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Protein

Brachyury protein

Gene

T

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi51 – 219T-boxPROSITE-ProRule annotationAdd BLAST169

GO - Molecular functioni

GO - Biological processi

  • anatomical structure morphogenesis Source: MGI
  • anterior/posterior pattern specification Source: MGI
  • BMP signaling pathway Source: MGI
  • bone morphogenesis Source: MGI
  • canonical Wnt signaling pathway Source: MGI
  • cardiac muscle cell differentiation Source: UniProtKB
  • cellular response to retinoic acid Source: Ensembl
  • determination of heart left/right asymmetry Source: MGI
  • embryonic skeletal system development Source: MGI
  • heart morphogenesis Source: MGI
  • mesoderm development Source: MGI
  • mesoderm migration involved in gastrulation Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • neural plate morphogenesis Source: MGI
  • neural tube closure Source: MGI
  • notochord development Source: MGI
  • notochord formation Source: MGI
  • penetration of zona pellucida Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation Source: BHF-UCL
  • post-anal tail morphogenesis Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • reproductive process Source: MGI
  • signal transduction involved in regulation of gene expression Source: MGI
  • SMAD protein signal transduction Source: MGI
  • somitogenesis Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • vasculogenesis Source: MGI

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Brachyury protein
Alternative name(s):
Protein T
Gene namesi
Name:T
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:98472. T.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

  • chromatin Source: BHF-UCL
  • cytoplasm Source: MGI
  • nuclear chromatin Source: MGI
  • nucleus Source: MGI

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Embryo lacking the T gene fail to form the notochord, the entire posterior region and the allantois, and die at about 10 days of gestation.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001844151 – 436Brachyury proteinAdd BLAST436

Proteomic databases

PaxDbiP20293.
PRIDEiP20293.

PTM databases

PhosphoSitePlusiP20293.

Expressioni

Developmental stagei

During gastrula and neurula stages in involuting mesoderm and in the notochord.

Gene expression databases

BgeeiENSMUSG00000062327.
CleanExiMM_T.
ExpressionAtlasiP20293. baseline and differential.
GenevisibleiP20293. MM.

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

WithEntry#Exp.IntActNotes
Mixl1Q9WUI07EBI-13637160,EBI-13637214

GO - Molecular functioni

  • RNA polymerase II activating transcription factor binding Source: BHF-UCL
  • RNA polymerase II transcription factor binding Source: BHF-UCL

Protein-protein interaction databases

DIPiDIP-61216N.
IntActiP20293. 2 interactors.
STRINGi10090.ENSMUSP00000074236.

Structurei

3D structure databases

ProteinModelPortaliP20293.
SMRiP20293.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiKOG3585. Eukaryota.
ENOG410XSTS. LUCA.
GeneTreeiENSGT00760000118897.
HOGENOMiHOG000063663.
HOVERGENiHBG004599.
InParanoidiP20293.
KOiK10172.
OMAiAPSCVYI.
OrthoDBiEOG091G05OC.
PhylomeDBiP20293.
TreeFamiTF106341.

Family and domain databases

CDDicd00182. TBOX. 1 hit.
Gene3Di2.60.40.820. 1 hit.
InterProiView protein in InterPro
IPR008967. p53-like_TF_DNA-bd.
IPR002070. TF_Brachyury.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
PANTHERiPTHR11267. PTHR11267. 1 hit.
PfamiView protein in Pfam
PF00907. T-box. 1 hit.
PRINTSiPR00938. BRACHYURY.
PR00937. TBOX.
SMARTiView protein in SMART
SM00425. TBOX. 1 hit.
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiView protein in PROSITE
PS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.

Sequencei

Sequence statusi: Complete.

P20293-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPGTESAG KSLQYRVDHL LSAVESELQA GSEKGDPTER ELRVGLEESE
60 70 80 90 100
LWLRFKELTN EMIVTKNGRR MFPVLKVNVS GLDPNAMYSF LLDFVTADNH
110 120 130 140 150
RWKYVNGEWV PGGKPEPQAP SCVYIHPDSP NFGAHWMKAP VSFSKVKLTN
160 170 180 190 200
KLNGGGQIML NSLHKYEPRI HIVRVGGPQR MITSHCFPET QFIAVTAYQN
210 220 230 240 250
EEITALKIKY NPFAKAFLDA KERNDHKDVM EEPGDCQQPG YSQWGWLVPG
260 270 280 290 300
AGTLCPPASS HPQFGGSLSL PSTHGCERYP ALRNHRSSPY PSPYAHRNSS
310 320 330 340 350
PTYADNSSAC LSMLQSHDNW SSLGVPGHTS MLPVSHNASP PTGSSQYPSL
360 370 380 390 400
WSVSNGTITP GSQTAGVSNG LGAQFFRGSP AHYTPLTHTV SAATSSSSGS
410 420 430
PMYEGAATVT DISDSQYDTA QSLLIASWTP VSPPSM
Length:436
Mass (Da):47,440
Last modified:February 1, 1991 - v1
Checksum:i1A0BDF9BA7FC2484
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51683 mRNA. Translation: CAA35985.1.
CCDSiCCDS28383.1.
PIRiS08156.
RefSeqiNP_033335.1. NM_009309.2.
XP_011244504.1. XM_011246202.1.
UniGeneiMm.913.

Genome annotation databases

EnsembliENSMUST00000074667; ENSMUSP00000074236; ENSMUSG00000062327.
GeneIDi20997.
KEGGimmu:20997.
UCSCiuc008ajq.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiBRAC_MOUSE
AccessioniPrimary (citable) accession number: P20293
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: June 7, 2017
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot