P20337 (RAB3B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-3B

Gene names

Name:

RAB3B

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protein transport. Probably involved in vesicular traffic By similarity.
Subunit structure	Binds RIMS1 and RIMS2 By similarity.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 219

219

Ras-related protein Rab-3B

PRO_0000121081

Regions

Nucleotide binding

29 – 37

9

GTP

Nucleotide binding

77 – 81

5

GTP By similarity

Nucleotide binding

135 – 138

4

GTP

Nucleotide binding

165 – 167

3

GTP

Motif

51 – 59

9

Effector region By similarity

Amino acid modifications

Modified residue

188

1

Phosphoserine By similarity

Modified residue

219

1

Cysteine methyl ester By similarity

Lipidation

217

1

S-geranylgeranyl cysteine By similarity

Lipidation

219

1

S-geranylgeranyl cysteine By similarity

Experimental info

Sequence conflict

9

1

T → H in AAA60243. Ref.1

Sequence conflict

40

1

F → L in AAA60243. Ref.1

Secondary structure

1

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219

Helix Strand Turn

Details...

Beta strand

19 – 21

3

Beta strand

23 – 28

6

Helix

36 – 45

10

Beta strand

51 – 55

5

Beta strand

59 – 66

8

Turn

67 – 70

4

Beta strand

71 – 76

6

Helix

85 – 92

8

Beta strand

99 – 101

3

Helix

107 – 111

5

Helix

113 – 123

11

Beta strand

131 – 133

3

Helix

140 – 142

3

Helix

152 – 156

5

Turn

166 – 169

4

Helix

172 – 186

15

Sequences

	Sequence		Length	Mass (Da)
	P20337 [UniParc]. Last modified October 31, 2003. Version 2. Checksum: B73A014B4295A60D	FASTA	219	24,758
	10 20 30 40 50 60 MASVTDGKTG VKDASDQNFD YMFKLLIIGN SSVGKTSFLF RYADDTFTPA FVSTVGIDFK 70 80 90 100 110 120 VKTVYRHEKR VKLQIWDTAG QERYRTITTA YYRGAMGFIL MYDITNEESF NAVQDWATQI 130 140 150 160 170 180 KTYSWDNAQV ILVGNKCDME EERVVPTEKG QLLAEQLGFD FFEASAKENI SVRQAFERLV 190 200 210 DAICDKMSDS LDTDPSMLGS SKNTRLSDTP PLLQQNCSC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion." Zahraoui A., Touchot N., Chardin P., Tavitian A. J. Biol. Chem. 264:12394-12401(1989) [PubMed: 2501306] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[5]	"Crystal structure of human RAB3B GTPase bound with GDP." Structural genomics consortium (SGC) Submitted (FEB-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 18-190 IN COMPLEX WITH GDP.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M28214 mRNA. Translation: AAA60243.1.
AF498932 mRNA. Translation: AAM21080.1.
AL589663, AL445685 Genomic DNA. Translation: CAH74098.1.
AL445685, AL589663 Genomic DNA. Translation: CAI17027.1.
BC005035 mRNA. Translation: AAH05035.1.

IPI

IPI00300562.

PIR

D34323.

RefSeq

NP_002858.2. NM_002867.3.

UniGene

Hs.123072.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3DZ8	X-ray	1.90	A	18-190	[»]

ProteinModelPortal

P20337.

SMR

P20337. Positions 18-187.

ModBase

Search...

Protein-protein interaction databases

STRING

P20337.

PTM databases

PhosphoSite

P20337.

Polymorphism databases

DMDM

38258903.

Proteomic databases

PeptideAtlas

P20337.

PRIDE

P20337.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000371655; ENSP00000360718; ENSG00000169213.

GeneID

5865.

KEGG

hsa:5865.

UCSC

uc001cth.1. human.

Organism-specific databases

CTD

5865.

GeneCards

GC01M052373.

H-InvDB

HIX0199885.

HGNC

HGNC:9778. RAB3B.

HPA

CAB023293.
HPA003159.

MIM

179510. gene.

neXtProt

NX_P20337.

PharmGKB

PA34133.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG19658.

GeneTree

ENSGT00600000084147.

HOGENOM

HBG745225.

HOVERGEN

HBG009351.

InParanoid

P20337.

OMA

GKDASDQ.

OrthoDB

EOG4TB4C6.

PhylomeDB

P20337.

Gene expression databases

ArrayExpress

P20337.

Bgee

P20337.

CleanEx

HS_RAB3B.

Genevestigator

P20337.

GermOnline

ENSG00000169213. Homo sapiens.

Family and domain databases

InterPro

IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]

KO

K06108.

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.

SMART

SM00175. RAB. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. Small_GTP. 1 hit.

PROSITE

PS51419. RAB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

22778.

SOURCE

Search...

Entry information

Entry name

RAB3B_HUMAN

Accession

Primary (citable) accession number: P20337
Secondary accession number(s): Q5VUL2, Q9BSI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	October 31, 2003
Last modified:	January 25, 2012
This is version 120 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.