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Protein

Arachidonate 5-lipoxygenase-activating protein

Gene

Alox5ap

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes (By similarity).By similarity

GO - Molecular functioni

  • arachidonic acid binding Source: UniProtKB
  • enzyme activator activity Source: InterPro
  • enzyme binding Source: RGD
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: RGD

GO - Biological processi

  • leukotriene biosynthetic process Source: RGD
  • leukotriene production involved in inflammatory response Source: Ensembl
  • lipoxygenase pathway Source: RGD
  • oxidation-reduction process Source: GOC
  • positive regulation of acute inflammatory response Source: RGD
  • protein homotrimerization Source: RGD

Keywordsi

Biological processLeukotriene biosynthesis

Enzyme and pathway databases

ReactomeiR-RNO-2142688. Synthesis of 5-eicosatetraenoic acids.
R-RNO-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-RNO-2142700. Synthesis of Lipoxins (LX).

Names & Taxonomyi

Protein namesi
Recommended name:
Arachidonate 5-lipoxygenase-activating protein
Alternative name(s):
FLAP
MK-886-binding protein
Gene namesi
Name:Alox5ap
Synonyms:Flap
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi2097. Alox5ap.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8LumenalBy similarity8
Transmembranei9 – 30HelicalBy similarityAdd BLAST22
Topological domaini31 – 52CytoplasmicBy similarityAdd BLAST22
Transmembranei53 – 77HelicalBy similarityAdd BLAST25
Topological domaini78 – 80LumenalBy similarity3
Transmembranei81 – 102HelicalBy similarityAdd BLAST22
Topological domaini103 – 107CytoplasmicBy similarity5
Intramembranei108 – 115By similarity8
Transmembranei116 – 128HelicalBy similarityAdd BLAST13
Topological domaini129 – 161LumenalBy similarityAdd BLAST33

GO - Cellular componenti

  • cytosol Source: RGD
  • endoplasmic reticulum Source: RGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: RGD
  • nuclear envelope Source: RGD
  • nuclear membrane Source: UniProtKB

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1921661.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002177561 – 161Arachidonate 5-lipoxygenase-activating proteinAdd BLAST161

Proteomic databases

PaxDbiP20291.
PRIDEiP20291.

PTM databases

PhosphoSitePlusiP20291.

Expressioni

Gene expression databases

BgeeiENSRNOG00000000907.
GenevisibleiP20291. RN.

Interactioni

Subunit structurei

Homotrimer. Interacts with LTC4S and ALOX5 (By similarity).By similarity

GO - Molecular functioni

  • enzyme binding Source: RGD
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

DIPiDIP-48660N.
STRINGi10116.ENSRNOP00000001207.

Chemistry databases

BindingDBiP20291.

Structurei

3D structure databases

ProteinModelPortaliP20291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 27Inhibitor bindingBy similarity8
Regioni112 – 123Inhibitor bindingBy similarityAdd BLAST12

Domaini

The C-terminal part after residue 140 is mostly disordered.By similarity

Sequence similaritiesi

Belongs to the MAPEG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHE0. Eukaryota.
ENOG4111T4I. LUCA.
GeneTreeiENSGT00430000030964.
HOGENOMiHOG000116372.
HOVERGENiHBG107295.
InParanoidiP20291.
KOiK20735.
OMAiNCMDAYP.
OrthoDBiEOG091G12NW.
PhylomeDBiP20291.
TreeFamiTF105328.

Family and domain databases

Gene3Di1.20.120.550. 1 hit.
InterProiView protein in InterPro
IPR001446. 5_LipOase_AP.
IPR018295. FLAP/GST2/LTC4S_CS.
IPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
PfamiView protein in Pfam
PF01124. MAPEG. 1 hit.
PRINTSiPR00488. 5LPOXGNASEAP.
PROSITEiView protein in PROSITE
PS01297. FLAP_GST2_LTC4S. 1 hit.

Sequencei

Sequence statusi: Complete.

P20291-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQEAVGNVV LLAIVTLISV VQNAFFAHKV ELESKAQSGR SFQRTGTLAF
60 70 80 90 100
ERVYTANQNC VDAYPTFLVV LWTAGLLCSQ VPAAFAGLMY LFVRQKYFVG
110 120 130 140 150
YLGERTQSTP GYIFGKRIIL FLFLMSLAGI LNHYLIFFFG SDFENYIRTI
160
TTTISPLLLI P
Length:161
Mass (Da):18,070
Last modified:May 1, 2007 - v2
Checksum:i9CCD6F8B3AA3B87E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32L → I AA sequence (PubMed:2300172).Curated1
Sequence conflicti127L → I AA sequence (PubMed:2300172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52196 mRNA. Translation: CAA36442.1.
BC086593 mRNA. Translation: AAH86593.1.
PIRiS08206.
RefSeqiNP_058956.1. NM_017260.2.
UniGeneiRn.18399.

Genome annotation databases

EnsembliENSRNOT00000001207; ENSRNOP00000001207; ENSRNOG00000000907.
GeneIDi29624.
KEGGirno:29624.
UCSCiRGD:2097. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiAL5AP_RAT
AccessioniPrimary (citable) accession number: P20291
Secondary accession number(s): Q5RJL3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: May 1, 2007
Last modified: May 10, 2017
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families