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Protein

Muscarinic acetylcholine receptor M3

Gene

CHRM3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.1 Publication

GO - Molecular functioni

  • acetylcholine binding Source: UniProtKB
  • G-protein coupled acetylcholine receptor activity Source: UniProtKB
  • phosphatidylinositol phospholipase C activity Source: ProtInc
  • signaling receptor activity Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-HSA-390648 Muscarinic acetylcholine receptors
R-HSA-399997 Acetylcholine regulates insulin secretion
R-HSA-416476 G alpha (q) signalling events
SignaLinkiP20309

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M3
Gene namesi
Name:CHRM3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000133019.11
HGNCiHGNC:1952 CHRM3
MIMi118494 gene
neXtProtiNX_P20309

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 67ExtracellularBy similarityAdd BLAST67
Transmembranei68 – 91Helical; Name=1By similarityAdd BLAST24
Topological domaini92 – 104CytoplasmicBy similarityAdd BLAST13
Transmembranei105 – 130Helical; Name=2By similarityAdd BLAST26
Topological domaini131 – 142ExtracellularBy similarityAdd BLAST12
Transmembranei143 – 164Helical; Name=3By similarityAdd BLAST22
Topological domaini165 – 184CytoplasmicBy similarityAdd BLAST20
Transmembranei185 – 206Helical; Name=4By similarityAdd BLAST22
Topological domaini207 – 229ExtracellularBy similarityAdd BLAST23
Transmembranei230 – 252Helical; Name=5By similarityAdd BLAST23
Topological domaini253 – 491CytoplasmicBy similarityAdd BLAST239
Transmembranei492 – 514Helical; Name=6By similarityAdd BLAST23
Topological domaini515 – 526ExtracellularBy similarityAdd BLAST12
Transmembranei527 – 546Helical; Name=7By similarityAdd BLAST20
Topological domaini547 – 590CytoplasmicBy similarityAdd BLAST44

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Involvement in diseasei

Prune belly syndrome (PBS)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities.
See also OMIM:100100

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi276E → A: Loss of basolateral sorting. 1 Publication1
Mutagenesisi276E → D: Loss of basolateral sorting. No effect on basolateral sorting; when associated with L-280 and L-281. 1 Publication1
Mutagenesisi280F → A: Loss of basolateral sorting. 1 Publication1
Mutagenesisi280F → L: No effect on basolateral sorting. 1 Publication1
Mutagenesisi281V → A: Loss of basolateral sorting. 1 Publication1
Mutagenesisi281V → L: No effect on basolateral sorting. 1 Publication1

Organism-specific databases

DisGeNETi1131
MalaCardsiCHRM3
MIMi100100 phenotype
OpenTargetsiENSG00000133019
Orphaneti2970 Prune belly syndrome
PharmGKBiPA112

Chemistry databases

ChEMBLiCHEMBL245
DrugBankiDB08897 Aclidinium
DB05752 ALKS 27
DB00321 Amitriptyline
DB00543 Amoxapine
DB00517 Anisotropine Methylbromide
DB01238 Aripiprazole
DB00572 Atropine
DB00767 Benzquinamide
DB00835 Brompheniramine
DB00185 Cevimeline
DB00477 Chlorpromazine
DB01239 Chlorprothixene
DB00568 Cinnarizine
DB00363 Clozapine
DB00785 Cryptenamine
DB00434 Cyproheptadine
DB00496 Darifenacin
DB01151 Desipramine
DB09357 Dexpanthenol
DB00729 Diphemanil Methylsulfate
DB01231 Diphenidol
DB00280 Disopyramide
DB01142 Doxepin
DB06702 Fesoterodine
DB00986 Glycopyrronium
DB00725 Homatropine Methylbromide
DB00424 Hyoscyamine
DB00458 Imipramine
DB00332 Ipratropium bromide
DB01625 Isopropamide
DB04924 Itopride
DB01221 Ketamine
DB00408 Loxapine
DB00934 Maprotiline
DB04843 Mepenzolate
DB06709 Methacholine
DB01403 Methotrimeprazine
DB00462 Methylscopolamine bromide
DB00340 Metixene
DB01226 Mivacurium
DB00622 Nicardipine
DB00540 Nortriptyline
DB00334 Olanzapine
DB01062 Oxybutynin
DB00383 Oxyphencyclimine
DB01337 Pancuronium
DB00715 Paroxetine
DB00454 Pethidine
DB01085 Pilocarpine
DB01338 Pipecuronium
DB00387 Procyclidine
DB00420 Promazine
DB01069 Promethazine
DB00777 Propiomazine
DB01224 Quetiapine
DB00747 Scopolamine
DB01591 Solifenacin
DB00202 Succinylcholine
DB00342 Terfenadine
DB01409 Tiotropium
DB01036 Tolterodine
DB00193 Tramadol
DB00505 Tridihexethyl
DB00376 Trihexyphenidyl
DB00726 Trimipramine
DB00809 Tropicamide
DB09076 Umeclidinium
DB00246 Ziprasidone
GuidetoPHARMACOLOGYi15

Polymorphism and mutation databases

BioMutaiCHRM3
DMDMi113125

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000690291 – 590Muscarinic acetylcholine receptor M3Add BLAST590

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi5N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi6N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi15N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi41N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi48N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi141 ↔ 221PROSITE-ProRule annotation
Modified residuei385PhosphoserineBy similarity1
Disulfide bondi517 ↔ 520PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP20309
PaxDbiP20309
PeptideAtlasiP20309
PRIDEiP20309

PTM databases

iPTMnetiP20309
PhosphoSitePlusiP20309

Expressioni

Gene expression databases

BgeeiENSG00000133019
CleanExiHS_CHRM3
ExpressionAtlasiP20309 baseline and differential
GenevisibleiP20309 HS

Organism-specific databases

HPAiHPA024106
HPA048036

Interactioni

Subunit structurei

Homodimer; the dimers can form tetramers (PubMed:23521066). Interacts with NALCN (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NALCNQ8IZF03EBI-2687785,EBI-7085333

Protein-protein interaction databases

BioGridi107553, 58 interactors
DIPiDIP-44291N
IntActiP20309, 4 interactors
MINTiP20309
STRINGi9606.ENSP00000255380

Chemistry databases

BindingDBiP20309

Structurei

Secondary structure

1590
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi280 – 283Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CSANMR-A271-289[»]
ProteinModelPortaliP20309
SMRiP20309
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20309

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni148 – 152Agonist bindingBy similarity5
Regioni507 – 530Agonist bindingBy similarityAdd BLAST24

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi275 – 281Basolateral sorting signal7

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4220 Eukaryota
ENOG410YCQR LUCA
GeneTreeiENSGT00780000121874
HOGENOMiHOG000231484
HOVERGENiHBG105720
InParanoidiP20309
KOiK04131
OMAiCQCDKRK
OrthoDBiEOG091G06VI
PhylomeDBiP20309
TreeFamiTF320495

Family and domain databases

CDDicd15299 7tmA_mAChR_M3, 1 hit
InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR001183 Musac_Ach_M3_rcpt
IPR000995 Musac_Ach_rcpt
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR00243 MUSCARINICR
PR00540 MUSCRINICM3R
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequencei

Sequence statusi: Complete.

P20309-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLHNNSTTS PLFPNISSSW IHSPSDAGLP PGTVTHFGSY NVSRAAGNFS
60 70 80 90 100
SPDGTTDDPL GGHTVWQVVF IAFLTGILAL VTIIGNILVI VSFKVNKQLK
110 120 130 140 150
TVNNYFLLSL ACADLIIGVI SMNLFTTYII MNRWALGNLA CDLWLAIDYV
160 170 180 190 200
ASNASVMNLL VISFDRYFSI TRPLTYRAKR TTKRAGVMIG LAWVISFVLW
210 220 230 240 250
APAILFWQYF VGKRTVPPGE CFIQFLSEPT ITFGTAIAAF YMPVTIMTIL
260 270 280 290 300
YWRIYKETEK RTKELAGLQA SGTEAETENF VHPTGSSRSC SSYELQQQSM
310 320 330 340 350
KRSNRRKYGR CHFWFTTKSW KPSSEQMDQD HSSSDSWNNN DAAASLENSA
360 370 380 390 400
SSDEEDIGSE TRAIYSIVLK LPGHSTILNS TKLPSSDNLQ VPEEELGMVD
410 420 430 440 450
LERKADKLQA QKSVDDGGSF PKSFSKLPIQ LESAVDTAKT SDVNSSVGKS
460 470 480 490 500
TATLPLSFKE ATLAKRFALK TRSQITKRKR MSLVKEKKAA QTLSAILLAF
510 520 530 540 550
IITWTPYNIM VLVNTFCDSC IPKTFWNLGY WLCYINSTVN PVCYALCNKT
560 570 580 590
FRTTFKMLLL CQCDKKKRRK QQYQQRQSVI FHKRAPEQAL
Length:590
Mass (Da):66,128
Last modified:February 1, 1991 - v1
Checksum:i5CB473C57B9526E9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti382 – 384KLP → RLS in AAG30036 (PubMed:11238933).Curated3

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03346165V → I. Corresponds to variant dbSNP:rs2067481Ensembl.1
Natural variantiVAR_049368431L → P. Corresponds to variant dbSNP:rs16839102Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15266 Genomic DNA Translation: CAA33337.1
U29589 Genomic DNA Translation: AAA70337.1
AB041395 Genomic DNA Translation: BAA94480.1
AF498917 mRNA Translation: AAM18940.1
AL356361 Genomic DNA No translation available.
BC096844 mRNA Translation: AAH96844.1
BC121026 mRNA Translation: AAI21027.1
AF279779 mRNA Translation: AAG30036.1
CCDSiCCDS1616.1
PIRiS10128
RefSeqiNP_000731.1, NM_000740.3
NP_001334645.1, NM_001347716.1
XP_005273089.1, XM_005273032.3
XP_011542343.1, XM_011544041.2
XP_011542345.1, XM_011544043.2
XP_011542346.1, XM_011544044.2
XP_011542348.1, XM_011544046.2
XP_011542349.1, XM_011544047.2
XP_016855641.1, XM_017000152.1
XP_016855642.1, XM_017000153.1
XP_016855643.1, XM_017000154.1
XP_016855644.1, XM_017000155.1
XP_016855645.1, XM_017000156.1
XP_016855646.1, XM_017000157.1
XP_016855647.1, XM_017000158.1
XP_016855648.1, XM_017000159.1
XP_016855649.1, XM_017000160.1
XP_016855650.1, XM_017000161.1
XP_016855651.1, XM_017000162.1
XP_016855652.1, XM_017000163.1
UniGeneiHs.155736
Hs.7138

Genome annotation databases

EnsembliENST00000255380; ENSP00000255380; ENSG00000133019
ENST00000615928; ENSP00000482377; ENSG00000133019
GeneIDi1131
KEGGihsa:1131
UCSCiuc001hyp.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiACM3_HUMAN
AccessioniPrimary (citable) accession number: P20309
Secondary accession number(s): Q0VAJ8
, Q4QRI3, Q5VXY2, Q9HB60
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: May 23, 2018
This is version 181 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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