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P20337 (RAB3B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 141. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras-related protein Rab-3B
Gene names
Name:RAB3B
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length219 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protein transport. Probably involved in vesicular traffic By similarity.

Subunit structure

Interacts with RIMS1, RIMS2, RPH3A and RPH3AL By similarity.

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side Potential.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCell membrane
Membrane
   LigandGTP-binding
Nucleotide-binding
   PTMLipoprotein
Methylation
Phosphoprotein
Prenylation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processantigen processing and presentation

Inferred from mutant phenotype PubMed 19717423. Source: UniProt

peptidyl-cysteine methylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of dopamine uptake involved in synaptic transmission

Inferred from direct assay PubMed 20007772. Source: UniProt

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of exocytosis

Inferred from electronic annotation. Source: Ensembl

regulation of vesicle size

Inferred from direct assay PubMed 20007772. Source: UniProt

small GTPase mediated signal transduction

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 20807725. Source: UniProt

cytosol

Inferred from electronic annotation. Source: Ensembl

extracellular vesicular exosome

Inferred from direct assay PubMed 19056867. Source: UniProt

perinuclear region of cytoplasm

Inferred from direct assay PubMed 19717423. Source: UniProt

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

secretory granule

Inferred from electronic annotation. Source: Ensembl

synaptic vesicle

Inferred from electronic annotation. Source: Ensembl

vesicle

Inferred from direct assay PubMed 19717423. Source: UniProt

   Molecular_functionGDP binding

Inferred from direct assay PubMed 22321395. Source: UniProt

GTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 219219Ras-related protein Rab-3B
PRO_0000121081

Regions

Nucleotide binding29 – 379GTP
Nucleotide binding48 – 547GTP By similarity
Nucleotide binding77 – 815GTP By similarity
Nucleotide binding135 – 1384GTP
Nucleotide binding165 – 1673GTP
Motif51 – 599Effector region By similarity

Amino acid modifications

Modified residue1901Phosphoserine Ref.5
Modified residue2191Cysteine methyl ester By similarity
Lipidation2171S-geranylgeranyl cysteine By similarity
Lipidation2191S-geranylgeranyl cysteine By similarity

Experimental info

Sequence conflict91T → H in AAA60243. Ref.1
Sequence conflict401F → L in AAA60243. Ref.1

Secondary structure

................................... 219
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P20337 [UniParc].

Last modified October 31, 2003. Version 2.
Checksum: B73A014B4295A60D

FASTA21924,758
        10         20         30         40         50         60 
MASVTDGKTG VKDASDQNFD YMFKLLIIGN SSVGKTSFLF RYADDTFTPA FVSTVGIDFK 

        70         80         90        100        110        120 
VKTVYRHEKR VKLQIWDTAG QERYRTITTA YYRGAMGFIL MYDITNEESF NAVQDWATQI 

       130        140        150        160        170        180 
KTYSWDNAQV ILVGNKCDME EERVVPTEKG QLLAEQLGFD FFEASAKENI SVRQAFERLV 

       190        200        210 
DAICDKMSDS LDTDPSMLGS SKNTRLSDTP PLLQQNCSC 

« Hide

References

« Hide 'large scale' references
[1]"The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion."
Zahraoui A., Touchot N., Chardin P., Tavitian A.
J. Biol. Chem. 264:12394-12401(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[5]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-190, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"Crystal structure of human RAB3B GTPase bound with GDP."
Structural genomics consortium (SGC)
Submitted (FEB-2009) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 18-190 IN COMPLEX WITH GDP.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M28214 mRNA. Translation: AAA60243.1.
AF498932 mRNA. Translation: AAM21080.1.
AL589663, AL445685 Genomic DNA. Translation: CAH74098.1.
AL445685, AL589663 Genomic DNA. Translation: CAI17027.1.
BC005035 mRNA. Translation: AAH05035.1.
PIRD34323.
RefSeqNP_002858.2. NM_002867.3.
UniGeneHs.123072.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3DZ8X-ray1.90A18-190[»]
ProteinModelPortalP20337.
SMRP20337. Positions 18-187.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111803. 7 interactions.
STRING9606.ENSP00000360718.

PTM databases

PhosphoSiteP20337.

Polymorphism databases

DMDM38258903.

Proteomic databases

PaxDbP20337.
PeptideAtlasP20337.
PRIDEP20337.

Protocols and materials databases

DNASU5865.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000371655; ENSP00000360718; ENSG00000169213.
GeneID5865.
KEGGhsa:5865.
UCSCuc001cth.3. human.

Organism-specific databases

CTD5865.
GeneCardsGC01M052373.
HGNCHGNC:9778. RAB3B.
HPACAB023293.
HPA003159.
MIM179510. gene.
neXtProtNX_P20337.
PharmGKBPA34133.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1100.
HOGENOMHOG000233968.
HOVERGENHBG009351.
InParanoidP20337.
KOK06108.
OMAWFDFFEA.
OrthoDBEOG7JQBPC.
PhylomeDBP20337.
TreeFamTF313199.

Gene expression databases

BgeeP20337.
CleanExHS_RAB3B.
GenevestigatorP20337.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP20337.
GeneWikiRAB3B.
GenomeRNAi5865.
NextBio22778.
PROP20337.
SOURCESearch...

Entry information

Entry nameRAB3B_HUMAN
AccessionPrimary (citable) accession number: P20337
Secondary accession number(s): Q5VUL2, Q9BSI1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: October 31, 2003
Last modified: April 16, 2014
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM