 and rab4 and regulates transport through early endosomes." Fouraux M.A., Deneka M., Ivan V., van der Heijden A., Raymackers J., van Suylekom D., van Venrooij W.J., van der Sluijs P., Pruijn G.J.M. Mol. Biol. Cell 15:611-624(2004) [PubMed: 14617813] [Abstract] Cited for: INTERACTION WITH RUFY1. |
| [15] | "The N-Myc down regulated Gene1 (NDRG1) is a Rab4a effector involved in vesicular recycling of E-cadherin." Kachhap S.K., Faith D., Qian D.Z., Shabbeer S., Galloway N.L., Pili R., Denmeade S.R., DeMarzo A.M., Carducci M.A. PLoS ONE 2:E844-E844(2007) [PubMed: 17786215] [Abstract] Cited for: INTERACTION WITH NDRG1. |
| [16] | "Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. |
| [17] | "High resolution crystal structures of human Rab4a in its active and inactive conformations." Huber S.K., Scheidig A.J. FEBS Lett. 579:2821-2829(2005) [PubMed: 15907487] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.57 ANGSTROMS) OF 1-184 IN COMPLEX WITH GTP ANALOG AND GDP. |
| [18] | "Structural basis of family-wide Rab GTPase recognition by rabenosyn-5." Eathiraj S., Pan X., Ritacco C., Lambright D.G. Nature 436:415-419(2005) [PubMed: 16034420] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.92 ANGSTROMS) OF 2-172 IN COMPLEX WITH GTP, INTERACTION WITH ZFYVE20, MUTAGENESIS OF GLY-46. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | M28211 mRNA. Translation: AAA60244.1. AY585832 mRNA. Translation: AAT91347.1. Different initiation. AF498934 mRNA. Translation: AAM21082.1. Different initiation. AK313807 mRNA. Translation: BAG36543.1. Different initiation. AL162595, AL117350 Genomic DNA. Translation: CAI19037.1. Different initiation. AL117350, AL162595 Genomic DNA. Translation: CAI22870.1. Different initiation. CH471098 Genomic DNA. Translation: EAW69890.1. Different initiation. BC002438 mRNA. Translation: AAH02438.1. Different initiation. BC004309 mRNA. Translation: AAH04309.1. Different initiation. | ||||||||||||||||||||||||||||||
| IPI | IPI00480056. | ||||||||||||||||||||||||||||||
| PIR | E34323. | ||||||||||||||||||||||||||||||
| RefSeq | NP_004569.2. NM_004578.2. | ||||||||||||||||||||||||||||||
| UniGene | Hs.296169. | ||||||||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||||||||
| PDBe RCSB PDB PDBj |
| ||||||||||||||||||||||||||||||
| ProteinModelPortal | P20338. | ||||||||||||||||||||||||||||||
| SMR | P20338. Positions 2-177. | ||||||||||||||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||||||||||||||
| DIP | DIP-372N. | ||||||||||||||||||||||||||||||
| IntAct | P20338. 9 interactions. | ||||||||||||||||||||||||||||||
| MINT | MINT-1376149. | ||||||||||||||||||||||||||||||
| STRING | P20338. | ||||||||||||||||||||||||||||||
PTM databases | |||||||||||||||||||||||||||||||
| PhosphoSite | P20338. | ||||||||||||||||||||||||||||||
Polymorphism databases | |||||||||||||||||||||||||||||||
| DMDM | 29337213. | ||||||||||||||||||||||||||||||
Proteomic databases | |||||||||||||||||||||||||||||||
| PRIDE | P20338. | ||||||||||||||||||||||||||||||
Protocols and materials databases | |||||||||||||||||||||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||||||||
| Ensembl | ENST00000366690; ENSP00000355651; ENSG00000168118. | ||||||||||||||||||||||||||||||
| GeneID | 5867. | ||||||||||||||||||||||||||||||
| KEGG | hsa:5867. | ||||||||||||||||||||||||||||||
| UCSC | uc001hth.1. human. | ||||||||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||||||||
| CTD | 5867. | ||||||||||||||||||||||||||||||
| GeneCards | GC01P229406. | ||||||||||||||||||||||||||||||
| HGNC | HGNC:9781. RAB4A. | ||||||||||||||||||||||||||||||
| HPA | CAB004990. | ||||||||||||||||||||||||||||||
| MIM | 179511. gene. | ||||||||||||||||||||||||||||||
| neXtProt | NX_P20338. | ||||||||||||||||||||||||||||||
| PharmGKB | PA34141. | ||||||||||||||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||||||||
| eggNOG | prNOG18178. | ||||||||||||||||||||||||||||||
| HOVERGEN | HBG009351. | ||||||||||||||||||||||||||||||
| InParanoid | P20338. | ||||||||||||||||||||||||||||||
| OrthoDB | EOG4WM4VM. | ||||||||||||||||||||||||||||||
| PhylomeDB | P20338. | ||||||||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||||||||
| ArrayExpress | P20338. | ||||||||||||||||||||||||||||||
| Bgee | P20338. | ||||||||||||||||||||||||||||||
| CleanEx | HS_RAB4A. | ||||||||||||||||||||||||||||||
| Genevestigator | P20338. | ||||||||||||||||||||||||||||||
| GermOnline | ENSG00000168118. Homo sapiens. | ||||||||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||||||||
| InterPro | IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003579. Small_GTPase_Rab_type. [Graphical view] | ||||||||||||||||||||||||||||||
| KO | K07879. | ||||||||||||||||||||||||||||||
| Pfam | PF00071. Ras. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||
| PRINTS | PR00449. RASTRNSFRMNG. | ||||||||||||||||||||||||||||||
| SMART | SM00175. RAB. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||
| TIGRFAMs | TIGR00231. Small_GTP. 1 hit. | ||||||||||||||||||||||||||||||
| PROSITE | PS51419. RAB. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||||||||
Other | |||||||||||||||||||||||||||||||
| NextBio | 22786. | ||||||||||||||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||||||||||||||
Entry information
| Entry name | RAB4A_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P20338 Secondary accession number(s): Q5T7P7, Q9BQ44 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 1 Human chromosome 1: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with