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Protein

DNA-directed DNA polymerase

Gene

5

Organism
Enterobacteria phage T3 (Bacteriophage T3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Replicates viral genomic DNA. This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'-5' direction.UniRule annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi5Magnesium 1; catalytic; for 3'-5' exonuclease activityUniRule annotation1
Metal bindingi5Magnesium 2; catalytic; for 3'-5' exonuclease activityUniRule annotation1
Metal bindingi7Magnesium 2; catalytic; for 3'-5' exonuclease activityUniRule annotation1
Metal bindingi174Magnesium 2; catalytic; for 3'-5' exonuclease activityUniRule annotation1
Metal bindingi475Magnesium 3; catalytic; for polymerase activityUniRule annotation1
Metal bindingi475Magnesium 4; catalytic; for polymerase activityUniRule annotation1
Metal bindingi476Magnesium 4; catalytic; via carbonyl oxygen; for polymerase activityUniRule annotation1
Binding sitei506SubstrateUniRule annotation1
Binding sitei518SubstrateUniRule annotation1
Binding sitei522SubstrateUniRule annotation1
Binding sitei526SubstrateUniRule annotation1
Metal bindingi654Magnesium 3; catalytic; for polymerase activityUniRule annotation1
Metal bindingi654Magnesium 4; catalytic; for polymerase activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase
Biological processDNA replication, Viral DNA replication
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed DNA polymeraseUniRule annotation (EC:2.7.7.7UniRule annotationBy similarity, EC:3.1.11.-UniRule annotationBy similarity)
Alternative name(s):
Gene product 5
Short name:
Gp5
Gene namesi
Name:5
OrganismiEnterobacteria phage T3 (Bacteriophage T3)
Taxonomic identifieri10759 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7virus
Virus hostiEscherichia coli [TaxID: 562]

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001012681 – 704DNA-directed DNA polymeraseAdd BLAST704

Interactioni

Subunit structurei

Composed of two subunits. One is encoded by the phage and the other is encoded by the host thioredoxin.By similarity

Structurei

3D structure databases

ProteinModelPortaliP20311.
SMRiP20311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 1873'-5'exonucleaseUniRule annotationAdd BLAST187
Regioni202 – 704PolymeraseUniRule annotationAdd BLAST503

Sequence similaritiesi

Belongs to the DNA polymerase type-A family.UniRule annotation

Phylogenomic databases

KOiK21238.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_04101. DPOL_T7. 1 hit.
InterProiView protein in InterPro
IPR019760. DNA-dir_DNA_pol_A_CS.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR002298. DNA_polymerase_A.
IPR034699. DPOL_T7.
IPR012337. RNaseH-like_dom.
PfamiView protein in Pfam
PF00476. DNA_pol_A. 1 hit.
PRINTSiPR00868. DNAPOLI.
SMARTiView protein in SMART
SM00482. POLAc. 1 hit.
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiView protein in PROSITE
PS00447. DNA_POLYMERASE_A. 1 hit.

Sequencei

Sequence statusi: Complete.

P20311-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVSDIEANN LLEKVTKFHC GVIYDYRDGE YHSYRPGDFG AYLDALEAEV
60 70 80 90 100
KRGGLIVFHN GHKYDVPALT KLAKLQLNRE FHLPRENCID TLVLSRLIHS
110 120 130 140 150
NLKDTDMGLL RSGKLPGKRF GSHALEAWGY RLGEMKGEYK DDFKRMLEEQ
160 170 180 190 200
GEEYVDGMEW WNFNEEMMDY NVQDVVVTKA LLEKLLSDKH YFPPEIDFTD
210 220 230 240 250
VGYTTFWSES LEAVDVEHRA AWLLAKQERN GFPFDTKAIE ELYVELAARR
260 270 280 290 300
SELLRNLTET FGSWYQPKGG TEMFCHPRTG KPLPKYPRIK IPKVGGIFKK
310 320 330 340 350
PKNKAQREGR EPCELDTREY VAGAPYTPVE HVVFNPSSRD HIQKKLQEAG
360 370 380 390 400
WVPTKFTDKG APVVDDEVLE GVRVDDPEKQ AAIDLIKEYL MIQKRIGQSA
410 420 430 440 450
EGDKAWLRYV AEDGKIHGSV NPNGAVTGRA THAFPNLAQI PGVRSPYGEQ
460 470 480 490 500
CRAAFGAEHH LDGITGKPWV QAGIDASGLE LRCLAHFMAR FDNGEYAHEI
510 520 530 540 550
LNGDIHTKNQ MAAELPTRDN AKTFIYGFLY GAGDEKIGQI VGAGKERGKE
560 570 580 590 600
LKKKFLENTP AIAALRESIQ QTLVESSQWV AGEQQVKWKR RWIKGLDGRK
610 620 630 640 650
VHVRSPHAAL NTLLQSAGAL ICKLWIIKTE EMLVEKGLKH GWDGDFAYMA
660 670 680 690 700
WIHDEIQVAC RTEEIAKTVI EVAQEAMRWV GEHWNFRCLL DTEGKMGANW

KECH
Length:704
Mass (Da):79,985
Last modified:February 1, 1991 - v1
Checksum:iD65DFBD99AE31234
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17255 Genomic DNA. Translation: CAA35140.1.
PIRiS07512.
RefSeqiNP_523320.1. NC_003298.1.

Genome annotation databases

GeneIDi927419.
KEGGivg:927419.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiDPOL_BPT3
AccessioniPrimary (citable) accession number: P20311
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 5, 2017
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families