Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ras-related protein Rab-4A

Gene

RAB4A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein transport. Probably involved in vesicular traffic (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 28GTP1 Publication9
Nucleotide bindingi68 – 72GTP1 Publication5
Nucleotide bindingi126 – 129GTP1 Publication4
Nucleotide bindingi156 – 158GTP1 Publication3

GO - Molecular functioni

  • ATPase activator activity Source: Ensembl
  • GDP binding Source: UniProtKB
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB
  • protein transporter activity Source: Ensembl

GO - Biological processi

  • antigen processing and presentation Source: UniProtKB
  • Rab protein signal transduction Source: Ensembl
  • regulation of endocytosis Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-1445148. Translocation of GLUT4 to the plasma membrane.
R-HSA-1660499. Synthesis of PIPs at the plasma membrane.
R-HSA-8875656. MET receptor recycling.
SignaLinkiP20338.
SIGNORiP20338.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-4A
Gene namesi
Name:RAB4A
Synonyms:RAB4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:9781. RAB4A.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Ensembl
  • endosome Source: GO_Central
  • extracellular exosome Source: UniProtKB
  • insulin-responsive compartment Source: GO_Central
  • perinuclear region of cytoplasm Source: UniProtKB
  • plasma membrane Source: Ensembl
  • recycling endosome Source: Ensembl
  • vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi51G → A: 10-fold decrease in ZFYVE20 binding affinity. 1 Publication1
Mutagenesisi51G → L: 10-fold decrease in ZFYVE20 binding affinity. 1 Publication1

Organism-specific databases

DisGeNETi5867.
OpenTargetsiENSG00000168118.
PharmGKBiPA34141.

Polymorphism and mutation databases

DMDMi460018296.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001210931 – 218Ras-related protein Rab-4AAdd BLAST218

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei190PhosphoserineCombined sources1
Modified residuei204Phosphoserine; by CDK11 Publication1
Lipidationi216S-geranylgeranyl cysteineBy similarity1
Modified residuei218Cysteine methyl esterBy similarity1
Lipidationi218S-geranylgeranyl cysteineBy similarity1

Post-translational modificationi

Phosphorylated by CDK1 kinase during mitosis.2 Publications

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

EPDiP20338.
MaxQBiP20338.
PaxDbiP20338.
PeptideAtlasiP20338.
PRIDEiP20338.

PTM databases

iPTMnetiP20338.
PhosphoSitePlusiP20338.

Expressioni

Gene expression databases

BgeeiENSG00000168118.
CleanExiHS_RAB4A.
ExpressionAtlasiP20338. baseline and differential.
GenevisibleiP20338. HS.

Organism-specific databases

HPAiCAB004990.

Interactioni

Subunit structurei

Interacts with SGSM1, SGSM2 and SGSM3 (By similarity). Interacts with RAB11FIP1, RABEP1, ZFYVE20 and RUFY1. Interacts (membrane-bound form) with NDRG1; the interaction involves NDRG1 in vesicular recycling of E-cadherin. Does not interact with HPS4 (By similarity).By similarity8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
EXOC5Q8IW243EBI-722284,EBI-10171392
GDI1P311503EBI-722284,EBI-946999
GDI2P503952EBI-722284,EBI-1049143
GRIPAP1Q4V3283EBI-722284,EBI-717919
VPS52Q8N1B43EBI-722284,EBI-2799833

Protein-protein interaction databases

BioGridi111805. 38 interactors.
DIPiDIP-372N.
IntActiP20338. 21 interactors.
MINTiMINT-1376149.
STRINGi9606.ENSP00000355651.

Structurei

Secondary structure

1218
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 21Combined sources11
Helixi26 – 35Combined sources10
Beta strandi46 – 57Combined sources12
Beta strandi60 – 69Combined sources10
Helixi73 – 75Combined sources3
Helixi76 – 80Combined sources5
Beta strandi87 – 94Combined sources8
Helixi98 – 102Combined sources5
Helixi104 – 114Combined sources11
Beta strandi120 – 126Combined sources7
Helixi128 – 133Combined sources6
Helixi138 – 147Combined sources10
Beta strandi151 – 154Combined sources4
Turni157 – 159Combined sources3
Helixi163 – 179Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YU9X-ray2.07A7-177[»]
1Z0KX-ray1.92A/C7-177[»]
2BMDX-ray1.80A6-189[»]
2BMEX-ray1.57A/B/C/D6-189[»]
ProteinModelPortaliP20338.
SMRiP20338.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20338.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi42 – 50Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0086. Eukaryota.
ENOG410XQ9X. LUCA.
GeneTreeiENSGT00760000118841.
HOVERGENiHBG009351.
InParanoidiP20338.
KOiK07879.
OMAiMVKLQIW.
OrthoDBiEOG091G0QOB.
PhylomeDBiP20338.
TreeFamiTF300032.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20338-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQTAMSETY DFLFKFLVIG NAGTGKSCLL HQFIEKKFKD DSNHTIGVEF
60 70 80 90 100
GSKIINVGGK YVKLQIWDTA GQERFRSVTR SYYRGAAGAL LVYDITSRET
110 120 130 140 150
YNALTNWLTD ARMLASQNIV IILCGNKKDL DADREVTFLE ASRFAQENEL
160 170 180 190 200
MFLETSALTG ENVEEAFVQC ARKILNKIES GELDPERMGS GIQYGDAALR
210
QLRSPRRAQA PNAQECGC
Length:218
Mass (Da):24,390
Last modified:March 6, 2013 - v3
Checksum:i983D65E1162741B0
GO

Sequence cautioni

The sequence AAA60244 differs from that shown. Reason: Frameshift at position 6.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti162N → D in AAA60244 (PubMed:2501306).Curated1
Sequence conflicti208A → T in AAA60244 (PubMed:2501306).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28211 mRNA. Translation: AAA60244.1. Frameshift.
AY585832 mRNA. Translation: AAT91347.1.
AF498934 mRNA. Translation: AAM21082.1.
AK313807 mRNA. Translation: BAG36543.1.
AL162595, AL117350 Genomic DNA. Translation: CAI19037.1.
AL117350, AL162595 Genomic DNA. Translation: CAI22870.1.
CH471098 Genomic DNA. Translation: EAW69890.1.
BC002438 mRNA. Translation: AAH02438.1.
BC004309 mRNA. Translation: AAH04309.1.
CCDSiCCDS31050.1.
PIRiE34323.
RefSeqiNP_001258927.1. NM_001271998.1.
NP_004569.2. NM_004578.3.
UniGeneiHs.296169.

Genome annotation databases

EnsembliENST00000366690; ENSP00000355651; ENSG00000168118.
GeneIDi5867.
KEGGihsa:5867.
UCSCiuc001hth.5. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28211 mRNA. Translation: AAA60244.1. Frameshift.
AY585832 mRNA. Translation: AAT91347.1.
AF498934 mRNA. Translation: AAM21082.1.
AK313807 mRNA. Translation: BAG36543.1.
AL162595, AL117350 Genomic DNA. Translation: CAI19037.1.
AL117350, AL162595 Genomic DNA. Translation: CAI22870.1.
CH471098 Genomic DNA. Translation: EAW69890.1.
BC002438 mRNA. Translation: AAH02438.1.
BC004309 mRNA. Translation: AAH04309.1.
CCDSiCCDS31050.1.
PIRiE34323.
RefSeqiNP_001258927.1. NM_001271998.1.
NP_004569.2. NM_004578.3.
UniGeneiHs.296169.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YU9X-ray2.07A7-177[»]
1Z0KX-ray1.92A/C7-177[»]
2BMDX-ray1.80A6-189[»]
2BMEX-ray1.57A/B/C/D6-189[»]
ProteinModelPortaliP20338.
SMRiP20338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111805. 38 interactors.
DIPiDIP-372N.
IntActiP20338. 21 interactors.
MINTiMINT-1376149.
STRINGi9606.ENSP00000355651.

PTM databases

iPTMnetiP20338.
PhosphoSitePlusiP20338.

Polymorphism and mutation databases

DMDMi460018296.

Proteomic databases

EPDiP20338.
MaxQBiP20338.
PaxDbiP20338.
PeptideAtlasiP20338.
PRIDEiP20338.

Protocols and materials databases

DNASUi5867.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000366690; ENSP00000355651; ENSG00000168118.
GeneIDi5867.
KEGGihsa:5867.
UCSCiuc001hth.5. human.

Organism-specific databases

CTDi5867.
DisGeNETi5867.
GeneCardsiRAB4A.
HGNCiHGNC:9781. RAB4A.
HPAiCAB004990.
MIMi179511. gene.
neXtProtiNX_P20338.
OpenTargetsiENSG00000168118.
PharmGKBiPA34141.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0086. Eukaryota.
ENOG410XQ9X. LUCA.
GeneTreeiENSGT00760000118841.
HOVERGENiHBG009351.
InParanoidiP20338.
KOiK07879.
OMAiMVKLQIW.
OrthoDBiEOG091G0QOB.
PhylomeDBiP20338.
TreeFamiTF300032.

Enzyme and pathway databases

ReactomeiR-HSA-1445148. Translocation of GLUT4 to the plasma membrane.
R-HSA-1660499. Synthesis of PIPs at the plasma membrane.
R-HSA-8875656. MET receptor recycling.
SignaLinkiP20338.
SIGNORiP20338.

Miscellaneous databases

ChiTaRSiRAB4A. human.
EvolutionaryTraceiP20338.
GeneWikiiRAB4A.
GenomeRNAii5867.
PROiP20338.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168118.
CleanExiHS_RAB4A.
ExpressionAtlasiP20338. baseline and differential.
GenevisibleiP20338. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAB4A_HUMAN
AccessioniPrimary (citable) accession number: P20338
Secondary accession number(s): Q5T7P7, Q9BQ44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: March 6, 2013
Last modified: November 30, 2016
This is version 183 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-6 is the initiator.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.