Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P20291

- AL5AP_RAT

UniProt

P20291 - AL5AP_RAT

Protein

Arachidonate 5-lipoxygenase-activating protein

Gene

Alox5ap

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 2 (01 May 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes. unstructured By similarity.By similarity

    GO - Molecular functioni

    1. arachidonic acid binding Source: UniProtKB
    2. enzyme activator activity Source: InterPro
    3. enzyme binding Source: RGD
    4. protein heterodimerization activity Source: RGD
    5. protein homodimerization activity Source: RGD

    GO - Biological processi

    1. cellular response to calcium ion Source: Ensembl
    2. leukotriene biosynthetic process Source: RGD
    3. leukotriene production involved in inflammatory response Source: Ensembl
    4. lipoxygenase pathway Source: RGD
    5. oxidation-reduction process Source: GOC
    6. positive regulation of acute inflammatory response Source: RGD
    7. protein homotrimerization Source: RGD

    Keywords - Biological processi

    Leukotriene biosynthesis

    Enzyme and pathway databases

    ReactomeiREACT_197237. Synthesis of Leukotrienes (LT) and Eoxins (EX).
    REACT_197256. Synthesis of 5-eicosatetraenoic acids.
    REACT_198834. Synthesis of Lipoxins (LX).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arachidonate 5-lipoxygenase-activating protein
    Alternative name(s):
    FLAP
    MK-886-binding protein
    Gene namesi
    Name:Alox5ap
    Synonyms:Flap
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 12

    Organism-specific databases

    RGDi2097. Alox5ap.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: RGD
    2. endoplasmic reticulum Source: RGD
    3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB-KW
    5. membrane Source: RGD
    6. nuclear envelope Source: UniProtKB
    7. nuclear membrane Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 161161Arachidonate 5-lipoxygenase-activating proteinPRO_0000217756Add
    BLAST

    Proteomic databases

    PRIDEiP20291.

    PTM databases

    PhosphoSiteiP20291.

    Expressioni

    Gene expression databases

    GenevestigatoriP20291.

    Interactioni

    Subunit structurei

    Homotrimer. Interacts with LTC4S and ALOX5 By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-48660N.
    STRINGi10116.ENSRNOP00000001207.

    Structurei

    3D structure databases

    ProteinModelPortaliP20291.
    SMRiP20291. Positions 1-149.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 88LumenalBy similarity
    Topological domaini31 – 5222CytoplasmicBy similarityAdd
    BLAST
    Topological domaini78 – 803LumenalBy similarity
    Topological domaini103 – 1075CytoplasmicBy similarity
    Topological domaini129 – 16133LumenalBy similarityAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei108 – 1158By similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei9 – 3022HelicalBy similarityAdd
    BLAST
    Transmembranei53 – 7725HelicalBy similarityAdd
    BLAST
    Transmembranei81 – 10222HelicalBy similarityAdd
    BLAST
    Transmembranei116 – 12813HelicalBy similarityAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni20 – 278Inhibitor bindingBy similarity
    Regioni112 – 12312Inhibitor bindingBy similarityAdd
    BLAST

    Domaini

    The C-terminal part after residue 140 is mostly disordered.By similarity

    Sequence similaritiesi

    Belongs to the MAPEG family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG79474.
    GeneTreeiENSGT00430000030964.
    HOGENOMiHOG000116372.
    HOVERGENiHBG107295.
    InParanoidiP20291.
    OMAiNCMDAYP.
    OrthoDBiEOG7GXPD5.
    PhylomeDBiP20291.
    TreeFamiTF105328.

    Family and domain databases

    Gene3Di1.20.120.550. 1 hit.
    InterProiIPR001446. 5_LipOase_AP.
    IPR018295. FLAP/GST2/LTC4S_CS.
    IPR023352. MAPEG-like_dom.
    IPR001129. Membr-assoc_MAPEG.
    [Graphical view]
    PfamiPF01124. MAPEG. 1 hit.
    [Graphical view]
    PRINTSiPR00488. 5LPOXGNASEAP.
    PROSITEiPS01297. FLAP_GST2_LTC4S. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P20291-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDQEAVGNVV LLAIVTLISV VQNAFFAHKV ELESKAQSGR SFQRTGTLAF    50
    ERVYTANQNC VDAYPTFLVV LWTAGLLCSQ VPAAFAGLMY LFVRQKYFVG 100
    YLGERTQSTP GYIFGKRIIL FLFLMSLAGI LNHYLIFFFG SDFENYIRTI 150
    TTTISPLLLI P 161
    Length:161
    Mass (Da):18,070
    Last modified:May 1, 2007 - v2
    Checksum:i9CCD6F8B3AA3B87E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti32 – 321L → I AA sequence (PubMed:2300172)Curated
    Sequence conflicti127 – 1271L → I AA sequence (PubMed:2300172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52196 mRNA. Translation: CAA36442.1.
    BC086593 mRNA. Translation: AAH86593.1.
    PIRiS08206.
    RefSeqiNP_058956.1. NM_017260.2.
    UniGeneiRn.18399.

    Genome annotation databases

    EnsembliENSRNOT00000001207; ENSRNOP00000001207; ENSRNOG00000000907.
    GeneIDi29624.
    KEGGirno:29624.
    UCSCiRGD:2097. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52196 mRNA. Translation: CAA36442.1 .
    BC086593 mRNA. Translation: AAH86593.1 .
    PIRi S08206.
    RefSeqi NP_058956.1. NM_017260.2.
    UniGenei Rn.18399.

    3D structure databases

    ProteinModelPortali P20291.
    SMRi P20291. Positions 1-149.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48660N.
    STRINGi 10116.ENSRNOP00000001207.

    Chemistry

    BindingDBi P20291.
    ChEMBLi CHEMBL1921661.

    PTM databases

    PhosphoSitei P20291.

    Proteomic databases

    PRIDEi P20291.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000001207 ; ENSRNOP00000001207 ; ENSRNOG00000000907 .
    GeneIDi 29624.
    KEGGi rno:29624.
    UCSCi RGD:2097. rat.

    Organism-specific databases

    CTDi 241.
    RGDi 2097. Alox5ap.

    Phylogenomic databases

    eggNOGi NOG79474.
    GeneTreei ENSGT00430000030964.
    HOGENOMi HOG000116372.
    HOVERGENi HBG107295.
    InParanoidi P20291.
    OMAi NCMDAYP.
    OrthoDBi EOG7GXPD5.
    PhylomeDBi P20291.
    TreeFami TF105328.

    Enzyme and pathway databases

    Reactomei REACT_197237. Synthesis of Leukotrienes (LT) and Eoxins (EX).
    REACT_197256. Synthesis of 5-eicosatetraenoic acids.
    REACT_198834. Synthesis of Lipoxins (LX).

    Miscellaneous databases

    NextBioi 609834.
    PROi P20291.

    Gene expression databases

    Genevestigatori P20291.

    Family and domain databases

    Gene3Di 1.20.120.550. 1 hit.
    InterProi IPR001446. 5_LipOase_AP.
    IPR018295. FLAP/GST2/LTC4S_CS.
    IPR023352. MAPEG-like_dom.
    IPR001129. Membr-assoc_MAPEG.
    [Graphical view ]
    Pfami PF01124. MAPEG. 1 hit.
    [Graphical view ]
    PRINTSi PR00488. 5LPOXGNASEAP.
    PROSITEi PS01297. FLAP_GST2_LTC4S. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Requirement of a 5-lipoxygenase-activating protein for leukotriene synthesis."
      Dixon R.A.F., Diehl R.E., Opas E., Rands E., Vickers P.J., Evans J.F., Gillard J.W., Miller D.K.
      Nature 343:282-284(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary.
    3. Cited for: PROTEIN SEQUENCE OF 1-39; 90-121 AND 126-146.

    Entry informationi

    Entry nameiAL5AP_RAT
    AccessioniPrimary (citable) accession number: P20291
    Secondary accession number(s): Q5RJL3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 106 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3