Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P20291 (AL5AP_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Arachidonate 5-lipoxygenase-activating protein
Alternative name(s):
FLAP
MK-886-binding protein
Gene names
Name:Alox5ap
Synonyms:Flap
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length161 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes. unstructured By similarity.

Subunit structure

Homotrimer. Interacts with LTC4S and ALOX5 By similarity.

Subcellular location

Nucleus membrane; Multi-pass membrane protein By similarity. Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Domain

The C-terminal part after residue 140 is mostly disordered By similarity.

Sequence similarities

Belongs to the MAPEG family.

Ontologies

Keywords
   Biological processLeukotriene biosynthesis
   Cellular componentEndoplasmic reticulum
Membrane
Nucleus
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to calcium ion

Inferred from electronic annotation. Source: Ensembl

leukotriene biosynthetic process

Inferred from direct assay PubMed 19075240Ref.1. Source: RGD

leukotriene production involved in inflammatory response

Inferred from electronic annotation. Source: Ensembl

lipoxygenase pathway

Inferred from direct assay PubMed 17517102. Source: RGD

oxidation-reduction process

Inferred from direct assay Ref.1. Source: GOC

positive regulation of acute inflammatory response

Inferred from mutant phenotype PubMed 20519143. Source: RGD

protein homotrimerization

Inferred from direct assay PubMed 15084748. Source: RGD

   Cellular_componentcytosol

Inferred from direct assay PubMed 8071328. Source: RGD

endoplasmic reticulum

Inferred from direct assay PubMed 8071328. Source: RGD

endoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

membrane

Traceable author statement PubMed 11468001. Source: RGD

nuclear envelope

Inferred from sequence or structural similarity. Source: UniProtKB

nuclear membrane

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionarachidonic acid binding

Inferred from sequence or structural similarity. Source: UniProtKB

enzyme activator activity

Inferred from electronic annotation. Source: InterPro

enzyme binding

Inferred from physical interaction PubMed 19075240. Source: RGD

protein heterodimerization activity

Inferred from physical interaction PubMed 15084748. Source: RGD

protein homodimerization activity

Inferred from direct assay PubMed 15084748. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 161161Arachidonate 5-lipoxygenase-activating protein
PRO_0000217756

Regions

Topological domain1 – 88Lumenal By similarity
Transmembrane9 – 3022Helical; By similarity
Topological domain31 – 5222Cytoplasmic By similarity
Transmembrane53 – 7725Helical; By similarity
Topological domain78 – 803Lumenal By similarity
Transmembrane81 – 10222Helical; By similarity
Topological domain103 – 1075Cytoplasmic By similarity
Intramembrane108 – 1158 By similarity
Transmembrane116 – 12813Helical; By similarity
Topological domain129 – 16133Lumenal By similarity
Region20 – 278Inhibitor binding By similarity
Region112 – 12312Inhibitor binding By similarity

Experimental info

Sequence conflict321L → I AA sequence Ref.3
Sequence conflict1271L → I AA sequence Ref.3

Sequences

Sequence LengthMass (Da)Tools
P20291 [UniParc].

Last modified May 1, 2007. Version 2.
Checksum: 9CCD6F8B3AA3B87E

FASTA16118,070
        10         20         30         40         50         60 
MDQEAVGNVV LLAIVTLISV VQNAFFAHKV ELESKAQSGR SFQRTGTLAF ERVYTANQNC 

        70         80         90        100        110        120 
VDAYPTFLVV LWTAGLLCSQ VPAAFAGLMY LFVRQKYFVG YLGERTQSTP GYIFGKRIIL 

       130        140        150        160 
FLFLMSLAGI LNHYLIFFFG SDFENYIRTI TTTISPLLLI P 

« Hide

References

« Hide 'large scale' references
[1]"Requirement of a 5-lipoxygenase-activating protein for leukotriene synthesis."
Dixon R.A.F., Diehl R.E., Opas E., Rands E., Vickers P.J., Evans J.F., Gillard J.W., Miller D.K.
Nature 343:282-284(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary.
[3]"Identification and isolation of a membrane protein necessary for leukotriene production."
Miller D.K., Gillard J.W., Vickers P.J., Sadowski S., Leveille C., Mancini J.A., Charleson P., Dixon R.A.F., Ford-Hutchinson A.W., Fortin R., Gauthier J.Y., Rodkey J., Rosen R., Rouzer C., Sigal I.S., Strader C.D., Evans J.F.
Nature 343:278-281(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-39; 90-121 AND 126-146.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X52196 mRNA. Translation: CAA36442.1.
BC086593 mRNA. Translation: AAH86593.1.
PIRS08206.
RefSeqNP_058956.1. NM_017260.2.
UniGeneRn.18399.

3D structure databases

ProteinModelPortalP20291.
SMRP20291. Positions 1-149.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-48660N.
STRING10116.ENSRNOP00000001207.

Chemistry

BindingDBP20291.
ChEMBLCHEMBL1921661.

PTM databases

PhosphoSiteP20291.

Proteomic databases

PRIDEP20291.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000001207; ENSRNOP00000001207; ENSRNOG00000000907.
GeneID29624.
KEGGrno:29624.
UCSCRGD:2097. rat.

Organism-specific databases

CTD241.
RGD2097. Alox5ap.

Phylogenomic databases

eggNOGNOG79474.
GeneTreeENSGT00430000030964.
HOGENOMHOG000116372.
HOVERGENHBG107295.
InParanoidP20291.
OMANCMDAYP.
OrthoDBEOG7GXPD5.
PhylomeDBP20291.
TreeFamTF105328.

Gene expression databases

GenevestigatorP20291.

Family and domain databases

Gene3D1.20.120.550. 1 hit.
InterProIPR001446. 5_LipOase_AP.
IPR018295. FLAP/GST2/LTC4S_CS.
IPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamPF01124. MAPEG. 1 hit.
[Graphical view]
PRINTSPR00488. 5LPOXGNASEAP.
PROSITEPS01297. FLAP_GST2_LTC4S. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio609834.
PROP20291.

Entry information

Entry nameAL5AP_RAT
AccessionPrimary (citable) accession number: P20291
Secondary accession number(s): Q5RJL3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: May 1, 2007
Last modified: April 16, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families