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P20336

- RAB3A_HUMAN

UniProt

P20336 - RAB3A_HUMAN

Protein

Ras-related protein Rab-3A

Gene

RAB3A

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 162 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi29 – 368GTPBy similarity
    Nucleotide bindingi48 – 547GTPBy similarity
    Nucleotide bindingi77 – 815GTPBy similarity
    Nucleotide bindingi135 – 1384GTPBy similarity

    GO - Molecular functioni

    1. ATPase activator activity Source: Ensembl
    2. GTPase activity Source: UniProtKB
    3. GTP binding Source: UniProtKB-KW
    4. protein binding Source: UniProtKB

    GO - Biological processi

    1. axonogenesis Source: Ensembl
    2. glutamate secretion Source: Reactome
    3. GTP catabolic process Source: GOC
    4. lung development Source: Ensembl
    5. maintenance of presynaptic active zone structure Source: Ensembl
    6. mitochondrion organization Source: Ensembl
    7. neuromuscular synaptic transmission Source: Ensembl
    8. neurotransmitter secretion Source: Reactome
    9. post-embryonic development Source: Ensembl
    10. protein transport Source: UniProtKB-KW
    11. regulation of synaptic vesicle fusion to presynaptic membrane Source: Ensembl
    12. respiratory system process Source: Ensembl
    13. response to electrical stimulus Source: Ensembl
    14. sensory perception of touch Source: Ensembl
    15. small GTPase mediated signal transduction Source: InterPro
    16. synaptic transmission Source: Reactome
    17. synaptic vesicle exocytosis Source: Ensembl
    18. synaptic vesicle maturation Source: Ensembl

    Keywords - Biological processi

    Exocytosis, Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_12591. Glutamate Neurotransmitter Release Cycle.
    REACT_15293. Dopamine Neurotransmitter Release Cycle.
    REACT_15309. Acetylcholine Neurotransmitter Release Cycle.
    REACT_15418. Norepinephrine Neurotransmitter Release Cycle.
    REACT_15425. Serotonin Neurotransmitter Release Cycle.
    REACT_23947. GABA synthesis, release, reuptake and degradation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related protein Rab-3A
    Gene namesi
    Name:RAB3A
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:9777. RAB3A.

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

    GO - Cellular componenti

    1. acrosomal vesicle Source: Ensembl
    2. clathrin-sculpted acetylcholine transport vesicle membrane Source: Reactome
    3. clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane Source: Reactome
    4. clathrin-sculpted glutamate transport vesicle membrane Source: Reactome
    5. clathrin-sculpted monoamine transport vesicle membrane Source: Reactome
    6. cytosol Source: Ensembl
    7. plasma membrane Source: Reactome
    8. protein complex Source: Ensembl
    9. synaptic vesicle Source: ProtInc

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA34132.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 220220Ras-related protein Rab-3APRO_0000121076Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi218 – 2181S-geranylgeranyl cysteine2 Publications
    Modified residuei220 – 2201Cysteine methyl ester1 Publication
    Lipidationi220 – 2201S-geranylgeranyl cysteine2 Publications

    Keywords - PTMi

    Lipoprotein, Methylation, Prenylation

    Proteomic databases

    MaxQBiP20336.
    PaxDbiP20336.
    PRIDEiP20336.

    PTM databases

    PhosphoSiteiP20336.

    Expressioni

    Tissue specificityi

    Specifically expressed in brain.

    Gene expression databases

    ArrayExpressiP20336.
    BgeeiP20336.
    CleanExiHS_RAB3A.
    GenevestigatoriP20336.

    Organism-specific databases

    HPAiCAB009949.
    HPA003160.

    Interactioni

    Subunit structurei

    Heterodimer with RIMS2. Part of a ternary complex involving PCLO and EPAC2. Interacts with RPH3A and RPH3AL. Interacts with the exocyst complex through SEC15. Binds SYTL4 and RIMS1. Interacts with RAB3IP. Interacts with SGSM1 and SGSM3 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi111802. 23 interactions.
    IntActiP20336. 4 interactions.
    MINTiMINT-3009239.
    STRINGi9606.ENSP00000222256.

    Structurei

    3D structure databases

    ProteinModelPortaliP20336.
    SMRiP20336. Positions 18-186.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi51 – 599Effector regionBy similarity

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000233968.
    HOVERGENiHBG009351.
    InParanoidiP20336.
    KOiK07882.
    OMAiQLTEQPA.
    OrthoDBiEOG7JQBPC.
    PhylomeDBiP20336.
    TreeFamiTF313199.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P20336-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASATDSRYG QKESSDQNFD YMFKILIIGN SSVGKTSFLF RYADDSFTPA    50
    FVSTVGIDFK VKTIYRNDKR IKLQIWDTAG QERYRTITTA YYRGAMGFIL 100
    MYDITNEESF NAVQDWSTQI KTYSWDNAQV LLVGNKCDME DERVVSSERG 150
    RQLADHLGFE FFEASAKDNI NVKQTFERLV DVICEKMSES LDTADPAVTG 200
    AKQGPQLSDQ QVPPHQDCAC 220
    Length:220
    Mass (Da):24,984
    Last modified:February 1, 1991 - v1
    Checksum:i08B59F8C9BD2EB40
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti70 – 701R → K in AAF67748. (PubMed:10574328)Curated
    Sequence conflicti180 – 1801V → E in AAF67748. (PubMed:10574328)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M28210 mRNA. Translation: AAA60242.1.
    AF157809
    , AF157806, AF157807, AF157808 Genomic DNA. Translation: AAD46811.1.
    AF254795 mRNA. Translation: AAF67748.1.
    AF498931 mRNA. Translation: AAM21079.1.
    AK289559 mRNA. Translation: BAF82248.1.
    AC068499 Genomic DNA. Translation: AAF67385.1.
    CH471106 Genomic DNA. Translation: EAW84672.1.
    BC011782 mRNA. Translation: AAH11782.1.
    CCDSiCCDS12372.1.
    PIRiC34323.
    RefSeqiNP_002857.1. NM_002866.4.
    UniGeneiHs.27744.

    Genome annotation databases

    EnsembliENST00000222256; ENSP00000222256; ENSG00000105649.
    GeneIDi5864.
    KEGGihsa:5864.
    UCSCiuc002nie.2. human.

    Polymorphism databases

    DMDMi131801.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M28210 mRNA. Translation: AAA60242.1 .
    AF157809
    , AF157806 , AF157807 , AF157808 Genomic DNA. Translation: AAD46811.1 .
    AF254795 mRNA. Translation: AAF67748.1 .
    AF498931 mRNA. Translation: AAM21079.1 .
    AK289559 mRNA. Translation: BAF82248.1 .
    AC068499 Genomic DNA. Translation: AAF67385.1 .
    CH471106 Genomic DNA. Translation: EAW84672.1 .
    BC011782 mRNA. Translation: AAH11782.1 .
    CCDSi CCDS12372.1.
    PIRi C34323.
    RefSeqi NP_002857.1. NM_002866.4.
    UniGenei Hs.27744.

    3D structure databases

    ProteinModelPortali P20336.
    SMRi P20336. Positions 18-186.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111802. 23 interactions.
    IntActi P20336. 4 interactions.
    MINTi MINT-3009239.
    STRINGi 9606.ENSP00000222256.

    PTM databases

    PhosphoSitei P20336.

    Polymorphism databases

    DMDMi 131801.

    Proteomic databases

    MaxQBi P20336.
    PaxDbi P20336.
    PRIDEi P20336.

    Protocols and materials databases

    DNASUi 5864.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000222256 ; ENSP00000222256 ; ENSG00000105649 .
    GeneIDi 5864.
    KEGGi hsa:5864.
    UCSCi uc002nie.2. human.

    Organism-specific databases

    CTDi 5864.
    GeneCardsi GC19M018307.
    HGNCi HGNC:9777. RAB3A.
    HPAi CAB009949.
    HPA003160.
    MIMi 179490. gene.
    neXtProti NX_P20336.
    PharmGKBi PA34132.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000233968.
    HOVERGENi HBG009351.
    InParanoidi P20336.
    KOi K07882.
    OMAi QLTEQPA.
    OrthoDBi EOG7JQBPC.
    PhylomeDBi P20336.
    TreeFami TF313199.

    Enzyme and pathway databases

    Reactomei REACT_12591. Glutamate Neurotransmitter Release Cycle.
    REACT_15293. Dopamine Neurotransmitter Release Cycle.
    REACT_15309. Acetylcholine Neurotransmitter Release Cycle.
    REACT_15418. Norepinephrine Neurotransmitter Release Cycle.
    REACT_15425. Serotonin Neurotransmitter Release Cycle.
    REACT_23947. GABA synthesis, release, reuptake and degradation.

    Miscellaneous databases

    GeneWikii RAB3A.
    GenomeRNAii 5864.
    NextBioi 22774.
    PROi P20336.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P20336.
    Bgeei P20336.
    CleanExi HS_RAB3A.
    Genevestigatori P20336.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion."
      Zahraoui A., Touchot N., Chardin P., Tavitian A.
      J. Biol. Chem. 264:12394-12401(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Genomic organisation of the human cyclic AMP-specific phosphodiesterase PDE4C gene and its chromosomal localisation to 19p13.1, between RAB3A and JUND."
      Sullivan M., Olsen A.S., Houslay M.D.
      Cell. Signal. 11:735-742(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Functional cloning and characterization of human fetal brain cDNAs."
      Liu Y., Li J., He J.J.
      Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Fetal brain.
    4. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Puhl H.L. III, Ikeda S.R., Aronstam R.S.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Cerebellum.
    6. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.
    9. Cited for: ISOPRENYLATION AT CYS-218 AND CYS-220, METHYLATION AT CYS-220.
    10. "Rab geranylgeranyl transferase catalyzes the geranylgeranylation of adjacent cysteines in the small GTPases Rab1A, Rab3A, and Rab5A."
      Farnsworth C.C., Seabra M.C., Ericsson L.H., Gelb M.H., Glomset J.A.
      Proc. Natl. Acad. Sci. U.S.A. 91:11963-11967(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: ISOPRENYLATION AT CYS-218 AND CYS-220, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiRAB3A_HUMAN
    AccessioniPrimary (citable) accession number: P20336
    Secondary accession number(s): A8K0J4, Q9NYE1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 162 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3