P20291 (AL5AP_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arachidonate 5-lipoxygenase-activating protein

Alternative name(s):
FLAP
MK-886-binding protein

Gene names

Name:	Alox5ap
Synonyms:	Flap

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes. unstructured By similarity.
Subunit structure	Homotrimer. Interacts with LTC4S and ALOX5 By similarity.
Subcellular location	Nucleus membrane; Multi-pass membrane protein By similarity. Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Domain	The C-terminal part after residue 140 is mostly disordered By similarity.
Sequence similarities	Belongs to the MAPEG family.

Ontologies

Keywords
Biological process	Leukotriene biosynthesis
Cellular component	Endoplasmic reticulum Membrane Nucleus
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to calcium ion Inferred from electronic annotation. Source: Compara glutathione biosynthetic process Inferred from Biological aspect of Ancestor. Source: RefGenome leukotriene biosynthetic process Inferred from direct assay PubMed 19075240Ref.1. Source: RGD leukotriene production involved in inflammatory response Inferred from electronic annotation. Source: Compara lipoxygenase pathway Inferred from direct assay PubMed 17517102. Source: RGD positive regulation of acute inflammatory response Inferred from mutant phenotype PubMed 20519143. Source: RGD positive regulation of catalytic activity Inferred from electronic annotation. Source: GOC protein homotrimerization Inferred from direct assay PubMed 15084748. Source: RGD
Cellular_component	cytosol Inferred from direct assay PubMed 8071328. Source: RGD endoplasmic reticulum Inferred from direct assay PubMed 8071328. Source: RGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	arachidonic acid binding Inferred from sequence or structural similarity. Source: UniProtKB enzyme activator activity Inferred from electronic annotation. Source: InterPro glutathione peroxidase activity Inferred from Biological aspect of Ancestor. Source: RefGenome glutathione transferase activity Inferred from Biological aspect of Ancestor. Source: RefGenome protein homodimerization activity Inferred from direct assay PubMed 15084748. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 161	161	Arachidonate 5-lipoxygenase-activating protein		PRO_0000217756
Regions
	Topological domain	1 – 8	8	Lumenal By similarity
	Transmembrane	9 – 30	22	Helical; By similarity
	Topological domain	31 – 52	22	Cytoplasmic By similarity
	Transmembrane	53 – 77	25	Helical; By similarity
	Topological domain	78 – 80	3	Lumenal By similarity
	Transmembrane	81 – 102	22	Helical; By similarity
	Topological domain	103 – 107	5	Cytoplasmic By similarity
	Intramembrane	108 – 115	8	By similarity
	Transmembrane	116 – 128	13	Helical; By similarity
	Topological domain	129 – 161	33	Lumenal By similarity
	Region	20 – 27	8	Inhibitor binding By similarity
	Region	112 – 123	12	Inhibitor binding By similarity
Experimental info
	Sequence conflict	32	1	L → I AA sequence Ref.3
	Sequence conflict	127	1	L → I AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P20291 [UniParc]. Last modified May 1, 2007. Version 2. Checksum: 9CCD6F8B3AA3B87E	FASTA	161	18,070
	10 20 30 40 50 60 MDQEAVGNVV LLAIVTLISV VQNAFFAHKV ELESKAQSGR SFQRTGTLAF ERVYTANQNC 70 80 90 100 110 120 VDAYPTFLVV LWTAGLLCSQ VPAAFAGLMY LFVRQKYFVG YLGERTQSTP GYIFGKRIIL 130 140 150 160 FLFLMSLAGI LNHYLIFFFG SDFENYIRTI TTTISPLLLI P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Requirement of a 5-lipoxygenase-activating protein for leukotriene synthesis." Dixon R.A.F., Diehl R.E., Opas E., Rands E., Vickers P.J., Evans J.F., Gillard J.W., Miller D.K. Nature 343:282-284(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[3]	"Identification and isolation of a membrane protein necessary for leukotriene production." Miller D.K., Gillard J.W., Vickers P.J., Sadowski S., Leveille C., Mancini J.A., Charleson P., Dixon R.A.F., Ford-Hutchinson A.W., Fortin R., Gauthier J.Y., Rodkey J., Rosen R., Rouzer C., Sigal I.S., Strader C.D., Evans J.F. Nature 343:278-281(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-39; 90-121 AND 126-146.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52196 mRNA. Translation: CAA36442.1. BC086593 mRNA. Translation: AAH86593.1.
IPI	IPI00324979.
PIR	S08206.
RefSeq	NP_058956.1. NM_017260.2.
UniGene	Rn.18399.
3D structure databases
ProteinModelPortal	P20291.
SMR	P20291. Positions 1-149.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48660N.
STRING	10116.ENSRNOP00000001207.
PTM databases
PhosphoSite	P20291.
Proteomic databases
PRIDE	P20291.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000001207; ENSRNOP00000001207; ENSRNOG00000000907.
GeneID	29624.
KEGG	rno:29624.
UCSC	RGD:2097. rat.
Organism-specific databases
CTD	241.
RGD	2097. Alox5ap.
Phylogenomic databases
eggNOG	NOG79474.
GeneTree	ENSGT00430000030964.
HOGENOM	HOG000116372.
HOVERGEN	HBG107295.
InParanoid	P20291.
OMA	LVMLWSA.
OrthoDB	EOG4229KX.
Gene expression databases
Genevestigator	P20291.
GermOnline	ENSRNOG00000000907. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.120.550. 1 hit.
InterPro	IPR001446. 5_LipOase_AP. IPR018295. FLAP/GST2/LTC4S_CS. IPR023352. MAPEG-like_dom. IPR001129. Membr-assoc_MAPEG. [Graphical view]
Pfam	PF01124. MAPEG. 1 hit. [Graphical view]
PRINTS	PR00488. 5LPOXGNASEAP.
PROSITE	PS01297. FLAP_GST2_LTC4S. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P20291.
ChEMBL	CHEMBL1921661.
NextBio	609834.

Entry information

Entry name

AL5AP_RAT

Accession

Primary (citable) accession number: P20291
Secondary accession number(s): Q5RJL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	May 1, 2007
Last modified:	April 3, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program