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Protein

Endolysin

Gene

3.5

Organism
Enterobacteria phage T3 (Bacteriophage T3)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Plays an important role in the switch between viral transcription and genome replication. Once produced in sufficient amount, interacts with and inhibits the viral RNA polymerase that becomes unable to produce additional late transcripts. This lysozyme-polymerase complex in turn plays an active role in viral genome replication and packaging.UniRule annotation
Endolysin with amidase activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and breaking down the peptidoglycan layer.UniRule annotation

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Zn2+ is required for amidase activity.UniRule annotation

Enzyme regulationi

Binding to the viral RNA polymerase inhibits amidase activity.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi18ZincUniRule annotation1
Sitei47Essential for amidase activity and zinc hydrate coordinationUniRule annotation1
Metal bindingi123ZincUniRule annotation1
Metal bindingi131ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntimicrobial, Bacteriolytic enzyme, Hydrolase
Biological processCytolysis, Host cell lysis by virus, Virus exit from host cell
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
EndolysinUniRule annotation (EC:3.5.1.28UniRule annotation)
Alternative name(s):
N-acetylmuramoyl-L-alanine amidaseUniRule annotation
T3 lysozyme
Gene namesi
Name:3.5
OrganismiEnterobacteria phage T3 (Bacteriophage T3)
Taxonomic identifieri10759 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7virus
Virus hostiEscherichia coli [TaxID: 562]

Subcellular locationi

  • Host cytoplasm UniRule annotation

  • Note: The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by hostBy similarity
ChainiPRO_00001644092 – 151EndolysinAdd BLAST150

Expressioni

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Interacts with the viral RNA polymerase.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP20331.
SMRiP20331.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.UniRule annotation

Family and domain databases

CDDicd06583. PGRP. 1 hit.
Gene3Di3.40.80.10. 1 hit.
HAMAPiMF_04111. ENDOLYSIN_T7. 1 hit.
InterProiView protein in InterPro
IPR002502. Amidase_domain.
IPR034689. Endolysin_T7_type.
IPR015510. PGRP.
IPR006619. PGRP_domain_met/bac.
PANTHERiPTHR11022. PTHR11022. 1 hit.
PfamiView protein in Pfam
PF01510. Amidase_2. 1 hit.
SMARTiView protein in SMART
SM00644. Ami_2. 1 hit.
SM00701. PGRP. 1 hit.
SUPFAMiSSF55846. SSF55846. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20331-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKVQFKPRA TTEAIFVHCS ATKPSQNVGV REIRQWHKEQ GWLDVGYHFI
60 70 80 90 100
IKRDGTVEAG RDELAVGSHA KGYNHNSIGV CLVGGIDDKG KFDANFTPAQ
110 120 130 140 150
MQSLRSLLVT LLAKYEGSVL RAHHDVAPKA CPSFDLKRWW EKNELVTSDR

G
Length:151
Mass (Da):16,874
Last modified:January 23, 2007 - v3
Checksum:i2C0880FFC920DE9E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17255 Genomic DNA. Translation: CAA35133.1.
PIRiS07506.
RefSeqiNP_523313.1. NC_003298.1.

Genome annotation databases

GeneIDi927411.
KEGGivg:927411.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiENLYS_BPT3
AccessioniPrimary (citable) accession number: P20331
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: July 5, 2017
This is version 86 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families