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P20291

- AL5AP_RAT

UniProt

P20291 - AL5AP_RAT

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Protein

Arachidonate 5-lipoxygenase-activating protein

Gene

Alox5ap

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes. unstructured (By similarity).By similarity

GO - Molecular functioni

  1. arachidonic acid binding Source: UniProtKB
  2. enzyme activator activity Source: InterPro
  3. enzyme binding Source: RGD
  4. protein heterodimerization activity Source: RGD
  5. protein homodimerization activity Source: RGD

GO - Biological processi

  1. cellular response to calcium ion Source: Ensembl
  2. leukotriene biosynthetic process Source: RGD
  3. leukotriene production involved in inflammatory response Source: Ensembl
  4. lipoxygenase pathway Source: RGD
  5. oxidation-reduction process Source: GOC
  6. positive regulation of acute inflammatory response Source: RGD
  7. protein homotrimerization Source: RGD
Complete GO annotation...

Keywords - Biological processi

Leukotriene biosynthesis

Enzyme and pathway databases

ReactomeiREACT_197237. Synthesis of Leukotrienes (LT) and Eoxins (EX).
REACT_197256. Synthesis of 5-eicosatetraenoic acids.
REACT_198834. Synthesis of Lipoxins (LX).

Names & Taxonomyi

Protein namesi
Recommended name:
Arachidonate 5-lipoxygenase-activating protein
Alternative name(s):
FLAP
MK-886-binding protein
Gene namesi
Name:Alox5ap
Synonyms:Flap
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 12

Organism-specific databases

RGDi2097. Alox5ap.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88LumenalBy similarity
Transmembranei9 – 3022HelicalBy similarityAdd
BLAST
Topological domaini31 – 5222CytoplasmicBy similarityAdd
BLAST
Transmembranei53 – 7725HelicalBy similarityAdd
BLAST
Topological domaini78 – 803LumenalBy similarity
Transmembranei81 – 10222HelicalBy similarityAdd
BLAST
Topological domaini103 – 1075CytoplasmicBy similarity
Intramembranei108 – 1158By similarity
Transmembranei116 – 12813HelicalBy similarityAdd
BLAST
Topological domaini129 – 16133LumenalBy similarityAdd
BLAST

GO - Cellular componenti

  1. cytosol Source: RGD
  2. endoplasmic reticulum Source: RGD
  3. integral component of membrane Source: UniProtKB-KW
  4. membrane Source: RGD
  5. nuclear envelope Source: UniProtKB
  6. nuclear membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 161161Arachidonate 5-lipoxygenase-activating proteinPRO_0000217756Add
BLAST

Proteomic databases

PRIDEiP20291.

PTM databases

PhosphoSiteiP20291.

Expressioni

Gene expression databases

GenevestigatoriP20291.

Interactioni

Subunit structurei

Homotrimer. Interacts with LTC4S and ALOX5 (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-48660N.
STRINGi10116.ENSRNOP00000001207.

Structurei

3D structure databases

ProteinModelPortaliP20291.
SMRiP20291. Positions 1-149.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni20 – 278Inhibitor bindingBy similarity
Regioni112 – 12312Inhibitor bindingBy similarityAdd
BLAST

Domaini

The C-terminal part after residue 140 is mostly disordered.By similarity

Sequence similaritiesi

Belongs to the MAPEG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG79474.
GeneTreeiENSGT00430000030964.
HOGENOMiHOG000116372.
HOVERGENiHBG107295.
InParanoidiP20291.
OMAiNCMDAYP.
OrthoDBiEOG7GXPD5.
PhylomeDBiP20291.
TreeFamiTF105328.

Family and domain databases

Gene3Di1.20.120.550. 1 hit.
InterProiIPR001446. 5_LipOase_AP.
IPR018295. FLAP/GST2/LTC4S_CS.
IPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamiPF01124. MAPEG. 1 hit.
[Graphical view]
PRINTSiPR00488. 5LPOXGNASEAP.
PROSITEiPS01297. FLAP_GST2_LTC4S. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20291-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDQEAVGNVV LLAIVTLISV VQNAFFAHKV ELESKAQSGR SFQRTGTLAF
60 70 80 90 100
ERVYTANQNC VDAYPTFLVV LWTAGLLCSQ VPAAFAGLMY LFVRQKYFVG
110 120 130 140 150
YLGERTQSTP GYIFGKRIIL FLFLMSLAGI LNHYLIFFFG SDFENYIRTI
160
TTTISPLLLI P
Length:161
Mass (Da):18,070
Last modified:May 1, 2007 - v2
Checksum:i9CCD6F8B3AA3B87E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti32 – 321L → I AA sequence (PubMed:2300172)Curated
Sequence conflicti127 – 1271L → I AA sequence (PubMed:2300172)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52196 mRNA. Translation: CAA36442.1.
BC086593 mRNA. Translation: AAH86593.1.
PIRiS08206.
RefSeqiNP_058956.1. NM_017260.2.
UniGeneiRn.18399.

Genome annotation databases

EnsembliENSRNOT00000001207; ENSRNOP00000001207; ENSRNOG00000000907.
GeneIDi29624.
KEGGirno:29624.
UCSCiRGD:2097. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52196 mRNA. Translation: CAA36442.1 .
BC086593 mRNA. Translation: AAH86593.1 .
PIRi S08206.
RefSeqi NP_058956.1. NM_017260.2.
UniGenei Rn.18399.

3D structure databases

ProteinModelPortali P20291.
SMRi P20291. Positions 1-149.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48660N.
STRINGi 10116.ENSRNOP00000001207.

Chemistry

BindingDBi P20291.
ChEMBLi CHEMBL1921661.

PTM databases

PhosphoSitei P20291.

Proteomic databases

PRIDEi P20291.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000001207 ; ENSRNOP00000001207 ; ENSRNOG00000000907 .
GeneIDi 29624.
KEGGi rno:29624.
UCSCi RGD:2097. rat.

Organism-specific databases

CTDi 241.
RGDi 2097. Alox5ap.

Phylogenomic databases

eggNOGi NOG79474.
GeneTreei ENSGT00430000030964.
HOGENOMi HOG000116372.
HOVERGENi HBG107295.
InParanoidi P20291.
OMAi NCMDAYP.
OrthoDBi EOG7GXPD5.
PhylomeDBi P20291.
TreeFami TF105328.

Enzyme and pathway databases

Reactomei REACT_197237. Synthesis of Leukotrienes (LT) and Eoxins (EX).
REACT_197256. Synthesis of 5-eicosatetraenoic acids.
REACT_198834. Synthesis of Lipoxins (LX).

Miscellaneous databases

NextBioi 609834.
PROi P20291.

Gene expression databases

Genevestigatori P20291.

Family and domain databases

Gene3Di 1.20.120.550. 1 hit.
InterProi IPR001446. 5_LipOase_AP.
IPR018295. FLAP/GST2/LTC4S_CS.
IPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view ]
Pfami PF01124. MAPEG. 1 hit.
[Graphical view ]
PRINTSi PR00488. 5LPOXGNASEAP.
PROSITEi PS01297. FLAP_GST2_LTC4S. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Requirement of a 5-lipoxygenase-activating protein for leukotriene synthesis."
    Dixon R.A.F., Diehl R.E., Opas E., Rands E., Vickers P.J., Evans J.F., Gillard J.W., Miller D.K.
    Nature 343:282-284(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.
  3. Cited for: PROTEIN SEQUENCE OF 1-39; 90-121 AND 126-146.

Entry informationi

Entry nameiAL5AP_RAT
AccessioniPrimary (citable) accession number: P20291
Secondary accession number(s): Q5RJL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: May 1, 2007
Last modified: October 29, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3