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Protein

Muscarinic acetylcholine receptor M3

Gene

CHRM3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.1 Publication

GO - Molecular functioni

  • acetylcholine binding Source: UniProtKB
  • drug binding Source: Ensembl
  • G-protein coupled acetylcholine receptor activity Source: UniProtKB
  • phosphatidylinositol phospholipase C activity Source: ProtInc
  • receptor activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

BioCyciZFISH:ENSG00000133019-MONOMER.
ReactomeiR-HSA-390648. Muscarinic acetylcholine receptors.
R-HSA-399997. Acetylcholine regulates insulin secretion.
R-HSA-416476. G alpha (q) signalling events.
SignaLinkiP20309.

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M3
Gene namesi
Name:CHRM3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:1952. CHRM3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 67ExtracellularBy similarityAdd BLAST67
Transmembranei68 – 91Helical; Name=1By similarityAdd BLAST24
Topological domaini92 – 104CytoplasmicBy similarityAdd BLAST13
Transmembranei105 – 130Helical; Name=2By similarityAdd BLAST26
Topological domaini131 – 142ExtracellularBy similarityAdd BLAST12
Transmembranei143 – 164Helical; Name=3By similarityAdd BLAST22
Topological domaini165 – 184CytoplasmicBy similarityAdd BLAST20
Transmembranei185 – 206Helical; Name=4By similarityAdd BLAST22
Topological domaini207 – 229ExtracellularBy similarityAdd BLAST23
Transmembranei230 – 252Helical; Name=5By similarityAdd BLAST23
Topological domaini253 – 491CytoplasmicBy similarityAdd BLAST239
Transmembranei492 – 514Helical; Name=6By similarityAdd BLAST23
Topological domaini515 – 526ExtracellularBy similarityAdd BLAST12
Transmembranei527 – 546Helical; Name=7By similarityAdd BLAST20
Topological domaini547 – 590CytoplasmicBy similarityAdd BLAST44

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Involvement in diseasei

Prune belly syndrome (PBS)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities.
See also OMIM:100100

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi276E → A: Loss of basolateral sorting. 1 Publication1
Mutagenesisi276E → D: Loss of basolateral sorting. No effect on basolateral sorting; when associated with L-280 and L-281. 1 Publication1
Mutagenesisi280F → A: Loss of basolateral sorting. 1 Publication1
Mutagenesisi280F → L: No effect on basolateral sorting. 1 Publication1
Mutagenesisi281V → A: Loss of basolateral sorting. 1 Publication1
Mutagenesisi281V → L: No effect on basolateral sorting. 1 Publication1

Organism-specific databases

DisGeNETi1131.
MalaCardsiCHRM3.
MIMi100100. phenotype.
OpenTargetsiENSG00000133019.
Orphaneti2970. Prune belly syndrome.
PharmGKBiPA112.

Chemistry databases

ChEMBLiCHEMBL245.
DrugBankiDB08897. Aclidinium.
DB00321. Amitriptyline.
DB00543. Amoxapine.
DB00517. Anisotropine Methylbromide.
DB01238. Aripiprazole.
DB00572. Atropine.
DB00835. Brompheniramine.
DB00185. Cevimeline.
DB00477. Chlorpromazine.
DB01239. Chlorprothixene.
DB00568. Cinnarizine.
DB00363. Clozapine.
DB00785. Cryptenamine.
DB00434. Cyproheptadine.
DB00496. Darifenacin.
DB01151. Desipramine.
DB00729. Diphemanil Methylsulfate.
DB01231. Diphenidol.
DB00280. Disopyramide.
DB01142. Doxepin.
DB06702. Fesoterodine.
DB00986. Glycopyrrolate.
DB00725. Homatropine Methylbromide.
DB00424. Hyoscyamine.
DB00458. Imipramine.
DB00332. Ipratropium bromide.
DB01625. Isopropamide.
DB01221. Ketamine.
DB00408. Loxapine.
DB00934. Maprotiline.
DB04843. Mepenzolate.
DB06709. Methacholine.
DB01403. Methotrimeprazine.
DB00462. Methylscopolamine bromide.
DB00340. Metixene.
DB01226. Mivacurium.
DB00622. Nicardipine.
DB00540. Nortriptyline.
DB00334. Olanzapine.
DB01062. Oxybutynin.
DB00383. Oxyphencyclimine.
DB01337. Pancuronium.
DB00715. Paroxetine.
DB00454. Pethidine.
DB01085. Pilocarpine.
DB01338. Pipecuronium.
DB00387. Procyclidine.
DB00420. Promazine.
DB01069. Promethazine.
DB00777. Propiomazine.
DB01224. Quetiapine.
DB00747. Scopolamine.
DB01591. Solifenacin.
DB00202. Succinylcholine.
DB01409. Tiotropium.
DB01036. Tolterodine.
DB00193. Tramadol.
DB00376. Trihexyphenidyl.
DB00726. Trimipramine.
DB00809. Tropicamide.
DB09076. Umeclidinium.
DB00246. Ziprasidone.
GuidetoPHARMACOLOGYi15.

Polymorphism and mutation databases

BioMutaiCHRM3.
DMDMi113125.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000690291 – 590Muscarinic acetylcholine receptor M3Add BLAST590

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi5N-linked (GlcNAc...)Sequence analysis1
Glycosylationi6N-linked (GlcNAc...)Sequence analysis1
Glycosylationi15N-linked (GlcNAc...)Sequence analysis1
Glycosylationi41N-linked (GlcNAc...)Sequence analysis1
Glycosylationi48N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi141 ↔ 221PROSITE-ProRule annotation
Modified residuei385PhosphoserineBy similarity1
Disulfide bondi517 ↔ 520PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP20309.
PaxDbiP20309.
PeptideAtlasiP20309.
PRIDEiP20309.

PTM databases

iPTMnetiP20309.
PhosphoSitePlusiP20309.

Expressioni

Gene expression databases

BgeeiENSG00000133019.
CleanExiHS_CHRM3.
ExpressionAtlasiP20309. baseline and differential.
GenevisibleiP20309. HS.

Organism-specific databases

HPAiCAB010409.
HPA024106.
HPA048036.

Interactioni

Subunit structurei

Homodimer; the dimers can form tetramers (PubMed:23521066). Interacts with NALCN (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NALCNQ8IZF03EBI-2687785,EBI-7085333

Protein-protein interaction databases

BioGridi107553. 50 interactors.
DIPiDIP-44291N.
IntActiP20309. 3 interactors.
MINTiMINT-4953628.
STRINGi9606.ENSP00000255380.

Chemistry databases

BindingDBiP20309.

Structurei

Secondary structure

1590
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi280 – 283Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CSANMR-A271-289[»]
ProteinModelPortaliP20309.
SMRiP20309.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20309.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni148 – 152Agonist bindingBy similarity5
Regioni507 – 530Agonist bindingBy similarityAdd BLAST24

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi275 – 281Basolateral sorting signal7

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP20309.
KOiK04131.
OMAiVNTFCNC.
OrthoDBiEOG091G06VI.
PhylomeDBiP20309.
TreeFamiTF320495.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001183. Musac_Ach_M3_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF61. PTHR24249:SF61. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00540. MUSCRINICM3R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20309-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLHNNSTTS PLFPNISSSW IHSPSDAGLP PGTVTHFGSY NVSRAAGNFS
60 70 80 90 100
SPDGTTDDPL GGHTVWQVVF IAFLTGILAL VTIIGNILVI VSFKVNKQLK
110 120 130 140 150
TVNNYFLLSL ACADLIIGVI SMNLFTTYII MNRWALGNLA CDLWLAIDYV
160 170 180 190 200
ASNASVMNLL VISFDRYFSI TRPLTYRAKR TTKRAGVMIG LAWVISFVLW
210 220 230 240 250
APAILFWQYF VGKRTVPPGE CFIQFLSEPT ITFGTAIAAF YMPVTIMTIL
260 270 280 290 300
YWRIYKETEK RTKELAGLQA SGTEAETENF VHPTGSSRSC SSYELQQQSM
310 320 330 340 350
KRSNRRKYGR CHFWFTTKSW KPSSEQMDQD HSSSDSWNNN DAAASLENSA
360 370 380 390 400
SSDEEDIGSE TRAIYSIVLK LPGHSTILNS TKLPSSDNLQ VPEEELGMVD
410 420 430 440 450
LERKADKLQA QKSVDDGGSF PKSFSKLPIQ LESAVDTAKT SDVNSSVGKS
460 470 480 490 500
TATLPLSFKE ATLAKRFALK TRSQITKRKR MSLVKEKKAA QTLSAILLAF
510 520 530 540 550
IITWTPYNIM VLVNTFCDSC IPKTFWNLGY WLCYINSTVN PVCYALCNKT
560 570 580 590
FRTTFKMLLL CQCDKKKRRK QQYQQRQSVI FHKRAPEQAL
Length:590
Mass (Da):66,128
Last modified:February 1, 1991 - v1
Checksum:i5CB473C57B9526E9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti382 – 384KLP → RLS in AAG30036 (PubMed:11238933).Curated3

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03346165V → I.Corresponds to variant rs2067481dbSNPEnsembl.1
Natural variantiVAR_049368431L → P.Corresponds to variant rs16839102dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15266 Genomic DNA. Translation: CAA33337.1.
U29589 Genomic DNA. Translation: AAA70337.1.
AB041395 Genomic DNA. Translation: BAA94480.1.
AF498917 mRNA. Translation: AAM18940.1.
AL356361 Genomic DNA. Translation: CAH72987.1.
BC096844 mRNA. Translation: AAH96844.1.
BC121026 mRNA. Translation: AAI21027.1.
AF279779 mRNA. Translation: AAG30036.1.
CCDSiCCDS1616.1.
PIRiS10128.
RefSeqiNP_000731.1. NM_000740.2.
XP_005273089.1. XM_005273032.3.
XP_011542343.1. XM_011544041.2.
XP_011542345.1. XM_011544043.2.
XP_011542346.1. XM_011544044.2.
XP_011542347.1. XM_011544045.2.
XP_011542348.1. XM_011544046.2.
XP_011542349.1. XM_011544047.2.
XP_016855641.1. XM_017000152.1.
XP_016855642.1. XM_017000153.1.
XP_016855643.1. XM_017000154.1.
XP_016855644.1. XM_017000155.1.
XP_016855645.1. XM_017000156.1.
XP_016855646.1. XM_017000157.1.
XP_016855647.1. XM_017000158.1.
XP_016855648.1. XM_017000159.1.
XP_016855649.1. XM_017000160.1.
XP_016855650.1. XM_017000161.1.
XP_016855651.1. XM_017000162.1.
XP_016855652.1. XM_017000163.1.
UniGeneiHs.155736.
Hs.7138.

Genome annotation databases

EnsembliENST00000255380; ENSP00000255380; ENSG00000133019.
ENST00000615928; ENSP00000482377; ENSG00000133019.
GeneIDi1131.
KEGGihsa:1131.
UCSCiuc001hyp.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15266 Genomic DNA. Translation: CAA33337.1.
U29589 Genomic DNA. Translation: AAA70337.1.
AB041395 Genomic DNA. Translation: BAA94480.1.
AF498917 mRNA. Translation: AAM18940.1.
AL356361 Genomic DNA. Translation: CAH72987.1.
BC096844 mRNA. Translation: AAH96844.1.
BC121026 mRNA. Translation: AAI21027.1.
AF279779 mRNA. Translation: AAG30036.1.
CCDSiCCDS1616.1.
PIRiS10128.
RefSeqiNP_000731.1. NM_000740.2.
XP_005273089.1. XM_005273032.3.
XP_011542343.1. XM_011544041.2.
XP_011542345.1. XM_011544043.2.
XP_011542346.1. XM_011544044.2.
XP_011542347.1. XM_011544045.2.
XP_011542348.1. XM_011544046.2.
XP_011542349.1. XM_011544047.2.
XP_016855641.1. XM_017000152.1.
XP_016855642.1. XM_017000153.1.
XP_016855643.1. XM_017000154.1.
XP_016855644.1. XM_017000155.1.
XP_016855645.1. XM_017000156.1.
XP_016855646.1. XM_017000157.1.
XP_016855647.1. XM_017000158.1.
XP_016855648.1. XM_017000159.1.
XP_016855649.1. XM_017000160.1.
XP_016855650.1. XM_017000161.1.
XP_016855651.1. XM_017000162.1.
XP_016855652.1. XM_017000163.1.
UniGeneiHs.155736.
Hs.7138.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CSANMR-A271-289[»]
ProteinModelPortaliP20309.
SMRiP20309.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107553. 50 interactors.
DIPiDIP-44291N.
IntActiP20309. 3 interactors.
MINTiMINT-4953628.
STRINGi9606.ENSP00000255380.

Chemistry databases

BindingDBiP20309.
ChEMBLiCHEMBL245.
DrugBankiDB08897. Aclidinium.
DB00321. Amitriptyline.
DB00543. Amoxapine.
DB00517. Anisotropine Methylbromide.
DB01238. Aripiprazole.
DB00572. Atropine.
DB00835. Brompheniramine.
DB00185. Cevimeline.
DB00477. Chlorpromazine.
DB01239. Chlorprothixene.
DB00568. Cinnarizine.
DB00363. Clozapine.
DB00785. Cryptenamine.
DB00434. Cyproheptadine.
DB00496. Darifenacin.
DB01151. Desipramine.
DB00729. Diphemanil Methylsulfate.
DB01231. Diphenidol.
DB00280. Disopyramide.
DB01142. Doxepin.
DB06702. Fesoterodine.
DB00986. Glycopyrrolate.
DB00725. Homatropine Methylbromide.
DB00424. Hyoscyamine.
DB00458. Imipramine.
DB00332. Ipratropium bromide.
DB01625. Isopropamide.
DB01221. Ketamine.
DB00408. Loxapine.
DB00934. Maprotiline.
DB04843. Mepenzolate.
DB06709. Methacholine.
DB01403. Methotrimeprazine.
DB00462. Methylscopolamine bromide.
DB00340. Metixene.
DB01226. Mivacurium.
DB00622. Nicardipine.
DB00540. Nortriptyline.
DB00334. Olanzapine.
DB01062. Oxybutynin.
DB00383. Oxyphencyclimine.
DB01337. Pancuronium.
DB00715. Paroxetine.
DB00454. Pethidine.
DB01085. Pilocarpine.
DB01338. Pipecuronium.
DB00387. Procyclidine.
DB00420. Promazine.
DB01069. Promethazine.
DB00777. Propiomazine.
DB01224. Quetiapine.
DB00747. Scopolamine.
DB01591. Solifenacin.
DB00202. Succinylcholine.
DB01409. Tiotropium.
DB01036. Tolterodine.
DB00193. Tramadol.
DB00376. Trihexyphenidyl.
DB00726. Trimipramine.
DB00809. Tropicamide.
DB09076. Umeclidinium.
DB00246. Ziprasidone.
GuidetoPHARMACOLOGYi15.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP20309.
PhosphoSitePlusiP20309.

Polymorphism and mutation databases

BioMutaiCHRM3.
DMDMi113125.

Proteomic databases

MaxQBiP20309.
PaxDbiP20309.
PeptideAtlasiP20309.
PRIDEiP20309.

Protocols and materials databases

DNASUi1131.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000255380; ENSP00000255380; ENSG00000133019.
ENST00000615928; ENSP00000482377; ENSG00000133019.
GeneIDi1131.
KEGGihsa:1131.
UCSCiuc001hyp.4. human.

Organism-specific databases

CTDi1131.
DisGeNETi1131.
GeneCardsiCHRM3.
HGNCiHGNC:1952. CHRM3.
HPAiCAB010409.
HPA024106.
HPA048036.
MalaCardsiCHRM3.
MIMi100100. phenotype.
118494. gene.
neXtProtiNX_P20309.
OpenTargetsiENSG00000133019.
Orphaneti2970. Prune belly syndrome.
PharmGKBiPA112.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP20309.
KOiK04131.
OMAiVNTFCNC.
OrthoDBiEOG091G06VI.
PhylomeDBiP20309.
TreeFamiTF320495.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000133019-MONOMER.
ReactomeiR-HSA-390648. Muscarinic acetylcholine receptors.
R-HSA-399997. Acetylcholine regulates insulin secretion.
R-HSA-416476. G alpha (q) signalling events.
SignaLinkiP20309.

Miscellaneous databases

ChiTaRSiCHRM3. human.
EvolutionaryTraceiP20309.
GeneWikiiMuscarinic_acetylcholine_receptor_M3.
GenomeRNAii1131.
PROiP20309.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000133019.
CleanExiHS_CHRM3.
ExpressionAtlasiP20309. baseline and differential.
GenevisibleiP20309. HS.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001183. Musac_Ach_M3_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF61. PTHR24249:SF61. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00540. MUSCRINICM3R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACM3_HUMAN
AccessioniPrimary (citable) accession number: P20309
Secondary accession number(s): Q0VAJ8
, Q4QRI3, Q5VXY2, Q9HB60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 2, 2016
This is version 171 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.