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P20331

- NAAA_BPT3

UniProt

P20331 - NAAA_BPT3

Protein

N-acetylmuramoyl-L-alanine amidase

Gene

3.5

Organism
Enterobacteria phage T3 (Bacteriophage T3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
  1. Functioni

    This protein is not required for lysis but plays an important role in DNA synthesis. It is known to detach the host chromosome from the bacterial membrane to which it is normally bound. It is a bifunctional protein that cuts amide bonds in the bacterial cell wall and binds to and inhibits transcription by T3 RNA polymerase By similarity.By similarity

    Catalytic activityi

    Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

    Cofactori

    Zinc; required for amidase activity.By similarity

    Enzyme regulationi

    Binding of T3 RNA polymerase inhibits amidase activity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi18 – 181ZincBy similarity
    Sitei47 – 471Essential for amidase activity and zinc hydrate coordinationBy similarity
    Metal bindingi123 – 1231ZincBy similarity
    Metal bindingi131 – 1311ZincBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. peptidoglycan catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acetylmuramoyl-L-alanine amidase (EC:3.5.1.28)
    Alternative name(s):
    T3 lysozyme
    Gene namesi
    Name:3.5
    OrganismiEnterobacteria phage T3 (Bacteriophage T3)
    Taxonomic identifieri10759 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7likevirus
    Virus hostiEscherichia coli [TaxID: 562]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed; by hostBy similarity
    Chaini2 – 151150N-acetylmuramoyl-L-alanine amidasePRO_0000164409Add
    BLAST

    Expressioni

    Keywords - Developmental stagei

    Late protein

    Structurei

    3D structure databases

    ProteinModelPortaliP20331.
    SMRiP20331. Positions 3-151.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.80.10. 1 hit.
    InterProiIPR002502. Amidase_domain.
    IPR015510. PGRP.
    [Graphical view]
    PANTHERiPTHR11022. PTHR11022. 1 hit.
    PfamiPF01510. Amidase_2. 1 hit.
    [Graphical view]
    SMARTiSM00644. Ami_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF55846. SSF55846. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P20331-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKVQFKPRA TTEAIFVHCS ATKPSQNVGV REIRQWHKEQ GWLDVGYHFI    50
    IKRDGTVEAG RDELAVGSHA KGYNHNSIGV CLVGGIDDKG KFDANFTPAQ 100
    MQSLRSLLVT LLAKYEGSVL RAHHDVAPKA CPSFDLKRWW EKNELVTSDR 150
    G 151
    Length:151
    Mass (Da):16,874
    Last modified:January 23, 2007 - v3
    Checksum:i2C0880FFC920DE9E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17255 Genomic DNA. Translation: CAA35133.1.
    PIRiS07506.
    RefSeqiNP_523313.1. NC_003298.1.

    Genome annotation databases

    GeneIDi927411.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17255 Genomic DNA. Translation: CAA35133.1 .
    PIRi S07506.
    RefSeqi NP_523313.1. NC_003298.1.

    3D structure databases

    ProteinModelPortali P20331.
    SMRi P20331. Positions 3-151.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 927411.

    Family and domain databases

    Gene3Di 3.40.80.10. 1 hit.
    InterProi IPR002502. Amidase_domain.
    IPR015510. PGRP.
    [Graphical view ]
    PANTHERi PTHR11022. PTHR11022. 1 hit.
    Pfami PF01510. Amidase_2. 1 hit.
    [Graphical view ]
    SMARTi SM00644. Ami_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55846. SSF55846. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of bacteriophage T3 DNA from gene 2.5 through gene 9."
      Beck P.J., Gonzalez S., Ward C.L., Molineux I.J.
      J. Mol. Biol. 210:687-701(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Luria.

    Entry informationi

    Entry nameiNAAA_BPT3
    AccessioniPrimary (citable) accession number: P20331
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 71 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3