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P20331

- NAAA_BPT3

UniProt

P20331 - NAAA_BPT3

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Protein
N-acetylmuramoyl-L-alanine amidase
Gene
3.5
Organism
Enterobacteria phage T3 (Bacteriophage T3)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This protein is not required for lysis but plays an important role in DNA synthesis. It is known to detach the host chromosome from the bacterial membrane to which it is normally bound. It is a bifunctional protein that cuts amide bonds in the bacterial cell wall and binds to and inhibits transcription by T3 RNA polymerase By similarity.

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Cofactori

Zinc; required for amidase activity By similarity.

Enzyme regulationi

Binding of T3 RNA polymerase inhibits amidase activity By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi18 – 181Zinc By similarity
Sitei47 – 471Essential for amidase activity and zinc hydrate coordination By similarity
Metal bindingi123 – 1231Zinc By similarity
Metal bindingi131 – 1311Zinc By similarity

GO - Molecular functioni

  1. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. peptidoglycan catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylmuramoyl-L-alanine amidase (EC:3.5.1.28)
Alternative name(s):
T3 lysozyme
Gene namesi
Name:3.5
OrganismiEnterobacteria phage T3 (Bacteriophage T3)
Taxonomic identifieri10759 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7likevirus
Virus hostiEscherichia coli [TaxID: 562]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; by host By similarity
Chaini2 – 151150N-acetylmuramoyl-L-alanine amidase
PRO_0000164409Add
BLAST

Expressioni

Keywords - Developmental stagei

Late protein

Structurei

3D structure databases

ProteinModelPortaliP20331.
SMRiP20331. Positions 3-151.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
IPR015510. PGRP.
[Graphical view]
PANTHERiPTHR11022. PTHR11022. 1 hit.
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
SMARTiSM00644. Ami_2. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20331-1 [UniParc]FASTAAdd to Basket

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MAKVQFKPRA TTEAIFVHCS ATKPSQNVGV REIRQWHKEQ GWLDVGYHFI    50
IKRDGTVEAG RDELAVGSHA KGYNHNSIGV CLVGGIDDKG KFDANFTPAQ 100
MQSLRSLLVT LLAKYEGSVL RAHHDVAPKA CPSFDLKRWW EKNELVTSDR 150
G 151
Length:151
Mass (Da):16,874
Last modified:January 23, 2007 - v3
Checksum:i2C0880FFC920DE9E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17255 Genomic DNA. Translation: CAA35133.1.
PIRiS07506.
RefSeqiNP_523313.1. NC_003298.1.

Genome annotation databases

GeneIDi927411.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17255 Genomic DNA. Translation: CAA35133.1 .
PIRi S07506.
RefSeqi NP_523313.1. NC_003298.1.

3D structure databases

ProteinModelPortali P20331.
SMRi P20331. Positions 3-151.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 927411.

Family and domain databases

Gene3Di 3.40.80.10. 1 hit.
InterProi IPR002502. Amidase_domain.
IPR015510. PGRP.
[Graphical view ]
PANTHERi PTHR11022. PTHR11022. 1 hit.
Pfami PF01510. Amidase_2. 1 hit.
[Graphical view ]
SMARTi SM00644. Ami_2. 1 hit.
[Graphical view ]
SUPFAMi SSF55846. SSF55846. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Sequence of bacteriophage T3 DNA from gene 2.5 through gene 9."
    Beck P.J., Gonzalez S., Ward C.L., Molineux I.J.
    J. Mol. Biol. 210:687-701(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Luria.

Entry informationi

Entry nameiNAAA_BPT3
AccessioniPrimary (citable) accession number: P20331
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 70 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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