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Protein

DNA primase/helicase

Gene

4

Organism
Enterobacteria phage T3 (Bacteriophage T3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA primase synthesizes RNA primers necessary for replication by DNA polymerase. It also functions as an ATP-dependent unwinding protein. Has a 5'->3' helicase activity; 4a also has a DNA primase activity (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri15 – 37C4-type; may recognize priming sitesSequence analysisAdd BLAST23
Nucleotide bindingi311 – 318ATPPROSITE-ProRule annotation8

GO - Molecular functioni

Keywordsi

Molecular functionHelicase, Hydrolase, Nucleotidyltransferase, Transferase
Biological processDNA replication, Transcription
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA primase/helicase (EC:2.7.7.-, EC:3.6.4.12)
Gene namesi
Name:4
OrganismiEnterobacteria phage T3 (Bacteriophage T3)
Taxonomic identifieri10759 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7virus
Virus hostiEscherichia coli [TaxID: 562]

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase, Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000033531 – 566DNA primase/helicaseAdd BLAST566

Expressioni

Keywords - Developmental stagei

Late protein

Structurei

3D structure databases

ProteinModelPortaliP20315.
SMRiP20315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini150 – 237ToprimPROSITE-ProRule annotationAdd BLAST88
Domaini280 – 547SF4 helicasePROSITE-ProRule annotationAdd BLAST268

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri15 – 37C4-type; may recognize priming sitesSequence analysisAdd BLAST23

Keywords - Domaini

Zinc-finger

Phylogenomic databases

KOiK17680.

Family and domain databases

CDDicd01029. TOPRIM_primases. 1 hit.
InterProiView protein in InterPro
IPR007694. DNA_helicase_DnaB-like_C.
IPR027417. P-loop_NTPase.
IPR013237. Phage_T7_Gp4_N.
IPR034154. TOPRIM_DnaG/twinkle.
IPR006171. TOPRIM_domain.
PfamiView protein in Pfam
PF03796. DnaB_C. 1 hit.
SMARTiView protein in SMART
SM00778. Prim_Zn_Ribbon. 1 hit.
SM00493. TOPRIM. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiView protein in PROSITE
PS51199. SF4_HELICASE. 1 hit.
PS50880. TOPRIM. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Chain a (identifier: P20315-1) [UniParc]FASTAAdd to basket
Also known as: 4a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEREDDSIFL FHAPCENCGS SDGNSVYSDG HEWCFVCEHR VPANEEREAK
60 70 80 90 100
LSTRRRTGGS KPMSYDVWNF GDSNGRYSDL TARGISKETC QKAGYWLAKV
110 120 130 140 150
DNRMYQVADY RDQNGSIVSQ KVRDKDKNFK TTGSHKSDAL FLKHLWSGGK
160 170 180 190 200
KIVVTEGEID ALTVMELQDC KYPVVSLGHG ASAAKKTCAA NYEYFDQFEQ
210 220 230 240 250
IILMFDMDDA GRKAVEEAAQ VLPAGKVRVA VLPCKDANEC HIMGEDKAIL
260 270 280 290 300
EQVWNANPWV PDGVVSALSL KDRVKEAMTS EDAVGLLFDG CQGLNDRTLG
310 320 330 340 350
ARGGEVVMVT SGSGMGKSTF VRQQALAWGK RMGKRVGLAM LEESVEDTIQ
360 370 380 390 400
DMMGLNNKVR LRQSDEVKKA IAEDGRFDEW YDELFGDDTF HLYDSFAEAE
410 420 430 440 450
ADRLLAKLAY MRTGLGCDVI VLDHISIVVS ASEESDERKM IDRLMTKLKG
460 470 480 490 500
FAKSTGVVLV VICHLKNPEK GKAHEEGRAV SITDLRGSGA LRQLSDTIIA
510 520 530 540 550
LERNQQGDMP NLVLVRLLKC RFTGDTGIAG YMEYNRETGW LEPSSYTGGE
560
GEGDTGWTEQ DGQSDF
Length:566
Mass (Da):62,741
Last modified:February 1, 1991 - v1
Checksum:i7553913C43DDFEBE
GO
Isoform Chain b (identifier: P20315-2) [UniParc]FASTAAdd to basket
Also known as: 4b

The sequence of this isoform differs from the canonical sequence as follows:
     1-62: Missing.

Note: No experimental confirmation available.
Show »
Length:504
Mass (Da):55,727
Checksum:iCAF77BC2CB0BD98D
GO

Sequence cautioni

The sequence CAA35136 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0186821 – 62Missing in isoform Chain b. CuratedAdd BLAST62

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17255 Genomic DNA. Translation: CAA35135.1.
X17255 Genomic DNA. Translation: CAA35136.1. Different initiation.
PIRiS07508.
RefSeqiNP_523315.1. NC_003298.1. [P20315-1]
NP_523316.1. NC_003298.1. [P20315-2]

Genome annotation databases

GeneIDi927429.
927458.
KEGGivg:927429.
vg:927458.

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPRIM_BPT3
AccessioniPrimary (citable) accession number: P20315
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: June 7, 2017
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi